Production of beta-D-fructofuranosidases by Aspergillus sp. M2.4 and biochemical characterization
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Revista de Engenharia Química e Química |
Texto Completo: | https://periodicos.ufv.br/jcec/article/view/15943 |
Resumo: | Microbial enzymes, such as beta-D-fructofuranosidases that catalyze the hydrolysis of sucrose, are a clean alternative of catalysts for industries that manufacture better quality consumables, as they have a high degree of specificity, allow the regulation of activity and are synthesized under simple cultivation conditions. In this work, the production of invertases from the filamentous fungus Aspergillus sp. M2.4, as well as the biochemical characterization, since each process depends on specific parameters, and each microorganism synthesizes invertases with certain characteristics. To this end, filamentous fungi were isolated from decomposing organic matter, which were not good producers of invertase. Thus, four filamentous fungi from the laboratory's fungal bank were analyzed, selecting Aspergillus sp. M2.4, having as the best standards the growth in submerged culture medium Carvalho-Peixoto, for five days, at 30ºC, in a stationary way, using wheat bran as a carbon source, and yeast extract as a nitrogen source, as well as the CP medium salt solution itself, which provided an increase in activity from 35 total U to 290 total U, representing an 8-fold increase. In addition, the produced invertase showed an optimal point at pH 4.5 and 65ºC, which provided an increase in activity for more than 400 total U, about 12 times higher. It is worth mentioning that the enzyme showed good stability at pH, maintaining activity greater than 69% at pH 4.5 to 7.0, for 120 minutes, in addition to excellent thermostability, at temperatures from 50ºC to 55°C, with activity residual greater than 80%. These results suggest a possible industrial application of the invertase studied in bioprocesses, focusing on the reduction of sucrose crystallization in the production of ethanol from sugarcane and the synthesis of fructooligosaccharides. |
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Production of beta-D-fructofuranosidases by Aspergillus sp. M2.4 and biochemical characterizationProdução de beta-D-frutofuranosidases por Aspergillus sp. M2.4 e caracterização bioquímicaAspergillusBioprospectingEnzymesFilamentous fungiInvertaseAspergillusBioprospecçãoEnzimasFungos FilamentososInvertaseMicrobial enzymes, such as beta-D-fructofuranosidases that catalyze the hydrolysis of sucrose, are a clean alternative of catalysts for industries that manufacture better quality consumables, as they have a high degree of specificity, allow the regulation of activity and are synthesized under simple cultivation conditions. In this work, the production of invertases from the filamentous fungus Aspergillus sp. M2.4, as well as the biochemical characterization, since each process depends on specific parameters, and each microorganism synthesizes invertases with certain characteristics. To this end, filamentous fungi were isolated from decomposing organic matter, which were not good producers of invertase. Thus, four filamentous fungi from the laboratory's fungal bank were analyzed, selecting Aspergillus sp. M2.4, having as the best standards the growth in submerged culture medium Carvalho-Peixoto, for five days, at 30ºC, in a stationary way, using wheat bran as a carbon source, and yeast extract as a nitrogen source, as well as the CP medium salt solution itself, which provided an increase in activity from 35 total U to 290 total U, representing an 8-fold increase. In addition, the produced invertase showed an optimal point at pH 4.5 and 65ºC, which provided an increase in activity for more than 400 total U, about 12 times higher. It is worth mentioning that the enzyme showed good stability at pH, maintaining activity greater than 69% at pH 4.5 to 7.0, for 120 minutes, in addition to excellent thermostability, at temperatures from 50ºC to 55°C, with activity residual greater than 80%. These results suggest a possible industrial application of the invertase studied in bioprocesses, focusing on the reduction of sucrose crystallization in the