Molecular analysis of the prevalence of Acinetobacter baumannii in hospitals and the surrounding environments: a cross-sectional study

Detalhes bibliográficos
Autor(a) principal: Silva, Kerollyn Fernandes Bernardes
Data de Publicação: 2023
Outros Autores: Miguel, Camila Botelho, Oliveira Júnior, Rony Rocha de, Araujo, Marcelo Costa, Agostinho, Ferdinando, Ueira-Vieira, Carlos, Lazo-Chica, Javier Emilio, Rodrigues, Wellington
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Bioscience journal (Online)
Texto Completo: https://seer.ufu.br/index.php/biosciencejournal/article/view/63071
Resumo: Acinetobacter baumannii is widely recognized in clinical environments due to its infectious capacity, antimicrobial adaptability, and lethality. Analyzing the prevalence of this agent in intra- and extra-hospital environments may reveal target indicators for appropriate management interventions. In this observational cross-sectional study, we evaluated the prevalence of A. baumannii within hospitals with intensive care units and in their external surroundings in a macro-health region of Brazil. Samples of Columba livia (pigeon) droppings from the external environment of four hospitals (n = 40), from floor surfaces (n = 20), and door handles (n = 20) of different hospital wards were collected based on random sampling, all of which were evaluated for the presence of A. baumannii using polymerase chain reactions (PCR). The sensitivity and specificity of the technique was verified after the collected samples were contaminated with clinical samples positive for A. baumannii. We detected a significantly higher A. baumannii prevalence (87.50%, CI = 71.29–100.00) in samples collected within the hospital environment compared with those obtained from the external environment (12.50%, CI = 0.00–28. 71) (p = 0.003). In addition, samples collected from floor surfaces contained bacterial densities (181.3 ± 11.58) that exceeded those in environmental (93.32 ± 1.56) and door handle (142.70 ± 17.14) samples by 94% and 78.71%, respectively. The findings of this study will enhance our understanding of the spatial distribution of A. baumannii and additionally, validate the efficiency of PCR for diagnosis of this infectious agent.
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spelling Molecular analysis of the prevalence of Acinetobacter baumannii in hospitals and the surrounding environments: a cross-sectional studyAcinetobacter baumanniiEnvironmentHospital InfectionPolymerase Chain Reaction. Health SciencesAcinetobacter baumannii is widely recognized in clinical environments due to its infectious capacity, antimicrobial adaptability, and lethality. Analyzing the prevalence of this agent in intra- and extra-hospital environments may reveal target indicators for appropriate management interventions. In this observational cross-sectional study, we evaluated the prevalence of A. baumannii within hospitals with intensive care units and in their external surroundings in a macro-health region of Brazil. Samples of Columba livia (pigeon) droppings from the external environment of four hospitals (n = 40), from floor surfaces (n = 20), and door handles (n = 20) of different hospital wards were collected based on random sampling, all of which were evaluated for the presence of A. baumannii using polymerase chain reactions (PCR). The sensitivity and specificity of the technique was verified after the collected samples were contaminated with clinical samples positive for A. baumannii. We detected a significantly higher A. baumannii prevalence (87.50%, CI = 71.29–100.00) in samples collected within the hospital environment compared with those obtained from the external environment (12.50%, CI = 0.00–28. 71) (p = 0.003). In addition, samples collected from floor surfaces contained bacterial densities (181.3 ± 11.58) that exceeded those in environmental (93.32 ± 1.56) and door handle (142.70 ± 17.14) samples by 94% and 78.71%, respectively. The findings of this study will enhance our understanding of the spatial distribution of A. baumannii and additionally, validate the efficiency of PCR for diagnosis of this infectious agent.Universidade Federal de Uberlândia2023-01-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/6307110.14393/BJ-v39n0a2023-63071Bioscience Journal ; Vol. 39 (2023): Continuous Publication; e39019Bioscience Journal ; v. 39 (2023): Continuous Publication; e390191981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/63071/35510Brazil; Contemporary Copyright (c) 2023 Kerollyn Fernandes Bernardes Silva, Camila Botelho Miguel, Rony Rocha de Oliveira Júnior, Marcelo Costa Araujo, Ferdinando Agostinho, Carlos Ueira-Vieira, Javier Emilio Lazo-Chica, Wellington Rodrigueshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccess Silva, Kerollyn Fernandes BernardesMiguel, Camila BotelhoOliveira Júnior, Rony Rocha deAraujo, Marcelo CostaAgostinho, FerdinandoUeira-Vieira, CarlosLazo-Chica, Javier EmilioRodrigues, Wellington2024-01-31T19:16:18Zoai:ojs.www.seer.ufu.br:article/63071Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2024-01-31T19:16:18Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false
dc.title.none.fl_str_mv Molecular analysis of the prevalence of Acinetobacter baumannii in hospitals and the surrounding environments: a cross-sectional study
title Molecular analysis of the prevalence of Acinetobacter baumannii in hospitals and the surrounding environments: a cross-sectional study
spellingShingle Molecular analysis of the prevalence of Acinetobacter baumannii in hospitals and the surrounding environments: a cross-sectional study
Silva, Kerollyn Fernandes Bernardes
Acinetobacter baumannii
Environment
Hospital Infection
Polymerase Chain Reaction.
