Assessment of circulating MCP-1 level and 2518A>G gene polymorphism in systemic lupus erythematosus
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Bioscience journal (Online) |
Texto Completo: | https://seer.ufu.br/index.php/biosciencejournal/article/view/50922 |
Resumo: | Lupus nephritis (LN) is a major contributor to morbidity and mortality in patients with Systemic Lupus Erythematosus (SLE). This study aims to investigate the possible role of a functional polymorphism in the regulatory region of the monocyte chemo-attractant protein-1 (MCP-1) gene and MCP-1 blood level in the diagnosis of LN and in correlating the MCP-1 blood levels with disease activity. The study included 56 SLE patients and 56 controls. All the SLE patients suffered from LN. An analysis of MCP-1 gene polymorphism by polymerase chain reaction was performed followed by restriction fragment length polymorphism (PCR-RFLP) analysis and MCP-1 blood level was determined using the ELISA technique. Calculation of Systemic Lupus Erythematosus Disease Activity Index (SLEDAI) was performed. Serologic tests included the determination of antinuclear antibody (ANA) and anti-double-stranded (ds) DNA antibodies, Complement C3 and C4 levels. A significant increase in the frequency of genotype A/G and a decrease in the frequency of genotype A/A were found among patients with active LN compared to inactive LN. There was a statistically significant difference in the blood level of MCP-1 between LN patients and controls. Also, MCP-1 blood levels were significantly higher in active LN patients than inactive LN. A significant positive linear correlation was detected between MCP-1 blood level and SLEDAI, creatinine, and 24 hours protein in LN patients. These results suggest that an A/G genotype together with the measurement of the blood level of MCP-1 can be a useful tool for detection and follow up of active LN. |
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Assessment of circulating MCP-1 level and 2518A>G gene polymorphism in systemic lupus erythematosusAvaliação do nível circulante de MCP-1 e polimorfismo do gene 2518A>G no lúpus eritematoso sistêmicoSLE.LN.MCP-1.Gene polymorphism.Health SciencesLES.LN.MCP-1.Polimorfismo genético.Lupus nephritis (LN) is a major contributor to morbidity and mortality in patients with Systemic Lupus Erythematosus (SLE). This study aims to investigate the possible role of a functional polymorphism in the regulatory region of the monocyte chemo-attractant protein-1 (MCP-1) gene and MCP-1 blood level in the diagnosis of LN and in correlating the MCP-1 blood levels with disease activity. The study included 56 SLE patients and 56 controls. All the SLE patients suffered from LN. An analysis of MCP-1 gene polymorphism by polymerase chain reaction was performed followed by restriction fragment length polymorphism (PCR-RFLP) analysis and MCP-1 blood level was determined using the ELISA technique. Calculation of Systemic Lupus Erythematosus Disease Activity Index (SLEDAI) was performed. Serologic tests included the determination of antinuclear antibody (ANA) and anti-double-stranded (ds) DNA antibodies, Complement C3 and C4 levels. A significant increase in the frequency of genotype A/G and a decrease in the frequency of genotype A/A were found among patients with active LN compared to inactive LN. There was a statistically significant difference in the blood level of MCP-1 between LN patients and controls. Also, MCP-1 blood levels were significantly higher in active LN patients than inactive LN. A significant positive linear correlation was detected between MCP-1 blood level and SLEDAI, creatinine, and 24 hours protein in LN patients. These results suggest that an A/G genotype together with the measurement of the blood level of MCP-1 can be a useful tool for detection and follow up of active LN. A nefrite do lúpus (LN) é um dos principais contribuintes para a morbidade e mortalidade em pacientes com o Lúpus Eritematoso Sistémico (LES). Este estudo tem como objetivo investigar o possível papel de um polimorfismo funcional na região reguladora do gene da proteína quimioatraente de monócitos-1 (MCP-1) e do nível sanguíneo de MCP-1 no diagnóstico de LN e na correlação do sangue de MCP-1 níveis com atividade da doença. O estudo incluiu 56 pacientes com LES e 56 controles. Todos os pacientes com LES sofriam de LN. Uma análise do polimorfismo do gene MCP-1 por reação em cadeia da polimerase foi realizada seguida pela análise do polimorfismo do comprimento do fragmento de restrição (PCR-RFLP) e o nível sanguíneo do MCP-1 foi determinado pela técnica ELISA. O cálculo do índice de atividade da doença sistêmica do lúpus eritematoso (SLEDAI) foi realizado. Os testes sorológicos incluíram a determinação de anticorpos antinucleares (ANA) e anticorpos anti-DNA de fita dupla (ds), níveis de Complemento C3 e C4. Um aumento significativo na frequência do genótipo A/G e uma diminuição na frequência do genótipo A/A foram encontrados entre os pacientes com LN ativo em comparação com o LN inativo. Houve uma diferença estatisticamente significante no nível sanguíneo de MCP-1 entre pacientes com LN e controles. Além disso, os níveis sanguíneos de MCP-1 foram significativamente mais altos em pacientes com LN ativo do que com LN inativo. Uma correlação linear positiva significativa foi detectada entre o nível sanguíneo de MCP-1 e SLEDAI, creatinina e proteína de 24 horas em pacientes com LN. Esses resultados sugerem que um genótipo A/G, juntamente