Investigação molecular de variantes genéticas do gene FTO na obesidade infantil

CARVALHO, Mariana Teixeira de

Investigação molecular de variantes genéticas do gene FTO na obesidade infantil

Detalhes bibliográficos
Autor(a) principal:	CARVALHO, Mariana Teixeira de
Data de Publicação:	2017
Tipo de documento:	Dissertação
Idioma:	por
Título da fonte:	Biblioteca Digital de Teses e Dissertações da UFTM
Texto Completo:	http://bdtd.uftm.edu.br/handle/tede/785
Resumo:	A prevalência da obesidade vem crescendo de forma expressiva no mundo todo, chegando a ser considerada, em muitos lugares, a maior epidemia de saúde pública e a principal causa de morte prematura. É definida como um acúmulo anormal ou excessivo de gordura que pode prejudicar a saúde. Inúmeros estudos têm avaliado genes e sua associação com a obesidade infantil. O gene FTO (fat mass and obesity associated) é um dos mais relacionados à obesidade, no qual sua superexpressão está relacionada ao aumento de peso nesta condição, podendo ser modulada por variantes genéticas. Este trabalho tem como objetivos investigar as frequências das variantes genéticas rs9940128, rs8050136, rs9939609 do gene FTO, em crianças com sobrepeso e obesidade e verificar associação destas e parâmetros como sexo, histórico de obesidade na família e atividade física com a obesidade infantil. Trata-se de um estudo do tipo caso-controle retrospectivo com 364 indivíduos, dos quais o grupo de estudo compreendeu 186 crianças e adolescentes entre 5 e 19 anos diagnosticadas com sobrepeso ou obesidade. Fizeram parte do grupo controle 178 adultos, os quais não tiveram sobrepeso ou obesidade na infância. Para a avaliação das variantes genéticas foi realizada a técnica de PCR em Tempo Real, PCR aleloespecífico e PCR-RFLP. Para comparar as distribuições das frequências alélicas e genotípicas entre os grupos e verificar se as distribuições genotípicas estavam em Equilíbrio de Hardy-Weinberg (EHW), foi utilizado o teste de Qui-Quadrado (2). Para a análise do modelo de herança e dos haplótipos, foi utilizado o programa SNPStats. O modelo de regressão logística múltipla foi utilizado para determinar o efeito das variáveis analisadas e o desenvolvimento de obesidade infantil, incluindo fatores sociodemográficos, sinais clínicos e dados moleculares. O nível de significância considerado foi de 5% (p≤ 0,05). A frequência do genótipo heterozigoto foi maior nos grupos estudados para as três variantes genéticas. Na análise de regressão logística, o parâmetro mãe com excesso de peso e presença do alelo A da variante genética rs9940128 foram estatisticamente significativos (0,0225 e 0,0439, respectivamente). Não foi encontrada associação das variantes genéticas rs9940128 G>A, rs8050136 A>C e rs9939609 A>T, dos haplótipos, modelos de herança mendeliana e os parâmetros estudados com a obesidade infantil, exceto para mãe com excesso de peso, o que pode conduzir a pesquisas acerca do papel da8 herdabilidade nesta doença e no monitoramento familiar de crianças que apresentam excesso de peso.

