Antimicrobial effect against oral bacteria on bioactive compounds in a high-pressure enzymatic Prunus mume extract
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Bioscience journal (Online) |
Texto Completo: | https://seer.ufu.br/index.php/biosciencejournal/article/view/41417 |
Resumo: | To evaluate high-pressure processing combined with enzymatic treatment for extraction of Prunus mume by determining the optimum extraction conditions for the antimicrobial activity against oral bacteria. High-pressure enzymatic extraction was used to isolate biologically active components from P. mume. The effects of process variables such as enzyme type (Pectinex Ultra SP-L, Novozym 33095 and Viscozyme L), enzyme concentration, incubation time/temperature, pH, and ratio of enzymes antimicrobial activity against oral pathogens related to dental caries and periodontal diseases were determined by disk diffusion assay. The optimal conditions for enzymatic extraction from P. mume were pH 6.0, 45°C, 20 h, and 5 v/v% with Pectinex Ultra SP-L. The maximum antimicrobial activity of P. mume extract obtained using Novozym 33095 was at pH 7.0, 45°C, 20 h, and 5 v/v%. The Viscozyme L extract showed the maximum inhibitory effect at pH 6.0, 45°C, 20 h, and 5 v/v%. Use of combinations of enzymes did not result in significantly different antimicrobial activity (p < 0.05) compared with each enzyme alone. Minimum inhibitory concentration values were 3.125 to 12.50%. These results indicated that high-pressure enzymatic extraction yielded P. mume extract with antimicrobial activity which has the potential for improving oral environment. |
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Antimicrobial effect against oral bacteria on bioactive compounds in a high-pressure enzymatic Prunus mume extract Efeito antimicrobiano contra bactérias orais sobre compostos bioativos em um extrato enzimático de Prunus mume de alta pressão Antimicrobial activityHigh-pressure enzymatic extractMinimum inhibitory concentrationPrunus mumeHealth SciencesTo evaluate high-pressure processing combined with enzymatic treatment for extraction of Prunus mume by determining the optimum extraction conditions for the antimicrobial activity against oral bacteria. High-pressure enzymatic extraction was used to isolate biologically active components from P. mume. The effects of process variables such as enzyme type (Pectinex Ultra SP-L, Novozym 33095 and Viscozyme L), enzyme concentration, incubation time/temperature, pH, and ratio of enzymes antimicrobial activity against oral pathogens related to dental caries and periodontal diseases were determined by disk diffusion assay. The optimal conditions for enzymatic extraction from P. mume were pH 6.0, 45°C, 20 h, and 5 v/v% with Pectinex Ultra SP-L. The maximum antimicrobial activity of P. mume extract obtained using Novozym 33095 was at pH 7.0, 45°C, 20 h, and 5 v/v%. The Viscozyme L extract showed the maximum inhibitory effect at pH 6.0, 45°C, 20 h, and 5 v/v%. Use of combinations of enzymes did not result in significantly different antimicrobial activity (p < 0.05) compared with each enzyme alone. Minimum inhibitory concentration values were 3.125 to 12.50%. These results indicated that high-pressure enzymatic extraction yielded P. mume extract with antimicrobial activity which has the potential for improving oral environment.Avaliar o processamento de alta pressão combinado com o tratamento enzimático para a extração de Prunus mume, determinando as condições ótimas de extração para a atividade antimicrobiana contra as bactérias orais. A extração enzimática de alta pressão foi utilizada para isolar os componentes biologicamente ativos de P. mume. Os efeitos das variáveis do processo como o tipo enzimático (Pectinex Ultra SP-L, Novozym 33095 e Viscozyme L), a concentração de enzima, o tempo/temperatura de incubação, pH, e a relação da atividade antimicrobiana de enzimas contra os patógenos orais relacionados à cárie dentária e doenças periodontais foram determinados pelo ensaio de difusão em disco. As condições ótimas para a extração enzimática de P. mume foram pH 6.0, 45°C, 20 h, e 5 v/v% com Pectinex Ultra SP-L. A máxima atividade antimicrobiana do extrato de P. mume obtida usando Novozym 33095 foi em pH 7.0, 45°C, 20 h, e 5 v/v%. O extrato de Viscozyme L apresentou o efeito inibitório máximo em pH 6.0, 45°C, 20 h, e 5 v/v%. O uso de combinações de enzimas não resultou em uma atividade antimicrobiana significativamente diferente (p < 0.05) em comparação com cada enzima por separada. Os valores mínimos da concentração inibitória foram de 3.125 a 12.50%. Estes resultados indicaram que a extração enzimática de alta pressão produziu o extrato de P. mume com atividade antimicrobiana,o qual tem o potencial para melhorar o ambiente bucal.EDUFU2018-08-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/4141710.14393/BJ-v34n1a2018-41417Bioscience Journal ; Vol. 34 No. 4 (2018): July/Aug.; 1124-1136Bioscience Journal ; v. 34 n. 4 (2018): July/Aug.; 1124-11361981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/41417/22682Brazil; ContemporaryCopyright (c) 2018 Sang-Cheol Yu, So-Ra Han, Jong Hwa Jang, Yong-Duk Park, Tae-Jin Ohhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessYu, Sang-CheolHan, So-RaJang, Jong HwaPark, Yong-DukOh, Tae-Jin2022-02-14T13:19:59Zoai:ojs.www.seer.ufu.br:article/41417Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2022-02-14T13:19:59Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false |
