Evaluation of the serum biochemistry and histopathology of kidney and bladder of dogs with Leishmania sp. in their urine

Detalhes bibliográficos
Autor(a) principal: Batista, Joilson Ferreira
Data de Publicação: 2020
Outros Autores: Verçosa, Barbara Laurice Araújo, Alves, Michel Muálem de Moraes, Rocha, Fernanda Samara Barbosa, Carvalho, Rayssa Maria de Araújo, Prianti, Maria das Graças, Queiroz, Bárbara Cristina Silva Holanda, Costa, Carlos Henrique Nery, Mendonça, Ivete Lopes de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Bioscience journal (Online)
Texto Completo: https://seer.ufu.br/index.php/biosciencejournal/article/view/42464
Resumo: The visceral establishment of Leishmania infantum in dogs may result in kidney and bladder tissue injury, with L. infantum ending up in urine. This study therefore aimed at investigating the presence of Leishmania sp. in urinary sediments, and correlating the results with those from renal and bladder serum biochemistry and histopathology. Thirty dogs with negative Nested-Polymerase Chain Reaction (PCR) for E. canis were used in the experiment, and were divided into three groups: control group (10 dogs), neither leishmaniasis nor clinical changes; group I (15 dogs), leishmaniasis but no Leishmania sp. in urine; and group II (5 dogs), leishmaniasis, as well as Leishmania sp. in urine. All animals were submitted to clinical, serological, and parasitological diagnosis for leishmaniasis, biochemical exams, and kidney and bladder histopathology. The parasite was also detected in the bladder imprint of one group II dog. Group II dogs presented with very low albumin concentrations, low albumin/globulin ratios, and kidney and bladder lesions. In the kidneys, hydropic degeneration, thickened Bowman's capsule, and thickening of the tubular capsule were detected in all dogs with positive urinary sediment. However, no significant difference in these renal changes was observed between groups. The intensity and distribution of bladder inflammatory infiltrates were significantly (p-value < 0.05, Kruskal-Wallis’ and Dunn’s tests) higher in group II dogs, compared with those of the other groups. The presence of Leishmania sp. in the urine of infected dogs appeared to be related to low serum albumin concentrations and more severe bladder lesions.
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spelling Evaluation of the serum biochemistry and histopathology of kidney and bladder of dogs with Leishmania sp. in their urineAvaliação da bioquímica sérica e da histopatologia de rim e bexiga em cães com Leishmania sp. na urinaHistopathologyLeishmaniasisSerum biochemistryUrine.Agricultural SciencesHistopatologiaLeishmanioseBioquímica séricaUrinaThe visceral establishment of Leishmania infantum in dogs may result in kidney and bladder tissue injury, with L. infantum ending up in urine. This study therefore aimed at investigating the presence of Leishmania sp. in urinary sediments, and correlating the results with those from renal and bladder serum biochemistry and histopathology. Thirty dogs with negative Nested-Polymerase Chain Reaction (PCR) for E. canis were used in the experiment, and were divided into three groups: control group (10 dogs), neither leishmaniasis nor clinical changes; group I (15 dogs), leishmaniasis but no Leishmania sp. in urine; and group II (5 dogs), leishmaniasis, as well as Leishmania sp. in urine. All animals were submitted to clinical, serological, and parasitological diagnosis for leishmaniasis, biochemical exams, and kidney and bladder histopathology. The parasite was also detected in the bladder imprint of one group II dog. Group II dogs presented with very low albumin concentrations, low albumin/globulin ratios, and kidney and bladder lesions. In the kidneys, hydropic degeneration, thickened Bowman's capsule, and thickening of the tubular capsule were detected in all dogs with positive