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spelling	Investigação molecular de variantes genéticas do gene FTO na obesidade infantilObesidade infantil.IMC.Gene FTO.Variantes genéticas.Haplótipo.rs9940128.rs8050136.rs9939609.Childhood obesity.BMI.FTO gene.Genetic variants.Haplotype.rs9940128.rs8050136.rs9939609.GenéticaGenética Humana e MédicaA prevalência da obesidade vem crescendo de forma expressiva no mundo todo, chegando a ser considerada, em muitos lugares, a maior epidemia de saúde pública e a principal causa de morte prematura. É definida como um acúmulo anormal ou excessivo de gordura que pode prejudicar a saúde. Inúmeros estudos têm avaliado genes e sua associação com a obesidade infantil. O gene FTO (fat mass and obesity associated) é um dos mais relacionados à obesidade, no qual sua superexpressão está relacionada ao aumento de peso nesta condição, podendo ser modulada por variantes genéticas. Este trabalho tem como objetivos investigar as frequências das variantes genéticas rs9940128, rs8050136, rs9939609 do gene FTO, em crianças com sobrepeso e obesidade e verificar associação destas e parâmetros como sexo, histórico de obesidade na família e atividade física com a obesidade infantil. Trata-se de um estudo do tipo caso-controle retrospectivo com 364 indivíduos, dos quais o grupo de estudo compreendeu 186 crianças e adolescentes entre 5 e 19 anos diagnosticadas com sobrepeso ou obesidade. Fizeram parte do grupo controle 178 adultos, os quais não tiveram sobrepeso ou obesidade na infância. Para a avaliação das variantes genéticas foi realizada a técnica de PCR em Tempo Real, PCR aleloespecífico e PCR-RFLP. Para comparar as distribuições das frequências alélicas e genotípicas entre os grupos e verificar se as distribuições genotípicas estavam em Equilíbrio de Hardy-Weinberg (EHW), foi utilizado o teste de Qui-Quadrado (2). Para a análise do modelo de herança e dos haplótipos, foi utilizado o programa SNPStats. O modelo de regressão logística múltipla foi utilizado para determinar o efeito das variáveis analisadas e o desenvolvimento de obesidade infantil, incluindo fatores sociodemográficos, sinais clínicos e dados moleculares. O nível de significância considerado foi de 5% (p≤ 0,05). A frequência do genótipo heterozigoto foi maior nos grupos estudados para as três variantes genéticas. Na análise de regressão logística, o parâmetro mãe com excesso de peso e presença do alelo A da variante genética rs9940128 foram estatisticamente significativos (0,0225 e 0,0439, respectivamente). Não foi encontrada associação das variantes genéticas rs9940128 G>A, rs8050136 A>C e rs9939609 A>T, dos haplótipos, modelos de herança mendeliana e os parâmetros estudados com a obesidade infantil, exceto para mãe com excesso de peso, o que pode conduzir a pesquisas acerca do papel da8 herdabilidade nesta doença e no monitoramento familiar de crianças que apresentam excesso de peso.The prevalence of obesity has been growing significantly worldwide, and has become, in many places, the largest public health epidemic and the leading cause of premature death. It is defined as an abnormal or excessive accumulation of fat that can be harmful to health. Many studies have evaluated genes and their association with childhood obesity. The FTO (fat mass and obesity associated) gene is one of the most related to obesity, in which its overexpression is related to weight gain in this condition, and can be modulated by genetic variants. This work aims to investigate the frequencies of genetic variants rs9940128, rs8050136, rs9939609 of FTO gene in overweight and obese children and to verify the association of these and parameters such as gender, history of obesity in family and physical activity with childhood obesity. It is a retrospective case-control study with 364 individuals, of which the study group comprised 186 children and adolescents between 5 and 19 years diagnosed as being overweight or obese. A total of 178 adults were included in the control group, who were not overweight or obese in childhood. For the evaluation of the genetic variants, real-time PCR technique, allele-specific PCR and PCR-RFLP were performed. To compare the distributions of allele and genotype frequencies between the groups and to verify if the genotypic distributions were in HardyWeinberg equilibrium (EHW), the Chi-Square test (2) was used. For the analysis of the inheritance model and the haplotypes, SNPStats program was used. The multiple logistic regression model was used to determine the effect of variables analyzed and the development of childhood obesity, including sociodemographic factors, clinical signs and molecular data. The significance level considered was 5% (p≤0.05). The frequency of the heterozygous genotype was higher in the studied groups for the three genetic variants. In the logistic regression analysis, the parameter mother with excess weight and presence of the allele A of the genetic variant rs9940128 were statistically significant (0.0225 and 0.0439, respectively). No association was found between the genetic variants rs9940128 G> A, rs8050136 A> C and rs9939609 A> T, haplotypes, mendelian inheritance models and the parameters studied with childhood obesity, except for overweight mothers, which may lead to research on the role of heritability in this disease and in the family monitoring of children who are overweight.Fundação de Amparo à Pesquisa do Estado de Minas GeraisCoordenação de Aperfeiçoamento de Pessoal de Nível SuperiorConselho Nacional de Desenvolvimento Científico e TecnológicoUniversidade Federal do Triângulo MineiroInstituto de Ciências da Saúde - ICS::Programa de Pós-Graduação em Ciências da SaúdeBrasilUFTMPrograma de Pós-Graduação em Ciências da SaúdeBALARIN, Marly Aparecida Spadotto06268176847http://lattes.cnpq.br/9825231661876909GRECCO, Roseane Lopes da Silva07153828885CARVALHO, Mariana Teixeira de2019-07-22T17:28:26Z2017-08-03info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfCARVALHO, Mariana Teixeira de. 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dc.title.none.fl_str_mv	Investigação molecular de variantes genéticas do gene FTO na obesidade infantil
