Avaliação da produção de biofilme por amostras clínicas de Acinetobacter baumannii do estado de Minas Gerais

• Autor(a) principal: Ribeiro, Juliana de Almeida
• Data de Publicação: 2019
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UFU
• Texto Completo: https://repositorio.ufu.br/handle/123456789/28014
• topic: Acinetobacter baumannii; Biofilme; CNPQ::CIENCIAS BIOLOGICAS

Acinetobacter baumannii is a growing problem in hospitalized individuals, particularly that immunocompromised. Infections caused by this microorganism are associated with higher morbidity, mortality, length of stay and costs. The clinical importance of A. baumannii is related to the difficult eradication, increased incidence of multiresistant specimens, as well as its ability to resist disinfection, to persist for a long time on surfaces and to produce biofilm, which contributes to its survival in both host and hospital environment. Thus, the present study aimed to evaluate and compare biofilm production in clinical samples of A. baumannii from different hospitals and regions of the state of Minas Gerais. For the study, 10 previously characterized clinical samples were randomly selected, belonging to the culture collection of the Molecular Microbiology Laboratory of the Federal University of Uberlandia. Biofilm production was evaluated by inert surface adhesion, viable cell count and biomass quantification by the crystal violet method. All samples evaluated could adhere to the unmodified polystyrene surface and were able to produce biofilm after 24 hours of incubation, regardless of clinical origin and resistance profile. Thus, the present study demonstrated that the presence of resistance factors and the ability to form biofilm in A. baumannnii may contribute to the emergence and dissemination of adaptive phenotypes in the hospital environment. Additionally, the association between biofilm production and multidrug resistance is certainly a limiting factor for the treatment of serious infections caused by this phenotype.