urinary sediment. However, no significant difference in these renal changes was observed between groups. The intensity and distribution of bladder inflammatory infiltrates were significantly (p-value < 0.05, Kruskal-Wallis’ and Dunn’s tests) higher in group II dogs, compared with those of the other groups. The presence of Leishmania sp. in the urine of infected dogs appeared to be related to low serum albumin concentrations and more severe bladder lesions.O estabelecimento visceral de Leishmania infantum em cães pode resultar em lesões nos tecidos dos rins e da bexiga, favorecendo a chegando do parasito até a urina. Portanto, este estudo teve como objetivo investigar a presença de Leishmania sp. em sedimentos urinários e correlacionar os resultados com os achados de quantificações bioquímicas séricas e histopatologia de rim e bexiga. Trinta cães com Nested-Reação em Cadeia da Polimerase (PCR) negativa para E. canis foram utilizados no experimento e foram divididos em três grupos: grupo controle (10 cães), negativos para leishmaniose e sem alterações clínicas; grupo I (15 cães), com leishmaniose, mas sem Leishmania sp. na urina; e grupo II (5 cães), com leishmaniose e com Leishmania sp. na urina. Todos os animais foram submetidos a diagnóstico clínico, sorológico e parasitológico para leishmaniose, exames bioquímicos e histopatologia de rim e bexiga. O parasito foi detectado no imprimt de bexiga de um cão do grupo II. Os cães do grupo II apresentaram concentrações muito baixas de albumina, baixa relação albumina/globulina e lesões nos rins e na bexiga. Nos rins, foram detectadas degeneração hidrópica, espessamento da cápsula de Bowman e espessamento da cápsula tubular, em todos os cães com sedimento urinário positivo. No entanto, nenhuma diferença significativa nessas alterações renais foi observada entre os grupos. A intensidade e a distribuição dos infiltrados inflamatórios da bexiga foram significativamente (p-valor < 0,05, testes de Kruskal-Wallis e Dunn) maiores nos cães do grupo II, em comparação com a dos outros grupos. A presença de Leishmania sp. na urina de cães infectados parece estar relacionada a baixa concentração sérica de albumina e a lesões mais graves na bexiga.EDUFU2020-04-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/4246410.14393/BJ-v36n3a2020-42464Bioscience Journal ; Vol. 36 No. 3 (2020): May/June; 956-967Bioscience Journal ; v. 36 n. 3 (2020): Maio/Junho; 956-9671981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/42464/28647Brazil; ContemporaryCopyright (c) 2020 Joilson Ferreira Batista, Barbara Laurice Araújo Verçosa, Michel Muálem de Moraes Alves, Fernanda Samara Barbosa Rocha, Rayssa Maria de Araújo Carvalho, Maria das Graças Prianti, Bárbara Cristina Silva Holanda Queiroz, Carlos Henrique Nery Costa, Ivete Lopes de Mendonçahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessBatista, Joilson FerreiraVerçosa, Barbara Laurice Araújo Alves, Michel Muálem de MoraesRocha, Fernanda Samara Barbosa Carvalho, Rayssa Maria de Araújo Prianti, Maria das GraçasQueiroz, Bárbara Cristina Silva Holanda Costa, Carlos Henrique Nery Mendonça, Ivete Lopes de2022-06-20T13:16:24Zoai:ojs.www.seer.ufu.br:article/42464Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2022-06-20T13:16:24Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false
dc.title.none.fl_str_mv Evaluation of the serum biochemistry and histopathology of kidney and bladder of dogs with Leishmania sp. in their urine
Avaliação da bioquímica sérica e da histopatologia de rim e bexiga em cães com Leishmania sp. na urina
title Evaluation of the serum biochemistry and histopathology of kidney and bladder of dogs with Leishmania sp. in their urine
spellingShingle Evaluation of the serum biochemistry and histopathology of kidney and bladder of dogs with Leishmania sp. in their urine
Batista, Joilson Ferreira
Histopathology
Leishmaniasis
Serum biochemistry
Urine.
