Identification of polymorphisms in the myostatin and leptin genes of santa inês breed and crossbreed sheep and association with carcass traits
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Bioscience journal (Online) |
Texto Completo: | https://seer.ufu.br/index.php/biosciencejournal/article/view/30184 |
Resumo: | The objective of this study was to identify polymorphisms in the myostatin and leptin genes in Santa Inês (SI) and crossbreed (SI x Dorper) sheep, to verify the effect of these polymorphisms on carcass traits. We evaluated seventy sheep of 8-month-old at the Federal Institute of Espírito Santo, Brazil. Data collected were slaughter weight (SW), hot carcass weight (HCW), cold carcass weight (CCW), loin weight, tenderloin weight and fat thickness (FT). The hot carcass yield (HCY) was calculated by the formula (HCW/SW) x 100. We collected hairs from each animal for DNA extraction by the alkaline protocol. The animals were genotyped for the G>A mutation in nucleotide 9827 of the myostatin gene and for three polymorphisms in exon 3 of the leptin gene, by the PCR-RFLP technique. The amplicons the myostatin and leptin gene were cleaved with restriction enzyme for allelic discrimination. The alleles were recorded for each animal and analysis of variance was performed to check the influence of the mutations on the carcass traits. The mutant allele of the myostatin gene showed association with increased measures of CCW, FT and with reduced HCY. Among the three alleles of the leptin gene, only one showed an effect (increased CCW). The other alleles were not associated with any traits. |
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Identification of polymorphisms in the myostatin and leptin genes of santa inês breed and crossbreed sheep and association with carcass traits fat thicknessmutationlambmyostatinleptinAgricultural SciencesThe objective of this study was to identify polymorphisms in the myostatin and leptin genes in Santa Inês (SI) and crossbreed (SI x Dorper) sheep, to verify the effect of these polymorphisms on carcass traits. We evaluated seventy sheep of 8-month-old at the Federal Institute of Espírito Santo, Brazil. Data collected were slaughter weight (SW), hot carcass weight (HCW), cold carcass weight (CCW), loin weight, tenderloin weight and fat thickness (FT). The hot carcass yield (HCY) was calculated by the formula (HCW/SW) x 100. We collected hairs from each animal for DNA extraction by the alkaline protocol. The animals were genotyped for the G>A mutation in nucleotide 9827 of the myostatin gene and for three polymorphisms in exon 3 of the leptin gene, by the PCR-RFLP technique. The amplicons the myostatin and leptin gene were cleaved with restriction enzyme for allelic discrimination. The alleles were recorded for each animal and analysis of variance was performed to check the influence of the mutations on the carcass traits. The mutant allele of the myostatin gene showed association with increased measures of CCW, FT and with reduced HCY. Among the three alleles of the leptin gene, only one showed an effect (increased CCW). The other alleles were not associated with any traits.EDUFU2016-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/3018410.14393/BJ-v32n3a2016-30184Bioscience Journal ; Vol. 32 No. 3 (2016): May/June; 699-704Bioscience Journal ; v. 32 n. 3 (2016): May/June; 699-7041981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/30184/18326Brazil; ContemporaryCopyright (c) 2016 Celia Raquel Quirino, Ricardo Lopes Dias da Costa, Aline Pacheco, Aparecida de Fátima Madella-Oliveira, Renato Travassos Beltrame, Amanda da Silva Azevedo, Aylton Bartholazzi Junior, Wilder Hernando Ortiz Vegahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessQuirino, Celia Raquelda Costa, Ricardo Lopes DiasPacheco, AlineMadella-Oliveira, Aparecida de FátimaBeltrame, Renato TravassosAzevedo, Amanda da SilvaBartholazzi Junior, AyltonVega, Wilder Hernando Ortiz2022-05-17T02:21:15Zoai:ojs.www.seer.ufu.br:article/30184Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2022-05-17T02:21:15Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false |
dc.title.none.fl_str_mv |
Identification of polymorphisms in the myostatin and leptin genes of santa inês breed and crossbreed sheep and association with carcass traits |
title |
Identification of polymorphisms in the myostatin and leptin genes of santa inês breed and crossbreed sheep and association with carcass traits |
