Inibição dos principais efeitos tóxicos causados por venenos animais pelo extrato vegetal de Casearia sylvestris (Flacourtiaceae)
Autor(a) principal: | |
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Data de Publicação: | 1998 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFU |
Texto Completo: | https://repositorio.ufu.br/handle/123456789/30383 http://doi.org/10.14393/ufu.di.1998.26 |
Resumo: | Snake venoms are constituted by complex mixtures of proteases, hemorrhagic factors and phospholipases that are responsible for the main effects of the envenomations such as: myotoxicity, hemorrhage, disturbs of blood coagulation and edema. Many plants have been used in popular medicine for the treatment of snakebite, but its use has generally been attributed to the superstition and there are few scientific knowledge related to the characteristics presented by these plants. The purpose of this study was to verify the inhibition of the Bothrops jararacussu, Bothrops neuwiedi pauloensis and Apis mellifera venoms main effects by Casearia sylvestris (Flacourtiaceae) extract, a typical plant from Brazilian Savannah. Initially, an acjueous extract was prepared from the leaves and the stem of this plant and this extract was incubated with the crude venom sample by one hour before the test. We realized some activities as Phospholipase A2, blood coagulation, fibrinogenolytic, hemorrhagic, myotoxic, edema and toxicity. For Phospholipase A2 activity the results indicate a high percentage of inhibition for all the venoms, where the statistical analysis revealed that these extract inhibited significantly and that the both extracts (stem and leaves) are equally efficient. In the coagulation activity the results were statistically analyzed by the test T and showed significant differences only to B.jararacussu venom incubated with Casearia sylvestris leaves (inhibition of 38/o), while for the other samples, no significant neutralization was recorded. When we tested the inhibition of the fibrinogenolytic activity, we found that the extract (leaves or stem) offered little or no protection for the fibrinogen ct chain. The inactivation of the hemorrhagic activity was determined by intradermic injections in mice. The hemorrhagic activity presented great inhibition for the stem and leaves extract, suggesting the efficiency of this extract for this activity. For myotoxic activity, the histological observations show fibers in perfect State for the samples incubated with the extract when compared to the those that contained only venom and they indicate the presence of the components able to neutralize the toxic effects. In the edema activity was verified that the edema was not inhibited by the extract of the leaves of Casearia sylvestris, but the edema produced by the sample incubated with the extract was lightly smaller than that provoked by the venom in the extract absence. Finally, for toxicity test, the Casearia sylvestris extract provoked an increase in the lifetime of the animais that received intraperitonials injections of 2DL50 (lethal dose) of Bothrops jararacussu and Bothrops neuwiedi pauloensis venoms when these were incubated with the stem or leaves extract. Despite all the animais died along the experiment, the lifetime average was prolonged and it showed significant results for Bothrops jararacussu venom. For all the activities, negative Controls were accomplished, using only the extracts and we can conclude that these extracts don't induce to any activity. In agreement with the results above, we verified that Casearia sylvestris (Flacourtiaceae) extract can neutralize animais venoms. The high inhibition percentages for the hemorrhagic, phospholipase A2 and myotoxic activities, indicate the presence of components that could present great therapeutic value for treatment of animal envenomations and perhaps to for another clinicai disorders. |
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Inibição dos principais efeitos tóxicos causados por venenos animais pelo extrato vegetal de Casearia sylvestris (Flacourtiaceae)Inhibition of the main toxic effects caused by animal poisons by the plant extract of Casearia sylvestris (Flacourtiaceae)Venenos animaisMiotoxicidadeAção enzimáticaCNPQ::CIENCIAS BIOLOGICASAnimais venenososSnake venoms are constituted by complex mixtures of proteases, hemorrhagic factors and phospholipases that are responsible for the main effects of the envenomations such as: myotoxicity, hemorrhage, disturbs of blood coagulation and edema. Many plants have been used in popular medicine for the treatment of snakebite, but its use has generally been attributed to the superstition and there are few scientific knowledge related to the characteristics presented by these plants. The purpose of this study was to verify the inhibition of the Bothrops jararacussu, Bothrops neuwiedi pauloensis and Apis mellifera venoms main effects by Casearia sylvestris (Flacourtiaceae) extract, a typical plant from Brazilian Savannah. Initially, an