Carcinogênese da próstata de camundongos: papel do receptor tipo I do TNF-α na incidência tumoral
Autor(a) principal: | |
---|---|
Data de Publicação: | 2015 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFU |
Texto Completo: | https://repositorio.ufu.br/handle/123456789/12415 https://doi.org/10.14393/ufu.di.2015.444 |
Resumo: | TNF-α is a key cytokine involved in process of carcinogenesis, including prostate cacrinogenesis, being TNFR-1 the responsible for the majority of its answers. This receptor triggers two opposite pathways: cell death or cell survival. So, due to this paradoxal role, a TNFR-1 knockout model is interesting in order to clarify the real effect of TNF in prostate cancer. Thus, this investigation had as a general propose to evaluate the role of TNF signalling pathway in prostate carcinogenesis by chemical induction in mice. Therefore, C57bl/6 wild type (WT) and TNFR-1 knockout mice (KO) were treated with mineral oil or MNU in association with testosterone (MNU+T) during 6 months. After this period of induction, prostate samples were processed for histological and biochemical analysis. It was observed that the treatment with MNU+T led to development of benign and malign lesions in both WT e KO animals. However, the incidence of malign lesions was significantly higher in the former, indicating the involvement of TNFR-1 with cellular survival and proliferation pathaway. These data were supported by proliferative profile analysis, which showed that proloferation was significantly lower in KO, even after carcinogenesis. Moreover, knockout animals had smaller prostate amounts of mTOR after treatment with MNU+T in comparison to WT, suggesting the potential of TNFR-1 in activating this proliferative pathway. It was also observed a decrease in AR quantities in prostate after carcinogenesis in both KO and WT. Since AR expression may be regulated as a result of PI3K/AKT/mTOR pathway activation, it is suggested that the decrease of AR in KO may have occurred as a result of smaller quantities of mTOR in these animals. Moreover, it s suggested that TNF-α /TNFR-1 has key role in MMP2 expression, reducing the content of this enzyme in its absence, and that the decrease of MMP2 may be responsible to the accumulation of fibronectin in KO. Finally, it was observed a similar increase in apoptosis in WT and KO group after carcinogenesis, showing clearly that TNFR-1 in this model was not involved in the induction of cell death. Therefore, it is concluded that the cytokine TNF-α, through its receptor TNFR-1 promoted cell proliferation and cell survival by activation of the AKT/mTOR pathway, which implicates its role in prostate carcinogenesis in this experimental model. |
id |
UFU_05ac9270380886d3f46a2f21db0696d0 |
---|---|
oai_identifier_str |
oai:repositorio.ufu.br:123456789/12415 |
network_acronym_str |
UFU |
network_name_str |
Repositório Institucional da UFU |
repository_id_str |
|
spelling |
Carcinogênese da próstata de camundongos: papel do receptor tipo I do TNF-α na incidência tumoralTNFR-1Carcinogênese da próstataAKT/mTORPróstata - CâncerCitocinasProstate carcinogenesisCNPQ::CIENCIAS BIOLOGICAS::MORFOLOGIA::CITOLOGIA E BIOLOGIA CELULARTNF-α is a key cytokine involved in process of carcinogenesis, including prostate cacrinogenesis, being TNFR-1 the responsible for the majority of its answers. This receptor triggers two opposite pathways: cell death or cell survival. So, due to this paradoxal role, a TNFR-1 knockout model is interesting in order to clarify the real effect of TNF in prostate cancer. Thus, this investigation had as a general propose to evaluate the role of TNF signalling pathway in prostate carcinogenesis by chemical induction in mice. Therefore, C57bl/6 wild type (WT) and TNFR-1 knockout mice (KO) were treated with mineral oil or MNU in association with testosterone (MNU+T) during 6 months. After this period of induction, prostate