Efeito do tratamento com Vochysia rufa Mart. e glibenclamida sobre o estresse oxidativo e a expressão de proteínas motoras e de ancoragem em cérebro de ratos diabéticos
Autor(a) principal: | |
---|---|
Data de Publicação: | 2013 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFU |
Texto Completo: | https://repositorio.ufu.br/handle/123456789/15872 |
Resumo: | CHAPTER II: Myosin-IIB is a non-muscle isoform in the brain with increased expression in the brains of diabetic rats. Chronic hyperglycemia caused by diabetes can impair learning and memory. Oral hypoglycemic agents such as glibenclamide have been used to control hyperglycemia. We report changes in the expression and distribution of myosin-IIB in the frontal cortex and hippocampus of diabetic rats treated with glibenclamide. To establish this, brains were harvested after 43 days of treatment with glibenclamide (6 mg/kg bw orally), homogenized and analyzed by Western blotting, qRT-PCR and immunohistochemistry. As expected, myosin-IIB expression increased in the brains of diabetic rats. However, protein levels returned to normalcy after treatment with glibenclamide. In addition, MYH10 gene expression decreased in diabetic rats treated with glibenclamide. Moreover, we found weak myosin-IIB labeling in the hippocampus and frontal cortex of rats treated with glibenclamide. Therefore, the expression of myosin-IIB is affected by diabetes mellitus and may be modulated by glibenclamide treatment in rats. Structural changes in the hippocampus and prefrontal cortex are reversible, and glibenclamide treatment may reduce patho-physiological changes in the brain. Our findings suggest a possible correlation between glibenclamide effects and myosin-IIB function in the brain of diabetised rats. CHAPTER III: Introduction: Diabetes increases oxidative stress and causes several changes in protein transport and docking of synaptic vesicles. Plant extracts have long been used to treat hyperglycemia and diabetes. Aqueous extracts of Vochysia rufa, a species endemic to the Brazilian Cerrado ecossistem, have increasingly been prescribed and used as a complementary or alternative treatment. Objective: We examined the effects of an aqueous extract of Vochysia rufa on oxidative stress markers and on the expression and localization of transport proteins and synaptic vesicle docking in the diabetic rat brain. Design/Methods: Thirty-two male Wister rats were randomly divided into 4 groups: non-diabetic, diabetic, diabetic treated with glibenclamide (6 mg / kg bw orally) and diabetic treated with Vochysia rufa extract (500 mg/kg bw orally) for forty-three days. After the treatments, brains were collected and the following parameters were evaluated: enzymatic activity of glutathione peroxidase and glutathione S-transferase, superoxide dismutase concentration, total sulfhydryl, lipid peroxidation and reduced glutathione. The levels and localization of proteins such as CaMKII, myosin-Va, synapsin-1, SNAP-25 and GLUT4 were also analyzed. Results: Vochysia rufa extract decreased SOD, GSH, TBARS and total sulfhydryl levels and increased GST levels. Additionally, myosin-Va and synapsin-1 expression decreased and levels proteins such as CaMKII and SNAP-25 increased. These results were confirmed by immunolocalization. Conclusion: The results suggest that an aqueous extract of Vochysia rufa has a neuroprotective effect on diabetic rat brains by reducing oxidative stress and controlling changes in myosin-Va and synapsin-1 expression. |
id |
UFU_7500ec334a719a748f27ea4c41ecc951 |
---|---|
oai_identifier_str |
oai:repositorio.ufu.br:123456789/15872 |
network_acronym_str |
UFU |
network_name_str |
Repositório Institucional da UFU |
repository_id_str |
|
spelling |
Efeito do tratamento com Vochysia rufa Mart. e glibenclamida sobre o estresse oxidativo e a expressão de proteínas motoras e de ancoragem em cérebro de ratos diabéticosDiabetesMiosinasDiabetes mellitusStreptozotocinGlibenclamideBrainMyosin-IIBHippocampusOxidative stressMyosin-vaCaMKIISNAP-25Synapsin-1GLUT4.CNPQ::CIENCIAS BIOLOGICAS::GENETICACHAPTER II: Myosin-IIB is a non-muscle isoform in the brain with increased expression in the brains of diabetic rats. Chronic hyperglycemia caused by diabetes can impair learning and memory. Oral hypoglycemic agents such as glibenclamide have been used to control hyperglycemia. We report changes in the expression and distribution of myosin-IIB in the frontal cortex and hippocampus of diabetic