Process optimization for enhanced production of cellulases form locally isolated fungal strain by submerged fermentation

Detalhes bibliográficos
Autor(a) principal: Abdullah, Roheena
Data de Publicação: 2021
Outros Autores: Akhtar, Ammara, Nisar, Kinza, Kaleem, Afshan, Iqtedar, Mehwish, Iftikhar, Tehreema, Saleem, Faiza, Aslam, Farheen
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Bioscience journal (Online)
Texto Completo: https://seer.ufu.br/index.php/biosciencejournal/article/view/53815
Resumo: Cellulase has myriad applications in various sectors like pharmaceuticals, textile, detergents, animal feed and bioethanol production, etc. The current study focuses on the isolation, screening and optimization of fungal strain through one factor at a time technique for enhanced cellulase production.  In current study sixteen different fungal cultures were isolated and the culture which quantitatively exhibits higher titers of cellulase activity was identified both morphologically and molecularly by 18S rDNA and designated as Aspergillus niger ABT11. Different parameters like fermentation medium, volume, temperature, pH and nutritional components were optimized. The highest CMCase and FPase activities  was achieved in 100ml of M5 medium in the presence of 1% lactose and sodium nitrate at 30 oC, pH5 after 72 hours. The result revealed A. niger can be a potential candidate for scale up studies.
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spelling Process optimization for enhanced production of cellulases form locally isolated fungal strain by submerged fermentationAspergillus nigerCellulaseCMCaseFPaseMolecular CharacterizationBiological SciencesCellulase has myriad applications in various sectors like pharmaceuticals, textile, detergents, animal feed and bioethanol production, etc. The current study focuses on the isolation, screening and optimization of fungal strain through one factor at a time technique for enhanced cellulase production.  In current study sixteen different fungal cultures were isolated and the culture which quantitatively exhibits higher titers of cellulase activity was identified both morphologically and molecularly by 18S rDNA and designated as Aspergillus niger ABT11. Different parameters like fermentation medium, volume, temperature, pH and nutritional components were optimized. The highest CMCase and FPase activities  was achieved in 100ml of M5 medium in the presence of 1% lactose and sodium nitrate at 30 oC, pH5 after 72 hours. The result revealed A. niger can be a potential candidate for scale up studies.EDUFU2021-05-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/5381510.14393/BJ-v37n0a2021-53815Bioscience Journal ; Vol. 37 (2021): Continuous Publication; e37021Bioscience Journal ; v. 37 (2021): Continuous Publication; e370211981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/53815/31536Pakistan; ContemporaryCopyright (c) 2021 Roheena Abdullah, Ammara Akhtar, Kinza Nisar, Afshan Kaleem, Mehwish Iqtedar, Tehreema Iftikhar, Faiza Saleem, Farheen Aslamhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessAbdullah, RoheenaAkhtar, AmmaraNisar, KinzaKaleem, Afshan Iqtedar, MehwishIftikhar, TehreemaSaleem, FaizaAslam, Farheen 2022-05-25T13:43:17Zoai:ojs.www.seer.ufu.br:article/53815Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2022-05-25T13:43:17Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false
dc.title.none.fl_str_mv Process optimization for enhanced production of cellulases form locally isolated fungal strain by submerged fermentation
title Process optimization for enhanced production of cellulases form locally isolated fungal strain by submerged fermentation
spellingShingle Process optimization for enhanced production of cellulases form locally isolated fungal strain by submerged fermentation
Abdullah, Roheena
Aspergillus niger
Cellulase
CMCase
FPase
Molecular Characterization
Biological Sciences
title_short Process optimization for enhanced production of cellulases form locally isolated fungal strain by submerged fermentation
title_full Process optimization for enhanced production of cellulases form locally isolated fungal strain by submerged fermentation
title_fullStr Process optimization for enhanced production of cellulases form locally isolated fungal strain by submerged fermentation
title_full_unstemmed Process optimization for enhanced production of cellulases form locally isolated fungal strain by submerged fermentation
title_sort Process optimization for enhanced production of cellulases form locally isolated fungal strain by submerged fermentation
author Abdullah, Roheena
author_facet Abdullah, Roheena
Akhtar, Ammara
Nisar, Kinza
Kaleem, Afshan
Iqtedar, Mehwish
Iftikhar, Tehreema
Saleem, Faiza
Aslam, Farheen
author_role author
author2 Akhtar, Ammara
Nisar, Kinza
Kaleem, Afshan
Iqtedar, Mehwish
Iftikhar, Tehreema
Saleem, Faiza
Aslam, Farheen
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Abdullah, Roheena
Akhtar, Ammara
Nisar, Kinza
Kaleem, Afshan
Iqtedar, Mehwish
Iftikhar, Tehreema
Saleem, Faiza
Aslam, Farheen
dc.subject.por.fl_str_mv Aspergillus niger
Cellulase
CMCase
FPase
Molecular Characterization
Biological Sciences
topic Aspergillus niger
Cellulase
CMCase
FPase
Molecular Characterization
Biological Sciences
description Cellulase has myriad applications in various sectors like pharmaceuticals, textile, detergents, animal feed and bioethanol production, etc. The current study focuses on the isolation, screening and optimization of fungal strain through one factor at a time technique for enhanced cellulase production.  In current study sixteen different fungal cultures were isolated and the culture which quantitatively exhibits higher titers of cellulase activity was identified both morphologically and molecularly by 18S rDNA and designated as Aspergillus niger ABT11. Different parameters like fermentation medium, volume, temperature, pH and nutritional components were optimized. The highest CMCase and FPase activities  was achieved in 100ml of M5 medium in the presence of 1% lactose and sodium nitrate at 30 oC, pH5 after 72 hours. The result revealed A. niger can be a potential candidate for scale up studies.
publishDate 2021
dc.date.none.fl_str_mv 2021-05-12
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.ufu.br/index.php/biosciencejournal/article/view/53815
10.14393/BJ-v37n0a2021-53815
url https://seer.ufu.br/index.php/biosciencejournal/article/view/53815
identifier_str_mv 10.14393/BJ-v37n0a2021-53815
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.ufu.br/index.php/biosciencejournal/article/view/53815/31536
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Pakistan; Contemporary
dc.publisher.none.fl_str_mv EDUFU
publisher.none.fl_str_mv EDUFU
dc.source.none.fl_str_mv Bioscience Journal ; Vol. 37 (2021): Continuous Publication; e37021
Bioscience Journal ; v. 37 (2021): Continuous Publication; e37021
1981-3163
reponame:Bioscience journal (Online)
instname:Universidade Federal de Uberlândia (UFU)
instacron:UFU
instname_str Universidade Federal de Uberlândia (UFU)
instacron_str UFU
institution UFU
reponame_str Bioscience journal (Online)
collection Bioscience journal (Online)
repository.name.fl_str_mv Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)
repository.mail.fl_str_mv biosciencej@ufu.br||
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