Experimental study on hplc determination of astragaloside in qishen huoxue granule and its protective effect in septic rats
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Bioscience journal (Online) |
Texto Completo: | https://seer.ufu.br/index.php/biosciencejournal/article/view/29581 |
Resumo: | Objective: To determine the astragaloside content in Qishen Huoxue Granule (QHG) by HPLC and to investigate the protective effect of astragaloside in septic rats. Methods: Dikma DiamonsilTM C18 column was used. Mobile phase was methanol: acetonitrile (42:58) and detection was performed at 205 nm. Healthy rats were randomly divided into three groups, group A: sham operation (SO) group; group B: cecal ligation and puncture (CLP) group; and group C: CLP QHG treatment group. 72 h after model establishment, plasma tumor necrosis factor-α (TNF-α), plasma and peritoneal albumin concentrations, as well as mesenteric venular flow velocity in each group were measured and degree of intestinal mucosal injury was observed. Results: Average recovery was 100.7%, with a RSD of 2.20%. Sample concentration exhibited good linearity with peak area within a 0.01~0.2 mg/mL range. After CLP, plasma TNF-α concentration and albumin leakage in rats increased markedly; such increases were more significant in group B than group C (P<0.01). Changes in intestinal mucosal scores also presented the same trend (P<0.01). Mesenteric venular flow slowed down and plasma albumin concentration lowered; such decreases were more significant in group B than group C (P<0.05). Conclusion: QHG can reduce the inflammatory response and intestinal mucosal injury in rats with CLP-induced sepsis. |
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Experimental study on hplc determination of astragaloside in qishen huoxue granule and its protective effect in septic rats AstragalosideHPLCQishen Huoxue Granule Health SciencesObjective: To determine the astragaloside content in Qishen Huoxue Granule (QHG) by HPLC and to investigate the protective effect of astragaloside in septic rats. Methods: Dikma DiamonsilTM C18 column was used. Mobile phase was methanol: acetonitrile (42:58) and detection was performed at 205 nm. Healthy rats were randomly divided into three groups, group A: sham operation (SO) group; group B: cecal ligation and puncture (CLP) group; and group C: CLP QHG treatment group. 72 h after model establishment, plasma tumor necrosis factor-α (TNF-α), plasma and peritoneal albumin concentrations, as well as mesenteric venular flow velocity in each group were measured and degree of intestinal mucosal injury was observed. Results: Average recovery was 100.7%, with a RSD of 2.20%. Sample concentration exhibited good linearity with peak area within a 0.01~0.2 mg/mL range. After CLP, plasma TNF-α concentration and albumin leakage in rats increased markedly; such increases were more significant in group B than group C (P<0.01). Changes in intestinal mucosal scores also presented the same trend (P<0.01). Mesenteric venular flow slowed down and plasma albumin concentration lowered; such decreases were more significant in group B than group C (P<0.05). Conclusion: QHG can reduce the inflammatory response and intestinal mucosal injury in rats with CLP-induced sepsis.EDUFU2016-01-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/2958110.14393/BJ-v32n1a2016-29581Bioscience Journal ; Vol. 32 No. 1 (2016): Jan./Feb.; 255-261Bioscience Journal ; v. 32 n. 1 (2016): Jan./Feb.; 255-2611981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/29581/17774China; ContemporaryCopyright (c) 2016 Xiaoqi Sun, Hong Zhang, Wenhua Zou, Ying Chenhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSun, XiaoqiZhang, HongZou, WenhuaChen, Ying2022-05-24T18:04:11Zoai:ojs.www.seer.ufu.br:article/29581Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2022-05-24T18:04:11Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false |
dc.title.none.fl_str_mv |
Experimental study on hplc determination of astragaloside in qishen huoxue granule and its protective effect in septic rats |
title |
