Remodelamento de biomarcadores imunológicos séricos no processo de envelhecimento
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFU |
Texto Completo: | https://repositorio.ufu.br/handle/123456789/34200 https://doi.org/10.14393/ufu.di.2022.64 |
Resumo: | The ageing process is a complex phenomenon that impacts the immune system, leading to changes in the pattern of serum soluble mediators. In the present study, the serum levels of several chemokines, pro-inflammatory/regulatory cytokines and growth factors were quantified by high-throughput microbeads array in serum samples from 541 healthy subjects at distinct age ranges (3Yrs to >70Yrs). A broad increase in serum soluble mediators were observed at 6-10Yrs with subsequent decline at 11-20Yrs and 21-30Yrs followed by a second round of upregulation starting at 31-40Yrs, with broad increase at 51-60Yrs and a marked decline at age >70Yrs. Heatmap and signatures of serum soluble mediators demonstrated a bimodal profile with one peak at 6-10Yrs and a second wave around 61-70Yrs. A universal decline was observed later at age >70Yrs. In males, the second wave started earlier at 31-40Yrs with a peak at 51-60Yrs and a further smooth decline towards >70Yrs. Conversely, in females the first peak extended from 3-5Yrs to 6-10Yrs and the second wave starting around 41-50Yrs with a peak at 61-70Yrs followed by a sharp decline at >70Yrs. Overall, CCL11, CXCL8, IL-1β, IL-6 were underscored as universal age-related biomarkers with higher levels observed at later age ranges (after 31-40Yrs) and TNF with increased levels starting at early age ranges. Data analysis demonstrated that the highest neighborhood connectivity amongst soluble mediators occurred at 3-5Yrs, with distinct declining and strengthening rhythm in males and females. Notably, rebuilding re-arrangements were usually earlier and more frequent in females (at 11-20Yrs, 51-60Yrs and >70Yrs) than in males (at 21-30Yrs, 61-70Yrs). Overall, this study provided a comprehensive landscape of evidence that distinct waves, rhythms and dynamic network connectivity are observed along healthy ageing with differences in magnitude and timing reported for sexes. |
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Remodelamento de biomarcadores imunológicos séricos no processo de envelhecimentoRemodeling of serum immune biomarkers in the aging processQuimiocinasCitocinasFatores de CrescimentoEnvelhecimentoSexoChemokines;CytokinesGrowth FactorsAgeingSexesCNPQ::CIENCIAS BIOLOGICAS::IMUNOLOGIA::IMUNOLOGIA APLICADACNPQ::CIENCIAS BIOLOGICAS::IMUNOLOGIA::IMUNOLOGIA CELULARImunologiaEnvelhecimentoHomens idososMulheres - EnvelhecimentoThe ageing process is a complex phenomenon that impacts the immune system, leading to changes in the pattern of serum soluble mediators. In the present study, the serum levels of several chemokines, pro-inflammatory/regulatory cytokines and growth factors were quantified by high-throughput microbeads array in serum samples from 541 healthy subjects at distinct age ranges (3Yrs to >70Yrs). A broad increase in serum soluble mediators were observed at 6-10Yrs with subsequent decline at 11-20Yrs and 21-30Yrs followed by a second round of upregulation starting at 31-40Yrs, with broad increase at 51-60Yrs and a marked decline at age >70Yrs. Heatmap and signatures of serum soluble mediators demonstrated a bimodal profile with one peak at 6-10Yrs and a second wave around 61-70Yrs. A universal decline was observed later at age >70Yrs. In males, the second wave started earlier at 31-40Yrs with a peak at 51-60Yrs and a further smooth decline towards >70Yrs. Conversely, in females the first peak extended from 3-5Yrs to 6-10Yrs and the second wave starting around 41-50Yrs with a peak at 61-70Yrs followed by a sharp decline at >70Yrs. Overall, CCL11, CXCL8, IL-1β, IL-6 were underscored as universal age-related biomarkers with higher levels observed at later age ranges (after 31-40Yrs) and TNF with increased levels starting at early age ranges. Data analysis demonstrated that the highest neighborhood connectivity amongst soluble mediators occurred at 3-5Yrs, with distinct declining and strengthening rhythm in males and females. Notably, rebuilding re-arrangements were usually earlier and more frequent in females (at 11-20Yrs, 51-60Yrs and >70Yrs) than in males (at 21-30Yrs, 61-70Yrs). Overall, this study provided a comprehensive landscape of evidence that distinct waves, rhythms and dynamic network connectivity are observed along healthy ageing with differences in magnitude and timing reported for sexes.CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorDissertação (Mestrado)O processo de