Detecção de Sclerotinia sclerotiorum em meio Neon-S modificado em sementes de soja e feijão

Detalhes bibliográficos
Autor(a) principal: Santos, Roberto Resende dos
Data de Publicação: 2015
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFU
Texto Completo: https://repositorio.ufu.br/handle/123456789/12219
https://doi.org/10.14393/ufu.di.2015.95
Resumo: The Neon-S method has been used for the detection of Sclerotinia sclerotiorum in soybean and common beans since the agricultural year 2010. However, this method yields false positive readings due to other fungi that change medium pH. Thus, this study evaluated the contribution of increasing incubation period on the optimization of Neon-S reliability in different cultivars of soybeans and common beans. Nine treatments were evaluated in randomized blocks design, as a 3x3 factorial, with three replications. The factors were three detection methods (paper roll, Neon- S, and Neon S2) and three cultivars: naturally infected Pérola beans; naturally infected soybean cultivar BRS Valiosa RR, and artificially inoculated soybean cultivar Nidera 7255 RR). Inoculation of soybean Nidera 7255 RR was done by incubating the seeds for 72 h on PDA where the fungus was previously grown for seven days at 25 ºC. The three methods were compared, evaluating 400 seeds per replication, after incubation for seven days in Neon-S, for 15 days in Neon-S2, and for 30 days in paper roll, determining the presence of the fungus, and that of sclerotia adhered to the seeds. Twenty seeds were used per plate for media Neon-S and Neon-S2 (20 plates per replication), and 50 seeds per roll for the paper roll. Seeds were incubated at 20 ºC in BDO chamber in darkness. The data were submitted to the analysis of variance and the averages compared by the Tukey test at 5% probability. The interaction between detection method and cultivar was significant at 1% probability, indicating that the best method depends on the cultivar under study. Among the cultivars evaluated, artificially infected soybean presented the greatest indices of pathogen infection. A comparison of seed lots analyzed at the Plant Disease Clinic LAMIP proved greater sensibility of Neon-S in a total of 637 lots evaluated from 2008 to 2012. In that period, the paper roll test resulted in 21.88% positive samples, while in Neon-S 31.25% of them were positive. Another distinguishing factor among the lots analyzed was the percentage of infected and, or, contaminated seeds, which varied from one (0.25%) to five (1.25%). Therefore, detection of S. sclerotiorum by the Neon-S2 method can be optimized by incubation for 15 days, considering that, in this case, the formation of sclerotia near the infected seeds confirm the presence of the pathogen, avoiding false positive readings. Neon-S2 method increased detection sensibility of S. sclerotiorum in seed lots analyzed in comparison with the paper roll method; however, in comparison with Neon S method, despite its greater average, no significant differences were observed.
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spelling Detecção de Sclerotinia sclerotiorum em meio Neon-S modificado em sementes de soja e feijãoDetection of sclerotinia sclerotiorum in modified NEON-S medium in soybean and common beans seedsGlycine maxPhaseolus vulgarisSanidade de sementesMofo brancoRolo de papelSoja - Semente - Doenças e pragasFeijão - Semente - Doenças e pragasSclerotinia sclerotiorumSeed healthWhite moldPaper rollCNPQ::CIENCIAS AGRARIAS::AGRONOMIAThe Neon-S method has been used for the detection of Sclerotinia sclerotiorum in soybean and common beans since the agricultural year 2010. However, this method yields false positive readings due to other fungi that change medium pH. Thus, this study evaluated the contribution of increasing incubation period on the optimization of Neon-S reliability in different cultivars of soybeans and common beans. Nine treatments were evaluated in randomized blocks design, as a 3x3 factorial, with three replications. The factors were three detection methods (paper roll, Neon- S, and Neon S2) and three cultivars: naturally infected Pérola beans; naturally infected soybean cultivar BRS Valiosa RR, and artificially inoculated soybean cultivar