rats treated with glibenclamide. To establish this, brains were harvested after 43 days of treatment with glibenclamide (6 mg/kg bw orally), homogenized and analyzed by Western blotting, qRT-PCR and immunohistochemistry. As expected, myosin-IIB expression increased in the brains of diabetic rats. However, protein levels returned to normalcy after treatment with glibenclamide. In addition, MYH10 gene expression decreased in diabetic rats treated with glibenclamide. Moreover, we found weak myosin-IIB labeling in the hippocampus and frontal cortex of rats treated with glibenclamide. Therefore, the expression of myosin-IIB is affected by diabetes mellitus and may be modulated by glibenclamide treatment in rats. Structural changes in the hippocampus and prefrontal cortex are reversible, and glibenclamide treatment may reduce patho-physiological changes in the brain. Our findings suggest a possible correlation between glibenclamide effects and myosin-IIB function in the brain of diabetised rats. CHAPTER III: Introduction: Diabetes increases oxidative stress and causes several changes in protein transport and docking of synaptic vesicles. Plant extracts have long been used to treat hyperglycemia and diabetes. Aqueous extracts of Vochysia rufa, a species endemic to the Brazilian Cerrado ecossistem, have increasingly been prescribed and used as a complementary or alternative treatment. Objective: We examined the effects of an aqueous extract of Vochysia rufa on oxidative stress markers and on the expression and localization of transport proteins and synaptic vesicle docking in the diabetic rat brain. Design/Methods: Thirty-two male Wister rats were randomly divided into 4 groups: non-diabetic, diabetic, diabetic treated with glibenclamide (6 mg / kg bw orally) and diabetic treated with Vochysia rufa extract (500 mg/kg bw orally) for forty-three days. After the treatments, brains were collected and the following parameters were evaluated: enzymatic activity of glutathione peroxidase and glutathione S-transferase, superoxide dismutase concentration, total sulfhydryl, lipid peroxidation and reduced glutathione. The levels and localization of proteins such as CaMKII, myosin-Va, synapsin-1, SNAP-25 and GLUT4 were also analyzed. Results: Vochysia rufa extract decreased SOD, GSH, TBARS and total sulfhydryl levels and increased GST levels. Additionally, myosin-Va and synapsin-1 expression decreased and levels proteins such as CaMKII and SNAP-25 increased. These results were confirmed by immunolocalization. Conclusion: The results suggest that an aqueous extract of Vochysia rufa has a neuroprotective effect on diabetic rat brains by reducing oxidative stress and controlling changes in myosin-Va and synapsin-1 expression.Fundação de Amparo a Pesquisa do Estado de Minas GeraisMestre em Genética e BioquímicaO diabetes aumenta o estresse oxidativo e provoca várias alterações no transporte de proteínas e de ancoragem das vesículas sinápticas. Os extratos de plantas têm sido muito utilizados para tratar a hiperglicemia e diabetes. Extratos aquosos de Vochysia rufa, uma espécie do Cerrado brasileiro, têm sido prescritos e utilizados como tratamento complementar ou alternativo. Foram analisados os efeitos do extrato aquoso de Vochysia rufa sobre marcadores de estresse oxidativo e na expressão e localização de proteínas de transporte e ancoragem das vesículas sinápticas no cérebro de ratos diabéticos. Trinta e dois ratos Wistar machos foram divididos aleatoriamente em 4 grupos: não-diabéticos, diabéticos, diabéticos tratados com glibenclamida (6 mg/kg de peso corporal por via oral) e diabéticos tratados com extrato de Vochysia rufa (500 mg/kg de peso corporal por via oral) por 43 dias. Após os tratamentos, os cérebros foram coletadas e os seguintes parâmetros foram avaliados: atividade enzimática da glutationa peroxidase e glutationa S- transferase, a concentração de superóxido dismutase, sulfidrila total, a peroxidação lipídica e de glutationa reduzida. Os níveis e localização de proteínas, como CaMKII, miosina - Va, sinapsina -1, SNAP -25 e GLUT4 também foram analisados. O extrato de Vochysia rufa diminuiu SOD, GSH, TBARS e os níveis totais de sulfidrila e aumentou os níveis de GST. Além disso, a miosina -Va e sinapsina -1 apresentaram os níveis de expressão diminuídas, ao contrário de CaMKII e SNAP-25 que aumentaram. Estes resultados foram confirmados por imunohistoquímica. Os resultados sugerem que o extrato aquoso de Vochysia