acjueous extract was prepared from the leaves and the stem of this plant and this extract was incubated with the crude venom sample by one hour before the test. We realized some activities as Phospholipase A2, blood coagulation, fibrinogenolytic, hemorrhagic, myotoxic, edema and toxicity. For Phospholipase A2 activity the results indicate a high percentage of inhibition for all the venoms, where the statistical analysis revealed that these extract inhibited significantly and that the both extracts (stem and leaves) are equally efficient. In the coagulation activity the results were statistically analyzed by the test T and showed significant differences only to B.jararacussu venom incubated with Casearia sylvestris leaves (inhibition of 38/o), while for the other samples, no significant neutralization was recorded. When we tested the inhibition of the fibrinogenolytic activity, we found that the extract (leaves or stem) offered little or no protection for the fibrinogen ct chain. The inactivation of the hemorrhagic activity was determined by intradermic injections in mice. The hemorrhagic activity presented great inhibition for the stem and leaves extract, suggesting the efficiency of this extract for this activity. For myotoxic activity, the histological observations show fibers in perfect State for the samples incubated with the extract when compared to the those that contained only venom and they indicate the presence of the components able to neutralize the toxic effects. In the edema activity was verified that the edema was not inhibited by the extract of the leaves of Casearia sylvestris, but the edema produced by the sample incubated with the extract was lightly smaller than that provoked by the venom in the extract absence. Finally, for toxicity test, the Casearia sylvestris extract provoked an increase in the lifetime of the animais that received intraperitonials injections of 2DL50 (lethal dose) of Bothrops jararacussu and Bothrops neuwiedi pauloensis venoms when these were incubated with the stem or leaves extract. Despite all the animais died along the experiment, the lifetime average was prolonged and it showed significant results for Bothrops jararacussu venom. For all the activities, negative Controls were accomplished, using only the extracts and we can conclude that these extracts don't induce to any activity. In agreement with the results above, we verified that Casearia sylvestris (Flacourtiaceae) extract can neutralize animais venoms. The high inhibition percentages for the hemorrhagic, phospholipase A2 and myotoxic activities, indicate the presence of components that could present great therapeutic value for treatment of animal envenomations and perhaps to for another clinicai disorders.Dissertação (Mestrado)Os venenos animais são constituídos por complexas misturas de toxinas de origem principalmente protéica, com ação enzimática ou não e que são responsáveis pelos principais efeitos dos envenenamentos tais como; miotoxicidade, hemorragia, distúrbios na coagulação sanguínea e edema. Muitas plantas têm sido usadas pelo homem para o tratamento de acidentes ofídicos, mas geralmente seus usos têm sido atribuídos à superstição, e poucos são os conhecimentos científicos relacionados às características apresentadas por estas plantas. O objetivo deste trabalho foi verificar a inibição dos principais efeitos dos venenos das serpentes Bothrops jararacussu, Bothrops neuwiedi pauloensis e da abelha Apis mellifera pelo extrato vegetal de Casearia sylvestris (Flacourtiaceae), uma típica planta do Cerrado Brasileiro. Inicialmente, foi preparado um extrato aquoso a partir das folhas e do caule desta planta. Para cada ensaio de inibição, o extrato foi incubado com o respectivo veneno por uma hora antes do teste. Foram realizados ensaios para as atividades fosfolipásica A2, coagulante, fibrinogenolítica, hemorrágica miotóxica, edematogênica e toxicidade. Para a atividade fosfolipásica A2, os resultados mostram uma alta porcentagem de inibição para todos os venenos, onde testes estatísticos revelaram a eficácia do extrato vegetal tanto para o caule quanto para as folhas. Na atividade coagulante, os resultados foram estatisticamente testados pelo teste T e mostraram que apenas B jararacussu incubado com Casearia sylvestris folhas, apresentou diferenças significativas (inibição de 38%), enquanto para as outras amostras, não houve inibição significativa. Quando testamos a inibição da atividade fibrinogenolítica, encontramos que o extrato (folhas ou caule) ofereceu pouca ou nenhuma proteção para a cadeia a do fibrinogênio. A neutralização da hemorragia foi determinada por injeções intradérmicas no dorso de camundongos, assim, a atividade hemorrágica apresentou grande inibição para o extrato do caule e das folhas, sugerindo a eficiência deste extrato para esta atividade. Para a atividade miotóxica, os bons resultados obtidos também sugerem a presença de componentes capazes de interferir no efeito tóxico dos