envelhecimento é um fenômeno complexo que impactam no sistema imune, levando a mudanças nos padrões de secreção de marcadores séricos solúveis. No presente trabalho, os níveis séricos de diversas quimiocinas, fatores de crescimento, citocinas pró-inflamatórias e reguladoras foram quantificadas por meio de microesferas de alto rendimento em amostras de soro provenientes de 541 indivíduos saudáveis em diferentes faixas etárias (3Anos a >70Anos). Um grande aumento nos marcadores séricos foi observado em 6-10Anos seguido de um declínio em 11-20Anos e 21-30Anos, seguido de uma segunda fase de regulação positiva, que se inicia em 31-40Anos, com subsequente aumento em 51-60Anos e um grande declínio em >70Anos. Em homens, uma segunda onda se inicia precocemente, em 31-40Anos, com pico em 51-60Anos e um declínio suave em >70Anos. De forma diferente, nas mulheres o primeiro pico se estende de 3-5Anos até 6-10Anos e uma segunda onda tem início por volta de 41-50Anos, sendo o pico em 61-70Anos, e em >70Anos é observado uma forte queda. Assim, de forma geral, CCL11, CXCL8, IL-1β e IL-6 se destacam como biomarcadores ligados a idade universais, logo, durante o envelhecimento saudável, esses marcadores apresentam níveis aumentados após 31-40Anos e TNF apresenta níveis aumentados nas primeiras faixas etárias. A análise de dados demonstrou que a maior conectividade de biomarcadores com a vizinhança ocorre em 3-5Anos, com ritmos distintos de declínio e recuperação entre homens e mulheres. Interessantemente, os rearranjos de reconstrução das redes são mais comuns e mais frequentes nas mulheres (11-20Anos, 51-60Anos e >70Anos) em relação aos homens (21-30Anos e 61-70Anos). De forma geral, esse estudo fornece um panorama abrangente de evidências que ondas, ritmos e redes de conexões distintas são observados durante o envelhecimento saudável, com diferenças de magnitude e tempo relatadas para os sexos.Universidade Federal de UberlândiaBrasilPrograma de Pós-graduação em Imunologia e Parasitologia AplicadasGomes, Angelica de Oliveirahttp://lattes.cnpq.br/7824516128256935Martins Filho, Olindo Assishttp://lattes.cnpq.br/6260226537155026Abreu, Gabriela Silveira Nuneshttp://lattes.cnpq.br/6661555765981790Navarro, Karen Cecília de Lima Torreshttp://lattes.cnpq.br/0404523893145501Sousa, Joaquim Pedro Brito de2022-03-07T18:46:05Z2022-03-07T18:46:05Z2022-02-22info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfSOUSA, Joaquim Pedro Brito de. Remodelamento de biomarcadores imunológicos séricos no processo de envelhecimento. 2022. 69 f. Dissertação (Mestrado em Imunologia e Parasitologia Aplicadas) - Universidade Federal de Uberlândia, Uberlândia, 2022. DOI http://doi.org/10.14393/ufu.di.2022.64.https://repositorio.ufu.br/handle/123456789/34200https://doi.org/10.14393/ufu.di.2022.64porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFUinstname:Universidade Federal de Uberlândia (UFU)instacron:UFU2023-03-01T03:00:00Zoai:repositorio.ufu.br:123456789/34200Repositório InstitucionalONGhttp://repositorio.ufu.br/oai/requestdiinf@dirbi.ufu.bropendoar:2023-03-01T03:00Repositório Institucional da UFU - Universidade Federal de Uberlândia (UFU)false |
dc.title.none.fl_str_mv |
Remodelamento de biomarcadores imunológicos séricos no processo de envelhecimento Remodeling of serum immune biomarkers in the aging process |
title |
Remodelamento de biomarcadores imunológicos séricos no processo de envelhecimento |
spellingShingle |
Remodelamento de biomarcadores imunológicos séricos no processo de envelhecimento Sousa, Joaquim Pedro Brito de Quimiocinas Citocinas Fatores de Crescimento Envelhecimento Sexo Chemokines; Cytokines Growth Factors Ageing Sexes CNPQ::CIENCIAS BIOLOGICAS::IMUNOLOGIA::IMUNOLOGIA APLICADA CNPQ::CIENCIAS BIOLOGICAS::IMUNOLOGIA::IMUNOLOGIA CELULAR Imunologia Envelhecimento Homens idosos Mulheres - Envelhecimento |
title_short |
Remodelamento de biomarcadores imunológicos séricos no processo de envelhecimento |
title_full |
Remodelamento de biomarcadores imunológicos séricos no processo de envelhecimento |
title_fullStr |
Remodelamento de biomarcadores imunológicos séricos no processo de envelhecimento |
title_full_unstemmed |
Remodelamento de biomarcadores imunológicos séricos no processo de envelhecimento |
title_sort |
Remodelamento de biomarcadores imunológicos séricos no processo de envelhecimento |
author |
Sousa, Joaquim Pedro Brito de |
author_facet |
Sousa, Joaquim Pedro Brito de |
author_role |
author |
dc.contributor.none.fl_str_mv |
Gomes, Angelica de Oliveira http://lattes.cnpq.br/7824516128256935 Martins Filho, Olindo Assis http://lattes.cnpq.br/6260226537155026 Abreu, Gabriela Silveira Nunes http://lattes.cnpq.br/6661555765981790 Navarro, Karen Cecília de Lima Torres http://lattes.cnpq.br/0404523893145501 |
