Substract and temperature effect on xylanase production by aspergillus fumigatus using low cost agricultural wastes

Detalhes bibliográficos
Autor(a) principal: Sousa Gomes, Arthur Filipe
Data de Publicação: 2016
Outros Autores: Lamanes dos Santos, Bruna Silveira, Franciscon, Emanuele Giuliani, Baffi, Milla Alves
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Bioscience journal (Online)
Texto Completo: https://seer.ufu.br/index.php/biosciencejournal/article/view/32935
Resumo: This study reports the optimization of xylanase production under solid state fermentation (SSF) by a thermotolerant Aspergillus fumigatus strain (SCB4) isolated from sugarcane bagasse piles of Brazilian Cerrado. Different combinations of low-cost agricultural byproducts in SSF were evaluated: sugarcane bagasse and wheat bran (1:1), sugarcane bagasse and corn straw (1:1) and only sugarcane bagasse. The enzyme biosynthesis by SSF was carried out at different temperatures (40, 45, 50 and 55 oC). The maximum levels of xylanase activity were obtained after 24 h at 45 °C using a culture medium containing sugarcane bagasse and wheat bran (1:1). Under optimal conditions, the fungal culture produced 574 U g-1of xylanase (units/g of dry substrate). The crude enzyme showed optimal activity at 60 °C and pH 4.5. It exhibited thermostability up to 55 °C, wide range of pH stability and tolerance to ethanol, xylose and glucose. The physicochemical properties shown by this enzyme are appropriate for its application in hydrolysis of lignocellulosic residues for ethanol production and other bioproducts. 
id UFU-14_e81f23f60af6a66f9259317d255021eb
oai_identifier_str oai:ojs.www.seer.ufu.br:article/32935
network_acronym_str UFU-14
network_name_str Bioscience journal (Online)
repository_id_str
spelling Substract and temperature effect on xylanase production by aspergillus fumigatus using low cost agricultural wastes sugarcane bagassewheat breancorn strawxylanaseAspergillus fumigatusAgricultural SciencesThis study reports the optimization of xylanase production under solid state fermentation (SSF) by a thermotolerant Aspergillus fumigatus strain (SCB4) isolated from sugarcane bagasse piles of Brazilian Cerrado. Different combinations of low-cost agricultural byproducts in SSF were evaluated: sugarcane bagasse and wheat bran (1:1), sugarcane bagasse and corn straw (1:1) and only sugarcane bagasse. The enzyme biosynthesis by SSF was carried out at different temperatures (40, 45, 50 and 55 oC). The maximum levels of xylanase activity were obtained after 24 h at 45 °C using a culture medium containing sugarcane bagasse and wheat bran (1:1). Under optimal conditions, the fungal culture produced 574 U g-1of xylanase (units/g of dry substrate). The crude enzyme showed optimal activity at 60 °C and pH 4.5. It exhibited thermostability up to 55 °C, wide range of pH stability and tolerance to ethanol, xylose and glucose. The physicochemical properties shown by this enzyme are appropriate for its application in hydrolysis of lignocellulosic residues for ethanol production and other bioproducts. EDUFU2016-07-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/3293510.14393/BJ-v32n4a2016-32935Bioscience Journal ; Vol. 32 No. 4 (2016): July/Aug.; 915-921Bioscience Journal ; v. 32 n. 4 (2016): July/Aug.; 915-9211981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/32935/18527Brazil; ContemporaryCopyright (c) 2016 Arthur Filipe Sousa Gomes, Bruna Silveira Lamanes dos Santos, Emanuele Giuliani Franciscon, Milla Alves Baffihttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSousa Gomes, Arthur FilipeLamanes dos Santos, Bruna SilveiraFranciscon, Emanuele GiulianiBaffi, Milla Alves2022-02-22T02:38:13Zoai:ojs.www.seer.ufu.br:article/32935Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2022-02-22T02:38:13Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false
dc.title.none.fl_str_mv Substract and temperature effect on xylanase production by aspergillus fumigatus using low cost agricultural wastes
