Exogenous induction of ovarian activity and ovulation and transfer of fresh embryos of domestic cat (Felis catus)
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Ceres |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0034-737X2012000400010 |
Resumo: | The objective of the present study was the exogenous stimulation of ovarian activity and definition of embryo collection, and transfer protocols, in the domestic cat for potential application in non-domestic endangered species. Sixteen adult queens and two adult male reproducers kept in the experimental cat house at the Morphology sector at the Veterinary Department (DVT), UFV, were used in this study. All the queens received a single application of 150 IU Equine Chorionic Gonadotropin (eCG) in the post estrus to induce ovarian activity and 80 to 84 hours later, received a single application of 100 UI Human Chorionic Gonadotropin (hCG) to induce ovulation. After hCG application, only the donor queens were naturally mated. The receptor queens received extra stimulus for induction of ovulation through manipulation of an intravaginal swab. Five to six days after hCG application, the donor queens were subjected to a laparotomy for embryo collection that was performed by trans-horn uterine washing. On average, six embryos were surgically inovulated. They were classified as type I and III compact morula and blastocysts in four receptor queens. Three animals presented pregnancy confirmed by ultrasound at day 36 and two of these animals gave birth to litters of two and four offsprings, respectively, at 66 and 63 days after induction of ovulation. Except for one still birth, all the offspring developed normally. |
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Exogenous induction of ovarian activity and ovulation and transfer of fresh embryos of domestic cat (Felis catus)ovarian activity inductionovulationembryo transferdomestic catThe objective of the present study was the exogenous stimulation of ovarian activity and definition of embryo collection, and transfer protocols, in the domestic cat for potential application in non-domestic endangered species. Sixteen adult queens and two adult male reproducers kept in the experimental cat house at the Morphology sector at the Veterinary Department (DVT), UFV, were used in this study. All the queens received a single application of 150 IU Equine Chorionic Gonadotropin (eCG) in the post estrus to induce ovarian activity and 80 to 84 hours later, received a single application of 100 UI Human Chorionic Gonadotropin (hCG) to induce ovulation. After hCG application, only the donor queens were naturally mated. The receptor queens received extra stimulus for induction of ovulation through manipulation of an intravaginal swab. Five to six days after hCG application, the donor queens were subjected to a laparotomy for embryo collection that was performed by trans-horn uterine washing. On average, six embryos were surgically inovulated. They were classified as type I and III compact morula and blastocysts in four receptor queens. Three animals presented pregnancy confirmed by ultrasound at day 36 and two of these animals gave birth to litters of two and four offsprings, respectively, at 66 and 63 days after induction of ovulation. Except for one still birth, all the offspring developed normally.Universidade Federal de Viçosa2012-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0034-737X2012000400010Revista Ceres v.59 n.4 2012reponame:Revista Ceresinstname:Universidade Federal de Viçosa (UFV)instacron:UFV10.1590/S0034-737X2012000400010info:eu-repo/semantics/openAccessSantana,Marcelo LopesPaula,Tarcízio Antônio Rego deCosta,Eduardo Paulino daCosta,Deiler Sampaioeng2012-09-26T00:00:00ZRevista |
dc.title.none.fl_str_mv |
Exogenous induction of ovarian activity and ovulation and transfer of fresh embryos of domestic cat (Felis catus) |
title |
Exogenous induction of ovarian activity and ovulation and transfer of fresh embryos of domestic cat (Felis catus) |
spellingShingle |
Exogenous induction of ovarian activity and ovulation and transfer of fresh embryos of domestic cat (Felis catus) Santana,Marcelo Lopes ovarian activity induction ovulation embryo transfer domestic cat |
title_short |
Exogenous induction of ovarian activity and ovulation and transfer of fresh embryos of domestic cat (Felis catus) |
title_full |
Exogenous induction of ovarian activity and ovulation and transfer of fresh embryos of domestic cat (Felis catus) |
title_fullStr |
