Cryopreservation of Agaricus blazei in liquid nitrogen using DMSO as cryoprotectant
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Bioscience journal (Online) |
Texto Completo: | https://seer.ufu.br/index.php/biosciencejournal/article/view/14114 |
Resumo: | The preservation of Agaricus blazei is generally done using successive subcultivations that are laborious and are subject to contaminations or genetic degenerations, resulting in loss of biotechnological interest characteristics. An alternative process would be cryopreservation, but there are no reports of methodologies for this basidiomycete in liquid nitrogen. Thus, the objective of this study was to evaluate mycelial viability of A. blazei strains after cryopreservation in liquid nitrogen in order to establish the initial parameters of species preservation. Five strains grown on malt extract agar (MEA) were used. Disks of MEA containing A. blazei mycelium were transferred for screw-cap cryovials containing the cryoprotectant, 10% dimethyl sulfoxide. Then, they were cooled at 8 ºC for 30 min and kept at -196 ºC with liquid nitrogen. After 1.5 year of cryopreservation, the cryovials were thawed in water bath at 30 ºC for 15 min. The disks with mycelia were transferred to MEA culture media without cryoprotectant and kept at 28 ºC for 30 days. A. blazei strains respond differently to the cryopreservation method at -196 ºC by varying mycelial viability recovery. Cryopreservation with liquid nitrogen, using dimethyl sulfoxide as cryoprotectant, is not the most appropriate one for A. blazei preservation. |
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Cryopreservation of Agaricus blazei in liquid nitrogen using DMSO as cryoprotectant Biological SciencesThe preservation of Agaricus blazei is generally done using successive subcultivations that are laborious and are subject to contaminations or genetic degenerations, resulting in loss of biotechnological interest characteristics. An alternative process would be cryopreservation, but there are no reports of methodologies for this basidiomycete in liquid nitrogen. Thus, the objective of this study was to evaluate mycelial viability of A. blazei strains after cryopreservation in liquid nitrogen in order to establish the initial parameters of species preservation. Five strains grown on malt extract agar (MEA) were used. Disks of MEA containing A. blazei mycelium were transferred for screw-cap cryovials containing the cryoprotectant, 10% dimethyl sulfoxide. Then, they were cooled at 8 ºC for 30 min and kept at -196 ºC with liquid nitrogen. After 1.5 year of cryopreservation, the cryovials were thawed in water bath at 30 ºC for 15 min. The disks with mycelia were transferred to MEA culture media without cryoprotectant and kept at 28 ºC for 30 days. A. blazei strains respond differently to the cryopreservation method at -196 ºC by varying mycelial viability recovery. Cryopreservation with liquid nitrogen, using dimethyl sulfoxide as cryoprotectant, is not the most appropriate one for A. blazei preservation.EDUFU2012-12-21info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/14114Bioscience Journal ; Vol. 28 No. 6 (2012): Nov./Dec.; 1034-1037Bioscience Journal ; v. 28 n. 6 (2012): Nov./Dec.; 1034-10371981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUporhttps://seer.ufu.br/index.php/biosciencejournal/article/view/14114/11129Brazil; ContemporanyCopyright (c) 2012 Nelson Barros Colauto, Augusto Ferreira da Eira, Giani Andrea Lindehttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessColauto, Nelson BarrosEira, Augusto Ferreira daLinde, Giani Andrea2022-06-01T17:54:14Zoai:ojs.www.seer.ufu.br:article/14114Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2022-06-01T17:54:14Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false |
dc.title.none.fl_str_mv |
Cryopreservation of Agaricus blazei in liquid nitrogen using DMSO as cryoprotectant |
title |
Cryopreservation of Agaricus blazei in liquid nitrogen using DMSO as cryoprotectant |
spellingShingle |
Cryopreservation of Agaricus blazei in liquid nitrogen using DMSO as cryoprotectant Colauto, Nelson Barros Biological Sciences |
title_short |
Cryopreservation of Agaricus blazei in liquid nitrogen using DMSO as cryoprotectant |
title_full |
Cryopreservation of Agaricus blazei in liquid nitrogen using DMSO as cryoprotectant |
title_fullStr |
Cryopreservation of Agaricus blazei in liquid nitrogen using DMSO as cryoprotectant |
title_full_unstemmed |
Cryopreservation of Agaricus blazei in liquid nitrogen using DMSO as cryoprotectant |
title_sort |
Cryopreservation of Agaricus blazei in liquid nitrogen using DMSO as cryoprotectant |
author |
Colauto, Nelson Barros |
author_facet |
Colauto, Nelson Barros Eira, Augusto Ferreira da Linde, Giani Andrea |
author_role |
author |
author2 |
Eira, Augusto Ferreira da Linde, Giani Andrea |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Colauto, Nelson Barros Eira, Augusto Ferreira da Linde, Giani Andrea |
dc.subject.por.fl_str_mv |
Biological Sciences |
topic |
Biological Sciences |
description |
The preservation of Agaricus blazei is generally done using successive subcultivations that are laborious and are subject to contaminations or genetic degenerations, resulting in loss of biotechnological interest characteristics. An alternative process would be cryopreservation, but there are no reports of methodologies for this basidiomycete in liquid nitrogen. Thus, the objective of this study was to evaluate mycelial viability of A. blazei strains after cryopreservation in liquid nitrogen in order to establish the initial parameters of species preservation. Five strains grown on malt extract agar (MEA) were used. Disks of MEA containing A. blazei mycelium were transferred for screw-cap cryovials containing the cryoprotectant, 10% dimethyl sulfoxide. Then, they were cooled at 8 ºC for 30 min and kept at -196 ºC with liquid nitrogen. After 1.5 year of cryopreservation, the cryovials were thawed in water bath at 30 ºC for 15 min. The disks with mycelia were transferred to MEA culture media without cryoprotectant and kept at 28 ºC for 30 days. A. blazei strains respond differently to the cryopreservation method at -196 ºC by varying mycelial viability recovery. Cryopreservation with liquid nitrogen, using dimethyl sulfoxide as cryoprotectant, is not the most appropriate one for A. blazei preservation. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-12-21 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/14114 |
url |
https://seer.ufu.br/index.php/biosciencejournal/article/view/14114 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/14114/11129 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2012 Nelson Barros Colauto, Augusto Ferreira da Eira, Giani Andrea Linde https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2012 Nelson Barros Colauto, Augusto Ferreira da Eira, Giani Andrea Linde https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
Brazil; Contemporany |
dc.publisher.none.fl_str_mv |
EDUFU |
publisher.none.fl_str_mv |
EDUFU |
dc.source.none.fl_str_mv |
Bioscience Journal ; Vol. 28 No. 6 (2012): Nov./Dec.; 1034-1037 Bioscience Journal ; v. 28 n. 6 (2012): Nov./Dec.; 1034-1037 1981-3163 reponame:Bioscience journal (Online) instname:Universidade Federal de Uberlândia (UFU) instacron:UFU |
instname_str |
Universidade Federal de Uberlândia (UFU) |
instacron_str |
UFU |
institution |
UFU |
reponame_str |
Bioscience journal (Online) |
collection |
Bioscience journal (Online) |
repository.name.fl_str_mv |
Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU) |
repository.mail.fl_str_mv |
biosciencej@ufu.br|| |
_version_ |
1797069071951331328 |