rufa tem um efeito neuroprotetor em cérebros de ratos diabéticos, reduzindo o estresse oxidativo e controlando as mudanças na miosina -Va e sinapsina -1.Universidade Federal de UberlândiaBRPrograma de Pós-graduação em Genética e BioquímicaCiências BiológicasUFUCalábria, Luciana Karenhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4776153E6Espindola, Foued Salmenhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4727087Y6Peixoto, Pablo Marco Verashttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4703659Y7Gonçalves, Reggiani Vilelahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4139303E6Costa, Alice Vieira da2016-06-22T18:43:51Z2014-06-122016-06-22T18:43:51Z2013-07-29info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfCOSTA, Alice Vieira da. Efeito do tratamento com Vochysia rufa Mart. e glibenclamida sobre o estresse oxidativo e a expressão de proteínas motoras e de ancoragem em cérebro de ratos diabéticos. 2013. 80 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Uberlândia, Uberlândia, 2013.https://repositorio.ufu.br/handle/123456789/15872porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFUinstname:Universidade Federal de Uberlândia (UFU)instacron:UFU2016-06-23T07:23:24Zoai:repositorio.ufu.br:123456789/15872Repositório InstitucionalONGhttp://repositorio.ufu.br/oai/requestdiinf@dirbi.ufu.bropendoar:2016-06-23T07:23:24Repositório Institucional da UFU - Universidade Federal de Uberlândia (UFU)false |
dc.title.none.fl_str_mv |
Efeito do tratamento com Vochysia rufa Mart. e glibenclamida sobre o estresse oxidativo e a expressão de proteínas motoras e de ancoragem em cérebro de ratos diabéticos |
title |
Efeito do tratamento com Vochysia rufa Mart. e glibenclamida sobre o estresse oxidativo e a expressão de proteínas motoras e de ancoragem em cérebro de ratos diabéticos |
spellingShingle |
Efeito do tratamento com Vochysia rufa Mart. e glibenclamida sobre o estresse oxidativo e a expressão de proteínas motoras e de ancoragem em cérebro de ratos diabéticos Costa, Alice Vieira da Diabetes Miosinas Diabetes mellitus Streptozotocin Glibenclamide Brain Myosin-IIB Hippocampus Oxidative stress Myosin-va CaMKII SNAP-25 Synapsin-1 GLUT4. CNPQ::CIENCIAS BIOLOGICAS::GENETICA |
title_short |
Efeito do tratamento com Vochysia rufa Mart. e glibenclamida sobre o estresse oxidativo e a expressão de proteínas motoras e de ancoragem em cérebro de ratos diabéticos |
title_full |
Efeito do tratamento com Vochysia rufa Mart. e glibenclamida sobre o estresse oxidativo e a expressão de proteínas motoras e de ancoragem em cérebro de ratos diabéticos |
title_fullStr |
Efeito do tratamento com Vochysia rufa Mart. e glibenclamida sobre o estresse oxidativo e a expressão de proteínas motoras e de ancoragem em cérebro de ratos diabéticos |
title_full_unstemmed |
Efeito do tratamento com Vochysia rufa Mart. e glibenclamida sobre o estresse oxidativo e a expressão de proteínas motoras e de ancoragem em cérebro de ratos diabéticos |
title_sort |
Efeito do tratamento com Vochysia rufa Mart. e glibenclamida sobre o estresse oxidativo e a expressão de proteínas motoras e de ancoragem em cérebro de ratos diabéticos |
author |
Costa, Alice Vieira da |
author_facet |
Costa, Alice Vieira da |
author_role |
author |
dc.contributor.none.fl_str_mv |
Calábria, Luciana Karen http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4776153E6 Espindola, Foued Salmen http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4727087Y6 Peixoto, Pablo Marco Veras http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4703659Y7 Gonçalves, Reggiani Vilela http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4139303E6 |
dc.contributor.author.fl_str_mv |
Costa, Alice Vieira da |
dc.subject.por.fl_str_mv |
Diabetes Miosinas Diabetes mellitus Streptozotocin Glibenclamide Brain Myosin-IIB Hippocampus Oxidative stress Myosin-va CaMKII SNAP-25 Synapsin-1 GLUT4. CNPQ::CIENCIAS BIOLOGICAS::GENETICA |
topic |
Diabetes Miosinas Diabetes mellitus Streptozotocin Glibenclamide Brain Myosin-IIB Hippocampus Oxidative stress Myosin-va CaMKII SNAP-25 Synapsin-1 GLUT4. CNPQ::CIENCIAS BIOLOGICAS::GENETICA |
description |
