Comparison of microscopic agglutination test and indirect elisa in the diagnosis of bovine leptospirosis
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Bioscience journal (Online) |
Texto Completo: | https://seer.ufu.br/index.php/biosciencejournal/article/view/18256 |
Resumo: | The objective of this research was to compare the results obtained by the Microscopic Agglutination Test (MAT) and indirect ELISA using outer membrane proteins (OMP) of the serovar Hardjo as antigen (ELISA/OMP-Hardjo). Ninety-three samples of blood serum from 3 to 6-year-old cattle of both sexes with no history of vaccination for leptospirosis were used. To perform the MAT, live cultures of 14 Leptospira serovars representing 11 serogroups of L. interrogans were used as antigen. Outer membrane proteins (OMP) of the serovar Hardjo (ELISA-OMP/Hardjo) were used as the ELISA antigen. Of the 93 samples tested by MAT, 37 (40%) were positive. None of the samples testing positive in the MAT were negative in the ELISA. Sensitivity was 100% and specificity 64%. A comparison of the MAT and ELISA-OMP/Hardjo tests showed 78% agreement and the Kappa index was 0.58 (p<0.0001). The ELISA-OMP/Hardjo proved to be a sensitive test for the diagnosis of bovine leptospirosis, indicating its potential use as a screening test and for epidemiological studies. The titration results obtained by MAT and by optical density (OD) in the ELISA-OMP/Hardjo test showed a positive correlation, and as the antibody titers in the MAT increased, so did the OD in ELISA, demonstrating the correspondence between the tests evaluated here. |
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Bioscience journal (Online) |
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Comparison of microscopic agglutination test and indirect elisa in the diagnosis of bovine leptospirosis Agricultural SciencesThe objective of this research was to compare the results obtained by the Microscopic Agglutination Test (MAT) and indirect ELISA using outer membrane proteins (OMP) of the serovar Hardjo as antigen (ELISA/OMP-Hardjo). Ninety-three samples of blood serum from 3 to 6-year-old cattle of both sexes with no history of vaccination for leptospirosis were used. To perform the MAT, live cultures of 14 Leptospira serovars representing 11 serogroups of L. interrogans were used as antigen. Outer membrane proteins (OMP) of the serovar Hardjo (ELISA-OMP/Hardjo) were used as the ELISA antigen. Of the 93 samples tested by MAT, 37 (40%) were positive. None of the samples testing positive in the MAT were negative in the ELISA. Sensitivity was 100% and specificity 64%. A comparison of the MAT and ELISA-OMP/Hardjo tests showed 78% agreement and the Kappa index was 0.58 (p<0.0001). The ELISA-OMP/Hardjo proved to be a sensitive test for the diagnosis of bovine leptospirosis, indicating its potential use as a screening test and for epidemiological studies. The titration results obtained by MAT and by optical density (OD) in the ELISA-OMP/Hardjo test showed a positive correlation, and as the antibody titers in the MAT increased, so did the OD in ELISA, demonstrating the correspondence between the tests evaluated here.EDUFU2014-09-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/18256Bioscience Journal ; Vol. 30 (2014): Supplement 2; 833-838Bioscience Journal ; v. 30 (2014): Supplement 2; 833-8381981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUporhttps://seer.ufu.br/index.php/biosciencejournal/article/view/18256/15272Brazil; ContemporanyCopyright (c) 2014 Mariana Assunção Souza, Jacqueline Ribeiro de Castro, Tatiane Cristina Fernandes Tavares, Pollyanna Mafra Soares, Mariane Pacheco dos Santos, Muriell Ribeiro Ganda, Dayane Olímpia Gomes, Anna Monteiro Correia Lima-Ribeirohttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSouza, Mariana AssunçãoCastro, Jacqueline Ribeiro deTavares, Tatiane Cristina FernandesSoares, Pollyanna MafraSantos, Mariane Pacheco dosGanda, Muriell RibeiroGomes, Dayane OlímpiaLima-Ribeiro, Anna Monteiro Correia2022-05-18T17:49:42Zoai:ojs.www.seer.ufu.br:article/18256Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2022-05-18T17:49:42Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false |
dc.title.none.fl_str_mv |