production of ethanol from sugarcane and the synthesis of fructooligosaccharides.As enzimas microbianas, como as beta-D-frutofuranosidases catalisadoras da hidrolise da sacarose, são uma alternativa limpa de catalisadores para as indústrias de fabricação de bens consumíveis de melhor qualidade, pois apresentam um alto grau de especificidade, permitem a regulação da atividade e são sintetizadas em condições simples de cultivo. Neste trabalho foi estudada a produção de invertases a partir do fungo filamentoso Aspergillus sp. M2.4, bem como a caracterização bioquímica, uma vez que cada processo depende de parâmetros específicos, e cada micro-organismo sintetiza invertases com determinadas características. Para tal, realizou-se o isolamento de fungos filamentosos de matéria orgânica em decomposição, os quais não apresentaram como bons produtores da invertase. Dessa forma, analisou-se quatro fungos filamentosos do banco de fungos do laboratório, selecionando-se o Aspergillus sp. M2.4, tendo como melhores padrões o crescimento em meio de cultura submerso Carvalho-Peixoto, durante cinco dias, a 30ºC, de forma estacionária, utilizando-se como fonte de carbono o farelo de trigo, e fonte de nitrogênio o extrato de levedura, assim como a própria solução de sais do meio CP, os quais proporcionaram um aumento da atividade de 35 U totais para 290 U totais, representando um aumento de 8 vezes. Além disso, a invertase produzida apresentou ponto ótimo no pH 4.5 e a 65ºC, o qual proporcionou um aumento na atividade para mais de 400 U totais, superior cerca de 12 vezes. Vale citar que, a enzima apesentou boa estabilidade ao pH, mantendo atividade superior a 69% nos pH de 4.5 a 7.0, durante 120 minutos, além de uma excelente termoestabilidade, nas temperaturas de 50ºC a 55°C, com atividade residual superior a 80%. Esses resultados sugerem uma possível aplicação industrial da invertase estudada em bioprocessos, tendo como foco a redução da cristalização da sacarose na produção do etanol da cana-de-açúcar e síntese de frutooligossacarídeos.Universidade Federal de Viçosa - UFV2023-06-21info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.ufv.br/jcec/article/view/1594310.18540/jcecvl9iss5pp15943-01eThe Journal of Engineering and Exact Sciences; Vol. 9 No. 5 (2023); 15943-01eThe Journal of Engineering and Exact Sciences; Vol. 9 Núm. 5 (2023); 15943-01eThe Journal of Engineering and Exact Sciences; v. 9 n. 5 (2023); 15943-01e2527-1075reponame:Revista de Engenharia Química e Químicainstname:Universidade Federal de Viçosa (UFV)instacron:UFVporhttps://periodicos.ufv.br/jcec/article/view/15943/8020Copyright (c) 2023 The Journal of Engineering and Exact Scienceshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSilva , Mayanne TatielleLopes, Paulo Henrique SilvaPasin, Thiago MachadoNelson, David LeeBenassi, Vivian Machado2023-06-27T12:44:31Zoai:ojs.periodicos.ufv.br:article/15943Revistahttp://www.seer.ufv.br/seer/rbeq2/index.php/req2/indexONGhttps://periodicos.ufv.br/jcec/oaijcec.journal@ufv.br||req2@ufv.br2446-94162446-9416opendoar:2023-06-27T12:44:31Revista de Engenharia Química e Química - Universidade Federal de Viçosa (UFV)false |
dc.title.none.fl_str_mv |
Production of beta-D-fructofuranosidases by Aspergillus sp. M2.4 and biochemical characterization Produção de beta-D-frutofuranosidases por Aspergillus sp. M2.4 e caracterização bioquímica |
title |
Production of beta-D-fructofuranosidases by Aspergillus sp. M2.4 and biochemical characterization |
spellingShingle |
Production of beta-D-fructofuranosidases by Aspergillus sp. M2.4 and biochemical characterization Silva , Mayanne Tatielle Aspergillus Bioprospecting Enzymes Filamentous fungi Invertase Aspergillus Bioprospecção Enzimas Fungos Filamentosos Invertase |
title_short |
Production of beta-D-fructofuranosidases by Aspergillus sp. M2.4 and biochemical characterization |
title_full |
Production of beta-D-fructofuranosidases by Aspergillus sp. M2.4 and biochemical characterization |
title_fullStr |
Production of beta-D-fructofuranosidases by Aspergillus sp. M2.4 and biochemical characterization |
title_full_unstemmed |
Production of beta-D-fructofuranosidases by Aspergillus sp. M2.4 and biochemical characterization |
title_sort |
