Clonal cleaning and in vitro multiplication of Angelonia integerrima sprengel
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Bioscience journal (Online) |
Texto Completo: | https://seer.ufu.br/index.php/biosciencejournal/article/view/39442 |
Resumo: | The objective of this study is to test stem apex sizes in the in vitro establishing of Angelonia integerrima in order to obtain explants without by fungi and bacteria contamination for further multiplications. The treatments consisted of different stem apex sizes (1.0, 3.0, 5.0, 7.0, 9.0 and 11.0 mm). At 45 and 90 days of cultivation, a count of contaminated explants and a count of shoots per explant formed were performed. In a second experiment, explants were cultivated in a medium containing different concentrations of benzylaminopurine (BAP) (0.0, 0.05, 0.10, 0.15 and 0.20 mg L-1). After 56 days of cultivation, the following variables were evaluated: shoot length, shoot fresh mass and number of shoots. During the explant establishment phase (45 days), only stem apexes with 1.0 mm in size were not contaminated. However, in the second subculture (at 90 days), only shoots from initial explants, with 7 mm in size or larger, were contaminated. Regarding multiplication, the presence of BAP showed a positive linear behavior for all variables. It is possible to obtain A. integerrima seedlings free of contamination in vitro by fungi and bacteria, using initial explants less than or equal to 5 mm. IBA provided a linear increment for the multiplication of this species. |
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Clonal cleaning and in vitro multiplication of Angelonia integerrima sprengel Limpeza clonal e multiplicação in vitro de Angelonia integerrima Sprengel Pampa biomestem apexnative ornamentalPlantaginaceaeAgricultural SciencesThe objective of this study is to test stem apex sizes in the in vitro establishing of Angelonia integerrima in order to obtain explants without by fungi and bacteria contamination for further multiplications. The treatments consisted of different stem apex sizes (1.0, 3.0, 5.0, 7.0, 9.0 and 11.0 mm). At 45 and 90 days of cultivation, a count of contaminated explants and a count of shoots per explant formed were performed. In a second experiment, explants were cultivated in a medium containing different concentrations of benzylaminopurine (BAP) (0.0, 0.05, 0.10, 0.15 and 0.20 mg L-1). After 56 days of cultivation, the following variables were evaluated: shoot length, shoot fresh mass and number of shoots. During the explant establishment phase (45 days), only stem apexes with 1.0 mm in size were not contaminated. However, in the second subculture (at 90 days), only shoots from initial explants, with 7 mm in size or larger, were contaminated. Regarding multiplication, the presence of BAP showed a positive linear behavior for all variables. It is possible to obtain A. integerrima seedlings free of contamination in vitro by fungi and bacteria, using initial explants less than or equal to 5 mm. IBA provided a linear increment for the multiplication of this species.O objetivo do trabalho foi testar tamanhos de ápices caulinares no estabelecimento in vitro de Angelonia integerrima, a fim de obter explantes sem contaminações por fungos e bactérias para posterior multiplicação. Os tratamentos consistiram de tamanhos de ápices caulinares: 1,0; 3,0; 5,0; 7,0; 9,0 e 11,0 mm. Após 45 e 90 dias de cultivo foi realizada a contagem de explantes contaminados e o número de brotos formados por explante. Em um segundo experimento, explantes foram cultivados em meio contendo diferentes concentrações de benzilaminopurina (BAP): 0,0; 0,05; 0,10; 0,15 e 0,20 mg L-1. Após 56 dias de cultivo foram avaliados: comprimento e massa fresca da parte aérea e número de brotos. Durante a fase de estabelecimento (45 dias), somente explantes com 1,0 mm não apresentaram contaminação, já no segundo subcultivo (aos 90 dias) somente brotações oriundas de explantes com tamanho inicial igual ou superior a 7 mm apresentaram contaminação. Com relação à multiplicação, a presença de BAP apresentou comportamento linear positivo para todas as variáveis analisadas. É possível obter mudas de A. integerrima livres de contaminações in vitro por fungos e bactérias, utilizando explantes iniciais menores ou iguais a 5 mm. O BAP proporcionou incremento linear para a multiplicação da espécie.EDUFU2018-10-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/3944210.14393/BJ-v34n5a2018-39442Bioscience Journal ; Vol. 34 No. 5 (2018): Sept./Oct.; 1258-1263Bioscience Journal ; v. 34 n. 5 (2018): Sept./Oct.; 1258-12631981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/39442/24214Brazil; ContemporaryCopyright (c) 2018 Mara Cíntia Winhelmann, Marília Tedesco, Priscila Paris, Eduarda Demari Avrella, Claudimar Sidnei Fior, Gilmar Schaferhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessWinhelmann, Mara CíntiaTedesco, MaríliaParis, PriscilaAvrella, Eduarda DemariFior, Claudimar SidneiSchafer, Gilmar2022-02-10T12:10:10Zoai:ojs.www.seer.ufu.br:article/39442Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2022-02-10T12:10:10Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false |
