Effects of diabetes mellitus and antioxidant supplementation in oxidative stress and expression of myosin in rat brain

Detalhes bibliográficos
Autor(a) principal: Calábria, Luciana Karen
Data de Publicação: 2010
Tipo de documento: Tese
Idioma: eng
Título da fonte: Repositório Institucional da UFU
Texto Completo: https://repositorio.ufu.br/handle/123456789/15714
Resumo: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
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spelling Effects of diabetes mellitus and antioxidant supplementation in oxidative stress and expression of myosin in rat brainEfeitos do diabetes mellitus e da suplementação com antioxidantes no estresse ocidativo e na expressão de miosinas no cérebro de ratosAntioxidantesMiosinaDiabetes mellitusBrainCalciumCalmodulinMyosin-IIBCNPQ::CIENCIAS BIOLOGICAS::GENETICACoordenação de Aperfeiçoamento de Pessoal de Nível SuperiorDoutor em Genética e BioquímicaCHAPTER II: Ca+2/calmodulin complex interacts with and regulates various enzymes and target proteins, known as calmodulin-binding proteins (CaMBPs). This group of proteins includes molecular motors such as myosins. Non-muscle myosin-IIB was shown in our study to be overexpressed in diabetic rat brains. In this study, we compared CaMBPs, purified by immobilized-calmodulin, affinity chromatography, isolated from non-diabetic and streptozotocin-induced diabetic rat brains. Proteins eluted with EGTA and urea were separated on a SDS-PAGE gel, digested and submitted to peptide mass fingerprinting analysis. There were fifteen and sixteen intense bands for non-diabetic and diabetic brains, respectively. Two proteins were exclusively in non-diabetic brains, four were found exclusively in diabetic brains and thirteen were found in both. Protein sequences were then analyzed for the presence of the calmodulin-binding sites. A large fraction of the eluted proteins (83%) had putative IQ motifs or calmodulin-binding sites. The myosin-IIB affinity chromatography elution in association with western blot, immunohistochemistry and RT-PCR analyses strongly suggest that myosin-IIB protein and mRNA are highly expressed levels in diabetic rat brains. This is the first study that identified and showed calmodulin-binding proteins differential expression in non-diabetic and diabetic rat brains through a comparative proteomic analysis, and it opens up a new field of study relating myosin-IIB expression in the brain, diabetes mellitus and intracellular calcium regulation. CHAPTER III: Diabetes mellitus is a chronic disease characterized by persistent hyperglycemia, which may lead to brain tissue damage due to oxidative stress. The enhancement of free radicals during hyperglycemia contributes to increased neuronal death and changes in synaptic transmission. This study evaluated the effect of oxidative stress and the use of antioxidants (calcium, zinc and vitamin E) supplementation on myosins expression levels in brain tissues of streptozotocin-induced diabetes experimental rat model. Lipid peroxidation, antioxidant enzymes activities and myosins IIB and Va expressions at transcriptional and protein levels were examined after 5 and 90 days post streptozotocin induction. The chronic effect of the streptozotocininduced diabetes led to upregulation of glutathione peroxidase (GPx) and malondialdehyde (MDA) levels, and downregulation of superoxide dismutase (SOD) and catalase (CAT) activities. These alterations were accompanied by increased myosin-IIB and decreased myosin-Va expressions in diabetic rat brains. The antioxidant supplementation protected against hyperglycemia damage by reducing CAT activity and increasing GPx and SOD activities, although it had no influence on MDA levels. Interestingly, only diabetic rats restored myosin-IIB expression at both transcriptional and protein levels in brain tissues after supplementation, and no differences were observed in myosin-Va expression in comparison to non-diabetic rat brains. The functional alterations of antioxidant enzymes and myosins IIB and Va in rat brains of experimentallyinduced diabetes were strongly associated with the disease and aging during acute and chronic stages, but only myosin-IIB expression could be reversed by antioxidants. These data highlight the supplementation (calcium, zinc and vitamin E) antioxidants effects on the oxidative stress and the involvement of myosins IIB and Va in the diabetes stages; however, their mechanisms of action on enzymes activities, lipid peroxidation and myosins expression remain to be elucidated.Universidade Federal de UberlândiaBRPrograma de Pós-graduação em Genética e BioquímicaCiências BiológicasUFUEspindola, Foued Salmenhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4727087Y6Taketomi, Ernesto Akiohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4788625D6Jorge, Paulo Tannushttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4737314J9Antunes, Lusânia Maria Greggihttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4728366P5Botelho, Françoise Vasconceloshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4794713P7Lamers, Marcelo Lazzaronhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4705907Z6Calábria, Luciana Karen2016-06-22T18:43:23Z2010-10-202016-06-22T18:43:23Z2010-08-06info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfapplication/pdfCALÁBRIA, Luciana Karen. Efeitos do diabetes mellitus e da suplementação com antioxidantes no estresse ocidativo e na expressão de miosinas no cérebro de ratos. 