Exogenous induction of ovarian activity and ovulation and transfer of fresh embryos of domestic cat (Felis catus)

Detalhes bibliográficos
Autor(a) principal: Santana,Marcelo Lopes
Data de Publicação: 2012
Outros Autores: Paula,Tarcízio Antônio Rego de, Costa,Eduardo Paulino da, Costa,Deiler Sampaio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Ceres
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0034-737X2012000400010
Resumo: The objective of the present study was the exogenous stimulation of ovarian activity and definition of embryo collection, and transfer protocols, in the domestic cat for potential application in non-domestic endangered species. Sixteen adult queens and two adult male reproducers kept in the experimental cat house at the Morphology sector at the Veterinary Department (DVT), UFV, were used in this study. All the queens received a single application of 150 IU Equine Chorionic Gonadotropin (eCG) in the post estrus to induce ovarian activity and 80 to 84 hours later, received a single application of 100 UI Human Chorionic Gonadotropin (hCG) to induce ovulation. After hCG application, only the donor queens were naturally mated. The receptor queens received extra stimulus for induction of ovulation through manipulation of an intravaginal swab. Five to six days after hCG application, the donor queens were subjected to a laparotomy for embryo collection that was performed by trans-horn uterine washing. On average, six embryos were surgically inovulated. They were classified as type I and III compact morula and blastocysts in four receptor queens. Three animals presented pregnancy confirmed by ultrasound at day 36 and two of these animals gave birth to litters of two and four offsprings, respectively, at 66 and 63 days after induction of ovulation. Except for one still birth, all the offspring developed normally.
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spelling Exogenous induction of ovarian activity and ovulation and transfer of fresh embryos of domestic cat (Felis catus)ovarian activity inductionovulationembryo transferdomestic catThe objective of the present study was the exogenous stimulation of ovarian activity and definition of embryo collection, and transfer protocols, in the domestic cat for potential application in non-domestic endangered species. Sixteen adult queens and two adult male reproducers kept in the experimental cat house at the Morphology sector at the Veterinary Department (DVT), UFV, were used in this study. All the queens received a single application of 150 IU Equine Chorionic Gonadotropin (eCG) in the post estrus to induce ovarian activity and 80 to 84 hours later, received a single application of 100 UI Human Chorionic Gonadotropin (hCG) to induce ovulation. After hCG application, only the donor queens were naturally mated. The receptor queens received extra stimulus for induction of ovulation through manipulation of an intravaginal swab. Five to six days after hCG application, the donor queens were subjected to a laparotomy for embryo collection that was performed by trans-horn uterine washing. On average, six embryos were surgically inovulated. They were classified as type I and III compact morula and blastocysts in four receptor queens. Three animals presented pregnancy confirmed by ultrasound at day 36 and two of these animals gave birth to litters of two and four offsprings, respectively, at 66 and 63 days after induction of ovulation. Except for one still birth, all the offspring developed normally.Universidade Federal de Viçosa2012-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0034-737X2012000400010Revista Ceres v.59 n.4 2012reponame:Revista Ceresinstname:Universidade Federal de Viçosa (UFV)instacron:UFV10.1590/S0034-737X2012000400010info:eu-repo/semantics/openAccessSantana,Marcelo LopesPaula,Tarcízio Antônio Rego deCosta,Eduardo Paulino daCosta,Deiler Sampaioeng2012-09-26T00:00:00ZRevista
dc.title.none.fl_str_mv Exogenous induction of ovarian activity and ovulation and transfer of fresh embryos of domestic cat (Felis catus)
title Exogenous induction of ovarian activity and ovulation and transfer of fresh embryos of domestic cat (Felis catus)
spellingShingle Exogenous induction of ovarian activity and ovulation and transfer of fresh embryos of domestic cat (Felis catus)
Santana,Marcelo Lopes
ovarian activity induction
ovulation
embryo transfer
domestic cat
title_short Exogenous induction of ovarian activity and ovulation and transfer of fresh embryos of domestic cat (Felis catus)
title_full Exogenous induction of ovarian activity and ovulation and transfer of fresh embryos of domestic cat (Felis catus)