com a medição do nível sanguíneo de MCP-1, pode ser uma ferramenta útil para a detecção e acompanhamento do LN ativo.EDUFU2020-08-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/5092210.14393/BJ-v36n5a2020-50922Bioscience Journal ; Vol. 36 No. 5 (2020): Sept./Oct.; 1750-1759Bioscience Journal ; v. 36 n. 5 (2020): Sept./Oct.; 1750-17591981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/50922/29637Egypt; ContemporaryCopyright (c) 2020 Phebeebe Abdelmesseihbdel-Messih, Hussein S. El-Fishawy, Abeer Mohamed Ahmed Zahran, Noha Mahmoud Abdel Bakihttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessAbdelmesseihbdel-Messih, Phebeebe El-Fishawy, Hussein S.Zahran, Abeer Mohamed Ahmed Baki, Noha Mahmoud Abdel 2022-06-14T11:27:30Zoai:ojs.www.seer.ufu.br:article/50922Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2022-06-14T11:27:30Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false |
dc.title.none.fl_str_mv |
Assessment of circulating MCP-1 level and 2518A>G gene polymorphism in systemic lupus erythematosus Avaliação do nível circulante de MCP-1 e polimorfismo do gene 2518A>G no lúpus eritematoso sistêmico |
title |
Assessment of circulating MCP-1 level and 2518A>G gene polymorphism in systemic lupus erythematosus |
spellingShingle |
Assessment of circulating MCP-1 level and 2518A>G gene polymorphism in systemic lupus erythematosus Abdelmesseihbdel-Messih, Phebeebe SLE. LN. MCP-1. Gene polymorphism. Health Sciences LES. LN. MCP-1. Polimorfismo genético. |
title_short |
Assessment of circulating MCP-1 level and 2518A>G gene polymorphism in systemic lupus erythematosus |
title_full |
Assessment of circulating MCP-1 level and 2518A>G gene polymorphism in systemic lupus erythematosus |
title_fullStr |
Assessment of circulating MCP-1 level and 2518A>G gene polymorphism in systemic lupus erythematosus |
title_full_unstemmed |
Assessment of circulating MCP-1 level and 2518A>G gene polymorphism in systemic lupus erythematosus |
title_sort |
Assessment of circulating MCP-1 level and 2518A>G gene polymorphism in systemic lupus erythematosus |
author |
Abdelmesseihbdel-Messih, Phebeebe |
author_facet |
Abdelmesseihbdel-Messih, Phebeebe El-Fishawy, Hussein S. Zahran, Abeer Mohamed Ahmed Baki, Noha Mahmoud Abdel |
author_role |
author |
author2 |
El-Fishawy, Hussein S. Zahran, Abeer Mohamed Ahmed Baki, Noha Mahmoud Abdel |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Abdelmesseihbdel-Messih, Phebeebe El-Fishawy, Hussein S. Zahran, Abeer Mohamed Ahmed Baki, Noha Mahmoud Abdel |
dc.subject.por.fl_str_mv |
SLE. LN. MCP-1. Gene polymorphism. Health Sciences LES. LN. MCP-1. Polimorfismo genético. |
topic |
SLE. LN. MCP-1. Gene polymorphism. Health Sciences LES. LN. MCP-1. Polimorfismo genético. |
description |
Lupus nephritis (LN) is a major contributor to morbidity and mortality in patients with Systemic Lupus Erythematosus (SLE). This study aims to investigate the possible role of a functional polymorphism in the regulatory region of the monocyte chemo-attractant protein-1 (MCP-1) gene and MCP-1 blood level in the diagnosis of LN and in correlating the MCP-1 blood levels with disease activity. The study included 56 SLE patients and 56 controls. All the SLE patients suffered from LN. An analysis of MCP-1 gene polymorphism by polymerase chain reaction was performed followed by restriction fragment length polymorphism (PCR-RFLP) analysis and MCP-1 blood level was determined using the ELISA technique. Calculation of Systemic Lupus Erythematosus Disease Activity Index (SLEDAI) was performed. Serologic tests included the determination of antinuclear antibody (ANA) and anti-double-stranded (ds) DNA antibodies, Complement C3 and C4 levels. A significant increase in the frequency of genotype A/G and a decrease in the frequency of genotype A/A were found among patients with active LN compared to inactive LN. There was a statistically significant difference in the blood level of MCP-1 between LN patients and controls. Also, MCP-1 blood levels were significantly higher in active LN patients than inactive LN. A significant positive linear correlation was detected between MCP-1 blood level and SLEDAI, creatinine, and 24 hours protein in LN patients. These results suggest that an A/G genotype together with the measurement of the blood level of MCP-1 can be a useful tool for detection and follow up of active LN. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-08-13 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/50922 10.14393/BJ-v36n5a2020-50922 |
url |
https://seer.ufu.br/index.php/biosciencejournal/article/view/50922 |
identifier_str_mv |
10.14393/BJ-v36n5a2020-50922 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/50922/29637 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
Egypt; Contemporary |
dc.publisher.none.fl_str_mv |
EDUFU |
publisher.none.fl_str_mv |
EDUFU |
dc.source.none.fl_str_mv |
Bioscience Journal ; Vol. 36 No. 5 (2020): Sept./Oct.; 1750-1759 Bioscience Journal ; v. 36 n. 5 (2020): Sept./Oct.; 1750-1759 1981-3163 reponame:Bioscience journal (Online) instname:Universidade Federal de Uberlândia (UFU) instacron:UFU |
instname_str |
Universidade Federal de Uberlândia (UFU) |
instacron_str |
UFU |
institution |
UFU |
reponame_str |
Bioscience journal (Online) |
collection |
Bioscience journal (Online) |
repository.name.fl_str_mv |
Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU) |
repository.mail.fl_str_mv |
biosciencej@ufu.br|| |
_version_ |
1797069082345865216 |