samples were processed for histological and biochemical analysis. It was observed that the treatment with MNU+T led to development of benign and malign lesions in both WT e KO animals. However, the incidence of malign lesions was significantly higher in the former, indicating the involvement of TNFR-1 with cellular survival and proliferation pathaway. These data were supported by proliferative profile analysis, which showed that proloferation was significantly lower in KO, even after carcinogenesis. Moreover, knockout animals had smaller prostate amounts of mTOR after treatment with MNU+T in comparison to WT, suggesting the potential of TNFR-1 in activating this proliferative pathway. It was also observed a decrease in AR quantities in prostate after carcinogenesis in both KO and WT. Since AR expression may be regulated as a result of PI3K/AKT/mTOR pathway activation, it is suggested that the decrease of AR in KO may have occurred as a result of smaller quantities of mTOR in these animals. Moreover, it s suggested that TNF-α /TNFR-1 has key role in MMP2 expression, reducing the content of this enzyme in its absence, and that the decrease of MMP2 may be responsible to the accumulation of fibronectin in KO. Finally, it was observed a similar increase in apoptosis in WT and KO group after carcinogenesis, showing clearly that TNFR-1 in this model was not involved in the induction of cell death. Therefore, it is concluded that the cytokine TNF-α, through its receptor TNFR-1 promoted cell proliferation and cell survival by activation of the AKT/mTOR pathway, which implicates its role in prostate carcinogenesis in this experimental model.Mestre em Biologia Celular e Estrutural AplicadasO TNF-α é uma das principais citocinas envolvidas com o processo de carcinogênese, inclusive da próstata, sendo o TNFR-1 o receptor responsável pela maioria das suas respostas. Esse receptor desencadeia duas vias opostas: morte ou sobrevivência celular. Assim, devido a esse seu papel paradoxal, um modelo de knockout de TNFR-1 é interessante para esclarecer de forma mais específica o real efeito do TNF no câncer de próstata. Dessa forma, esse estudo teve como objetivo geral avaliar o papel da via de sinalização TNF-α na carcinogênese através da indução química do câncer de próstata em camundongos. Para tanto, camundongos C57bl/6 selvagens (WT) e knockout (KO) para o receptor TNFR-1 foram tratados com óleo mineral ou MNU associado a testosterona (MNU+T) durante o período de 6 meses. Após esse período de indução, observou-se que o tratamento com MNU+T levou ao desenvolvimento de lesões benignas e malignas em ambos animais WT e KO. Entretanto, a incidência de lesões malignas foi significativamente maior no primeiro grupo, indicando o envolvimento do TNFR-1 com a via de sobrevivência, proliferação celular e, assim, com a carcinogênese prostática. Esses dados foram reforçados pelas análises do perfil proliferativo das células, os quais mostraram que a proliferação foi substancialmente menor em KO, mesmo após a carcinogênese. Além disso, os animais KO apresentaram menores quantidades de mTOR após o tratamento com MNU+T, ao contrário do observado em WT, evidenciando o potencial de TNFR-1 em ativar a via que leva à fosforilação dessa proteína. Observou-se ainda uma queda nas quantidades de AR após o período de tratamento com carcinógeno tanto nos animais KO quanto nos WT. Devido ao fator de a expressão desse receptor poder ser regulada como resultado da ativação da via PI3K/AKT/mTOR, sugere-se que a diminuição de AR possa ter ocorrido como consequência da menor quantidade de mTOR nesses animais. Sugere-se ainda que a via TNF-α /TNFR-1 tenha papel fundamental na expressão de MMP2, reduzindo seu conteúdo na sua ausência, e que a diminuição da mesma pode ter relação com o aumento de fibronectina em KO. Por fim, foi observado um aumento da apoptose de maneira igual no grupo WT e em KO após o