title Substract and temperature effect on xylanase production by aspergillus fumigatus using low cost agricultural wastes
spellingShingle Substract and temperature effect on xylanase production by aspergillus fumigatus using low cost agricultural wastes
Sousa Gomes, Arthur Filipe
sugarcane bagasse
wheat brean
corn straw
xylanase
Aspergillus fumigatus
Agricultural Sciences
title_short Substract and temperature effect on xylanase production by aspergillus fumigatus using low cost agricultural wastes
title_full Substract and temperature effect on xylanase production by aspergillus fumigatus using low cost agricultural wastes
title_fullStr Substract and temperature effect on xylanase production by aspergillus fumigatus using low cost agricultural wastes
title_full_unstemmed Substract and temperature effect on xylanase production by aspergillus fumigatus using low cost agricultural wastes
title_sort Substract and temperature effect on xylanase production by aspergillus fumigatus using low cost agricultural wastes
author Sousa Gomes, Arthur Filipe
author_facet Sousa Gomes, Arthur Filipe
Lamanes dos Santos, Bruna Silveira
Franciscon, Emanuele Giuliani
Baffi, Milla Alves
author_role author
author2 Lamanes dos Santos, Bruna Silveira
Franciscon, Emanuele Giuliani
Baffi, Milla Alves
author2_role author
author
author
dc.contributor.author.fl_str_mv Sousa Gomes, Arthur Filipe
Lamanes dos Santos, Bruna Silveira
Franciscon, Emanuele Giuliani
Baffi, Milla Alves
dc.subject.por.fl_str_mv sugarcane bagasse
wheat brean
corn straw
xylanase
Aspergillus fumigatus
Agricultural Sciences
topic sugarcane bagasse
wheat brean
corn straw
xylanase
Aspergillus fumigatus
Agricultural Sciences
description This study reports the optimization of xylanase production under solid state fermentation (SSF) by a thermotolerant Aspergillus fumigatus strain (SCB4) isolated from sugarcane bagasse piles of Brazilian Cerrado. Different combinations of low-cost agricultural byproducts in SSF were evaluated: sugarcane bagasse and wheat bran (1:1), sugarcane bagasse and corn straw (1:1) and only sugarcane bagasse. The enzyme biosynthesis by SSF was carried out at different temperatures (40, 45, 50 and 55 oC). The maximum levels of xylanase activity were obtained after 24 h at 45 °C using a culture medium containing sugarcane bagasse and wheat bran (1:1). Under optimal conditions, the fungal culture produced 574 U g-1of xylanase (units/g of dry substrate). The crude enzyme showed optimal activity at 60 °C and pH 4.5. It exhibited thermostability up to 55 °C, wide range of pH stability and tolerance to ethanol, xylose and glucose. The physicochemical properties shown by this enzyme are appropriate for its application in hydrolysis of lignocellulosic residues for ethanol production and other bioproducts. 
publishDate 2016
dc.date.none.fl_str_mv 2016-07-08
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.ufu.br/index.php/biosciencejournal/article/view/32935
10.14393/BJ-v32n4a2016-32935
url https://seer.ufu.br/index.php/biosciencejournal/article/view/32935
identifier_str_mv 10.14393/BJ-v32n4a2016-32935
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.ufu.br/index.php/biosciencejournal/article/view/32935/18527
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Brazil; Contemporary
dc.publisher.none.fl_str_mv EDUFU
publisher.none.fl_str_mv EDUFU
dc.source.none.fl_str_mv Bioscience Journal ; Vol. 32 No. 4 (2016): July/Aug.; 915-921
Bioscience Journal ; v. 32 n. 4 (2016): July/Aug.; 915-921
1981-3163
reponame:Bioscience journal (Online)
instname:Universidade Federal de Uberlândia (UFU)
instacron:UFU
instname_str Universidade Federal de Uberlândia (UFU)
instacron_str UFU
institution UFU
reponame_str Bioscience journal (Online)
collection Bioscience journal (Online)
repository.name.fl_str_mv Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)
repository.mail.fl_str_mv biosciencej@ufu.br||
_version_ 1797069076400439296

Registros relacionados