Clonal cleaning and in vitro multiplication of Angelonia integerrima sprengel

Detalhes bibliográficos
Autor(a) principal: Winhelmann, Mara Cíntia
Data de Publicação: 2018
Outros Autores: Tedesco, Marília, Paris, Priscila, Avrella, Eduarda Demari, Fior, Claudimar Sidnei, Schafer, Gilmar
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Bioscience journal (Online)
Texto Completo: https://seer.ufu.br/index.php/biosciencejournal/article/view/39442
Resumo: The objective of this study is to test stem apex sizes in the in vitro establishing of Angelonia integerrima in order to obtain explants without by fungi and bacteria contamination for further multiplications. The treatments consisted of different stem apex sizes (1.0, 3.0, 5.0, 7.0, 9.0 and 11.0 mm). At 45 and 90 days of cultivation, a count of contaminated explants and a count of shoots per explant formed were performed. In a second experiment, explants were cultivated in a medium containing different concentrations of benzylaminopurine (BAP) (0.0, 0.05, 0.10, 0.15 and 0.20 mg L-1). After 56 days of cultivation, the following variables were evaluated: shoot length, shoot fresh mass and number of shoots. During the explant establishment phase (45 days), only stem apexes with 1.0 mm in size were not contaminated. However, in the second subculture (at 90 days), only shoots from initial explants, with 7 mm in size or larger, were contaminated. Regarding multiplication, the presence of BAP showed a positive linear behavior for all variables. It is possible to obtain A. integerrima seedlings free of contamination in vitro by fungi and bacteria, using initial explants less than or equal to 5 mm. IBA provided a linear increment for the multiplication of this species.
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spelling Clonal cleaning and in vitro multiplication of Angelonia integerrima sprengel Limpeza clonal e multiplicação in vitro de Angelonia integerrima Sprengel Pampa biomestem apexnative ornamentalPlantaginaceaeAgricultural SciencesThe objective of this study is to test stem apex sizes in the in vitro establishing of Angelonia integerrima in order to obtain explants without by fungi and bacteria contamination for further multiplications. The treatments consisted of different stem apex sizes (1.0, 3.0, 5.0, 7.0, 9.0 and 11.0 mm). At 45 and 90 days of cultivation, a count of contaminated explants and a count of shoots per explant formed were performed. In a second experiment, explants were cultivated in a medium containing different concentrations of benzylaminopurine (BAP) (0.0, 0.05, 0.10, 0.15 and 0.20 mg L-1). After 56 days of cultivation, the following variables were evaluated: shoot length, shoot fresh mass and number of shoots. During the explant establishment phase (45 days), only stem apexes with 1.0 mm in size were not contaminated. However, in the second subculture (at 90 days), only shoots from initial explants, with 7 mm in size or larger, were contaminated. Regarding multiplication, the presence of BAP showed a positive linear behavior for all variables. It is possible to obtain A. integerrima seedlings free of contamination in vitro by fungi and bacteria, using initial explants less than or equal to 5 mm. IBA provided a linear increment for the multiplication of this species.O objetivo do trabalho foi testar tamanhos de ápices caulinares no estabelecimento in vitro de Angelonia integerrima, a fim de obter explantes sem contaminações por fungos e bactérias para posterior multiplicação. Os tratamentos consistiram de tamanhos de ápices caulinares: 1,0; 3,0; 5,0; 7,0; 9,0 e 11,0 mm. Após 45 e 90 dias de cultivo foi realizada a contagem de explantes contaminados e o número de brotos formados por explante. Em um segundo experimento, explantes foram cultivados em meio contendo diferentes concentrações de benzilaminopurina (BAP): 0,0; 0,05; 0,10; 0,15 e 0,20 mg L-1. Após 56 dias de cultivo foram avaliados: comprimento e massa fresca da parte aérea e número de brotos. Durante a fase de estabelecimento (45 dias), somente explantes com 1,0 mm não apresentaram contaminação, já no segundo subcultivo (aos 90 dias) somente brotações oriundas de explantes com tamanho inicial igual ou superior a 7 mm apresentaram contaminação. Com relação à multiplicação, a presença de BAP apresentou comportamento linear positivo para todas as variáveis analisadas. É possível obter mudas de A. integerrima livres de contaminações in vitro por fungos e bactérias, utilizando explantes iniciais menores ou iguais a 5 mm. O BAP proporcionou incremento linear para a multiplicação da espécie.EDUFU2018-10-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/3944210.14393/BJ-v34n5a2018-39442Bioscience Journal ; Vol. 34 No. 5 (2018): Sept./Oct.; 1258-1263Bioscience Journal ; v. 34 n. 5 (2018): Sept./Oct.; 1258-12631981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/39442/24214Brazil; ContemporaryCopyright (c) 2018 Mara Cíntia Winhelmann, Marília Tedesco, Priscila Paris, Eduarda Demari Avrella, Claudimar Sidnei Fior, Gilmar Schaferhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessWinhelmann, Mara CíntiaTedesco, MaríliaParis, PriscilaAvrella, Eduarda DemariFior, Claudimar SidneiSchafer, Gilmar2022-02-10T12:10:10Zoai:ojs.www.seer.ufu.br:article/39442Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2022-02-10T12:10:10Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false
