Rouleaux formation of white blood cells and platelets in leukemia

Detalhes bibliográficos
Autor(a) principal: Ullah, Hafeez
Data de Publicação: 2018
Outros Autores: Naeem, Khalid, Akhtar, Munir, Hussain, Fayyaz, Ahmad, Mukhtar
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Bioscience journal (Online)
Texto Completo: https://seer.ufu.br/index.php/biosciencejournal/article/view/36976
Resumo: The objective of this study was to measure the effects of glucose and salt level on white blood cells, red blood cells and platelets (PLTs) in the blood of a leukemic patient by using a white light microscope. Different concentrations of glucose and salt in the range of 0 mM to 500 mM were admixed in the blood sample to prepare blood smear. We revealed that shape of erythrocytes, leukocytes and platelets changes and form aggregates. Increasing concentrations of glucose cause to increases aggregation process of white blood cells, red blood cells and platelets. And the increasing concentration of sodium chloride causes to increase rouleaux formation and aggregation of platelets but dehydration due to increased sodium chloride concentration causes to break the aggregation of white blood cells. Comparison of CBC reports of these samples with and without analytes shows that total leukocyte count (TLC) decreases gradually towards normal ranges of leukocytes which is favorable in the treatment of leukemia but at the same time decreasing level of hemoglobin HGB, mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC) and increasing level of red blood cell (RBCs) causes to reduce oxygen supply which is in favor of cancer growth and anemia. This work provides us the base for translation this in vitro study towards the in vivo case of blood microvasculature as a non-invasive methodology.
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spelling Rouleaux formation of white blood cells and platelets in leukemia Formação de rouleaux de glóbulos brancos e plaquetas em leucemiaWhite light microscopyComplete blood count (CBC)Total leukocyte count (TLC)Red blood cell (RBC)White blood cell (WBC)Mean corpuscular hemoglobin (MCH)Mean corpuscular hemoglobin concentration (MCHC)Biological SciencesThe objective of this study was to measure the effects of glucose and salt level on white blood cells, red blood cells and platelets (PLTs) in the blood of a leukemic patient by using a white light microscope. Different concentrations of glucose and salt in the range of 0 mM to 500 mM were admixed in the blood sample to prepare blood smear. We revealed that shape of erythrocytes, leukocytes and platelets changes and form aggregates. Increasing concentrations of glucose cause to increases aggregation process of white blood cells, red blood cells and platelets. And the increasing concentration of sodium chloride causes to increase rouleaux formation and aggregation of platelets but dehydration due to increased sodium chloride concentration causes to break the aggregation of white blood cells. Comparison of CBC reports of these samples with and without analytes shows that total leukocyte count (TLC) decreases gradually towards normal ranges of leukocytes which is favorable in the treatment of leukemia but at the same time decreasing level of hemoglobin HGB, mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC) and increasing level of red blood cell (RBCs) causes to reduce oxygen supply which is in favor of cancer growth and anemia. This work provides us the base for translation this in vitro study towards the in vivo case of blood microvasculature as a non-invasive methodology.O objetivo deste estudo foi medir os efeitos da glicose e do nível de sal nos glóbulos brancos, glóbulos vermelhos e plaquetas (PLTs) no sangue de um paciente leucêmico usando um microscópio de luz branca. Foram misturadas diferentes concentrações de glicose e sal na gama de 0 mM a 500 mM na amostra de sangue para preparar esfregaço de sangue. Descrevemos que a forma dos eritrócitos, leucócitos e plaquetas muda e forma agregados. O aumento das concentrações de glicose aumenta o processo de agregação de glóbulos brancos, glóbulos vermelhos e plaquetas. E a crescente concentração de cloreto de sódio causa o aumento da formação de rouleaux e a agregação de plaquetas, mas a desidratação devido ao aumento da concentração de cloreto de sódio causa a quebra da agregação de glóbulos brancos. A comparação dos relatórios de CBC dessas amostras com e sem analitos mostra que a contagem total de leucócitos (TLC)diminui gradualmente para os intervalos normais de leucócitos, o que é favorável no tratamento da leucemia, mas ao mesmo tempo diminui o nível de hemoglobina HGB, hemoglobina corpuscular média (MCH ), a concentração média de hemoglobina corpuscular (MCHC) e o aumento do nível de glóbulos vermelhos (RBCs) reduz o suprimento de oxigênio, o que é a favor do crescimento do câncer e da anemia. Este trabalho fornece a base para a tradução deste estudo in vitro para o caso in vivo de microvasculatura de sangue como uma metodologia não-invasiva. EDUFU2018-03-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/3697610.14393/BJ-v34n2a2018-36976Bioscience Journal ; Vol. 34 No. 2 (2018): Mar./Apr.; 410-422Bioscience Journal ; v. 34 n. 2 (2018): Mar./Apr.; 410-4221981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/36976/21921Brazil; ContemporaryCopyright (c) 2018 Hafeez Ullah, Khalid Naeem, Munir Akhtar, Fayyaz Hussain, Mukhtar Ahmadhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessUllah, HafeezNaeem, KhalidAkhtar, MunirHussain, FayyazAhmad, Mukhtar2022-02-15T18:20:03Zoai:ojs.www.seer.ufu.br:article/36976Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2022-02-15T18:20:03Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false