Production of beta-D-fructofuranosidases by Aspergillus sp. M2.4 and biochemical characterization |
author |
Silva , Mayanne Tatielle |
author_facet |
Silva , Mayanne Tatielle Lopes, Paulo Henrique Silva Pasin, Thiago Machado Nelson, David Lee Benassi, Vivian Machado |
author_role |
author |
author2 |
Lopes, Paulo Henrique Silva Pasin, Thiago Machado Nelson, David Lee Benassi, Vivian Machado |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Silva , Mayanne Tatielle Lopes, Paulo Henrique Silva Pasin, Thiago Machado Nelson, David Lee Benassi, Vivian Machado |
dc.subject.por.fl_str_mv |
Aspergillus Bioprospecting Enzymes Filamentous fungi Invertase Aspergillus Bioprospecção Enzimas Fungos Filamentosos Invertase |
topic |
Aspergillus Bioprospecting Enzymes Filamentous fungi Invertase Aspergillus Bioprospecção Enzimas Fungos Filamentosos Invertase |
description |
Microbial enzymes, such as beta-D-fructofuranosidases that catalyze the hydrolysis of sucrose, are a clean alternative of catalysts for industries that manufacture better quality consumables, as they have a high degree of specificity, allow the regulation of activity and are synthesized under simple cultivation conditions. In this work, the production of invertases from the filamentous fungus Aspergillus sp. M2.4, as well as the biochemical characterization, since each process depends on specific parameters, and each microorganism synthesizes invertases with certain characteristics. To this end, filamentous fungi were isolated from decomposing organic matter, which were not good producers of invertase. Thus, four filamentous fungi from the laboratory's fungal bank were analyzed, selecting Aspergillus sp. M2.4, having as the best standards the growth in submerged culture medium Carvalho-Peixoto, for five days, at 30ºC, in a stationary way, using wheat bran as a carbon source, and yeast extract as a nitrogen source, as well as the CP medium salt solution itself, which provided an increase in activity from 35 total U to 290 total U, representing an 8-fold increase. In addition, the produced invertase showed an optimal point at pH 4.5 and 65ºC, which provided an increase in activity for more than 400 total U, about 12 times higher. It is worth mentioning that the enzyme showed good stability at pH, maintaining activity greater than 69% at pH 4.5 to 7.0, for 120 minutes, in addition to excellent thermostability, at temperatures from 50ºC to 55°C, with activity residual greater than 80%. These results suggest a possible industrial application of the invertase studied in bioprocesses, focusing on the reduction of sucrose crystallization in the production of ethanol from sugarcane and the synthesis of fructooligosaccharides. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-06-21 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.ufv.br/jcec/article/view/15943 10.18540/jcecvl9iss5pp15943-01e |
url |
https://periodicos.ufv.br/jcec/article/view/15943 |
identifier_str_mv |
10.18540/jcecvl9iss5pp15943-01e |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://periodicos.ufv.br/jcec/article/view/15943/8020 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2023 The Journal of Engineering and Exact Sciences https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2023 The Journal of Engineering and Exact Sciences https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Viçosa - UFV |
publisher.none.fl_str_mv |
Universidade Federal de Viçosa - UFV |
dc.source.none.fl_str_mv |
The Journal of Engineering and Exact Sciences; Vol. 9 No. 5 (2023); 15943-01e The Journal of Engineering and Exact Sciences; Vol. 9 Núm. 5 (2023); 15943-01e The Journal of Engineering and Exact Sciences; v. 9 n. 5 (2023); 15943-01e 2527-1075 reponame:Revista de Engenharia Química e Química instname:Universidade Federal de Viçosa (UFV) instacron:UFV |
instname_str |
Universidade Federal de Viçosa (UFV) |
instacron_str |
UFV |
institution |
UFV |
reponame_str |
Revista de Engenharia Química e Química |
collection |
Revista de Engenharia Química e Química |
repository.name.fl_str_mv |
Revista de Engenharia Química e Química - Universidade Federal de Viçosa (UFV) |
repository.mail.fl_str_mv |
jcec.journal@ufv.br||req2@ufv.br |
_version_ |
1800211191240327168 |