venenos, pois os cortes histológicos mostram fibras em perfeito estado para as amostras incubadas com o extrato quando comparadas à aquelas que continham apenas veneno. Na atividade edematogênica, verificou-se que o edema formado pelo veneno de Bothrops jararacussu não foi inibido significativamente pelo extrato vegetal das folhas de Casearia sylvestris, mas em relação ao controle foi ligeiramente menor. Já para o teste de toxicidade o extrato vegetal de Casearia sylvestris provocou um aumento na sobrevida dos animais que receberam injeções intraperitoniais de 2DL50 (Dose letal 50%) dos venenos de Bothrops jararacussu e Bothrops neuwiedi pauloensis incubados com o extrato do caule ou das folhas. Embora todos os animais morrerem ao longo do experimento, verificou-se que o tempo médio de sobrevida foi prolongado. Para todas as atividades, foram realizados controles negativos, utilizando somente os extratos vegetais e podemos concluir que estes extratos não induzem a nenhuma atividade. De acordo com os resultados acima, verificamos o potencial inibitório do extrato de Casearia sylvestris (Flacourtiaceae). As altas porcentagens de inibição para as atividades hemorrágica, fosfolipásica A2 e miotóxica, indicam a presença de componentes que poderão apresentar grande valor terapêutico para tratamento de envenenamentos animais e talvez até para outras desordens clínicas.Universidade Federal de UberlândiaBrasilPrograma de Pós-graduação em Genética e BioquímicaBrandeburgo, Maria Inês Homsihttp://lattes.cnpq.br/671690758857962Borges, Márcia Helena2020-11-13T16:39:22Z2020-11-13T16:39:22Z1998info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfBORGES, Márcia Helena. Inibição dos principais efeitos tóxicos causados por venenos animais pelo extrato vegetal de Casearia sylvestris (flacourtiaceae). 1998. 71 f. Dissertação (Mestrado em Genética e Bioquímica) - Universidade Federal de Uberlândia, Uberlândia, 2020. DOI http://doi.org/10.14393/ufu.di.1998.26https://repositorio.ufu.br/handle/123456789/30383http://doi.org/10.14393/ufu.di.1998.26porhttp://creativecommons.org/licenses/by-nc-nd/3.0/us/info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFUinstname:Universidade Federal de Uberlândia (UFU)instacron:UFU2020-11-14T06:13:47Zoai:repositorio.ufu.br:123456789/30383Repositório InstitucionalONGhttp://repositorio.ufu.br/oai/requestdiinf@dirbi.ufu.bropendoar:2020-11-14T06:13:47Repositório Institucional da UFU - Universidade Federal de Uberlândia (UFU)false |
dc.title.none.fl_str_mv |
Inibição dos principais efeitos tóxicos causados por venenos animais pelo extrato vegetal de Casearia sylvestris (Flacourtiaceae) Inhibition of the main toxic effects caused by animal poisons by the plant extract of Casearia sylvestris (Flacourtiaceae) |
title |
Inibição dos principais efeitos tóxicos causados por venenos animais pelo extrato vegetal de Casearia sylvestris (Flacourtiaceae) |
spellingShingle |
Inibição dos principais efeitos tóxicos causados por venenos animais pelo extrato vegetal de Casearia sylvestris (Flacourtiaceae) Borges, Márcia Helena Venenos animais Miotoxicidade Ação enzimática CNPQ::CIENCIAS BIOLOGICAS Animais venenosos |
title_short |
Inibição dos principais efeitos tóxicos causados por venenos animais pelo extrato vegetal de Casearia sylvestris (Flacourtiaceae) |
title_full |
Inibição dos principais efeitos tóxicos causados por venenos animais pelo extrato vegetal de Casearia sylvestris (Flacourtiaceae) |
title_fullStr |
Inibição dos principais efeitos tóxicos causados por venenos animais pelo extrato vegetal de Casearia sylvestris (Flacourtiaceae) |
title_full_unstemmed |
Inibição dos principais efeitos tóxicos causados por venenos animais pelo extrato vegetal de Casearia sylvestris (Flacourtiaceae) |
title_sort |
Inibição dos principais efeitos tóxicos causados por venenos animais pelo extrato vegetal de Casearia sylvestris (Flacourtiaceae) |
author |
Borges, Márcia Helena |
author_facet |
Borges, Márcia Helena |
author_role |
author |
dc.contributor.none.fl_str_mv |
Brandeburgo, Maria Inês Homsi http://lattes.cnpq.br/671690758857962 |
dc.contributor.author.fl_str_mv |
Borges, Márcia Helena |
dc.subject.por.fl_str_mv |
Venenos animais Miotoxicidade Ação enzimática CNPQ::CIENCIAS BIOLOGICAS Animais venenosos |
topic |
Venenos animais Miotoxicidade Ação enzimática CNPQ::CIENCIAS BIOLOGICAS Animais venenosos |
description |
