In vitro conservation and leaf anatomy of different chemotypes of Lippia alba (Mill.) N. E. BR
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Bioscience journal (Online) |
Texto Completo: | https://seer.ufu.br/index.php/biosciencejournal/article/view/35757 |
Resumo: | The aim of this work was to develop an in vitro conservation protocol for Lippia alba that involved mineral oil and to study the leaf anatomy of L. alba genotypes in in vitro and ex vitro environments. In vitro conservation involved five genotypes; LA-13 and LA-57 (carvone chemotype); LA-22 (linalool chemotype); and LA-29 and LA-44 (citral chemotype). Each genotype was treated with mineral oil, with water, and a control at 18°C and 23°C. Survival (%), shoot height (cm) and leaf color were assessed at 180 and 270 days. The genotypes with the best in vitro conservation outcomes (LA-13 and LA-57) were used to study the anatomy of the leaves. Midrib thickness, leaf blade thickness, adaxial and abaxial cuticles and the number of glandular and tector trichomes were assessed. A temperature of 18°C was optimal for the conservation of genotypes of the carvone chemotypes. The explants were short and had green leaves. This was especially true for LA-57, whose height did not exceed 2.0 cm. At 270 days, the LA-13 and LA-57 genotypes continued to have green and viable leaves, especially LA-57, which had the lowest mean height. Field plants of the carvone chemotype had thicker midribs, leaf blades, and cuticles and had more trichomes. The LA-57 genotype had the most glandular and tector trichomes. |
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In vitro conservation and leaf anatomy of different chemotypes of Lippia alba (Mill.) N. E. BR Conservação in vitro e anatomia foliar de diferentes quimiotipios de Lippia alba (Mill.) N. E. BRVerbenaceaeSlow GrowthPlant anatomyMorphologyAgricultural SciencesThe aim of this work was to develop an in vitro conservation protocol for Lippia alba that involved mineral oil and to study the leaf anatomy of L. alba genotypes in in vitro and ex vitro environments. In vitro conservation involved five genotypes; LA-13 and LA-57 (carvone chemotype); LA-22 (linalool chemotype); and LA-29 and LA-44 (citral chemotype). Each genotype was treated with mineral oil, with water, and a control at 18°C and 23°C. Survival (%), shoot height (cm) and leaf color were assessed at 180 and 270 days. The genotypes with the best in vitro conservation outcomes (LA-13 and LA-57) were used to study the anatomy of the leaves. Midrib thickness, leaf blade thickness, adaxial and abaxial cuticles and the number of glandular and tector trichomes were assessed. A temperature of 18°C was optimal for the conservation of genotypes of the carvone chemotypes. The explants were short and had green leaves. This was especially true for LA-57, whose height did not exceed 2.0 cm. At 270 days, the LA-13 and LA-57 genotypes continued to have green and viable leaves, especially LA-57, which had the lowest mean height. Field plants of the carvone chemotype had thicker midribs, leaf blades, and cuticles and had more trichomes. The LA-57 genotype had the most glandular and tector trichomes. O objetivo deste estudo foi desenvolver um protocolo de conservação in vitro com óleo mineral e estudar a anatomia foliar de genótipos de L. alba nos ambientes in vitro e ex vitro. A conservação in vitro constou de cinco genótipos LA-13 e LA-57 (quimiotipo carvona); LA-22 (quimiotipo linalol); LA-29 e LA-44 (quimiotipo citral), três tratamentos (com óleo mineral, com água e controle) e duas temperaturas 18 e 23°C. Aos 180 e 270 dias, avaliou-se a sobrevivência (%), altura da parte aérea (cm) e coloração das folhas. Os genótipos com os melhores resultados da conservação in vitro (LA-13 e LA-57) foram utilizados para estudos de anatomia. Avaliou-se espessura da nervura central, espessura do limbo foliar, cutículas adaxial e abaxial e quantidade de tricomas glandulares e tectores. A temperatura a 18°C foi a ideal para a conservação dos genótipos do quimiotipo carvona, com folhas verdes e baixos valores para altura, destacando-se LA-57 cuja altura não ultrapassou 2,0 cm. Aos 270 dias LA-13 e LA-57 permaneceram com folhas verdes e viáveis, com destaque para LA-57 com menores médias de altura. A anatomia do quimiotipo carvona mostrou que as plantas de campo apresentaram maiores espessuras da nervura, limbo e cutículas, e maiores quantidades de tricomas. O genótipo LA-57 obteve a maior quantidade de tricomas glandulares e tectores.EDUFU2017-02-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/3575710.14393/BJ-v33n1a2017-35757Bioscience Journal ; Vol. 33 No. 1 (2017): Jan./Feb.; 41-51Bioscience Journal ; v. 33 n. 1 (2017): Jan./Feb.; 41-511981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/35757/19748Brazil; ContemporaryCopyright (c) 2017 Magna Galvão Peixoto, Ana Catarina Lima de Oliveira, Arie Fitzgerald Blank, Jefferson Henrique Santos Silva, José Magno Queiroz Luz, Maria de Fátima Arrigoni-Blankhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessPeixoto, Magna GalvãoOliveira, Ana Catarina Lima deBlank, Arie FitzgeraldSilva, Jefferson Henrique SantosLuz, José Magno QueirozArrigoni-Blank, Maria de Fátima2022-02-16T12:03:45Zoai:ojs.www.seer.ufu.br:article/35757Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2022-02-16T12:03:45Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false |