tratamento com MNU+T, deixando evidente que TNFR-1, nesse modelo, não teve envolvimento com a indução da morte celular. Portanto, conclui-se que a citocina TNF-α, através de seu receptor TNFR-1, promoveu proliferação e sobrevivência celular por meio da ativação da via AKT/mTOR, o que comprova seu papel na carcinogênese da próstata nesse modelo experimental.Universidade Federal de UberlândiaBRPrograma de Pós-graduação em Biologia Celular e Estrutural AplicadasCiências BiomédicasUFURibeiro, Daniele Lisboahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4773409A6Campos, Silvana Gisele Pegorin dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4707117D6Taboga, Sebastião Robertohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785629H9Galheigo, Maria Raquel Unterkircher2016-06-22T18:31:54Z2015-12-222016-06-22T18:31:54Z2015-08-28info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfGALHEIGO, Maria Raquel Unterkircher. Carcinogênese da próstata de camundongos: papel do receptor tipo I do TNF-α na incidência tumoral. 2015. 57 f. Dissertação (Mestrado em Ciências Biomédicas) - Universidade Federal de Uberlândia, Uberlândia, 2015. DOI https://doi.org/10.14393/ufu.di.2015.444https://repositorio.ufu.br/handle/123456789/12415https://doi.org/10.14393/ufu.di.2015.444porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFUinstname:Universidade Federal de Uberlândia (UFU)instacron:UFU2021-07-19T22:58:42Zoai:repositorio.ufu.br:123456789/12415Repositório InstitucionalONGhttp://repositorio.ufu.br/oai/requestdiinf@dirbi.ufu.bropendoar:2021-07-19T22:58:42Repositório Institucional da UFU - Universidade Federal de Uberlândia (UFU)false |
dc.title.none.fl_str_mv |
Carcinogênese da próstata de camundongos: papel do receptor tipo I do TNF-α na incidência tumoral |
title |
Carcinogênese da próstata de camundongos: papel do receptor tipo I do TNF-α na incidência tumoral |
spellingShingle |
Carcinogênese da próstata de camundongos: papel do receptor tipo I do TNF-α na incidência tumoral Galheigo, Maria Raquel Unterkircher TNFR-1 Carcinogênese da próstata AKT/mTOR Próstata - Câncer Citocinas Prostate carcinogenesis CNPQ::CIENCIAS BIOLOGICAS::MORFOLOGIA::CITOLOGIA E BIOLOGIA CELULAR |
title_short |
Carcinogênese da próstata de camundongos: papel do receptor tipo I do TNF-α na incidência tumoral |
title_full |
Carcinogênese da próstata de camundongos: papel do receptor tipo I do TNF-α na incidência tumoral |
title_fullStr |
Carcinogênese da próstata de camundongos: papel do receptor tipo I do TNF-α na incidência tumoral |
title_full_unstemmed |
Carcinogênese da próstata de camundongos: papel do receptor tipo I do TNF-α na incidência tumoral |
title_sort |
Carcinogênese da próstata de camundongos: papel do receptor tipo I do TNF-α na incidência tumoral |
author |
Galheigo, Maria Raquel Unterkircher |
author_facet |
Galheigo, Maria Raquel Unterkircher |
author_role |
author |
dc.contributor.none.fl_str_mv |
Ribeiro, Daniele Lisboa http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4773409A6 Campos, Silvana Gisele Pegorin de http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4707117D6 Taboga, Sebastião Roberto http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785629H9 |
dc.contributor.author.fl_str_mv |
Galheigo, Maria Raquel Unterkircher |
dc.subject.por.fl_str_mv |
TNFR-1 Carcinogênese da próstata AKT/mTOR Próstata - Câncer Citocinas Prostate carcinogenesis CNPQ::CIENCIAS BIOLOGICAS::MORFOLOGIA::CITOLOGIA E BIOLOGIA CELULAR |
topic |
TNFR-1 Carcinogênese da próstata AKT/mTOR Próstata - Câncer Citocinas Prostate carcinogenesis CNPQ::CIENCIAS BIOLOGICAS::MORFOLOGIA::CITOLOGIA E BIOLOGIA CELULAR |
description |