dc.title.none.fl_str_mv |
Antimicrobial effect against oral bacteria on bioactive compounds in a high-pressure enzymatic Prunus mume extract Efeito antimicrobiano contra bactérias orais sobre compostos bioativos em um extrato enzimático de Prunus mume de alta pressão |
title |
Antimicrobial effect against oral bacteria on bioactive compounds in a high-pressure enzymatic Prunus mume extract |
spellingShingle |
Antimicrobial effect against oral bacteria on bioactive compounds in a high-pressure enzymatic Prunus mume extract Yu, Sang-Cheol Antimicrobial activity High-pressure enzymatic extract Minimum inhibitory concentration Prunus mume Health Sciences |
title_short |
Antimicrobial effect against oral bacteria on bioactive compounds in a high-pressure enzymatic Prunus mume extract |
title_full |
Antimicrobial effect against oral bacteria on bioactive compounds in a high-pressure enzymatic Prunus mume extract |
title_fullStr |
Antimicrobial effect against oral bacteria on bioactive compounds in a high-pressure enzymatic Prunus mume extract |
title_full_unstemmed |
Antimicrobial effect against oral bacteria on bioactive compounds in a high-pressure enzymatic Prunus mume extract |
title_sort |
Antimicrobial effect against oral bacteria on bioactive compounds in a high-pressure enzymatic Prunus mume extract |
author |
Yu, Sang-Cheol |
author_facet |
Yu, Sang-Cheol Han, So-Ra Jang, Jong Hwa Park, Yong-Duk Oh, Tae-Jin |
author_role |
author |
author2 |
Han, So-Ra Jang, Jong Hwa Park, Yong-Duk Oh, Tae-Jin |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Yu, Sang-Cheol Han, So-Ra Jang, Jong Hwa Park, Yong-Duk Oh, Tae-Jin |
dc.subject.por.fl_str_mv |
Antimicrobial activity High-pressure enzymatic extract Minimum inhibitory concentration Prunus mume Health Sciences |
topic |
Antimicrobial activity High-pressure enzymatic extract Minimum inhibitory concentration Prunus mume Health Sciences |
description |
To evaluate high-pressure processing combined with enzymatic treatment for extraction of Prunus mume by determining the optimum extraction conditions for the antimicrobial activity against oral bacteria. High-pressure enzymatic extraction was used to isolate biologically active components from P. mume. The effects of process variables such as enzyme type (Pectinex Ultra SP-L, Novozym 33095 and Viscozyme L), enzyme concentration, incubation time/temperature, pH, and ratio of enzymes antimicrobial activity against oral pathogens related to dental caries and periodontal diseases were determined by disk diffusion assay. The optimal conditions for enzymatic extraction from P. mume were pH 6.0, 45°C, 20 h, and 5 v/v% with Pectinex Ultra SP-L. The maximum antimicrobial activity of P. mume extract obtained using Novozym 33095 was at pH 7.0, 45°C, 20 h, and 5 v/v%. The Viscozyme L extract showed the maximum inhibitory effect at pH 6.0, 45°C, 20 h, and 5 v/v%. Use of combinations of enzymes did not result in significantly different antimicrobial activity (p < 0.05) compared with each enzyme alone. Minimum inhibitory concentration values were 3.125 to 12.50%. These results indicated that high-pressure enzymatic extraction yielded P. mume extract with antimicrobial activity which has the potential for improving oral environment. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-08-08 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/41417 10.14393/BJ-v34n1a2018-41417 |
url |
https://seer.ufu.br/index.php/biosciencejournal/article/view/41417 |
identifier_str_mv |
10.14393/BJ-v34n1a2018-41417 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/41417/22682 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Sang-Cheol Yu, So-Ra Han, Jong Hwa Jang, Yong-Duk Park, Tae-Jin Oh https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Sang-Cheol Yu, So-Ra Han, Jong Hwa Jang, Yong-Duk Park, Tae-Jin Oh https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
Brazil; Contemporary |
dc.publisher.none.fl_str_mv |
EDUFU |
publisher.none.fl_str_mv |
EDUFU |
dc.source.none.fl_str_mv |
Bioscience Journal ; Vol. 34 No. 4 (2018): July/Aug.; 1124-1136 Bioscience Journal ; v. 34 n. 4 (2018): July/Aug.; 1124-1136 1981-3163 reponame:Bioscience journal (Online) instname:Universidade Federal de Uberlândia (UFU) instacron:UFU |
instname_str |
Universidade Federal de Uberlândia (UFU) |
instacron_str |
UFU |
institution |
UFU |
reponame_str |
Bioscience journal (Online) |
collection |
Bioscience journal (Online) |
repository.name.fl_str_mv |
Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU) |
repository.mail.fl_str_mv |
biosciencej@ufu.br|| |
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1797069079150854144 |