dc.title.none.fl_str_mv |
In vitro conservation and leaf anatomy of different chemotypes of Lippia alba (Mill.) N. E. BR Conservação in vitro e anatomia foliar de diferentes quimiotipios de Lippia alba (Mill.) N. E. BR |
title |
In vitro conservation and leaf anatomy of different chemotypes of Lippia alba (Mill.) N. E. BR |
spellingShingle |
In vitro conservation and leaf anatomy of different chemotypes of Lippia alba (Mill.) N. E. BR Peixoto, Magna Galvão Verbenaceae Slow Growth Plant anatomy Morphology Agricultural Sciences |
title_short |
In vitro conservation and leaf anatomy of different chemotypes of Lippia alba (Mill.) N. E. BR |
title_full |
In vitro conservation and leaf anatomy of different chemotypes of Lippia alba (Mill.) N. E. BR |
title_fullStr |
In vitro conservation and leaf anatomy of different chemotypes of Lippia alba (Mill.) N. E. BR |
title_full_unstemmed |
In vitro conservation and leaf anatomy of different chemotypes of Lippia alba (Mill.) N. E. BR |
title_sort |
In vitro conservation and leaf anatomy of different chemotypes of Lippia alba (Mill.) N. E. BR |
author |
Peixoto, Magna Galvão |
author_facet |
Peixoto, Magna Galvão Oliveira, Ana Catarina Lima de Blank, Arie Fitzgerald Silva, Jefferson Henrique Santos Luz, José Magno Queiroz Arrigoni-Blank, Maria de Fátima |
author_role |
author |
author2 |
Oliveira, Ana Catarina Lima de Blank, Arie Fitzgerald Silva, Jefferson Henrique Santos Luz, José Magno Queiroz Arrigoni-Blank, Maria de Fátima |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Peixoto, Magna Galvão Oliveira, Ana Catarina Lima de Blank, Arie Fitzgerald Silva, Jefferson Henrique Santos Luz, José Magno Queiroz Arrigoni-Blank, Maria de Fátima |
dc.subject.por.fl_str_mv |
Verbenaceae Slow Growth Plant anatomy Morphology Agricultural Sciences |
topic |
Verbenaceae Slow Growth Plant anatomy Morphology Agricultural Sciences |
description |
The aim of this work was to develop an in vitro conservation protocol for Lippia alba that involved mineral oil and to study the leaf anatomy of L. alba genotypes in in vitro and ex vitro environments. In vitro conservation involved five genotypes; LA-13 and LA-57 (carvone chemotype); LA-22 (linalool chemotype); and LA-29 and LA-44 (citral chemotype). Each genotype was treated with mineral oil, with water, and a control at 18°C and 23°C. Survival (%), shoot height (cm) and leaf color were assessed at 180 and 270 days. The genotypes with the best in vitro conservation outcomes (LA-13 and LA-57) were used to study the anatomy of the leaves. Midrib thickness, leaf blade thickness, adaxial and abaxial cuticles and the number of glandular and tector trichomes were assessed. A temperature of 18°C was optimal for the conservation of genotypes of the carvone chemotypes. The explants were short and had green leaves. This was especially true for LA-57, whose height did not exceed 2.0 cm. At 270 days, the LA-13 and LA-57 genotypes continued to have green and viable leaves, especially LA-57, which had the lowest mean height. Field plants of the carvone chemotype had thicker midribs, leaf blades, and cuticles and had more trichomes. The LA-57 genotype had the most glandular and tector trichomes. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-02-09 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/35757 10.14393/BJ-v33n1a2017-35757 |
url |
https://seer.ufu.br/index.php/biosciencejournal/article/view/35757 |
identifier_str_mv |
10.14393/BJ-v33n1a2017-35757 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/35757/19748 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
Brazil; Contemporary |
dc.publisher.none.fl_str_mv |
EDUFU |
publisher.none.fl_str_mv |
EDUFU |
dc.source.none.fl_str_mv |
Bioscience Journal ; Vol. 33 No. 1 (2017): Jan./Feb.; 41-51 Bioscience Journal ; v. 33 n. 1 (2017): Jan./Feb.; 41-51 1981-3163 reponame:Bioscience journal (Online) instname:Universidade Federal de Uberlândia (UFU) instacron:UFU |
instname_str |
Universidade Federal de Uberlândia (UFU) |
instacron_str |
UFU |
institution |
UFU |
reponame_str |
Bioscience journal (Online) |
collection |
Bioscience journal (Online) |
repository.name.fl_str_mv |
Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU) |
repository.mail.fl_str_mv |
biosciencej@ufu.br|| |
_version_ |
1797069065939845120 |