2010. 108 f. Tese (Doutorado em Ciências Biológicas) - Universidade Federal de Uberlândia, Uberlândia, 2010.https://repositorio.ufu.br/handle/123456789/15714enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFUinstname:Universidade Federal de Uberlândia (UFU)instacron:UFU2016-06-23T07:13:34Zoai:repositorio.ufu.br:123456789/15714Repositório InstitucionalONGhttp://repositorio.ufu.br/oai/requestdiinf@dirbi.ufu.bropendoar:2016-06-23T07:13:34Repositório Institucional da UFU - Universidade Federal de Uberlândia (UFU)false
dc.title.none.fl_str_mv Effects of diabetes mellitus and antioxidant supplementation in oxidative stress and expression of myosin in rat brain
Efeitos do diabetes mellitus e da suplementação com antioxidantes no estresse ocidativo e na expressão de miosinas no cérebro de ratos
title Effects of diabetes mellitus and antioxidant supplementation in oxidative stress and expression of myosin in rat brain
spellingShingle Effects of diabetes mellitus and antioxidant supplementation in oxidative stress and expression of myosin in rat brain
Calábria, Luciana Karen
Antioxidantes
Miosina
Diabetes mellitus
Brain
Calcium
Calmodulin
Myosin-IIB
CNPQ::CIENCIAS BIOLOGICAS::GENETICA
title_short Effects of diabetes mellitus and antioxidant supplementation in oxidative stress and expression of myosin in rat brain
title_full Effects of diabetes mellitus and antioxidant supplementation in oxidative stress and expression of myosin in rat brain
title_fullStr Effects of diabetes mellitus and antioxidant supplementation in oxidative stress and expression of myosin in rat brain
title_full_unstemmed Effects of diabetes mellitus and antioxidant supplementation in oxidative stress and expression of myosin in rat brain
title_sort Effects of diabetes mellitus and antioxidant supplementation in oxidative stress and expression of myosin in rat brain
author Calábria, Luciana Karen
author_facet Calábria, Luciana Karen
author_role author
dc.contributor.none.fl_str_mv Espindola, Foued Salmen
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4727087Y6
Taketomi, Ernesto Akio
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4788625D6
Jorge, Paulo Tannus
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4737314J9
Antunes, Lusânia Maria Greggi
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4728366P5
Botelho, Françoise Vasconcelos
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4794713P7
Lamers, Marcelo Lazzaron
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4705907Z6
dc.contributor.author.fl_str_mv Calábria, Luciana Karen
dc.subject.por.fl_str_mv Antioxidantes
Miosina
Diabetes mellitus
Brain
Calcium
Calmodulin
Myosin-IIB
CNPQ::CIENCIAS BIOLOGICAS::GENETICA
topic Antioxidantes
Miosina
Diabetes mellitus
Brain
Calcium
Calmodulin
Myosin-IIB
CNPQ::CIENCIAS BIOLOGICAS::GENETICA
description Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
publishDate 2010
dc.date.none.fl_str_mv 2010-10-20
2010-08-06
2016-06-22T18:43:23Z
2016-06-22T18:43:23Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv CALÁBRIA, Luciana Karen. Efeitos do diabetes mellitus e da suplementação com antioxidantes no estresse ocidativo e na expressão de miosinas no cérebro de ratos. 2010. 108 f. Tese (Doutorado em Ciências Biológicas) - Universidade Federal de Uberlândia, Uberlândia, 2010.
https://repositorio.ufu.br/handle/123456789/15714
identifier_str_mv CALÁBRIA, Luciana Karen. Efeitos do diabetes mellitus e da suplementação com antioxidantes no estresse ocidativo e na expressão de miosinas no cérebro de ratos. 2010. 108 f. Tese (Doutorado em Ciências Biológicas) - Universidade Federal de Uberlândia, Uberlândia, 2010.
url https://repositorio.ufu.br/handle/123456789/15714
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Uberlândia
BR
Programa de Pós-graduação em Genética e Bioquímica
Ciências Biológicas
UFU
publisher.none.fl_str_mv Universidade Federal de Uberlândia
BR
Programa de Pós-graduação em Genética e Bioquímica
Ciências Biológicas
UFU
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFU
instname:Universidade Federal de Uberlândia (UFU)
instacron:UFU
instname_str Universidade Federal de Uberlândia (UFU)
instacron_str UFU
institution UFU
reponame_str Repositório Institucional da UFU
collection Repositório Institucional da UFU
repository.name.fl_str_mv Repositório Institucional da UFU - Universidade Federal de Uberlândia (UFU)
repository.mail.fl_str_mv diinf@dirbi.ufu.br
_version_ 1813711507175768064

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