Alternative products for controlling Sclerotinia sclerotiorum in vivo AND in vitro in canola crops
Autor(a) principal: | |
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Data de Publicação: | 2024 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Bioscience journal (Online) |
Texto Completo: | https://seer.ufu.br/index.php/biosciencejournal/article/view/53799 |
Resumo: | Products that activate plant defense mechanisms, such as white mold, may increase the disease control spectrum in canola crops as an alternative to chemical control for disease management, aiming at lower environmental impacts and selection pressure on pathogens. This study evaluated Sclerotinia sclerotiorum control and resistance induction in canola crops with alternative products. In vitro and detached leaf experiments were conducted in a laboratory, and in vivo experiments occurred in a greenhouse. High Roots®; V6®; Maxi Flor®; Wert Plus®; potassium, manganese, and copper phosphites; manganese; procymidone fungicide; and Ascophyllum nodosum, Bacillus subtilis, and Bacillus thuringiensis extracts assessed S. sclerotiorum mycelial growth in vitro in detached leaves, white mold severity in a greenhouse, and enzymatic analysis in leaf tissues. The last two experiments also received the acibenzolar-S-methyl and Trichoderma asperellum treatments. S. sclerotiorum mycelial growth showed 100% inhibition under B. subtilis and Wert Plus® at 1000 ppm and procymidone at 100 and 1000 ppm in vitro. The procymidone and B. subtilis treatments in detached leaves inhibited 100% of fungus growth. The greenhouse experiments with lower severity were procymidone, copper phosphite, B. thuringiensis, B. subtilis, manganese phosphite, potassium phosphite, High Roots®, and V6®. Higher peroxidase enzyme activity occurred in plants treated with acibenzolar-S-methyl, copper phosphite, and fungicide 48 hours after pathogen inoculation. The polyphenol oxidase enzyme did not show activity. Alternative products were responsive and may aid the chemical control of white mold in canola crops. |
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Alternative products for controlling Sclerotinia sclerotiorum in vivo AND in vitro in canola cropsBiological controlBrassica napusEnzymatic activity. Agricultural SciencesInglêsProducts that activate plant defense mechanisms, such as white mold, may increase the disease control spectrum in canola crops as an alternative to chemical control for disease management, aiming at lower environmental impacts and selection pressure on pathogens. This study evaluated Sclerotinia sclerotiorum control and resistance induction in canola crops with alternative products. In vitro and detached leaf experiments were conducted in a laboratory, and in vivo experiments occurred in a greenhouse. High Roots®; V6®; Maxi Flor®; Wert Plus®; potassium, manganese, and copper phosphites; manganese; procymidone fungicide; and Ascophyllum nodosum, Bacillus subtilis, and Bacillus thuringiensis extracts assessed S. sclerotiorum mycelial growth in vitro in detached leaves, white mold severity in a greenhouse, and enzymatic analysis in leaf tissues. The last two experiments also received the acibenzolar-S-methyl and Trichoderma asperellum treatments. S. sclerotiorum mycelial growth showed 100% inhibition under B. subtilis and Wert Plus® at 1000 ppm and procymidone at 100 and 1000 ppm in vitro. The procymidone and B. subtilis treatments in detached leaves inhibited 100% of fungus growth. The greenhouse experiments with lower severity were procymidone, copper phosphite, B. thuringiensis, B. subtilis, manganese phosphite, potassium phosphite, High Roots®, and V6®. Higher peroxidase enzyme activity occurred in plants treated with acibenzolar-S-methyl, copper phosphite, and fungicide 48 hours after pathogen inoculation. The polyphenol oxidase enzyme did not show activity. Alternative products were responsive and may aid the chemical control of white mold in canola crops.Em busca de alternativas ao controle químico para manejar doenças, visando menores impactos ambientais e pressão de seleção sobre os patógenos, o uso de produtos que ativam mecanismos de defesa das plantas podem aumentar o espectro de controle das doenças na cultura da canola, como o mofo-branco. O objetivo do trabalho foi avaliar o controle de Sclerotinia sclerotiorum e indução de resistência na cultura da canola utilizando-se produtos alternativos. Experimentos in vitro e de folha destacada foram conduzidos em laboratório e os in vivo em casa de vegetação. Os tratamentos Hight Roots®; V6®; Maxi Flor®; Wert Plus®, fosfito de potássio; fosfito de manganês; fosfito de cobre, manganês, fungicida procimidona, extrato de Ascophyllum nodosum, Bacillus subtilis e Bacillus thuringiensis foram utilizados para avaliar o crescimento micelial in vitro, em folha destacada, severidade da doença em casa de vegetação e também análise enzimática nos tecidos foliares. Para os dois últimos experimentos adicionou-se os tratamentos acibenzolar-S-metílico e Trichoderma asperellum. Verificou-se 100% de inibição do crescimento micelial de S. sclerotiorum nos tratamentos B. subtillis, Wert Plus® na concentração de 1000 ppm, e procimidona na concentração de 100 e 1000 ppm in vitro. Em folha destacada os tratamentos procimidona e B. subtillis inibiram 100% o crescimento do fungo. Em casa de vegetação os tratamentos com menores valores de severidade, foram: procimidona, fosfito de cobre, B. thuringiensis, B. subtillis, fosfito de manganês, fosfito de potássio, fosfito de cobre, High Roots® e V6®, respectivamente. A maior atividade da enzima peroxidase foi observada em plantas tratadas com acibenzolar-S-metílico, fosfito de cobre e fungicida 48 horas após