Exogenous gibberellin and cytokinin in a novel system for in vitro germination and development of African iris (Dietes bicolor)

Detalhes bibliográficos
Autor(a) principal: Silva,Lázara Aline Simões
Data de Publicação: 2020
Outros Autores: Costa,Andrey de Oliveira, Batista,Diego Silva, Silva,Maurecilne Lemes da, Costa Netto,Antônio Paulino da, Rocha,Diego Ismael
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Ceres
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0034-737X2020000500402
Resumo: ABSTRACT The availability of reliable and reproducible micropropagation systems is a decisive step to successfully propagate plant species with slow growth and low germination rate. The effect was investigated of gibberellin (GA) and cytokinin (CK) in the in vitro germination and development of Dietes bicolor (Steud.) Sweet ex Klatt. Seeds were inoculated in Water + Agar; in Murashige e Skoog (MS) medium without plant growth regulators (PGRs) and in MS medium supplemented with 1 or 2 mg L-1 gibberellic acid or 6-benzylaminopurine. The germination, growth, and architecture of the plants presented significant alterations according to the treatments. Germination percentage increased at 2 mg L-1 GA. Plants grown on the GA-supplemented medium also showed larger leaves. The presence of CK in the culture medium induced the activation of axillary shoot buds and reduced the root length. CK and GA had antagonistic effects on the thickness of the leaf tissues. The activation of multiple shoots in plants cultured with CK in the medium allowed the in vitro propagation of approximately three plants per germinated seed. The results obtained in the present study bring an unprecedented description of a germination system for a higher yield in D. bicolor micropropagation.
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spelling Exogenous gibberellin and cytokinin in a novel system for in vitro germination and development of African iris (Dietes bicolor)butterfly irisiridaceaein vitro germinationplant growth regulatorsmicropropagationshoot proliferationABSTRACT The availability of reliable and reproducible micropropagation systems is a decisive step to successfully propagate plant species with slow growth and low germination rate. The effect was investigated of gibberellin (GA) and cytokinin (CK) in the in vitro germination and development of Dietes bicolor (Steud.) Sweet ex Klatt. Seeds were inoculated in Water + Agar; in Murashige e Skoog (MS) medium without plant growth regulators (PGRs) and in MS medium supplemented with 1 or 2 mg L-1 gibberellic acid or 6-benzylaminopurine. The germination, growth, and architecture of the plants presented significant alterations according to the treatments. Germination percentage increased at 2 mg L-1 GA. Plants grown on the GA-supplemented medium also showed larger leaves. The presence of CK in the culture medium induced the activation of axillary shoot buds and reduced the root length. CK and GA had antagonistic effects on the thickness of the leaf tissues. The activation of multiple shoots in plants cultured with CK in the medium allowed the in vitro propagation of approximately three plants per germinated seed. The results obtained in the present study bring an unprecedented description of a germination system for a higher yield in D. bicolor micropropagation.Universidade Federal de Viçosa2020-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0034-737X2020000500402Revista Ceres v.67 n.5 2020reponame:Revista Ceresinstname:Universidade Federal de Viçosa (UFV)instacron:UFV10.1590/0034-737x202067050008info:eu-repo/semantics/openAccessSilva,Lázara Aline SimõesCosta,Andrey de OliveiraBatista,Diego SilvaSilva,Maurecilne Lemes daCosta Netto,Antônio Paulino daRocha,Diego Ismaeleng2020-09-30T00:00:00ZRevista
dc.title.none.fl_str_mv Exogenous gibberellin and cytokinin in a novel system for in vitro germination and development of African iris (Dietes bicolor)
title Exogenous gibberellin and cytokinin in a novel system for in vitro germination and development of African iris (Dietes bicolor)
spellingShingle Exogenous gibberellin and cytokinin in a novel system for in vitro germination and development of African iris (Dietes bicolor)
Silva,Lázara Aline Simões
butterfly iris
iridaceae
in vitro germination
plant growth regulators
micropropagation
shoot proliferation
title_short Exogenous gibberellin and cytokinin in a novel system for in vitro germination and development of African iris (Dietes bicolor)
title_full Exogenous gibberellin and cytokinin in a novel system for in vitro germination and development of African iris (Dietes bicolor)