Nidera 7255 RR). Inoculation of soybean Nidera 7255 RR was done by incubating the seeds for 72 h on PDA where the fungus was previously grown for seven days at 25 ºC. The three methods were compared, evaluating 400 seeds per replication, after incubation for seven days in Neon-S, for 15 days in Neon-S2, and for 30 days in paper roll, determining the presence of the fungus, and that of sclerotia adhered to the seeds. Twenty seeds were used per plate for media Neon-S and Neon-S2 (20 plates per replication), and 50 seeds per roll for the paper roll. Seeds were incubated at 20 ºC in BDO chamber in darkness. The data were submitted to the analysis of variance and the averages compared by the Tukey test at 5% probability. The interaction between detection method and cultivar was significant at 1% probability, indicating that the best method depends on the cultivar under study. Among the cultivars evaluated, artificially infected soybean presented the greatest indices of pathogen infection. A comparison of seed lots analyzed at the Plant Disease Clinic LAMIP proved greater sensibility of Neon-S in a total of 637 lots evaluated from 2008 to 2012. In that period, the paper roll test resulted in 21.88% positive samples, while in Neon-S 31.25% of them were positive. Another distinguishing factor among the lots analyzed was the percentage of infected and, or, contaminated seeds, which varied from one (0.25%) to five (1.25%). Therefore, detection of S. sclerotiorum by the Neon-S2 method can be optimized by incubation for 15 days, considering that, in this case, the formation of sclerotia near the infected seeds confirm the presence of the pathogen, avoiding false positive readings. Neon-S2 method increased detection sensibility of S. sclerotiorum in seed lots analyzed in comparison with the paper roll method; however, in comparison with Neon S method, despite its greater average, no significant differences were observed.Mestre em AgronomiaO método de Neon-S tem sido utilizado para a detecção de Sclerotinia sclerotiorum em sementes de soja e feijão a partir da safra de 2010. Porém, esse método possibilita a leitura de falsos positivos devido ao aparecimento de fungos que também alteram o pH do meio. O objetivo deste trabalho foi verificar se o aumento do período de incubação pode contribuir para otimizar a confiabilidade do teste Neon-S em diferentes cultivares de soja e feijão. Foram avaliados nove tratamentos em delineamento de blocos casualizados no esquema fatorial 3x3, com três repetições, sendo três métodos de detecção: rolo de papel, Neon-S e Neon S2 e três cultivares: Feijão Pérola infectados naturalmente; soja cultivar BRS Valiosa RR infectadas naturalmente e soja cultivar Nidera 7255 RR inoculada artificialmente. Para a inoculação da soja Nidera 7255 RR, as sementes foram incubadas em meio de cultivo BDA por 72 h, após o cultivo do fungo por sete dias na temperatura de 25 ºC. Os três métodos foram comparados, avaliando 400 sementes por repetição, meio Neon-S com incubação de sete dias, Neon-S2 com incubação de quinze dias e o rolo de papel por trinta dias, anotando-se a presença do fungo e a presença de escleródios aderidos às sementes. No meio Neon-S e Neon-S2 foram utilizadas 20 sementes por placa (20 placas por repetição). No rolo de papel foram usadas 50 sementes por rolo. As sementes foram incubadas 20 ºC em BOD, no escuro. Realizou-se a análise de variância dos dados e teste de comparação de médias (Tukey 5%). A interação entre o método de detecção e a cultivar utilizada foi significativo a 1% de probabilidade, indicando que o melhor método depende da cultivar avaliada. Dentro das cultivares avaliadas, a soja infectada artificialmente foi a que apresentou os maiores índices de infecção pelo patógeno. Usando os resultados de lotes de sementes, comprovou-se a maior sensibilidade do Neon-S num montante de 637 lotes testados no período de 2008 a 2012. Durante esse período, o teste de rolo apresentou 21,88% de amostras positivas, enquanto que o Neon-S foi de 31,25%. Outro fator diferenciador em relação aos lotes analisados foi a porcentagem de sementes contaminadas e ou infectadas, que variou de uma (0,25%) a cinco (1,25%). Assim a detecção de S. sclerotiorum pelo método