title_fullStr Exogenous induction of ovarian activity and ovulation and transfer of fresh embryos of domestic cat (Felis catus)
title_full_unstemmed Exogenous induction of ovarian activity and ovulation and transfer of fresh embryos of domestic cat (Felis catus)
title_sort Exogenous induction of ovarian activity and ovulation and transfer of fresh embryos of domestic cat (Felis catus)
author Santana,Marcelo Lopes
author_facet Santana,Marcelo Lopes
Paula,Tarcízio Antônio Rego de
Costa,Eduardo Paulino da
Costa,Deiler Sampaio
author_role author
author2 Paula,Tarcízio Antônio Rego de
Costa,Eduardo Paulino da
Costa,Deiler Sampaio
author2_role author
author
author
dc.contributor.author.fl_str_mv Santana,Marcelo Lopes
Paula,Tarcízio Antônio Rego de
Costa,Eduardo Paulino da
Costa,Deiler Sampaio
dc.subject.por.fl_str_mv ovarian activity induction
ovulation
embryo transfer
domestic cat
topic ovarian activity induction
ovulation
embryo transfer
domestic cat
dc.description.none.fl_txt_mv The objective of the present study was the exogenous stimulation of ovarian activity and definition of embryo collection, and transfer protocols, in the domestic cat for potential application in non-domestic endangered species. Sixteen adult queens and two adult male reproducers kept in the experimental cat house at the Morphology sector at the Veterinary Department (DVT), UFV, were used in this study. All the queens received a single application of 150 IU Equine Chorionic Gonadotropin (eCG) in the post estrus to induce ovarian activity and 80 to 84 hours later, received a single application of 100 UI Human Chorionic Gonadotropin (hCG) to induce ovulation. After hCG application, only the donor queens were naturally mated. The receptor queens received extra stimulus for induction of ovulation through manipulation of an intravaginal swab. Five to six days after hCG application, the donor queens were subjected to a laparotomy for embryo collection that was performed by trans-horn uterine washing. On average, six embryos were surgically inovulated. They were classified as type I and III compact morula and blastocysts in four receptor queens. Three animals presented pregnancy confirmed by ultrasound at day 36 and two of these animals gave birth to litters of two and four offsprings, respectively, at 66 and 63 days after induction of ovulation. Except for one still birth, all the offspring developed normally.
description The objective of the present study was the exogenous stimulation of ovarian activity and definition of embryo collection, and transfer protocols, in the domestic cat for potential application in non-domestic endangered species. Sixteen adult queens and two adult male reproducers kept in the experimental cat house at the Morphology sector at the Veterinary Department (DVT), UFV, were used in this study. All the queens received a single application of 150 IU Equine Chorionic Gonadotropin (eCG) in the post estrus to induce ovarian activity and 80 to 84 hours later, received a single application of 100 UI Human Chorionic Gonadotropin (hCG) to induce ovulation. After hCG application, only the donor queens were naturally mated. The receptor queens received extra stimulus for induction of ovulation through manipulation of an intravaginal swab. Five to six days after hCG application, the donor queens were subjected to a laparotomy for embryo collection that was performed by trans-horn uterine washing. On average, six embryos were surgically inovulated. They were classified as type I and III compact morula and blastocysts in four receptor queens. Three animals presented pregnancy confirmed by ultrasound at day 36 and two of these animals gave birth to litters of two and four offsprings, respectively, at 66 and 63 days after induction of ovulation. Except for one still birth, all the offspring developed normally.
publishDate 2012
dc.date.none.fl_str_mv 2012-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0034-737X2012000400010
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0034-737X2012000400010
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0034-737X2012000400010
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Viçosa
publisher.none.fl_str_mv Universidade Federal de Viçosa
dc.source.none.fl_str_mv Revista Ceres v.59 n.4 2012
reponame:Revista Ceres
instname:Universidade Federal de Viçosa (UFV)
instacron:UFV
instname_str Universidade Federal de Viçosa (UFV)
instacron_str UFV
institution UFV
reponame_str Revista Ceres
collection Revista Ceres
repository.name.fl_str_mv
repository.mail.fl_str_mv
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