Evaluation of magnetic carriers employed for immobilization of lipase from candida rugosa

Detalhes bibliográficos
Autor(a) principal: Domingues, Otávio
Data de Publicação: 2020
Outros Autores: Santos, Letícia Karen dos, Herculano, Rondinelli Donizetti, Flumignan, Danilo Luiz, Paula, Ariela Veloso de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: The Journal of Engineering and Exact Sciences
Texto Completo: https://periodicos.ufv.br/jcec/article/view/10858
Resumo: Currently, the use of magnetic nanoparticles has aroused interest in industrial processes, and the combination of their properties with the immobilization of lipases has been developed in order to produce carriers of easy separation of the reaction medium. In this context, the objective of the present study was to immobilize Candida rugosa lipase in magnetic nanoparticles, such as magnetite and maghemite, by physical adsorption and covalent bonding. The biocatalysts were evaluated by infrared spectroscopy (FTIR) and hydrolytic activity analysis. Thus, from the analyses performed, the best biocatalyst obtained was the immobilized by covalent bond in maghemite, presenting a hydrolytic activity of 174.67 U/g.
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spelling Evaluation of magnetic carriers employed for immobilization of lipase from candida rugosa Avaliação de suportes magnéticos utilizados para imobilização da lipase de candida rugosaInfrared spectroscopyEnzyme immobilizationCovalent bondingMaghemiteMagnetic nanoparticlesEspectroscopia de infravermelhoImobilização de enzimasLigação CovalenteMaghemitaNanopartículas magnéticasCurrently, the use of magnetic nanoparticles has aroused interest in industrial processes, and the combination of their properties with the immobilization of lipases has been developed in order to produce carriers of easy separation of the reaction medium. In this context, the objective of the present study was to immobilize Candida rugosa lipase in magnetic nanoparticles, such as magnetite and maghemite, by physical adsorption and covalent bonding. The biocatalysts were evaluated by infrared spectroscopy (FTIR) and hydrolytic activity analysis. Thus, from the analyses performed, the best biocatalyst obtained was the immobilized by covalent bond in maghemite, presenting a hydrolytic activity of 174.67 U/g.Atualmente, o uso de nanopartículas magnéticas tem despertado interesse em processos industriais, e a combinação de suas propriedades com a imobilização de lipases tem sido desenvolvida a fim de produzir suportes de fácil separação do meio reacional. Nesse contexto, o objetivo do presente estudo foi imobilizar a lipase de Candida rugosa em nanopartículas magnéticas, como magnetita e maghemita, por adsorção física e ligação covalente. Os biocatalicadores foram avaliados por espectroscopia de infravermelho (FTIR) e análise de atividade hidrolítica. Dessa forma, a partir das análises realizadas, o melhor biocatalisador obtido foi o imobilizado por ligação covalente em maghemita, apresentando atividade hidrolítica de 174,67 U/g.Universidade Federal de Viçosa - UFV2020-10-21info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.ufv.br/jcec/article/view/1085810.18540/jcecvl6iss4pp0498-0504The Journal of Engineering and Exact Sciences; Vol. 6 No. 4 (2020); 0498-0504The Journal of Engineering and Exact Sciences; Vol. 6 Núm. 4 (2020); 0498-0504The Journal of Engineering and Exact Sciences; v. 6 n. 4 (2020); 0498-05042527-1075reponame:The Journal of Engineering and Exact Sciencesinstname:Universidade Federal de Viçosa (UFV)instacron:UFVenghttps://periodicos.ufv.br/jcec/article/view/10858/6060Copyright (c) 2020 The Journal of Engineering and Exact Scienceshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessDomingues, OtávioSantos, Letícia Karen dosHerculano, Rondinelli DonizettiFlumignan, Danilo LuizPaula, Ariela Veloso de2021-02-23T20:11:08Zoai:ojs.periodicos.ufv.br:article/10858Revistahttp://www.seer.ufv.br/seer/rbeq2/index.php/req2/oai2527-10752527-1075opendoar:2021-02-23T20:11:08The Journal of Engineering and Exact Sciences - Universidade Federal de Viçosa (UFV)false
dc.title.none.fl_str_mv Evaluation of magnetic carriers employed for immobilization of lipase from candida rugosa