Neon-S2 pode ser otimizada com a incubação por 15 dias, considerando que, neste caso, a formação de escleródios próximos às sementes infectadas confirma a presença do patógeno e evita a leitura de falsos positivos. O método Neon-S2 aumentou a sensibilidade de detecção de S. sclerotiorum em lotes de sementes analisadas quando comparado ao método de rolo, porém, quando comparado com o método Neon S, sua média foi maior, mas a diferença não foi significativa.Universidade Federal de UberlândiaBRPrograma de Pós-graduação em AgronomiaCiências AgráriasUFUJuliatti, Fernando Cezarhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787141T6Tebaldi, Nilvanira Donizetehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4761482J2Martins, Juliana Araújo Santoshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4702239J1Figueiró, Adriana de Andradehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4775344J9Santos, Roberto Resende dos2016-06-22T18:31:07Z2015-11-112016-06-22T18:31:07Z2015-02-24info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfSANTOS, Roberto Resende dos. Detection of sclerotinia sclerotiorum in modified NEON-S medium in soybean and common beans seeds. 2015. 36 f. Dissertação (Mestrado em Ciências Agrárias) - Universidade Federal de Uberlândia, Uberlândia, 2015. DOI https://doi.org/10.14393/ufu.di.2015.95https://repositorio.ufu.br/handle/123456789/12219https://doi.org/10.14393/ufu.di.2015.95porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFUinstname:Universidade Federal de Uberlândia (UFU)instacron:UFU2021-09-20T19:23:44Zoai:repositorio.ufu.br:123456789/12219Repositório InstitucionalONGhttp://repositorio.ufu.br/oai/requestdiinf@dirbi.ufu.bropendoar:2021-09-20T19:23:44Repositório Institucional da UFU - Universidade Federal de Uberlândia (UFU)false
dc.title.none.fl_str_mv Detecção de Sclerotinia sclerotiorum em meio Neon-S modificado em sementes de soja e feijão
Detection of sclerotinia sclerotiorum in modified NEON-S medium in soybean and common beans seeds
title Detecção de Sclerotinia sclerotiorum em meio Neon-S modificado em sementes de soja e feijão
spellingShingle Detecção de Sclerotinia sclerotiorum em meio Neon-S modificado em sementes de soja e feijão
Santos, Roberto Resende dos
Glycine max
Phaseolus vulgaris
Sanidade de sementes
Mofo branco
Rolo de papel
Soja - Semente - Doenças e pragas
Feijão - Semente - Doenças e pragas
Sclerotinia sclerotiorum
Seed health
White mold
Paper roll
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA
title_short Detecção de Sclerotinia sclerotiorum em meio Neon-S modificado em sementes de soja e feijão
title_full Detecção de Sclerotinia sclerotiorum em meio Neon-S modificado em sementes de soja e feijão
title_fullStr Detecção de Sclerotinia sclerotiorum em meio Neon-S modificado em sementes de soja e feijão
title_full_unstemmed Detecção de Sclerotinia sclerotiorum em meio Neon-S modificado em sementes de soja e feijão
title_sort Detecção de Sclerotinia sclerotiorum em meio Neon-S modificado em sementes de soja e feijão
author Santos, Roberto Resende dos
author_facet Santos, Roberto Resende dos
author_role author
dc.contributor.none.fl_str_mv Juliatti, Fernando Cezar
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787141T6
Tebaldi, Nilvanira Donizete
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4761482J2
Martins, Juliana Araújo Santos
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4702239J1
Figueiró, Adriana de Andrade
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4775344J9
dc.contributor.author.fl_str_mv Santos, Roberto Resende dos
dc.subject.por.fl_str_mv Glycine max
Phaseolus vulgaris
Sanidade de sementes
Mofo branco
Rolo de papel
Soja - Semente - Doenças e pragas
Feijão - Semente - Doenças e pragas
Sclerotinia sclerotiorum
Seed health
White mold
Paper roll
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA
topic Glycine max
Phaseolus vulgaris
Sanidade de sementes
Mofo branco
Rolo de papel
Soja - Semente - Doenças e pragas
Feijão - Semente - Doenças e pragas
Sclerotinia sclerotiorum
Seed health
White mold
Paper roll
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA
