Spray drying of cellulases produced by Bacillus sp. SMIA-2
Autor(a) principal: | |
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Data de Publicação: | 2024 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Bioscience journal (Online) |
Texto Completo: | https://seer.ufu.br/index.php/biosciencejournal/article/view/64855 |
Resumo: | Microbial cellulases present biotechnological potential to be used in several industries, including food, brewery and wine, industrial waste for chemical feedstock, animal feed, pulp and paper, agriculture, textile and detergent production. In this work, cellulases produced by the thermophilic Bacillus sp. SMIA-2 in submerged cultures containing sugarcane bagasse, corn steep liquor and passion fruit rind flour were spray-dried, in an attempt to improve their stability for industrial purposes. The cellulases were spray dried and analyzed before and after the drying process and subsequent storage. A Central Composite Design (CCD) 23 was used to investigate the effect of different concentrations of arabic gum and microcrystalline cellulose, as well as the spray dryer inlet temperature on the cellulase spray drying process. The results evidenced that the combination of 1.0 % (w/v) arabic gum and 1.0% (w/v) microcrystalline cellulose, at inlet temperature of 70 °C, was effective in maintaining the activities of both avicelases (avicel-hydrolyzing enzymes) and carboxymethylcellulases (carboxymethylcellulose-hydrolyzing enzymes - CMCase). The dried avicelase was completely preserved when stored at 5oC, while the CMCase retained 89% of its activity, which indicates promising potential for industrial uses, especially in detergent formulations. |
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Spray drying of cellulases produced by Bacillus sp. SMIA-2AdditivesAvicelaseBacillusCarboxymethylcellulaseSpray drying.Biological SciencesMicrobial cellulases present biotechnological potential to be used in several industries, including food, brewery and wine, industrial waste for chemical feedstock, animal feed, pulp and paper, agriculture, textile and detergent production. In this work, cellulases produced by the thermophilic Bacillus sp. SMIA-2 in submerged cultures containing sugarcane bagasse, corn steep liquor and passion fruit rind flour were spray-dried, in an attempt to improve their stability for industrial purposes. The cellulases were spray dried and analyzed before and after the drying process and subsequent storage. A Central Composite Design (CCD) 23 was used to investigate the effect of different concentrations of arabic gum and microcrystalline cellulose, as well as the spray dryer inlet temperature on the cellulase spray drying process. The results evidenced that the combination of 1.0 % (w/v) arabic gum and 1.0% (w/v) microcrystalline cellulose, at inlet temperature of 70 °C, was effective in maintaining the activities of both avicelases (avicel-hydrolyzing enzymes) and carboxymethylcellulases (carboxymethylcellulose-hydrolyzing enzymes - CMCase). The dried avicelase was completely preserved when stored at 5oC, while the CMCase retained 89% of its activity, which indicates promising potential for industrial uses, especially in detergent formulations.Universidade Federal de Uberlândia2024-03-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/6485510.14393/BJ-v40n0a2024-64855Bioscience Journal ; Vol. 40 (2024): Continuous Publication; e40018Bioscience Journal ; v. 40 (2024): Continuous Publication; e400181981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/64855/38422Brazil; Contemporary Copyright (c) 2024 Erica Cruz, Simone Vilela Talma, João Batista Barbosa, Raphael Pires Bolzan, Silvania Alves Ladeira, Raquel Vieira de Carvalho, Meire Lelis Leal Martinshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccess Cruz, EricaTalma, Simone VilelaBarbosa, João BatistaPires Bolzan, RaphaelLadeira, Silvania AlvesVieira de Carvalho, RaquelLelis Leal Martins, Meire2024-04-03T12:44:09Zoai:ojs.www.seer.ufu.br:article/64855Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2024-04-03T12:44:09Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false |