Exogenous induction of ovarian activity and ovulation and transfer of fresh embryos of domestic cat (Felis catus) |
title_full_unstemmed |
Exogenous induction of ovarian activity and ovulation and transfer of fresh embryos of domestic cat (Felis catus) |
title_sort |
Exogenous induction of ovarian activity and ovulation and transfer of fresh embryos of domestic cat (Felis catus) |
author |
Santana,Marcelo Lopes |
author_facet |
Santana,Marcelo Lopes Paula,Tarcízio Antônio Rego de Costa,Eduardo Paulino da Costa,Deiler Sampaio |
author_role |
author |
author2 |
Paula,Tarcízio Antônio Rego de Costa,Eduardo Paulino da Costa,Deiler Sampaio |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Santana,Marcelo Lopes Paula,Tarcízio Antônio Rego de Costa,Eduardo Paulino da Costa,Deiler Sampaio |
dc.subject.por.fl_str_mv |
ovarian activity induction ovulation embryo transfer domestic cat |
topic |
ovarian activity induction ovulation embryo transfer domestic cat |
dc.description.none.fl_txt_mv |
The objective of the present study was the exogenous stimulation of ovarian activity and definition of embryo collection, and transfer protocols, in the domestic cat for potential application in non-domestic endangered species. Sixteen adult queens and two adult male reproducers kept in the experimental cat house at the Morphology sector at the Veterinary Department (DVT), UFV, were used in this study. All the queens received a single application of 150 IU Equine Chorionic Gonadotropin (eCG) in the post estrus to induce ovarian activity and 80 to 84 hours later, received a single application of 100 UI Human Chorionic Gonadotropin (hCG) to induce ovulation. After hCG application, only the donor queens were naturally mated. The receptor queens received extra stimulus for induction of ovulation through manipulation of an intravaginal swab. Five to six days after hCG application, the donor queens were subjected to a laparotomy for embryo collection that was performed by trans-horn uterine washing. On average, six embryos were surgically inovulated. They were classified as type I and III compact morula and blastocysts in four receptor queens. Three animals presented pregnancy confirmed by ultrasound at day 36 and two of these animals gave birth to litters of two and four offsprings, respectively, at 66 and 63 days after induction of ovulation. Except for one still birth, all the offspring developed normally. |
description |
The objective of the present study was the exogenous stimulation of ovarian activity and definition of embryo collection, and transfer protocols, in the domestic cat for potential application in non-domestic endangered species. Sixteen adult queens and two adult male reproducers kept in the experimental cat house at the Morphology sector at the Veterinary Department (DVT), UFV, were used in this study. All the queens received a single application of 150 IU Equine Chorionic Gonadotropin (eCG) in the post estrus to induce ovarian activity and 80 to 84 hours later, received a single application of 100 UI Human Chorionic Gonadotropin (hCG) to induce ovulation. After hCG application, only the donor queens were naturally mated. The receptor queens received extra stimulus for induction of ovulation through manipulation of an intravaginal swab. Five to six days after hCG application, the donor queens were subjected to a laparotomy for embryo collection that was performed by trans-horn uterine washing. On average, six embryos were surgically inovulated. They were classified as type I and III compact morula and blastocysts in four receptor queens. Three animals presented pregnancy confirmed by ultrasound at day 36 and two of these animals gave birth to litters of two and four offsprings, respectively, at 66 and 63 days after induction of ovulation. Except for one still birth, all the offspring developed normally. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0034-737X2012000400010 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0034-737X2012000400010 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0034-737X2012000400010 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Viçosa |
publisher.none.fl_str_mv |
Universidade Federal de Viçosa |
dc.source.none.fl_str_mv |
Revista Ceres v.59 n.4 2012 reponame:Revista Ceres instname:Universidade Federal de Viçosa (UFV) instacron:UFV |
instname_str |
Universidade Federal de Viçosa (UFV) |
instacron_str |
UFV |
institution |
UFV |
reponame_str |
Revista Ceres |
collection |
Revista Ceres |
repository.name.fl_str_mv |
|
repository.mail.fl_str_mv |
|
_version_ |
1728006780335685632 |