Avaliação das citocinas IL-6 e TNF-alfa no soro de pacientes com câncer de mama

• Autor(a) principal: Silva, Lucas Eduardo Correia da
• Data de Publicação: 2019
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UFU
• Texto Completo: https://repositorio.ufu.br/handle/123456789/28520
• topic: Câncer de mama; Citocinas; IL-6; TNF-alfa; LegendPlex; Receptores hormonais; Subtipo molecular; Estadiamento tumoral; Grau tumoral; Breast cancer; Cytokines; Hormone receptors; Molecular subtype; Tumor staging; Tumor grade; CNPQ::CIENCIAS DA SAUDE

Introduction: Breast cancer is one of the leading causes of death worldwide, being more frequent in women. It can be classified by hormone receptors, staging, grade, molecular subtype, metastasis and among other variables. Generally speaking, cytokines involve a large group of molecules that are capable of evoking effects on cells in an immune response and many of them are involved in tumor development, whether anti or pro-tumor. TNF-α and IL-6 have their dichotomous role shown in several studies, since both are proinflammatory cytokines and their primary function is to maintain the body's homeostasis, but they may assume a pro-tumoral role depending factors such as: tumor type, tumor microenvironment, interference of other cytokines, deregulation in its production, among others. Therefore, establishing a relationship between these cytokines and some clinical factors of patients may provide important information to analyze the tumor biomarker potential of these cytokines. Objective: This study aimed to quantify IL-6 and TNF-afa in the serum of breast cancer patients and to analyze their relationship with some clinical variables. Methodology: Flow cytometry was used with LegendPlex, a kit based on sandwich immunoassay principles to detect and quantify these cytokines in the serum of 80 breast cancer patients. Results: The results obtained show that there is no relationship between TNF-alpha and IL-6 cytokines with clinical variables. Conclusion: It was concluded that there is no significant relationship between the analyzed cytokines and the clinical variables.