Molecular analysis of the prevalence of Acinetobacter baumannii in hospitals and the surrounding environments: a cross-sectional study
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Bioscience journal (Online) |
Texto Completo: | https://seer.ufu.br/index.php/biosciencejournal/article/view/63071 |
Resumo: | Acinetobacter baumannii is widely recognized in clinical environments due to its infectious capacity, antimicrobial adaptability, and lethality. Analyzing the prevalence of this agent in intra- and extra-hospital environments may reveal target indicators for appropriate management interventions. In this observational cross-sectional study, we evaluated the prevalence of A. baumannii within hospitals with intensive care units and in their external surroundings in a macro-health region of Brazil. Samples of Columba livia (pigeon) droppings from the external environment of four hospitals (n = 40), from floor surfaces (n = 20), and door handles (n = 20) of different hospital wards were collected based on random sampling, all of which were evaluated for the presence of A. baumannii using polymerase chain reactions (PCR). The sensitivity and specificity of the technique was verified after the collected samples were contaminated with clinical samples positive for A. baumannii. We detected a significantly higher A. baumannii prevalence (87.50%, CI = 71.29–100.00) in samples collected within the hospital environment compared with those obtained from the external environment (12.50%, CI = 0.00–28. 71) (p = 0.003). In addition, samples collected from floor surfaces contained bacterial densities (181.3 ± 11.58) that exceeded those in environmental (93.32 ± 1.56) and door handle (142.70 ± 17.14) samples by 94% and 78.71%, respectively. The findings of this study will enhance our understanding of the spatial distribution of A. baumannii and additionally, validate the efficiency of PCR for diagnosis of this infectious agent. |
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Molecular analysis of the prevalence of Acinetobacter baumannii in hospitals and the surrounding environments: a cross-sectional studyAcinetobacter baumanniiEnvironmentHospital InfectionPolymerase Chain Reaction. Health SciencesAcinetobacter baumannii is widely recognized in clinical environments due to its infectious capacity, antimicrobial adaptability, and lethality. Analyzing the prevalence of this agent in intra- and extra-hospital environments may reveal target indicators for appropriate management interventions. In this observational cross-sectional study, we evaluated the prevalence of A. baumannii within hospitals with intensive care units and in their external surroundings in a macro-health region of Brazil. Samples of Columba livia (pigeon) droppings from the external environment of four hospitals (n = 40), from floor surfaces (n = 20), and door handles (n = 20) of different hospital wards were collected based on random sampling, all of which were evaluated for the presence of A. baumannii using polymerase chain reactions (PCR). The sensitivity and specificity of the technique was verified after the collected samples were contaminated with clinical samples positive for A. baumannii. We detected a significantly higher A. baumannii prevalence (87.50%, CI = 71.29–100.00) in samples collected within the hospital environment compared with those obtained from the external environment (12.50%, CI = 0.00–28. 71) (p = 0.003). In addition, samples collected from floor surfaces contained bacterial densities (181.3 ± 11.58) that exceeded those in environmental (93.32 ± 1.56) and door handle (142.70 ± 17.14) samples by 94% and 78.71%, respectively. The findings of this study will enhance our understanding of the spatial distribution of A. baumannii and additionally, validate the efficiency of PCR for diagnosis of this infectious agent.Universidade Federal de Uberlândia2023-01-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/6307110.14393/BJ-v39n0a2023-63071Bioscience Journal ; Vol. 39 (2023): Continuous Publication; e39019Bioscience Journal ; v. 39 (2023): Continuous Publication; e390191981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/63071/35510Brazil; Contemporary Copyright (c) 2023 Kerollyn Fernandes Bernardes Silva, Camila Botelho Miguel, Rony Rocha de Oliveira Júnior, Marcelo Costa Araujo, Ferdinando Agostinho, Carlos Ueira-Vieira, Javier Emilio Lazo-Chica, Wellington Rodrigueshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccess Silva, Kerollyn Fernandes BernardesMiguel, Camila BotelhoOliveira Júnior, Rony Rocha deAraujo, Marcelo CostaAgostinho, FerdinandoUeira-Vieira, CarlosLazo-Chica, Javier EmilioRodrigues, Wellington2024-01-31T19:16:18Zoai:ojs.www.seer.ufu.br:article/63071Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2024-01-31T19:16:18Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false |
dc.title.none.fl_str_mv |
Molecular analysis of the prevalence of Acinetobacter baumannii in hospitals and the surrounding environments: a cross-sectional study |