dc.title.none.fl_str_mv |
Spray drying of cellulases produced by Bacillus sp. SMIA-2 |
title |
Spray drying of cellulases produced by Bacillus sp. SMIA-2 |
spellingShingle |
Spray drying of cellulases produced by Bacillus sp. SMIA-2 Cruz, Erica Additives Avicelase Bacillus Carboxymethylcellulase Spray drying. Biological Sciences |
title_short |
Spray drying of cellulases produced by Bacillus sp. SMIA-2 |
title_full |
Spray drying of cellulases produced by Bacillus sp. SMIA-2 |
title_fullStr |
Spray drying of cellulases produced by Bacillus sp. SMIA-2 |
title_full_unstemmed |
Spray drying of cellulases produced by Bacillus sp. SMIA-2 |
title_sort |
Spray drying of cellulases produced by Bacillus sp. SMIA-2 |
author |
Cruz, Erica |
author_facet |
Cruz, Erica Talma, Simone Vilela Barbosa, João Batista Pires Bolzan, Raphael Ladeira, Silvania Alves Vieira de Carvalho, Raquel Lelis Leal Martins, Meire |
author_role |
author |
author2 |
Talma, Simone Vilela Barbosa, João Batista Pires Bolzan, Raphael Ladeira, Silvania Alves Vieira de Carvalho, Raquel Lelis Leal Martins, Meire |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Cruz, Erica Talma, Simone Vilela Barbosa, João Batista Pires Bolzan, Raphael Ladeira, Silvania Alves Vieira de Carvalho, Raquel Lelis Leal Martins, Meire |
dc.subject.por.fl_str_mv |
Additives Avicelase Bacillus Carboxymethylcellulase Spray drying. Biological Sciences |
topic |
Additives Avicelase Bacillus Carboxymethylcellulase Spray drying. Biological Sciences |
description |
Microbial cellulases present biotechnological potential to be used in several industries, including food, brewery and wine, industrial waste for chemical feedstock, animal feed, pulp and paper, agriculture, textile and detergent production. In this work, cellulases produced by the thermophilic Bacillus sp. SMIA-2 in submerged cultures containing sugarcane bagasse, corn steep liquor and passion fruit rind flour were spray-dried, in an attempt to improve their stability for industrial purposes. The cellulases were spray dried and analyzed before and after the drying process and subsequent storage. A Central Composite Design (CCD) 23 was used to investigate the effect of different concentrations of arabic gum and microcrystalline cellulose, as well as the spray dryer inlet temperature on the cellulase spray drying process. The results evidenced that the combination of 1.0 % (w/v) arabic gum and 1.0% (w/v) microcrystalline cellulose, at inlet temperature of 70 °C, was effective in maintaining the activities of both avicelases (avicel-hydrolyzing enzymes) and carboxymethylcellulases (carboxymethylcellulose-hydrolyzing enzymes - CMCase). The dried avicelase was completely preserved when stored at 5oC, while the CMCase retained 89% of its activity, which indicates promising potential for industrial uses, especially in detergent formulations. |
publishDate |
2024 |
dc.date.none.fl_str_mv |
2024-03-15 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/64855 10.14393/BJ-v40n0a2024-64855 |
url |
https://seer.ufu.br/index.php/biosciencejournal/article/view/64855 |
identifier_str_mv |
10.14393/BJ-v40n0a2024-64855 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/64855/38422 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
Brazil; Contemporary |
dc.publisher.none.fl_str_mv |
Universidade Federal de Uberlândia |
publisher.none.fl_str_mv |
Universidade Federal de Uberlândia |
dc.source.none.fl_str_mv |
Bioscience Journal ; Vol. 40 (2024): Continuous Publication; e40018 Bioscience Journal ; v. 40 (2024): Continuous Publication; e40018 1981-3163 reponame:Bioscience journal (Online) instname:Universidade Federal de Uberlândia (UFU) instacron:UFU |
instname_str |
Universidade Federal de Uberlândia (UFU) |
instacron_str |
UFU |
institution |
UFU |
reponame_str |
Bioscience journal (Online) |
collection |
Bioscience journal (Online) |
repository.name.fl_str_mv |
Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU) |
repository.mail.fl_str_mv |
biosciencej@ufu.br|| |
_version_ |
1797069065239396352 |