dc.title.none.fl_str_mv Rouleaux formation of white blood cells and platelets in leukemia
Formação de rouleaux de glóbulos brancos e plaquetas em leucemia
title Rouleaux formation of white blood cells and platelets in leukemia
spellingShingle Rouleaux formation of white blood cells and platelets in leukemia
Ullah, Hafeez
White light microscopy
Complete blood count (CBC)
Total leukocyte count (TLC)
Red blood cell (RBC)
White blood cell (WBC)
Mean corpuscular hemoglobin (MCH)
Mean corpuscular hemoglobin concentration (MCHC)
Biological Sciences
title_short Rouleaux formation of white blood cells and platelets in leukemia
title_full Rouleaux formation of white blood cells and platelets in leukemia
title_fullStr Rouleaux formation of white blood cells and platelets in leukemia
title_full_unstemmed Rouleaux formation of white blood cells and platelets in leukemia
title_sort Rouleaux formation of white blood cells and platelets in leukemia
author Ullah, Hafeez
author_facet Ullah, Hafeez
Naeem, Khalid
Akhtar, Munir
Hussain, Fayyaz
Ahmad, Mukhtar
author_role author
author2 Naeem, Khalid
Akhtar, Munir
Hussain, Fayyaz
Ahmad, Mukhtar
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Ullah, Hafeez
Naeem, Khalid
Akhtar, Munir
Hussain, Fayyaz
Ahmad, Mukhtar
dc.subject.por.fl_str_mv White light microscopy
Complete blood count (CBC)
Total leukocyte count (TLC)
Red blood cell (RBC)
White blood cell (WBC)
Mean corpuscular hemoglobin (MCH)
Mean corpuscular hemoglobin concentration (MCHC)
Biological Sciences
topic White light microscopy
Complete blood count (CBC)
Total leukocyte count (TLC)
Red blood cell (RBC)
White blood cell (WBC)
Mean corpuscular hemoglobin (MCH)
Mean corpuscular hemoglobin concentration (MCHC)
Biological Sciences
description The objective of this study was to measure the effects of glucose and salt level on white blood cells, red blood cells and platelets (PLTs) in the blood of a leukemic patient by using a white light microscope. Different concentrations of glucose and salt in the range of 0 mM to 500 mM were admixed in the blood sample to prepare blood smear. We revealed that shape of erythrocytes, leukocytes and platelets changes and form aggregates. Increasing concentrations of glucose cause to increases aggregation process of white blood cells, red blood cells and platelets. And the increasing concentration of sodium chloride causes to increase rouleaux formation and aggregation of platelets but dehydration due to increased sodium chloride concentration causes to break the aggregation of white blood cells. Comparison of CBC reports of these samples with and without analytes shows that total leukocyte count (TLC) decreases gradually towards normal ranges of leukocytes which is favorable in the treatment of leukemia but at the same time decreasing level of hemoglobin HGB, mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC) and increasing level of red blood cell (RBCs) causes to reduce oxygen supply which is in favor of cancer growth and anemia. This work provides us the base for translation this in vitro study towards the in vivo case of blood microvasculature as a non-invasive methodology.
publishDate 2018
dc.date.none.fl_str_mv 2018-03-26
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.ufu.br/index.php/biosciencejournal/article/view/36976
10.14393/BJ-v34n2a2018-36976
url https://seer.ufu.br/index.php/biosciencejournal/article/view/36976
identifier_str_mv 10.14393/BJ-v34n2a2018-36976
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.ufu.br/index.php/biosciencejournal/article/view/36976/21921
dc.rights.driver.fl_str_mv Copyright (c) 2018 Hafeez Ullah, Khalid Naeem, Munir Akhtar, Fayyaz Hussain, Mukhtar Ahmad
https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Hafeez Ullah, Khalid Naeem, Munir Akhtar, Fayyaz Hussain, Mukhtar Ahmad
https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Brazil; Contemporary
dc.publisher.none.fl_str_mv EDUFU
publisher.none.fl_str_mv EDUFU
dc.source.none.fl_str_mv Bioscience Journal ; Vol. 34 No. 2 (2018): Mar./Apr.; 410-422
Bioscience Journal ; v. 34 n. 2 (2018): Mar./Apr.; 410-422
1981-3163
reponame:Bioscience journal (Online)
instname:Universidade Federal de Uberlândia (UFU)
instacron:UFU
instname_str Universidade Federal de Uberlândia (UFU)
instacron_str UFU
institution UFU
reponame_str Bioscience journal (Online)
collection Bioscience journal (Online)
repository.name.fl_str_mv Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)
repository.mail.fl_str_mv biosciencej@ufu.br||
_version_ 1797069077705916416

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