Comparison of microscopic agglutination test and indirect elisa in the diagnosis of bovine leptospirosis |
title |
Comparison of microscopic agglutination test and indirect elisa in the diagnosis of bovine leptospirosis |
spellingShingle |
Comparison of microscopic agglutination test and indirect elisa in the diagnosis of bovine leptospirosis Souza, Mariana Assunção Agricultural Sciences |
title_short |
Comparison of microscopic agglutination test and indirect elisa in the diagnosis of bovine leptospirosis |
title_full |
Comparison of microscopic agglutination test and indirect elisa in the diagnosis of bovine leptospirosis |
title_fullStr |
Comparison of microscopic agglutination test and indirect elisa in the diagnosis of bovine leptospirosis |
title_full_unstemmed |
Comparison of microscopic agglutination test and indirect elisa in the diagnosis of bovine leptospirosis |
title_sort |
Comparison of microscopic agglutination test and indirect elisa in the diagnosis of bovine leptospirosis |
author |
Souza, Mariana Assunção |
author_facet |
Souza, Mariana Assunção Castro, Jacqueline Ribeiro de Tavares, Tatiane Cristina Fernandes Soares, Pollyanna Mafra Santos, Mariane Pacheco dos Ganda, Muriell Ribeiro Gomes, Dayane Olímpia Lima-Ribeiro, Anna Monteiro Correia |
author_role |
author |
author2 |
Castro, Jacqueline Ribeiro de Tavares, Tatiane Cristina Fernandes Soares, Pollyanna Mafra Santos, Mariane Pacheco dos Ganda, Muriell Ribeiro Gomes, Dayane Olímpia Lima-Ribeiro, Anna Monteiro Correia |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Souza, Mariana Assunção Castro, Jacqueline Ribeiro de Tavares, Tatiane Cristina Fernandes Soares, Pollyanna Mafra Santos, Mariane Pacheco dos Ganda, Muriell Ribeiro Gomes, Dayane Olímpia Lima-Ribeiro, Anna Monteiro Correia |
dc.subject.por.fl_str_mv |
Agricultural Sciences |
topic |
Agricultural Sciences |
description |
The objective of this research was to compare the results obtained by the Microscopic Agglutination Test (MAT) and indirect ELISA using outer membrane proteins (OMP) of the serovar Hardjo as antigen (ELISA/OMP-Hardjo). Ninety-three samples of blood serum from 3 to 6-year-old cattle of both sexes with no history of vaccination for leptospirosis were used. To perform the MAT, live cultures of 14 Leptospira serovars representing 11 serogroups of L. interrogans were used as antigen. Outer membrane proteins (OMP) of the serovar Hardjo (ELISA-OMP/Hardjo) were used as the ELISA antigen. Of the 93 samples tested by MAT, 37 (40%) were positive. None of the samples testing positive in the MAT were negative in the ELISA. Sensitivity was 100% and specificity 64%. A comparison of the MAT and ELISA-OMP/Hardjo tests showed 78% agreement and the Kappa index was 0.58 (p<0.0001). The ELISA-OMP/Hardjo proved to be a sensitive test for the diagnosis of bovine leptospirosis, indicating its potential use as a screening test and for epidemiological studies. The titration results obtained by MAT and by optical density (OD) in the ELISA-OMP/Hardjo test showed a positive correlation, and as the antibody titers in the MAT increased, so did the OD in ELISA, demonstrating the correspondence between the tests evaluated here. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-09-30 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/18256 |
url |
https://seer.ufu.br/index.php/biosciencejournal/article/view/18256 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/18256/15272 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
Brazil; Contemporany |
dc.publisher.none.fl_str_mv |
EDUFU |
publisher.none.fl_str_mv |
EDUFU |
dc.source.none.fl_str_mv |
Bioscience Journal ; Vol. 30 (2014): Supplement 2; 833-838 Bioscience Journal ; v. 30 (2014): Supplement 2; 833-838 1981-3163 reponame:Bioscience journal (Online) instname:Universidade Federal de Uberlândia (UFU) instacron:UFU |
instname_str |
Universidade Federal de Uberlândia (UFU) |
instacron_str |
UFU |
institution |
UFU |
reponame_str |
Bioscience journal (Online) |
collection |
Bioscience journal (Online) |
repository.name.fl_str_mv |
Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU) |
repository.mail.fl_str_mv |
biosciencej@ufu.br|| |
_version_ |
1797069073585012736 |