Health Sciences
title_short Molecular analysis of the prevalence of Acinetobacter baumannii in hospitals and the surrounding environments: a cross-sectional study
title_full Molecular analysis of the prevalence of Acinetobacter baumannii in hospitals and the surrounding environments: a cross-sectional study
title_fullStr Molecular analysis of the prevalence of Acinetobacter baumannii in hospitals and the surrounding environments: a cross-sectional study
title_full_unstemmed Molecular analysis of the prevalence of Acinetobacter baumannii in hospitals and the surrounding environments: a cross-sectional study
title_sort Molecular analysis of the prevalence of Acinetobacter baumannii in hospitals and the surrounding environments: a cross-sectional study
author Silva, Kerollyn Fernandes Bernardes
author_facet Silva, Kerollyn Fernandes Bernardes
Miguel, Camila Botelho
Oliveira Júnior, Rony Rocha de
Araujo, Marcelo Costa
Agostinho, Ferdinando
Ueira-Vieira, Carlos
Lazo-Chica, Javier Emilio
Rodrigues, Wellington
author_role author
author2 Miguel, Camila Botelho
Oliveira Júnior, Rony Rocha de
Araujo, Marcelo Costa
Agostinho, Ferdinando
Ueira-Vieira, Carlos
Lazo-Chica, Javier Emilio
Rodrigues, Wellington
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Silva, Kerollyn Fernandes Bernardes
Miguel, Camila Botelho
Oliveira Júnior, Rony Rocha de
Araujo, Marcelo Costa
Agostinho, Ferdinando
Ueira-Vieira, Carlos
Lazo-Chica, Javier Emilio
Rodrigues, Wellington
dc.subject.por.fl_str_mv Acinetobacter baumannii
Environment
Hospital Infection
Polymerase Chain Reaction.
Health Sciences
topic Acinetobacter baumannii
Environment
Hospital Infection
Polymerase Chain Reaction.
Health Sciences
description Acinetobacter baumannii is widely recognized in clinical environments due to its infectious capacity, antimicrobial adaptability, and lethality. Analyzing the prevalence of this agent in intra- and extra-hospital environments may reveal target indicators for appropriate management interventions. In this observational cross-sectional study, we evaluated the prevalence of A. baumannii within hospitals with intensive care units and in their external surroundings in a macro-health region of Brazil. Samples of Columba livia (pigeon) droppings from the external environment of four hospitals (n = 40), from floor surfaces (n = 20), and door handles (n = 20) of different hospital wards were collected based on random sampling, all of which were evaluated for the presence of A. baumannii using polymerase chain reactions (PCR). The sensitivity and specificity of the technique was verified after the collected samples were contaminated with clinical samples positive for A. baumannii. We detected a significantly higher A. baumannii prevalence (87.50%, CI = 71.29–100.00) in samples collected within the hospital environment compared with those obtained from the external environment (12.50%, CI = 0.00–28. 71) (p = 0.003). In addition, samples collected from floor surfaces contained bacterial densities (181.3 ± 11.58) that exceeded those in environmental (93.32 ± 1.56) and door handle (142.70 ± 17.14) samples by 94% and 78.71%, respectively. The findings of this study will enhance our understanding of the spatial distribution of A. baumannii and additionally, validate the efficiency of PCR for diagnosis of this infectious agent.
publishDate 2023
dc.date.none.fl_str_mv 2023-01-27
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.ufu.br/index.php/biosciencejournal/article/view/63071
10.14393/BJ-v39n0a2023-63071
url https://seer.ufu.br/index.php/biosciencejournal/article/view/63071
identifier_str_mv 10.14393/BJ-v39n0a2023-63071
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.ufu.br/index.php/biosciencejournal/article/view/63071/35510
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Brazil; Contemporary
dc.publisher.none.fl_str_mv Universidade Federal de Uberlândia
publisher.none.fl_str_mv Universidade Federal de Uberlândia
dc.source.none.fl_str_mv Bioscience Journal ; Vol. 39 (2023): Continuous Publication; e39019
Bioscience Journal ; v. 39 (2023): Continuous Publication; e39019
1981-3163
reponame:Bioscience journal (Online)
instname:Universidade Federal de Uberlândia (UFU)
instacron:UFU
instname_str Universidade Federal de Uberlândia (UFU)
instacron_str UFU
institution UFU
reponame_str Bioscience journal (Online)
collection Bioscience journal (Online)
repository.name.fl_str_mv Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)
repository.mail.fl_str_mv biosciencej@ufu.br||
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