spellingShingle |
Identification of polymorphisms in the myostatin and leptin genes of santa inês breed and crossbreed sheep and association with carcass traits Quirino, Celia Raquel fat thickness mutation lamb myostatin leptin Agricultural Sciences |
title_short |
Identification of polymorphisms in the myostatin and leptin genes of santa inês breed and crossbreed sheep and association with carcass traits |
title_full |
Identification of polymorphisms in the myostatin and leptin genes of santa inês breed and crossbreed sheep and association with carcass traits |
title_fullStr |
Identification of polymorphisms in the myostatin and leptin genes of santa inês breed and crossbreed sheep and association with carcass traits |
title_full_unstemmed |
Identification of polymorphisms in the myostatin and leptin genes of santa inês breed and crossbreed sheep and association with carcass traits |
title_sort |
Identification of polymorphisms in the myostatin and leptin genes of santa inês breed and crossbreed sheep and association with carcass traits |
author |
Quirino, Celia Raquel |
author_facet |
Quirino, Celia Raquel da Costa, Ricardo Lopes Dias Pacheco, Aline Madella-Oliveira, Aparecida de Fátima Beltrame, Renato Travassos Azevedo, Amanda da Silva Bartholazzi Junior, Aylton Vega, Wilder Hernando Ortiz |
author_role |
author |
author2 |
da Costa, Ricardo Lopes Dias Pacheco, Aline Madella-Oliveira, Aparecida de Fátima Beltrame, Renato Travassos Azevedo, Amanda da Silva Bartholazzi Junior, Aylton Vega, Wilder Hernando Ortiz |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Quirino, Celia Raquel da Costa, Ricardo Lopes Dias Pacheco, Aline Madella-Oliveira, Aparecida de Fátima Beltrame, Renato Travassos Azevedo, Amanda da Silva Bartholazzi Junior, Aylton Vega, Wilder Hernando Ortiz |
dc.subject.por.fl_str_mv |
fat thickness mutation lamb myostatin leptin Agricultural Sciences |
topic |
fat thickness mutation lamb myostatin leptin Agricultural Sciences |
description |
The objective of this study was to identify polymorphisms in the myostatin and leptin genes in Santa Inês (SI) and crossbreed (SI x Dorper) sheep, to verify the effect of these polymorphisms on carcass traits. We evaluated seventy sheep of 8-month-old at the Federal Institute of Espírito Santo, Brazil. Data collected were slaughter weight (SW), hot carcass weight (HCW), cold carcass weight (CCW), loin weight, tenderloin weight and fat thickness (FT). The hot carcass yield (HCY) was calculated by the formula (HCW/SW) x 100. We collected hairs from each animal for DNA extraction by the alkaline protocol. The animals were genotyped for the G>A mutation in nucleotide 9827 of the myostatin gene and for three polymorphisms in exon 3 of the leptin gene, by the PCR-RFLP technique. The amplicons the myostatin and leptin gene were cleaved with restriction enzyme for allelic discrimination. The alleles were recorded for each animal and analysis of variance was performed to check the influence of the mutations on the carcass traits. The mutant allele of the myostatin gene showed association with increased measures of CCW, FT and with reduced HCY. Among the three alleles of the leptin gene, only one showed an effect (increased CCW). The other alleles were not associated with any traits. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/30184 10.14393/BJ-v32n3a2016-30184 |
url |
https://seer.ufu.br/index.php/biosciencejournal/article/view/30184 |
identifier_str_mv |
10.14393/BJ-v32n3a2016-30184 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/30184/18326 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
Brazil; Contemporary |
dc.publisher.none.fl_str_mv |
EDUFU |
publisher.none.fl_str_mv |
EDUFU |
dc.source.none.fl_str_mv |
Bioscience Journal ; Vol. 32 No. 3 (2016): May/June; 699-704 Bioscience Journal ; v. 32 n. 3 (2016): May/June; 699-704 1981-3163 reponame:Bioscience journal (Online) instname:Universidade Federal de Uberlândia (UFU) instacron:UFU |
instname_str |
Universidade Federal de Uberlândia (UFU) |
instacron_str |
UFU |
institution |
UFU |
reponame_str |
Bioscience journal (Online) |
collection |
Bioscience journal (Online) |
repository.name.fl_str_mv |
Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU) |
repository.mail.fl_str_mv |
biosciencej@ufu.br|| |
_version_ |
1797069075865665536 |