Experimental study on hplc determination of astragaloside in qishen huoxue granule and its protective effect in septic rats |
spellingShingle |
Experimental study on hplc determination of astragaloside in qishen huoxue granule and its protective effect in septic rats Sun, Xiaoqi Astragaloside HPLC Qishen Huoxue Granule Health Sciences |
title_short |
Experimental study on hplc determination of astragaloside in qishen huoxue granule and its protective effect in septic rats |
title_full |
Experimental study on hplc determination of astragaloside in qishen huoxue granule and its protective effect in septic rats |
title_fullStr |
Experimental study on hplc determination of astragaloside in qishen huoxue granule and its protective effect in septic rats |
title_full_unstemmed |
Experimental study on hplc determination of astragaloside in qishen huoxue granule and its protective effect in septic rats |
title_sort |
Experimental study on hplc determination of astragaloside in qishen huoxue granule and its protective effect in septic rats |
author |
Sun, Xiaoqi |
author_facet |
Sun, Xiaoqi Zhang, Hong Zou, Wenhua Chen, Ying |
author_role |
author |
author2 |
Zhang, Hong Zou, Wenhua Chen, Ying |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Sun, Xiaoqi Zhang, Hong Zou, Wenhua Chen, Ying |
dc.subject.por.fl_str_mv |
Astragaloside HPLC Qishen Huoxue Granule Health Sciences |
topic |
Astragaloside HPLC Qishen Huoxue Granule Health Sciences |
description |
Objective: To determine the astragaloside content in Qishen Huoxue Granule (QHG) by HPLC and to investigate the protective effect of astragaloside in septic rats. Methods: Dikma DiamonsilTM C18 column was used. Mobile phase was methanol: acetonitrile (42:58) and detection was performed at 205 nm. Healthy rats were randomly divided into three groups, group A: sham operation (SO) group; group B: cecal ligation and puncture (CLP) group; and group C: CLP QHG treatment group. 72 h after model establishment, plasma tumor necrosis factor-α (TNF-α), plasma and peritoneal albumin concentrations, as well as mesenteric venular flow velocity in each group were measured and degree of intestinal mucosal injury was observed. Results: Average recovery was 100.7%, with a RSD of 2.20%. Sample concentration exhibited good linearity with peak area within a 0.01~0.2 mg/mL range. After CLP, plasma TNF-α concentration and albumin leakage in rats increased markedly; such increases were more significant in group B than group C (P<0.01). Changes in intestinal mucosal scores also presented the same trend (P<0.01). Mesenteric venular flow slowed down and plasma albumin concentration lowered; such decreases were more significant in group B than group C (P<0.05). Conclusion: QHG can reduce the inflammatory response and intestinal mucosal injury in rats with CLP-induced sepsis. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-01-20 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/29581 10.14393/BJ-v32n1a2016-29581 |
url |
https://seer.ufu.br/index.php/biosciencejournal/article/view/29581 |
identifier_str_mv |
10.14393/BJ-v32n1a2016-29581 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/29581/17774 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2016 Xiaoqi Sun, Hong Zhang, Wenhua Zou, Ying Chen https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2016 Xiaoqi Sun, Hong Zhang, Wenhua Zou, Ying Chen https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
China; Contemporary |
dc.publisher.none.fl_str_mv |
EDUFU |
publisher.none.fl_str_mv |
EDUFU |
dc.source.none.fl_str_mv |
Bioscience Journal ; Vol. 32 No. 1 (2016): Jan./Feb.; 255-261 Bioscience Journal ; v. 32 n. 1 (2016): Jan./Feb.; 255-261 1981-3163 reponame:Bioscience journal (Online) instname:Universidade Federal de Uberlândia (UFU) instacron:UFU |
instname_str |
Universidade Federal de Uberlândia (UFU) |
instacron_str |
UFU |
institution |
UFU |
reponame_str |
Bioscience journal (Online) |
collection |
Bioscience journal (Online) |
repository.name.fl_str_mv |
Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU) |
repository.mail.fl_str_mv |
biosciencej@ufu.br|| |
_version_ |
1797069075791216640 |