dc.contributor.author.fl_str_mv |
Sousa, Joaquim Pedro Brito de |
dc.subject.por.fl_str_mv |
Quimiocinas Citocinas Fatores de Crescimento Envelhecimento Sexo Chemokines; Cytokines Growth Factors Ageing Sexes CNPQ::CIENCIAS BIOLOGICAS::IMUNOLOGIA::IMUNOLOGIA APLICADA CNPQ::CIENCIAS BIOLOGICAS::IMUNOLOGIA::IMUNOLOGIA CELULAR Imunologia Envelhecimento Homens idosos Mulheres - Envelhecimento |
topic |
Quimiocinas Citocinas Fatores de Crescimento Envelhecimento Sexo Chemokines; Cytokines Growth Factors Ageing Sexes CNPQ::CIENCIAS BIOLOGICAS::IMUNOLOGIA::IMUNOLOGIA APLICADA CNPQ::CIENCIAS BIOLOGICAS::IMUNOLOGIA::IMUNOLOGIA CELULAR Imunologia Envelhecimento Homens idosos Mulheres - Envelhecimento |
description |
The ageing process is a complex phenomenon that impacts the immune system, leading to changes in the pattern of serum soluble mediators. In the present study, the serum levels of several chemokines, pro-inflammatory/regulatory cytokines and growth factors were quantified by high-throughput microbeads array in serum samples from 541 healthy subjects at distinct age ranges (3Yrs to >70Yrs). A broad increase in serum soluble mediators were observed at 6-10Yrs with subsequent decline at 11-20Yrs and 21-30Yrs followed by a second round of upregulation starting at 31-40Yrs, with broad increase at 51-60Yrs and a marked decline at age >70Yrs. Heatmap and signatures of serum soluble mediators demonstrated a bimodal profile with one peak at 6-10Yrs and a second wave around 61-70Yrs. A universal decline was observed later at age >70Yrs. In males, the second wave started earlier at 31-40Yrs with a peak at 51-60Yrs and a further smooth decline towards >70Yrs. Conversely, in females the first peak extended from 3-5Yrs to 6-10Yrs and the second wave starting around 41-50Yrs with a peak at 61-70Yrs followed by a sharp decline at >70Yrs. Overall, CCL11, CXCL8, IL-1β, IL-6 were underscored as universal age-related biomarkers with higher levels observed at later age ranges (after 31-40Yrs) and TNF with increased levels starting at early age ranges. Data analysis demonstrated that the highest neighborhood connectivity amongst soluble mediators occurred at 3-5Yrs, with distinct declining and strengthening rhythm in males and females. Notably, rebuilding re-arrangements were usually earlier and more frequent in females (at 11-20Yrs, 51-60Yrs and >70Yrs) than in males (at 21-30Yrs, 61-70Yrs). Overall, this study provided a comprehensive landscape of evidence that distinct waves, rhythms and dynamic network connectivity are observed along healthy ageing with differences in magnitude and timing reported for sexes. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-03-07T18:46:05Z 2022-03-07T18:46:05Z 2022-02-22 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
SOUSA, Joaquim Pedro Brito de. Remodelamento de biomarcadores imunológicos séricos no processo de envelhecimento. 2022. 69 f. Dissertação (Mestrado em Imunologia e Parasitologia Aplicadas) - Universidade Federal de Uberlândia, Uberlândia, 2022. DOI http://doi.org/10.14393/ufu.di.2022.64. https://repositorio.ufu.br/handle/123456789/34200 https://doi.org/10.14393/ufu.di.2022.64 |
identifier_str_mv |
SOUSA, Joaquim Pedro Brito de. Remodelamento de biomarcadores imunológicos séricos no processo de envelhecimento. 2022. 69 f. Dissertação (Mestrado em Imunologia e Parasitologia Aplicadas) - Universidade Federal de Uberlândia, Uberlândia, 2022. DOI http://doi.org/10.14393/ufu.di.2022.64. |
url |
https://repositorio.ufu.br/handle/123456789/34200 https://doi.org/10.14393/ufu.di.2022.64 |
dc.language.iso.fl_str_mv |
por |
language |
por |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Uberlândia Brasil Programa de Pós-graduação em Imunologia e Parasitologia Aplicadas |
publisher.none.fl_str_mv |
Universidade Federal de Uberlândia Brasil Programa de Pós-graduação em Imunologia e Parasitologia Aplicadas |
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reponame:Repositório Institucional da UFU instname:Universidade Federal de Uberlândia (UFU) instacron:UFU |
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Universidade Federal de Uberlândia (UFU) |
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UFU |
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Repositório Institucional da UFU |
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Repositório Institucional da UFU - Universidade Federal de Uberlândia (UFU) |
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diinf@dirbi.ufu.br |
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