title |
Molecular analysis of the prevalence of Acinetobacter baumannii in hospitals and the surrounding environments: a cross-sectional study |
spellingShingle |
Molecular analysis of the prevalence of Acinetobacter baumannii in hospitals and the surrounding environments: a cross-sectional study Silva, Kerollyn Fernandes Bernardes Acinetobacter baumannii Environment Hospital Infection Polymerase Chain Reaction. Health Sciences |
title_short |
Molecular analysis of the prevalence of Acinetobacter baumannii in hospitals and the surrounding environments: a cross-sectional study |
title_full |
Molecular analysis of the prevalence of Acinetobacter baumannii in hospitals and the surrounding environments: a cross-sectional study |
title_fullStr |
Molecular analysis of the prevalence of Acinetobacter baumannii in hospitals and the surrounding environments: a cross-sectional study |
title_full_unstemmed |
Molecular analysis of the prevalence of Acinetobacter baumannii in hospitals and the surrounding environments: a cross-sectional study |
title_sort |
Molecular analysis of the prevalence of Acinetobacter baumannii in hospitals and the surrounding environments: a cross-sectional study |
author |
Silva, Kerollyn Fernandes Bernardes |
author_facet |
Silva, Kerollyn Fernandes Bernardes Miguel, Camila Botelho Oliveira Júnior, Rony Rocha de Araujo, Marcelo Costa Agostinho, Ferdinando Ueira-Vieira, Carlos Lazo-Chica, Javier Emilio Rodrigues, Wellington |
author_role |
author |
author2 |
Miguel, Camila Botelho Oliveira Júnior, Rony Rocha de Araujo, Marcelo Costa Agostinho, Ferdinando Ueira-Vieira, Carlos Lazo-Chica, Javier Emilio Rodrigues, Wellington |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Silva, Kerollyn Fernandes Bernardes Miguel, Camila Botelho Oliveira Júnior, Rony Rocha de Araujo, Marcelo Costa Agostinho, Ferdinando Ueira-Vieira, Carlos Lazo-Chica, Javier Emilio Rodrigues, Wellington |
dc.subject.por.fl_str_mv |
Acinetobacter baumannii Environment Hospital Infection Polymerase Chain Reaction. Health Sciences |
topic |
Acinetobacter baumannii Environment Hospital Infection Polymerase Chain Reaction. Health Sciences |
description |
Acinetobacter baumannii is widely recognized in clinical environments due to its infectious capacity, antimicrobial adaptability, and lethality. Analyzing the prevalence of this agent in intra- and extra-hospital environments may reveal target indicators for appropriate management interventions. In this observational cross-sectional study, we evaluated the prevalence of A. baumannii within hospitals with intensive care units and in their external surroundings in a macro-health region of Brazil. Samples of Columba livia (pigeon) droppings from the external environment of four hospitals (n = 40), from floor surfaces (n = 20), and door handles (n = 20) of different hospital wards were collected based on random sampling, all of which were evaluated for the presence of A. baumannii using polymerase chain reactions (PCR). The sensitivity and specificity of the technique was verified after the collected samples were contaminated with clinical samples positive for A. baumannii. We detected a significantly higher A. baumannii prevalence (87.50%, CI = 71.29–100.00) in samples collected within the hospital environment compared with those obtained from the external environment (12.50%, CI = 0.00–28. 71) (p = 0.003). In addition, samples collected from floor surfaces contained bacterial densities (181.3 ± 11.58) that exceeded those in environmental (93.32 ± 1.56) and door handle (142.70 ± 17.14) samples by 94% and 78.71%, respectively. The findings of this study will enhance our understanding of the spatial distribution of A. baumannii and additionally, validate the efficiency of PCR for diagnosis of this infectious agent. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-01-27 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/63071 10.14393/BJ-v39n0a2023-63071 |
url |
https://seer.ufu.br/index.php/biosciencejournal/article/view/63071 |
identifier_str_mv |
10.14393/BJ-v39n0a2023-63071 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/63071/35510 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
Brazil; Contemporary |
dc.publisher.none.fl_str_mv |
Universidade Federal de Uberlândia |
publisher.none.fl_str_mv |
Universidade Federal de Uberlândia |
dc.source.none.fl_str_mv |
Bioscience Journal ; Vol. 39 (2023): Continuous Publication; e39019 Bioscience Journal ; v. 39 (2023): Continuous Publication; e39019 1981-3163 reponame:Bioscience journal (Online) instname:Universidade Federal de Uberlândia (UFU) instacron:UFU |
instname_str |
Universidade Federal de Uberlândia (UFU) |
instacron_str |
UFU |
institution |
UFU |
reponame_str |
Bioscience journal (Online) |
collection |
Bioscience journal (Online) |
repository.name.fl_str_mv |
Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU) |
repository.mail.fl_str_mv |
biosciencej@ufu.br|| |
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1797069065185918976 |