dc.title.none.fl_str_mv Clonal cleaning and in vitro multiplication of Angelonia integerrima sprengel
Limpeza clonal e multiplicação in vitro de Angelonia integerrima Sprengel
title Clonal cleaning and in vitro multiplication of Angelonia integerrima sprengel
spellingShingle Clonal cleaning and in vitro multiplication of Angelonia integerrima sprengel
Winhelmann, Mara Cíntia
Pampa biome
stem apex
native ornamental
Plantaginaceae
Agricultural Sciences
title_short Clonal cleaning and in vitro multiplication of Angelonia integerrima sprengel
title_full Clonal cleaning and in vitro multiplication of Angelonia integerrima sprengel
title_fullStr Clonal cleaning and in vitro multiplication of Angelonia integerrima sprengel
title_full_unstemmed Clonal cleaning and in vitro multiplication of Angelonia integerrima sprengel
title_sort Clonal cleaning and in vitro multiplication of Angelonia integerrima sprengel
author Winhelmann, Mara Cíntia
author_facet Winhelmann, Mara Cíntia
Tedesco, Marília
Paris, Priscila
Avrella, Eduarda Demari
Fior, Claudimar Sidnei
Schafer, Gilmar
author_role author
author2 Tedesco, Marília
Paris, Priscila
Avrella, Eduarda Demari
Fior, Claudimar Sidnei
Schafer, Gilmar
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Winhelmann, Mara Cíntia
Tedesco, Marília
Paris, Priscila
Avrella, Eduarda Demari
Fior, Claudimar Sidnei
Schafer, Gilmar
dc.subject.por.fl_str_mv Pampa biome
stem apex
native ornamental
Plantaginaceae
Agricultural Sciences
topic Pampa biome
stem apex
native ornamental
Plantaginaceae
Agricultural Sciences
description The objective of this study is to test stem apex sizes in the in vitro establishing of Angelonia integerrima in order to obtain explants without by fungi and bacteria contamination for further multiplications. The treatments consisted of different stem apex sizes (1.0, 3.0, 5.0, 7.0, 9.0 and 11.0 mm). At 45 and 90 days of cultivation, a count of contaminated explants and a count of shoots per explant formed were performed. In a second experiment, explants were cultivated in a medium containing different concentrations of benzylaminopurine (BAP) (0.0, 0.05, 0.10, 0.15 and 0.20 mg L-1). After 56 days of cultivation, the following variables were evaluated: shoot length, shoot fresh mass and number of shoots. During the explant establishment phase (45 days), only stem apexes with 1.0 mm in size were not contaminated. However, in the second subculture (at 90 days), only shoots from initial explants, with 7 mm in size or larger, were contaminated. Regarding multiplication, the presence of BAP showed a positive linear behavior for all variables. It is possible to obtain A. integerrima seedlings free of contamination in vitro by fungi and bacteria, using initial explants less than or equal to 5 mm. IBA provided a linear increment for the multiplication of this species.
publishDate 2018
dc.date.none.fl_str_mv 2018-10-11
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.ufu.br/index.php/biosciencejournal/article/view/39442
10.14393/BJ-v34n5a2018-39442
url https://seer.ufu.br/index.php/biosciencejournal/article/view/39442
identifier_str_mv 10.14393/BJ-v34n5a2018-39442
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.ufu.br/index.php/biosciencejournal/article/view/39442/24214
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Brazil; Contemporary
dc.publisher.none.fl_str_mv EDUFU
publisher.none.fl_str_mv EDUFU
dc.source.none.fl_str_mv Bioscience Journal ; Vol. 34 No. 5 (2018): Sept./Oct.; 1258-1263
Bioscience Journal ; v. 34 n. 5 (2018): Sept./Oct.; 1258-1263
1981-3163
reponame:Bioscience journal (Online)
instname:Universidade Federal de Uberlândia (UFU)
instacron:UFU
instname_str Universidade Federal de Uberlândia (UFU)
instacron_str UFU
institution UFU
reponame_str Bioscience journal (Online)
collection Bioscience journal (Online)
repository.name.fl_str_mv Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)
repository.mail.fl_str_mv biosciencej@ufu.br||
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