Evaluation of magnetic carriers employed for immobilization of lipase from candida rugosa
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista de Engenharia Química e Química |
Texto Completo: | https://periodicos.ufv.br/jcec/article/view/10858 |
Resumo: | Currently, the use of magnetic nanoparticles has aroused interest in industrial processes, and the combination of their properties with the immobilization of lipases has been developed in order to produce carriers of easy separation of the reaction medium. In this context, the objective of the present study was to immobilize Candida rugosa lipase in magnetic nanoparticles, such as magnetite and maghemite, by physical adsorption and covalent bonding. The biocatalysts were evaluated by infrared spectroscopy (FTIR) and hydrolytic activity analysis. Thus, from the analyses performed, the best biocatalyst obtained was the immobilized by covalent bond in maghemite, presenting a hydrolytic activity of 174.67 U/g. |
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Revista de Engenharia Química e Química |
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Evaluation of magnetic carriers employed for immobilization of lipase from candida rugosa Avaliação de suportes magnéticos utilizados para imobilização da lipase de candida rugosaInfrared spectroscopyEnzyme immobilizationCovalent bondingMaghemiteMagnetic nanoparticlesEspectroscopia de infravermelhoImobilização de enzimasLigação CovalenteMaghemitaNanopartículas magnéticasCurrently, the use of magnetic nanoparticles has aroused interest in industrial processes, and the combination of their properties with the immobilization of lipases has been developed in order to produce carriers of easy separation of the reaction medium. In this context, the objective of the present study was to immobilize Candida rugosa lipase in magnetic nanoparticles, such as magnetite and maghemite, by physical adsorption and covalent bonding. The biocatalysts were evaluated by infrared spectroscopy (FTIR) and hydrolytic activity analysis. Thus, from the analyses performed, the best biocatalyst obtained was the immobilized by covalent bond in maghemite, presenting a hydrolytic activity of 174.67 U/g.Atualmente, o uso de nanopartículas magnéticas tem despertado interesse em processos industriais, e a combinação de suas propriedades com a imobilização de lipases tem sido desenvolvida a fim de produzir suportes de fácil separação do meio reacional. Nesse contexto, o objetivo do presente estudo foi imobilizar a lipase de Candida rugosa em nanopartículas magnéticas, como magnetita e maghemita, por adsorção física e ligação covalente. Os biocatalicadores foram avaliados por espectroscopia de infravermelho (FTIR) e análise de atividade hidrolítica. Dessa forma, a partir das análises realizadas, o melhor biocatalisador obtido foi o imobilizado por ligação covalente em maghemita, apresentando atividade hidrolítica de 174,67 U/g.Universidade Federal de Viçosa - UFV2020-10-21info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.ufv.br/jcec/article/view/1085810.18540/jcecvl6iss4pp0498-0504The Journal of Engineering and Exact Sciences; Vol. 6 No. 4 (2020); 0498-0504The Journal of Engineering and Exact Sciences; Vol. 6 Núm. 4 (2020); 0498-0504The Journal of Engineering and Exact Sciences; v. 6 n. 4 (2020); 0498-05042527-1075reponame:Revista de Engenharia Química e Químicainstname:Universidade Federal de Viçosa (UFV)instacron:UFVenghttps://periodicos.ufv.br/jcec/article/view/10858/6060Copyright (c) 2020 The Journal of Engineering and Exact Scienceshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessDomingues, OtávioSantos, Letícia Karen dosHerculano, Rondinelli DonizettiFlumignan, Danilo LuizPaula, Ariela Veloso de2021-02-23T20:11:08Zoai:ojs.periodicos.ufv.br:article/10858Revistahttp://www.seer.ufv.br/seer/rbeq2/index.php/req2/indexONGhttps://periodicos.ufv.br/jcec/oaijcec.journal@ufv.br||req2@ufv.br2446-94162446-9416opendoar:2021-02-23T20:11:08Revista de Engenharia Química e Química - Universidade Federal de Viçosa (UFV)false |
dc.title.none.fl_str_mv |
Evaluation of magnetic carriers employed for immobilization of lipase from candida rugosa Avaliação de suportes magnéticos utilizados para imobilização da lipase de candida rugosa |