title_fullStr Exogenous gibberellin and cytokinin in a novel system for in vitro germination and development of African iris (Dietes bicolor)
title_full_unstemmed Exogenous gibberellin and cytokinin in a novel system for in vitro germination and development of African iris (Dietes bicolor)
title_sort Exogenous gibberellin and cytokinin in a novel system for in vitro germination and development of African iris (Dietes bicolor)
author Silva,Lázara Aline Simões
author_facet Silva,Lázara Aline Simões
Costa,Andrey de Oliveira
Batista,Diego Silva
Silva,Maurecilne Lemes da
Costa Netto,Antônio Paulino da
Rocha,Diego Ismael
author_role author
author2 Costa,Andrey de Oliveira
Batista,Diego Silva
Silva,Maurecilne Lemes da
Costa Netto,Antônio Paulino da
Rocha,Diego Ismael
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Silva,Lázara Aline Simões
Costa,Andrey de Oliveira
Batista,Diego Silva
Silva,Maurecilne Lemes da
Costa Netto,Antônio Paulino da
Rocha,Diego Ismael
dc.subject.por.fl_str_mv butterfly iris
iridaceae
in vitro germination
plant growth regulators
micropropagation
shoot proliferation
topic butterfly iris
iridaceae
in vitro germination
plant growth regulators
micropropagation
shoot proliferation
dc.description.none.fl_txt_mv ABSTRACT The availability of reliable and reproducible micropropagation systems is a decisive step to successfully propagate plant species with slow growth and low germination rate. The effect was investigated of gibberellin (GA) and cytokinin (CK) in the in vitro germination and development of Dietes bicolor (Steud.) Sweet ex Klatt. Seeds were inoculated in Water + Agar; in Murashige e Skoog (MS) medium without plant growth regulators (PGRs) and in MS medium supplemented with 1 or 2 mg L-1 gibberellic acid or 6-benzylaminopurine. The germination, growth, and architecture of the plants presented significant alterations according to the treatments. Germination percentage increased at 2 mg L-1 GA. Plants grown on the GA-supplemented medium also showed larger leaves. The presence of CK in the culture medium induced the activation of axillary shoot buds and reduced the root length. CK and GA had antagonistic effects on the thickness of the leaf tissues. The activation of multiple shoots in plants cultured with CK in the medium allowed the in vitro propagation of approximately three plants per germinated seed. The results obtained in the present study bring an unprecedented description of a germination system for a higher yield in D. bicolor micropropagation.
description ABSTRACT The availability of reliable and reproducible micropropagation systems is a decisive step to successfully propagate plant species with slow growth and low germination rate. The effect was investigated of gibberellin (GA) and cytokinin (CK) in the in vitro germination and development of Dietes bicolor (Steud.) Sweet ex Klatt. Seeds were inoculated in Water + Agar; in Murashige e Skoog (MS) medium without plant growth regulators (PGRs) and in MS medium supplemented with 1 or 2 mg L-1 gibberellic acid or 6-benzylaminopurine. The germination, growth, and architecture of the plants presented significant alterations according to the treatments. Germination percentage increased at 2 mg L-1 GA. Plants grown on the GA-supplemented medium also showed larger leaves. The presence of CK in the culture medium induced the activation of axillary shoot buds and reduced the root length. CK and GA had antagonistic effects on the thickness of the leaf tissues. The activation of multiple shoots in plants cultured with CK in the medium allowed the in vitro propagation of approximately three plants per germinated seed. The results obtained in the present study bring an unprecedented description of a germination system for a higher yield in D. bicolor micropropagation.
publishDate 2020
dc.date.none.fl_str_mv 2020-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0034-737X2020000500402
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0034-737X2020000500402
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0034-737x202067050008
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Viçosa
publisher.none.fl_str_mv Universidade Federal de Viçosa
dc.source.none.fl_str_mv Revista Ceres v.67 n.5 2020
reponame:Revista Ceres
instname:Universidade Federal de Viçosa (UFV)
instacron:UFV
instname_str Universidade Federal de Viçosa (UFV)
instacron_str UFV
institution UFV
reponame_str Revista Ceres
collection Revista Ceres
repository.name.fl_str_mv
repository.mail.fl_str_mv
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