TNF-α is a key cytokine involved in process of carcinogenesis, including prostate cacrinogenesis, being TNFR-1 the responsible for the majority of its answers. This receptor triggers two opposite pathways: cell death or cell survival. So, due to this paradoxal role, a TNFR-1 knockout model is interesting in order to clarify the real effect of TNF in prostate cancer. Thus, this investigation had as a general propose to evaluate the role of TNF signalling pathway in prostate carcinogenesis by chemical induction in mice. Therefore, C57bl/6 wild type (WT) and TNFR-1 knockout mice (KO) were treated with mineral oil or MNU in association with testosterone (MNU+T) during 6 months. After this period of induction, prostate samples were processed for histological and biochemical analysis. It was observed that the treatment with MNU+T led to development of benign and malign lesions in both WT e KO animals. However, the incidence of malign lesions was significantly higher in the former, indicating the involvement of TNFR-1 with cellular survival and proliferation pathaway. These data were supported by proliferative profile analysis, which showed that proloferation was significantly lower in KO, even after carcinogenesis. Moreover, knockout animals had smaller prostate amounts of mTOR after treatment with MNU+T in comparison to WT, suggesting the potential of TNFR-1 in activating this proliferative pathway. It was also observed a decrease in AR quantities in prostate after carcinogenesis in both KO and WT. Since AR expression may be regulated as a result of PI3K/AKT/mTOR pathway activation, it is suggested that the decrease of AR in KO may have occurred as a result of smaller quantities of mTOR in these animals. Moreover, it s suggested that TNF-α /TNFR-1 has key role in MMP2 expression, reducing the content of this enzyme in its absence, and that the decrease of MMP2 may be responsible to the accumulation of fibronectin in KO. Finally, it was observed a similar increase in apoptosis in WT and KO group after carcinogenesis, showing clearly that TNFR-1 in this model was not involved in the induction of cell death. Therefore, it is concluded that the cytokine TNF-α, through its receptor TNFR-1 promoted cell proliferation and cell survival by activation of the AKT/mTOR pathway, which implicates its role in prostate carcinogenesis in this experimental model. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-12-22 2015-08-28 2016-06-22T18:31:54Z 2016-06-22T18:31:54Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
GALHEIGO, Maria Raquel Unterkircher. Carcinogênese da próstata de camundongos: papel do receptor tipo I do TNF-α na incidência tumoral. 2015. 57 f. Dissertação (Mestrado em Ciências Biomédicas) - Universidade Federal de Uberlândia, Uberlândia, 2015. DOI https://doi.org/10.14393/ufu.di.2015.444 https://repositorio.ufu.br/handle/123456789/12415 https://doi.org/10.14393/ufu.di.2015.444 |
identifier_str_mv |
GALHEIGO, Maria Raquel Unterkircher. Carcinogênese da próstata de camundongos: papel do receptor tipo I do TNF-α na incidência tumoral. 2015. 57 f. Dissertação (Mestrado em Ciências Biomédicas) - Universidade Federal de Uberlândia, Uberlândia, 2015. DOI https://doi.org/10.14393/ufu.di.2015.444 |
url |
https://repositorio.ufu.br/handle/123456789/12415 https://doi.org/10.14393/ufu.di.2015.444 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Uberlândia BR Programa de Pós-graduação em Biologia Celular e Estrutural Aplicadas Ciências Biomédicas UFU |
publisher.none.fl_str_mv |
Universidade Federal de Uberlândia BR Programa de Pós-graduação em Biologia Celular e Estrutural Aplicadas Ciências Biomédicas UFU |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFU instname:Universidade Federal de Uberlândia (UFU) instacron:UFU |
instname_str |
Universidade Federal de Uberlândia (UFU) |
instacron_str |
UFU |
institution |
UFU |
reponame_str |
Repositório Institucional da UFU |
collection |
Repositório Institucional da UFU |
repository.name.fl_str_mv |
Repositório Institucional da UFU - Universidade Federal de Uberlândia (UFU) |
repository.mail.fl_str_mv |
diinf@dirbi.ufu.br |
_version_ |
1805569623318331392 |