Snake venoms are constituted by complex mixtures of proteases, hemorrhagic factors and phospholipases that are responsible for the main effects of the envenomations such as: myotoxicity, hemorrhage, disturbs of blood coagulation and edema. Many plants have been used in popular medicine for the treatment of snakebite, but its use has generally been attributed to the superstition and there are few scientific knowledge related to the characteristics presented by these plants. The purpose of this study was to verify the inhibition of the Bothrops jararacussu, Bothrops neuwiedi pauloensis and Apis mellifera venoms main effects by Casearia sylvestris (Flacourtiaceae) extract, a typical plant from Brazilian Savannah. Initially, an acjueous extract was prepared from the leaves and the stem of this plant and this extract was incubated with the crude venom sample by one hour before the test. We realized some activities as Phospholipase A2, blood coagulation, fibrinogenolytic, hemorrhagic, myotoxic, edema and toxicity. For Phospholipase A2 activity the results indicate a high percentage of inhibition for all the venoms, where the statistical analysis revealed that these extract inhibited significantly and that the both extracts (stem and leaves) are equally efficient. In the coagulation activity the results were statistically analyzed by the test T and showed significant differences only to B.jararacussu venom incubated with Casearia sylvestris leaves (inhibition of 38/o), while for the other samples, no significant neutralization was recorded. When we tested the inhibition of the fibrinogenolytic activity, we found that the extract (leaves or stem) offered little or no protection for the fibrinogen ct chain. The inactivation of the hemorrhagic activity was determined by intradermic injections in mice. The hemorrhagic activity presented great inhibition for the stem and leaves extract, suggesting the efficiency of this extract for this activity. For myotoxic activity, the histological observations show fibers in perfect State for the samples incubated with the extract when compared to the those that contained only venom and they indicate the presence of the components able to neutralize the toxic effects. In the edema activity was verified that the edema was not inhibited by the extract of the leaves of Casearia sylvestris, but the edema produced by the sample incubated with the extract was lightly smaller than that provoked by the venom in the extract absence. Finally, for toxicity test, the Casearia sylvestris extract provoked an increase in the lifetime of the animais that received intraperitonials injections of 2DL50 (lethal dose) of Bothrops jararacussu and Bothrops neuwiedi pauloensis venoms when these were incubated with the stem or leaves extract. Despite all the animais died along the experiment, the lifetime average was prolonged and it showed significant results for Bothrops jararacussu venom. For all the activities, negative Controls were accomplished, using only the extracts and we can conclude that these extracts don't induce to any activity. In agreement with the results above, we verified that Casearia sylvestris (Flacourtiaceae) extract can neutralize animais venoms. The high inhibition percentages for the hemorrhagic, phospholipase A2 and myotoxic activities, indicate the presence of components that could present great therapeutic value for treatment of animal envenomations and perhaps to for another clinicai disorders. |
publishDate |
1998 |
dc.date.none.fl_str_mv |
1998 2020-11-13T16:39:22Z 2020-11-13T16:39:22Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
BORGES, Márcia Helena. Inibição dos principais efeitos tóxicos causados por venenos animais pelo extrato vegetal de Casearia sylvestris (flacourtiaceae). 1998. 71 f. Dissertação (Mestrado em Genética e Bioquímica) - Universidade Federal de Uberlândia, Uberlândia, 2020. DOI http://doi.org/10.14393/ufu.di.1998.26 https://repositorio.ufu.br/handle/123456789/30383 http://doi.org/10.14393/ufu.di.1998.26 |
identifier_str_mv |
BORGES, Márcia Helena. Inibição dos principais efeitos tóxicos causados por venenos animais pelo extrato vegetal de Casearia sylvestris (flacourtiaceae). 1998. 71 f. Dissertação (Mestrado em Genética e Bioquímica) - Universidade Federal de Uberlândia, Uberlândia, 2020. DOI http://doi.org/10.14393/ufu.di.1998.26 |
url |
https://repositorio.ufu.br/handle/123456789/30383 http://doi.org/10.14393/ufu.di.1998.26 |
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por |
language |
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http://creativecommons.org/licenses/by-nc-nd/3.0/us/ info:eu-repo/semantics/openAccess |
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http://creativecommons.org/licenses/by-nc-nd/3.0/us/ |
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openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Uberlândia Brasil Programa de Pós-graduação em Genética e Bioquímica |
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Universidade Federal de Uberlândia Brasil Programa de Pós-graduação em Genética e Bioquímica |
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reponame:Repositório Institucional da UFU instname:Universidade Federal de Uberlândia (UFU) instacron:UFU |
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Universidade Federal de Uberlândia (UFU) |
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Repositório Institucional da UFU - Universidade Federal de Uberlândia (UFU) |
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diinf@dirbi.ufu.br |
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