inoculação do fungo. Não houve atividade da enzima polifenoloxidase. A utilização de produtos alternativos é responsiva e pode ser utilizada como auxiliar no controle químico do mofo-branco na cultura da canola. PALAVRAS-CHAVE: Brassica napus; controle biológico; atividade enzimática.Universidade Federal de Uberlândia2024-03-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/5379910.14393/BJ-v40n0a2024-53799Bioscience Journal ; Vol. 40 (2024): Continuous Publication; e40013Bioscience Journal ; v. 40 (2024): Continuous Publication; e400131981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/53799/38397Brazil; Contemporary Copyright (c) 2024 Hagata Siqueira Henipman, Maristella Dalla Pria, Polyana Elvira Christmann Rinaldi, Filipe Lemos Jacques, Letícia Reishttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccess Henipman, Hagata Siqueira Pria, Maristella Dalla Rinaldi, Polyana Elvira Christmann Jacques, Filipe LemosReis, Letícia2024-04-03T20:47:42Zoai:ojs.www.seer.ufu.br:article/53799Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2024-04-03T20:47:42Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false |
dc.title.none.fl_str_mv |
Alternative products for controlling Sclerotinia sclerotiorum in vivo AND in vitro in canola crops |
title |
Alternative products for controlling Sclerotinia sclerotiorum in vivo AND in vitro in canola crops |
spellingShingle |
Alternative products for controlling Sclerotinia sclerotiorum in vivo AND in vitro in canola crops Henipman, Hagata Siqueira Biological control Brassica napus Enzymatic activity. Agricultural Sciences Inglês |
title_short |
Alternative products for controlling Sclerotinia sclerotiorum in vivo AND in vitro in canola crops |
title_full |
Alternative products for controlling Sclerotinia sclerotiorum in vivo AND in vitro in canola crops |
title_fullStr |
Alternative products for controlling Sclerotinia sclerotiorum in vivo AND in vitro in canola crops |
title_full_unstemmed |
Alternative products for controlling Sclerotinia sclerotiorum in vivo AND in vitro in canola crops |
title_sort |
Alternative products for controlling Sclerotinia sclerotiorum in vivo AND in vitro in canola crops |
author |
Henipman, Hagata Siqueira |
author_facet |
Henipman, Hagata Siqueira Pria, Maristella Dalla Rinaldi, Polyana Elvira Christmann Jacques, Filipe Lemos Reis, Letícia |
author_role |
author |
author2 |
Pria, Maristella Dalla Rinaldi, Polyana Elvira Christmann Jacques, Filipe Lemos Reis, Letícia |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Henipman, Hagata Siqueira Pria, Maristella Dalla Rinaldi, Polyana Elvira Christmann Jacques, Filipe Lemos Reis, Letícia |
dc.subject.por.fl_str_mv |
Biological control Brassica napus Enzymatic activity. Agricultural Sciences Inglês |
topic |
Biological control Brassica napus Enzymatic activity. Agricultural Sciences Inglês |
description |
Products that activate plant defense mechanisms, such as white mold, may increase the disease control spectrum in canola crops as an alternative to chemical control for disease management, aiming at lower environmental impacts and selection pressure on pathogens. This study evaluated Sclerotinia sclerotiorum control and resistance induction in canola crops with alternative products. In vitro and detached leaf experiments were conducted in a laboratory, and in vivo experiments occurred in a greenhouse. High Roots®; V6®; Maxi Flor®; Wert Plus®; potassium, manganese, and copper phosphites; manganese; procymidone fungicide; and Ascophyllum nodosum, Bacillus subtilis, and Bacillus thuringiensis extracts assessed S. sclerotiorum mycelial growth in vitro in detached leaves, white mold severity in a greenhouse, and enzymatic analysis in leaf tissues. The last two experiments also received the acibenzolar-S-methyl and Trichoderma asperellum treatments. S. sclerotiorum mycelial growth showed 100% inhibition under B. subtilis and Wert Plus® at 1000 ppm and procymidone at 100 and 1000 ppm in vitro. The procymidone and B. subtilis treatments in detached leaves inhibited 100% of fungus growth. The greenhouse experiments with lower severity were procymidone, copper phosphite, B. thuringiensis, B. subtilis, manganese phosphite, potassium phosphite, High Roots®, and V6®. Higher peroxidase enzyme activity occurred in plants treated with acibenzolar-S-methyl, copper phosphite, and fungicide 48 hours after pathogen inoculation. The polyphenol oxidase enzyme did not show activity. Alternative products were responsive and may aid the chemical control of white mold in canola crops. |
publishDate |
2024 |
dc.date.none.fl_str_mv |
2024-03-15 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/53799 10.14393/BJ-v40n0a2024-53799 |
url |
https://seer.ufu.br/index.php/biosciencejournal/article/view/53799 |
identifier_str_mv |
10.14393/BJ-v40n0a2024-53799 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/53799/38397 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
Brazil; Contemporary |
dc.publisher.none.fl_str_mv |
Universidade Federal de Uberlândia |
publisher.none.fl_str_mv |
Universidade Federal de Uberlândia |
dc.source.none.fl_str_mv |
Bioscience Journal ; Vol. 40 (2024): Continuous Publication; e40013 Bioscience Journal ; v. 40 (2024): Continuous Publication; e40013 1981-3163 reponame:Bioscience journal (Online) instname:Universidade Federal de Uberlândia (UFU) instacron:UFU |
instname_str |
Universidade Federal de Uberlândia (UFU) |
instacron_str |
UFU |
institution |
UFU |
reponame_str |
Bioscience journal (Online) |
collection |
Bioscience journal (Online) |
repository.name.fl_str_mv |
Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU) |
repository.mail.fl_str_mv |
biosciencej@ufu.br|| |
_version_ |
1797069065094692864 |