title |
Evaluation of magnetic carriers employed for immobilization of lipase from candida rugosa |
spellingShingle |
Evaluation of magnetic carriers employed for immobilization of lipase from candida rugosa Domingues, Otávio Infrared spectroscopy Enzyme immobilization Covalent bonding Maghemite Magnetic nanoparticles Espectroscopia de infravermelho Imobilização de enzimas Ligação Covalente Maghemita Nanopartículas magnéticas |
title_short |
Evaluation of magnetic carriers employed for immobilization of lipase from candida rugosa |
title_full |
Evaluation of magnetic carriers employed for immobilization of lipase from candida rugosa |
title_fullStr |
Evaluation of magnetic carriers employed for immobilization of lipase from candida rugosa |
title_full_unstemmed |
Evaluation of magnetic carriers employed for immobilization of lipase from candida rugosa |
title_sort |
Evaluation of magnetic carriers employed for immobilization of lipase from candida rugosa |
author |
Domingues, Otávio |
author_facet |
Domingues, Otávio Santos, Letícia Karen dos Herculano, Rondinelli Donizetti Flumignan, Danilo Luiz Paula, Ariela Veloso de |
author_role |
author |
author2 |
Santos, Letícia Karen dos Herculano, Rondinelli Donizetti Flumignan, Danilo Luiz Paula, Ariela Veloso de |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Domingues, Otávio Santos, Letícia Karen dos Herculano, Rondinelli Donizetti Flumignan, Danilo Luiz Paula, Ariela Veloso de |
dc.subject.por.fl_str_mv |
Infrared spectroscopy Enzyme immobilization Covalent bonding Maghemite Magnetic nanoparticles Espectroscopia de infravermelho Imobilização de enzimas Ligação Covalente Maghemita Nanopartículas magnéticas |
topic |
Infrared spectroscopy Enzyme immobilization Covalent bonding Maghemite Magnetic nanoparticles Espectroscopia de infravermelho Imobilização de enzimas Ligação Covalente Maghemita Nanopartículas magnéticas |
description |
Currently, the use of magnetic nanoparticles has aroused interest in industrial processes, and the combination of their properties with the immobilization of lipases has been developed in order to produce carriers of easy separation of the reaction medium. In this context, the objective of the present study was to immobilize Candida rugosa lipase in magnetic nanoparticles, such as magnetite and maghemite, by physical adsorption and covalent bonding. The biocatalysts were evaluated by infrared spectroscopy (FTIR) and hydrolytic activity analysis. Thus, from the analyses performed, the best biocatalyst obtained was the immobilized by covalent bond in maghemite, presenting a hydrolytic activity of 174.67 U/g. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-10-21 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.ufv.br/jcec/article/view/10858 10.18540/jcecvl6iss4pp0498-0504 |
url |
https://periodicos.ufv.br/jcec/article/view/10858 |
identifier_str_mv |
10.18540/jcecvl6iss4pp0498-0504 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://periodicos.ufv.br/jcec/article/view/10858/6060 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2020 The Journal of Engineering and Exact Sciences https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2020 The Journal of Engineering and Exact Sciences https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Viçosa - UFV |
publisher.none.fl_str_mv |
Universidade Federal de Viçosa - UFV |
dc.source.none.fl_str_mv |
The Journal of Engineering and Exact Sciences; Vol. 6 No. 4 (2020); 0498-0504 The Journal of Engineering and Exact Sciences; Vol. 6 Núm. 4 (2020); 0498-0504 The Journal of Engineering and Exact Sciences; v. 6 n. 4 (2020); 0498-0504 2527-1075 reponame:Revista de Engenharia Química e Química instname:Universidade Federal de Viçosa (UFV) instacron:UFV |
instname_str |
Universidade Federal de Viçosa (UFV) |
instacron_str |
UFV |
institution |
UFV |
reponame_str |
Revista de Engenharia Química e Química |
collection |
Revista de Engenharia Química e Química |
repository.name.fl_str_mv |
Revista de Engenharia Química e Química - Universidade Federal de Viçosa (UFV) |
repository.mail.fl_str_mv |
jcec.journal@ufv.br||req2@ufv.br |
_version_ |
1800211189455650816 |