Characterization and activity of endophytic bacteria from ‘Prata Anã’ banana crop (Musa sp., AAB)

Detalhes bibliográficos
Autor(a) principal: Pereira,Débora Francine Gomes Silva
Data de Publicação: 2018
Outros Autores: Nietsche,Silvia, Xavier,Adelica Aparecida, Souza,Suzane Ariádina de, Costa,Márcia Regina, Duarte,Anunciene Barbosa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Ceres
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0034-737X2018000500381
Resumo: ABSTRACT The objective of this study was to characterize banana tree endophytic bacteria at genus and species level and to determine the metabolic reactions associated with the nitrogen transformations. The identification at genus and species levels was performed using the partial sequencing of the rDNA 16S region. The assimbyotic nitrogen fixation, the reduction of nitrate and the production of urease were in vitro evaluated. The DNA of the bacterial isolates was also amplified to verify the presence of the nifH, nirK and nirS regions. Biochemical tests were performed in a complete randomized design; the treatments consisted of 39 bacterial isolates with three replications. Sequence analysis enabled the identification of four genera: Bacillus, Rhizobium, Klebsiella and Enterobacter. The Bacillus genus occurred more frequently, nine species were identified. By evaluating the results of biochemical tests, it was observed that three isolates showed multiple abilities: growth in NFb medium, nitrate reduction and production of urease. The isolates belong to the genus Bacillus and of the species subtilis, thuringienses and amyloliquefaciens. Approximately 12.5% of the isolates amplified the region corresponding to the nifH gene, 7.5% amplified gene nirK and 3.9% amplified the nirS gene. Endophytic bacteria evaluated in the present study showed in vitro activity for urease, nitrate reductase enzymes, however, relevant nitrogenase activity was not observed.
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spelling Characterization and activity of endophytic bacteria from ‘Prata Anã’ banana crop (Musa sp., AAB)BacillusdiversitynifHnitrate reductaseureaseABSTRACT The objective of this study was to characterize banana tree endophytic bacteria at genus and species level and to determine the metabolic reactions associated with the nitrogen transformations. The identification at genus and species levels was performed using the partial sequencing of the rDNA 16S region. The assimbyotic nitrogen fixation, the reduction of nitrate and the production of urease were in vitro evaluated. The DNA of the bacterial isolates was also amplified to verify the presence of the nifH, nirK and nirS regions. Biochemical tests were performed in a complete randomized design; the treatments consisted of 39 bacterial isolates with three replications. Sequence analysis enabled the identification of four genera: Bacillus, Rhizobium, Klebsiella and Enterobacter. The Bacillus genus occurred more frequently, nine species were identified. By evaluating the results of biochemical tests, it was observed that three isolates showed multiple abilities: growth in NFb medium, nitrate reduction and production of urease. The isolates belong to the genus Bacillus and of the species subtilis, thuringienses and amyloliquefaciens. Approximately 12.5% of the isolates amplified the region corresponding to the nifH gene, 7.5% amplified gene nirK and 3.9% amplified the nirS gene. Endophytic bacteria evaluated in the present study showed in vitro activity for urease, nitrate reductase enzymes, however, relevant nitrogenase activity was not observed.Universidade Federal de Viçosa2018-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0034-737X2018000500381Revista Ceres v.65 n.5 2018reponame:Revista Ceresinstname:Universidade Federal de Viçosa (UFV)instacron:UFV10.1590/0034-737x201865050001info:eu-repo/semantics/openAccessPereira,Débora Francine Gomes SilvaNietsche,SilviaXavier,Adelica AparecidaSouza,Suzane Ariádina deCosta,Márcia ReginaDuarte,Anunciene Barbosaeng2018-10-26T00:00:00ZRevista
dc.title.none.fl_str_mv Characterization and activity of endophytic bacteria from ‘Prata Anã’ banana crop (Musa sp., AAB)
title Characterization and activity of endophytic bacteria from ‘Prata Anã’ banana crop (Musa sp., AAB)
spellingShingle Characterization and activity of endophytic bacteria from ‘Prata Anã’ banana crop (Musa sp., AAB)
Pereira,Débora Francine Gomes Silva
Bacillus
diversity
nifH
nitrate reductase
urease
title_short Characterization and activity of endophytic bacteria from ‘Prata Anã’ banana crop (Musa sp., AAB)
title_full Characterization and activity of endophytic bacteria from ‘Prata Anã’ banana crop (Musa sp., AAB)