Agricultural Sciences
Histopatologia
Leishmaniose
Bioquímica sérica
Urina
title_short Evaluation of the serum biochemistry and histopathology of kidney and bladder of dogs with Leishmania sp. in their urine
title_full Evaluation of the serum biochemistry and histopathology of kidney and bladder of dogs with Leishmania sp. in their urine
title_fullStr Evaluation of the serum biochemistry and histopathology of kidney and bladder of dogs with Leishmania sp. in their urine
title_full_unstemmed Evaluation of the serum biochemistry and histopathology of kidney and bladder of dogs with Leishmania sp. in their urine
title_sort Evaluation of the serum biochemistry and histopathology of kidney and bladder of dogs with Leishmania sp. in their urine
author Batista, Joilson Ferreira
author_facet Batista, Joilson Ferreira
Verçosa, Barbara Laurice Araújo
Alves, Michel Muálem de Moraes
Rocha, Fernanda Samara Barbosa
Carvalho, Rayssa Maria de Araújo
Prianti, Maria das Graças
Queiroz, Bárbara Cristina Silva Holanda
Costa, Carlos Henrique Nery
Mendonça, Ivete Lopes de
author_role author
author2 Verçosa, Barbara Laurice Araújo
Alves, Michel Muálem de Moraes
Rocha, Fernanda Samara Barbosa
Carvalho, Rayssa Maria de Araújo
Prianti, Maria das Graças
Queiroz, Bárbara Cristina Silva Holanda
Costa, Carlos Henrique Nery
Mendonça, Ivete Lopes de
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Batista, Joilson Ferreira
Verçosa, Barbara Laurice Araújo
Alves, Michel Muálem de Moraes
Rocha, Fernanda Samara Barbosa
Carvalho, Rayssa Maria de Araújo
Prianti, Maria das Graças
Queiroz, Bárbara Cristina Silva Holanda
Costa, Carlos Henrique Nery
Mendonça, Ivete Lopes de
dc.subject.por.fl_str_mv Histopathology
Leishmaniasis
Serum biochemistry
Urine.
Agricultural Sciences
Histopatologia
Leishmaniose
Bioquímica sérica
Urina
topic Histopathology
Leishmaniasis
Serum biochemistry
Urine.
Agricultural Sciences
Histopatologia
Leishmaniose
Bioquímica sérica
Urina
description The visceral establishment of Leishmania infantum in dogs may result in kidney and bladder tissue injury, with L. infantum ending up in urine. This study therefore aimed at investigating the presence of Leishmania sp. in urinary sediments, and correlating the results with those from renal and bladder serum biochemistry and histopathology. Thirty dogs with negative Nested-Polymerase Chain Reaction (PCR) for E. canis were used in the experiment, and were divided into three groups: control group (10 dogs), neither leishmaniasis nor clinical changes; group I (15 dogs), leishmaniasis but no Leishmania sp. in urine; and group II (5 dogs), leishmaniasis, as well as Leishmania sp. in urine. All animals were submitted to clinical, serological, and parasitological diagnosis for leishmaniasis, biochemical exams, and kidney and bladder histopathology. The parasite was also detected in the bladder imprint of one group II dog. Group II dogs presented with very low albumin concentrations, low albumin/globulin ratios, and kidney and bladder lesions. In the kidneys, hydropic degeneration, thickened Bowman's capsule, and thickening of the tubular capsule were detected in all dogs with positive urinary sediment. However, no significant difference in these renal changes was observed between groups. The intensity and distribution of bladder inflammatory infiltrates were significantly (p-value < 0.05, Kruskal-Wallis’ and Dunn’s tests) higher in group II dogs, compared with those of the other groups. The presence of Leishmania sp. in the urine of infected dogs appeared to be related to low serum albumin concentrations and more severe bladder lesions.
publishDate 2020
dc.date.none.fl_str_mv 2020-04-13
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.ufu.br/index.php/biosciencejournal/article/view/42464
10.14393/BJ-v36n3a2020-42464
url https://seer.ufu.br/index.php/biosciencejournal/article/view/42464
identifier_str_mv 10.14393/BJ-v36n3a2020-42464
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.ufu.br/index.php/biosciencejournal/article/view/42464/28647
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Brazil; Contemporary
dc.publisher.none.fl_str_mv EDUFU
publisher.none.fl_str_mv EDUFU
dc.source.none.fl_str_mv Bioscience Journal ; Vol. 36 No. 3 (2020): May/June; 956-967
Bioscience Journal ; v. 36 n. 3 (2020): Maio/Junho; 956-967
1981-3163
reponame:Bioscience journal (Online)
instname:Universidade Federal de Uberlândia (UFU)
instacron:UFU
instname_str Universidade Federal de Uberlândia (UFU)
instacron_str UFU
institution UFU
reponame_str Bioscience journal (Online)
collection Bioscience journal (Online)
repository.name.fl_str_mv Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)
repository.mail.fl_str_mv biosciencej@ufu.br||
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