title	Investigação molecular de variantes genéticas do gene FTO na obesidade infantil
spellingShingle	Investigação molecular de variantes genéticas do gene FTO na obesidade infantil CARVALHO, Mariana Teixeira de Obesidade infantil. IMC. Gene FTO. Variantes genéticas. Haplótipo. rs9940128. rs8050136. rs9939609. Childhood obesity. BMI. FTO gene. Genetic variants. Haplotype. rs9940128. rs8050136. rs9939609. Genética Genética Humana e Médica
title_short	Investigação molecular de variantes genéticas do gene FTO na obesidade infantil
title_full	Investigação molecular de variantes genéticas do gene FTO na obesidade infantil
title_fullStr	Investigação molecular de variantes genéticas do gene FTO na obesidade infantil
title_full_unstemmed	Investigação molecular de variantes genéticas do gene FTO na obesidade infantil
title_sort	Investigação molecular de variantes genéticas do gene FTO na obesidade infantil
author	CARVALHO, Mariana Teixeira de
author_facet	CARVALHO, Mariana Teixeira de
author_role	author
dc.contributor.none.fl_str_mv	BALARIN, Marly Aparecida Spadotto 06268176847 http://lattes.cnpq.br/9825231661876909 GRECCO, Roseane Lopes da Silva 07153828885
dc.contributor.author.fl_str_mv	CARVALHO, Mariana Teixeira de
dc.subject.por.fl_str_mv	Obesidade infantil. IMC. Gene FTO. Variantes genéticas. Haplótipo. rs9940128. rs8050136. rs9939609. Childhood obesity. BMI. FTO gene. Genetic variants. Haplotype. rs9940128. rs8050136. rs9939609. Genética Genética Humana e Médica
topic	Obesidade infantil. IMC. Gene FTO. Variantes genéticas. Haplótipo. rs9940128. rs8050136. rs9939609. Childhood obesity. BMI. FTO gene. Genetic variants. Haplotype. rs9940128. rs8050136. rs9939609. Genética Genética Humana e Médica
description	A prevalência da obesidade vem crescendo de forma expressiva no mundo todo, chegando a ser considerada, em muitos lugares, a maior epidemia de saúde pública e a principal causa de morte prematura. É definida como um acúmulo anormal ou excessivo de gordura que pode prejudicar a saúde. Inúmeros estudos têm avaliado genes e sua associação com a obesidade infantil. O gene FTO (fat mass and obesity associated) é um dos mais relacionados à obesidade, no qual sua superexpressão está relacionada ao aumento de peso nesta condição, podendo ser modulada por variantes genéticas. Este trabalho tem como objetivos investigar as frequências das variantes genéticas rs9940128, rs8050136, rs9939609 do gene FTO, em crianças com sobrepeso e obesidade e verificar associação destas e parâmetros como sexo, histórico de obesidade na família e atividade física com a obesidade infantil. Trata-se de um estudo do tipo caso-controle retrospectivo com 364 indivíduos, dos quais o grupo de estudo compreendeu 186 crianças e adolescentes entre 5 e 19 anos diagnosticadas com sobrepeso ou obesidade. Fizeram parte do grupo controle 178 adultos, os quais não tiveram sobrepeso ou obesidade na infância. Para a avaliação das variantes genéticas foi realizada a técnica de PCR em Tempo Real, PCR aleloespecífico e PCR-RFLP. Para comparar as distribuições das frequências alélicas e genotípicas entre os grupos e verificar se as distribuições genotípicas estavam em Equilíbrio de Hardy-Weinberg (EHW), foi utilizado o teste de Qui-Quadrado (2). Para a análise do modelo de herança e dos haplótipos, foi utilizado o programa SNPStats. O modelo de regressão logística múltipla foi utilizado para determinar o efeito das variáveis analisadas e o desenvolvimento de obesidade infantil, incluindo fatores sociodemográficos, sinais clínicos e dados moleculares. O nível de significância considerado foi de 5% (p≤ 0,05). A frequência do genótipo heterozigoto foi maior nos grupos estudados para as três variantes genéticas. Na análise de regressão logística, o parâmetro mãe com excesso de peso e presença do alelo A da variante genética rs9940128 foram estatisticamente significativos (0,0225 e 0,0439, respectivamente). Não foi encontrada associação das variantes genéticas rs9940128 G>A, rs8050136 A>C e rs9939609 A>T, dos haplótipos, modelos de herança mendeliana e os parâmetros estudados com a obesidade infantil, exceto para mãe com excesso de peso, o que pode conduzir a pesquisas acerca do papel da8 herdabilidade nesta doença e no monitoramento familiar de crianças que apresentam excesso de peso.
publishDate	2017
dc.date.none.fl_str_mv	2017-08-03 2019-07-22T17:28:26Z
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv	info:eu-repo/semantics/masterThesis
format	masterThesis
status_str	publishedVersion
dc.identifier.uri.fl_str_mv	CARVALHO, Mariana Teixeira de. Investigação molecular de variantes genéticas do gene FTO na obesidade infantil. 2017. 85f. Dissertação (Mestrado em Ciências da Saúde) - Programa de Pós-Graduação em Ciências da Saúde, Universidade Federal do Triângulo Mineiro, Uberaba, 2017. http://bdtd.uftm.edu.br/handle/tede/785
identifier_str_mv	CARVALHO, Mariana Teixeira de. Investigação molecular de variantes genéticas do gene FTO na obesidade infantil. 2017. 85f. Dissertação (Mestrado em Ciências da Saúde) - Programa de Pós-Graduação em Ciências da Saúde, Universidade Federal do Triângulo Mineiro, Uberaba, 2017.
url	http://bdtd.uftm.edu.br/handle/tede/785
dc.language.iso.fl_str_mv	por
language	por
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Investigação molecular de variantes genéticas do gene FTO na obesidade infantil

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