title_fullStr Characterization and activity of endophytic bacteria from ‘Prata Anã’ banana crop (Musa sp., AAB)
title_full_unstemmed Characterization and activity of endophytic bacteria from ‘Prata Anã’ banana crop (Musa sp., AAB)
title_sort Characterization and activity of endophytic bacteria from ‘Prata Anã’ banana crop (Musa sp., AAB)
author Pereira,Débora Francine Gomes Silva
author_facet Pereira,Débora Francine Gomes Silva
Nietsche,Silvia
Xavier,Adelica Aparecida
Souza,Suzane Ariádina de
Costa,Márcia Regina
Duarte,Anunciene Barbosa
author_role author
author2 Nietsche,Silvia
Xavier,Adelica Aparecida
Souza,Suzane Ariádina de
Costa,Márcia Regina
Duarte,Anunciene Barbosa
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Pereira,Débora Francine Gomes Silva
Nietsche,Silvia
Xavier,Adelica Aparecida
Souza,Suzane Ariádina de
Costa,Márcia Regina
Duarte,Anunciene Barbosa
dc.subject.por.fl_str_mv Bacillus
diversity
nifH
nitrate reductase
urease
topic Bacillus
diversity
nifH
nitrate reductase
urease
dc.description.none.fl_txt_mv ABSTRACT The objective of this study was to characterize banana tree endophytic bacteria at genus and species level and to determine the metabolic reactions associated with the nitrogen transformations. The identification at genus and species levels was performed using the partial sequencing of the rDNA 16S region. The assimbyotic nitrogen fixation, the reduction of nitrate and the production of urease were in vitro evaluated. The DNA of the bacterial isolates was also amplified to verify the presence of the nifH, nirK and nirS regions. Biochemical tests were performed in a complete randomized design; the treatments consisted of 39 bacterial isolates with three replications. Sequence analysis enabled the identification of four genera: Bacillus, Rhizobium, Klebsiella and Enterobacter. The Bacillus genus occurred more frequently, nine species were identified. By evaluating the results of biochemical tests, it was observed that three isolates showed multiple abilities: growth in NFb medium, nitrate reduction and production of urease. The isolates belong to the genus Bacillus and of the species subtilis, thuringienses and amyloliquefaciens. Approximately 12.5% of the isolates amplified the region corresponding to the nifH gene, 7.5% amplified gene nirK and 3.9% amplified the nirS gene. Endophytic bacteria evaluated in the present study showed in vitro activity for urease, nitrate reductase enzymes, however, relevant nitrogenase activity was not observed.
description ABSTRACT The objective of this study was to characterize banana tree endophytic bacteria at genus and species level and to determine the metabolic reactions associated with the nitrogen transformations. The identification at genus and species levels was performed using the partial sequencing of the rDNA 16S region. The assimbyotic nitrogen fixation, the reduction of nitrate and the production of urease were in vitro evaluated. The DNA of the bacterial isolates was also amplified to verify the presence of the nifH, nirK and nirS regions. Biochemical tests were performed in a complete randomized design; the treatments consisted of 39 bacterial isolates with three replications. Sequence analysis enabled the identification of four genera: Bacillus, Rhizobium, Klebsiella and Enterobacter. The Bacillus genus occurred more frequently, nine species were identified. By evaluating the results of biochemical tests, it was observed that three isolates showed multiple abilities: growth in NFb medium, nitrate reduction and production of urease. The isolates belong to the genus Bacillus and of the species subtilis, thuringienses and amyloliquefaciens. Approximately 12.5% of the isolates amplified the region corresponding to the nifH gene, 7.5% amplified gene nirK and 3.9% amplified the nirS gene. Endophytic bacteria evaluated in the present study showed in vitro activity for urease, nitrate reductase enzymes, however, relevant nitrogenase activity was not observed.
publishDate 2018
dc.date.none.fl_str_mv 2018-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0034-737X2018000500381
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0034-737X2018000500381
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0034-737x201865050001
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Viçosa
publisher.none.fl_str_mv Universidade Federal de Viçosa
dc.source.none.fl_str_mv Revista Ceres v.65 n.5 2018
reponame:Revista Ceres
instname:Universidade Federal de Viçosa (UFV)
instacron:UFV
instname_str Universidade Federal de Viçosa (UFV)
instacron_str UFV
institution UFV
reponame_str Revista Ceres
collection Revista Ceres
repository.name.fl_str_mv
repository.mail.fl_str_mv
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