Avaliação de suportes magnéticos utilizados para imobilização da lipase de candida rugosa
title Evaluation of magnetic carriers employed for immobilization of lipase from candida rugosa
spellingShingle Evaluation of magnetic carriers employed for immobilization of lipase from candida rugosa
Domingues, Otávio
Infrared spectroscopy
Enzyme immobilization
Covalent bonding
Maghemite
Magnetic nanoparticles
Espectroscopia de infravermelho
Imobilização de enzimas
Ligação Covalente
Maghemita
Nanopartículas magnéticas
title_short Evaluation of magnetic carriers employed for immobilization of lipase from candida rugosa
title_full Evaluation of magnetic carriers employed for immobilization of lipase from candida rugosa
title_fullStr Evaluation of magnetic carriers employed for immobilization of lipase from candida rugosa
title_full_unstemmed Evaluation of magnetic carriers employed for immobilization of lipase from candida rugosa
title_sort Evaluation of magnetic carriers employed for immobilization of lipase from candida rugosa
author Domingues, Otávio
author_facet Domingues, Otávio
Santos, Letícia Karen dos
Herculano, Rondinelli Donizetti
Flumignan, Danilo Luiz
Paula, Ariela Veloso de
author_role author
author2 Santos, Letícia Karen dos
Herculano, Rondinelli Donizetti
Flumignan, Danilo Luiz
Paula, Ariela Veloso de
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Domingues, Otávio
Santos, Letícia Karen dos
Herculano, Rondinelli Donizetti
Flumignan, Danilo Luiz
Paula, Ariela Veloso de
dc.subject.por.fl_str_mv Infrared spectroscopy
Enzyme immobilization
Covalent bonding
Maghemite
Magnetic nanoparticles
Espectroscopia de infravermelho
Imobilização de enzimas
Ligação Covalente
Maghemita
Nanopartículas magnéticas
topic Infrared spectroscopy
Enzyme immobilization
Covalent bonding
Maghemite
Magnetic nanoparticles
Espectroscopia de infravermelho
Imobilização de enzimas
Ligação Covalente
Maghemita
Nanopartículas magnéticas
description Currently, the use of magnetic nanoparticles has aroused interest in industrial processes, and the combination of their properties with the immobilization of lipases has been developed in order to produce carriers of easy separation of the reaction medium. In this context, the objective of the present study was to immobilize Candida rugosa lipase in magnetic nanoparticles, such as magnetite and maghemite, by physical adsorption and covalent bonding. The biocatalysts were evaluated by infrared spectroscopy (FTIR) and hydrolytic activity analysis. Thus, from the analyses performed, the best biocatalyst obtained was the immobilized by covalent bond in maghemite, presenting a hydrolytic activity of 174.67 U/g.
publishDate 2020
dc.date.none.fl_str_mv 2020-10-21
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.ufv.br/jcec/article/view/10858
10.18540/jcecvl6iss4pp0498-0504
url https://periodicos.ufv.br/jcec/article/view/10858
identifier_str_mv 10.18540/jcecvl6iss4pp0498-0504
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://periodicos.ufv.br/jcec/article/view/10858/6060
dc.rights.driver.fl_str_mv Copyright (c) 2020 The Journal of Engineering and Exact Sciences
https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2020 The Journal of Engineering and Exact Sciences
https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Viçosa - UFV
publisher.none.fl_str_mv Universidade Federal de Viçosa - UFV
dc.source.none.fl_str_mv The Journal of Engineering and Exact Sciences; Vol. 6 No. 4 (2020); 0498-0504
The Journal of Engineering and Exact Sciences; Vol. 6 Núm. 4 (2020); 0498-0504
The Journal of Engineering and Exact Sciences; v. 6 n. 4 (2020); 0498-0504
2527-1075
reponame:The Journal of Engineering and Exact Sciences
instname:Universidade Federal de Viçosa (UFV)
instacron:UFV
instname_str Universidade Federal de Viçosa (UFV)
instacron_str UFV
institution UFV
reponame_str The Journal of Engineering and Exact Sciences
collection The Journal of Engineering and Exact Sciences
repository.name.fl_str_mv The Journal of Engineering and Exact Sciences - Universidade Federal de Viçosa (UFV)
repository.mail.fl_str_mv
_version_ 1808845246032773120

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