description The Neon-S method has been used for the detection of Sclerotinia sclerotiorum in soybean and common beans since the agricultural year 2010. However, this method yields false positive readings due to other fungi that change medium pH. Thus, this study evaluated the contribution of increasing incubation period on the optimization of Neon-S reliability in different cultivars of soybeans and common beans. Nine treatments were evaluated in randomized blocks design, as a 3x3 factorial, with three replications. The factors were three detection methods (paper roll, Neon- S, and Neon S2) and three cultivars: naturally infected Pérola beans; naturally infected soybean cultivar BRS Valiosa RR, and artificially inoculated soybean cultivar Nidera 7255 RR). Inoculation of soybean Nidera 7255 RR was done by incubating the seeds for 72 h on PDA where the fungus was previously grown for seven days at 25 ºC. The three methods were compared, evaluating 400 seeds per replication, after incubation for seven days in Neon-S, for 15 days in Neon-S2, and for 30 days in paper roll, determining the presence of the fungus, and that of sclerotia adhered to the seeds. Twenty seeds were used per plate for media Neon-S and Neon-S2 (20 plates per replication), and 50 seeds per roll for the paper roll. Seeds were incubated at 20 ºC in BDO chamber in darkness. The data were submitted to the analysis of variance and the averages compared by the Tukey test at 5% probability. The interaction between detection method and cultivar was significant at 1% probability, indicating that the best method depends on the cultivar under study. Among the cultivars evaluated, artificially infected soybean presented the greatest indices of pathogen infection. A comparison of seed lots analyzed at the Plant Disease Clinic LAMIP proved greater sensibility of Neon-S in a total of 637 lots evaluated from 2008 to 2012. In that period, the paper roll test resulted in 21.88% positive samples, while in Neon-S 31.25% of them were positive. Another distinguishing factor among the lots analyzed was the percentage of infected and, or, contaminated seeds, which varied from one (0.25%) to five (1.25%). Therefore, detection of S. sclerotiorum by the Neon-S2 method can be optimized by incubation for 15 days, considering that, in this case, the formation of sclerotia near the infected seeds confirm the presence of the pathogen, avoiding false positive readings. Neon-S2 method increased detection sensibility of S. sclerotiorum in seed lots analyzed in comparison with the paper roll method; however, in comparison with Neon S method, despite its greater average, no significant differences were observed.
publishDate 2015
dc.date.none.fl_str_mv 2015-11-11
2015-02-24
2016-06-22T18:31:07Z
2016-06-22T18:31:07Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv SANTOS, Roberto Resende dos. Detection of sclerotinia sclerotiorum in modified NEON-S medium in soybean and common beans seeds. 2015. 36 f. Dissertação (Mestrado em Ciências Agrárias) - Universidade Federal de Uberlândia, Uberlândia, 2015. DOI https://doi.org/10.14393/ufu.di.2015.95
https://repositorio.ufu.br/handle/123456789/12219
https://doi.org/10.14393/ufu.di.2015.95
identifier_str_mv SANTOS, Roberto Resende dos. Detection of sclerotinia sclerotiorum in modified NEON-S medium in soybean and common beans seeds. 2015. 36 f. Dissertação (Mestrado em Ciências Agrárias) - Universidade Federal de Uberlândia, Uberlândia, 2015. DOI https://doi.org/10.14393/ufu.di.2015.95
url https://repositorio.ufu.br/handle/123456789/12219
https://doi.org/10.14393/ufu.di.2015.95
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Uberlândia
BR
Programa de Pós-graduação em Agronomia
Ciências Agrárias
UFU
publisher.none.fl_str_mv Universidade Federal de Uberlândia
BR
Programa de Pós-graduação em Agronomia
Ciências Agrárias
UFU
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFU
instname:Universidade Federal de Uberlândia (UFU)
instacron:UFU
instname_str Universidade Federal de Uberlândia (UFU)
instacron_str UFU
institution UFU
reponame_str Repositório Institucional da UFU
collection Repositório Institucional da UFU
repository.name.fl_str_mv Repositório Institucional da UFU - Universidade Federal de Uberlândia (UFU)
repository.mail.fl_str_mv diinf@dirbi.ufu.br
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