CHAPTER II: Myosin-IIB is a non-muscle isoform in the brain with increased expression in the brains of diabetic rats. Chronic hyperglycemia caused by diabetes can impair learning and memory. Oral hypoglycemic agents such as glibenclamide have been used to control hyperglycemia. We report changes in the expression and distribution of myosin-IIB in the frontal cortex and hippocampus of diabetic rats treated with glibenclamide. To establish this, brains were harvested after 43 days of treatment with glibenclamide (6 mg/kg bw orally), homogenized and analyzed by Western blotting, qRT-PCR and immunohistochemistry. As expected, myosin-IIB expression increased in the brains of diabetic rats. However, protein levels returned to normalcy after treatment with glibenclamide. In addition, MYH10 gene expression decreased in diabetic rats treated with glibenclamide. Moreover, we found weak myosin-IIB labeling in the hippocampus and frontal cortex of rats treated with glibenclamide. Therefore, the expression of myosin-IIB is affected by diabetes mellitus and may be modulated by glibenclamide treatment in rats. Structural changes in the hippocampus and prefrontal cortex are reversible, and glibenclamide treatment may reduce patho-physiological changes in the brain. Our findings suggest a possible correlation between glibenclamide effects and myosin-IIB function in the brain of diabetised rats. CHAPTER III: Introduction: Diabetes increases oxidative stress and causes several changes in protein transport and docking of synaptic vesicles. Plant extracts have long been used to treat hyperglycemia and diabetes. Aqueous extracts of Vochysia rufa, a species endemic to the Brazilian Cerrado ecossistem, have increasingly been prescribed and used as a complementary or alternative treatment. Objective: We examined the effects of an aqueous extract of Vochysia rufa on oxidative stress markers and on the expression and localization of transport proteins and synaptic vesicle docking in the diabetic rat brain. Design/Methods: Thirty-two male Wister rats were randomly divided into 4 groups: non-diabetic, diabetic, diabetic treated with glibenclamide (6 mg / kg bw orally) and diabetic treated with Vochysia rufa extract (500 mg/kg bw orally) for forty-three days. After the treatments, brains were collected and the following parameters were evaluated: enzymatic activity of glutathione peroxidase and glutathione S-transferase, superoxide dismutase concentration, total sulfhydryl, lipid peroxidation and reduced glutathione. The levels and localization of proteins such as CaMKII, myosin-Va, synapsin-1, SNAP-25 and GLUT4 were also analyzed. Results: Vochysia rufa extract decreased SOD, GSH, TBARS and total sulfhydryl levels and increased GST levels. Additionally, myosin-Va and synapsin-1 expression decreased and levels proteins such as CaMKII and SNAP-25 increased. These results were confirmed by immunolocalization. Conclusion: The results suggest that an aqueous extract of Vochysia rufa has a neuroprotective effect on diabetic rat brains by reducing oxidative stress and controlling changes in myosin-Va and synapsin-1 expression. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-07-29 2014-06-12 2016-06-22T18:43:51Z 2016-06-22T18:43:51Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
COSTA, Alice Vieira da. Efeito do tratamento com Vochysia rufa Mart. e glibenclamida sobre o estresse oxidativo e a expressão de proteínas motoras e de ancoragem em cérebro de ratos diabéticos. 2013. 80 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Uberlândia, Uberlândia, 2013. https://repositorio.ufu.br/handle/123456789/15872 |
identifier_str_mv |
COSTA, Alice Vieira da. Efeito do tratamento com Vochysia rufa Mart. e glibenclamida sobre o estresse oxidativo e a expressão de proteínas motoras e de ancoragem em cérebro de ratos diabéticos. 2013. 80 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Uberlândia, Uberlândia, 2013. |
url |
https://repositorio.ufu.br/handle/123456789/15872 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Uberlândia BR Programa de Pós-graduação em Genética e Bioquímica Ciências Biológicas UFU |
publisher.none.fl_str_mv |
Universidade Federal de Uberlândia BR Programa de Pós-graduação em Genética e Bioquímica Ciências Biológicas UFU |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFU instname:Universidade Federal de Uberlândia (UFU) instacron:UFU |
instname_str |
Universidade Federal de Uberlândia (UFU) |
instacron_str |
UFU |
institution |
UFU |
reponame_str |
Repositório Institucional da UFU |
collection |
Repositório Institucional da UFU |
repository.name.fl_str_mv |
Repositório Institucional da UFU - Universidade Federal de Uberlândia (UFU) |
repository.mail.fl_str_mv |
diinf@dirbi.ufu.br |
_version_ |
1805569618793725952 |