dc.title.none.fl_str_mv |
Clonal cleaning and in vitro multiplication of Angelonia integerrima sprengel Limpeza clonal e multiplicação in vitro de Angelonia integerrima Sprengel |
title |
Clonal cleaning and in vitro multiplication of Angelonia integerrima sprengel |
spellingShingle |
Clonal cleaning and in vitro multiplication of Angelonia integerrima sprengel Winhelmann, Mara Cíntia Pampa biome stem apex native ornamental Plantaginaceae Agricultural Sciences |
title_short |
Clonal cleaning and in vitro multiplication of Angelonia integerrima sprengel |
title_full |
Clonal cleaning and in vitro multiplication of Angelonia integerrima sprengel |
title_fullStr |
Clonal cleaning and in vitro multiplication of Angelonia integerrima sprengel |
title_full_unstemmed |
Clonal cleaning and in vitro multiplication of Angelonia integerrima sprengel |
title_sort |
Clonal cleaning and in vitro multiplication of Angelonia integerrima sprengel |
author |
Winhelmann, Mara Cíntia |
author_facet |
Winhelmann, Mara Cíntia Tedesco, Marília Paris, Priscila Avrella, Eduarda Demari Fior, Claudimar Sidnei Schafer, Gilmar |
author_role |
author |
author2 |
Tedesco, Marília Paris, Priscila Avrella, Eduarda Demari Fior, Claudimar Sidnei Schafer, Gilmar |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Winhelmann, Mara Cíntia Tedesco, Marília Paris, Priscila Avrella, Eduarda Demari Fior, Claudimar Sidnei Schafer, Gilmar |
dc.subject.por.fl_str_mv |
Pampa biome stem apex native ornamental Plantaginaceae Agricultural Sciences |
topic |
Pampa biome stem apex native ornamental Plantaginaceae Agricultural Sciences |
description |
The objective of this study is to test stem apex sizes in the in vitro establishing of Angelonia integerrima in order to obtain explants without by fungi and bacteria contamination for further multiplications. The treatments consisted of different stem apex sizes (1.0, 3.0, 5.0, 7.0, 9.0 and 11.0 mm). At 45 and 90 days of cultivation, a count of contaminated explants and a count of shoots per explant formed were performed. In a second experiment, explants were cultivated in a medium containing different concentrations of benzylaminopurine (BAP) (0.0, 0.05, 0.10, 0.15 and 0.20 mg L-1). After 56 days of cultivation, the following variables were evaluated: shoot length, shoot fresh mass and number of shoots. During the explant establishment phase (45 days), only stem apexes with 1.0 mm in size were not contaminated. However, in the second subculture (at 90 days), only shoots from initial explants, with 7 mm in size or larger, were contaminated. Regarding multiplication, the presence of BAP showed a positive linear behavior for all variables. It is possible to obtain A. integerrima seedlings free of contamination in vitro by fungi and bacteria, using initial explants less than or equal to 5 mm. IBA provided a linear increment for the multiplication of this species. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-10-11 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/39442 10.14393/BJ-v34n5a2018-39442 |
url |
https://seer.ufu.br/index.php/biosciencejournal/article/view/39442 |
identifier_str_mv |
10.14393/BJ-v34n5a2018-39442 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/39442/24214 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
Brazil; Contemporary |
dc.publisher.none.fl_str_mv |
EDUFU |
publisher.none.fl_str_mv |
EDUFU |
dc.source.none.fl_str_mv |
Bioscience Journal ; Vol. 34 No. 5 (2018): Sept./Oct.; 1258-1263 Bioscience Journal ; v. 34 n. 5 (2018): Sept./Oct.; 1258-1263 1981-3163 reponame:Bioscience journal (Online) instname:Universidade Federal de Uberlândia (UFU) instacron:UFU |
instname_str |
Universidade Federal de Uberlândia (UFU) |
instacron_str |
UFU |
institution |
UFU |
reponame_str |
Bioscience journal (Online) |
collection |
Bioscience journal (Online) |
repository.name.fl_str_mv |
Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU) |
repository.mail.fl_str_mv |
biosciencej@ufu.br|| |
_version_ |
1797069078407413760 |