Microbiological viability of bovine amniotic membrane stored in glycerin 99% at room temperature for 48 months

Detalhes bibliográficos
Autor(a) principal: Pontes,Kelly Cristine de Sousa
Data de Publicação: 2017
Outros Autores: Ramalho,Camila Almeida, Borges,Andrea Pacheco Batista, Sepúlveda,Rodrigo Viana, Eleotério,Renato Barros
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Ceres
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0034-737X2017000100055
Resumo: ABSTRACT The medium for storing biological tissues is of great importance for their optimal use in surgery. Glycerin has been proven efficient for storing diverse tissues for prolonged time, but the preservation of the bovine amniotic membrane in glycerin 99% at room temperature has never been evaluated to be used safely in surgical procedures. This study evaluated the preservation of 80 bovine amniotic membrane samples stored in glycerin 99% at room temperature. The samples were randomly divided evenly into four groups. Samples were microbiologically tested after 1, 6, 12 and 48 months of storage. The presence of bacteria and fungi in the samples was evaluated by inoculation on blood agar and incubation at 37 ºC for 48 hours and on Sabouraud agar at 25 ºC for 5 to 10 days. No fungal or bacterial growth was detected in any of the samples. It was concluded that glycerin is an efficient medium, regarding microbiology, for preserving pre-prepared bovine amniotic membrane, keeping the tissue free of microorganisms that grow in the media up to 48 months at room temperature.
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spelling Microbiological viability of bovine amniotic membrane stored in glycerin 99% at room temperature for 48 monthsbiological membranespreservation mediumsurgeryamnioncaesarean sectionABSTRACT The medium for storing biological tissues is of great importance for their optimal use in surgery. Glycerin has been proven efficient for storing diverse tissues for prolonged time, but the preservation of the bovine amniotic membrane in glycerin 99% at room temperature has never been evaluated to be used safely in surgical procedures. This study evaluated the preservation of 80 bovine amniotic membrane samples stored in glycerin 99% at room temperature. The samples were randomly divided evenly into four groups. Samples were microbiologically tested after 1, 6, 12 and 48 months of storage. The presence of bacteria and fungi in the samples was evaluated by inoculation on blood agar and incubation at 37 ºC for 48 hours and on Sabouraud agar at 25 ºC for 5 to 10 days. No fungal or bacterial growth was detected in any of the samples. It was concluded that glycerin is an efficient medium, regarding microbiology, for preserving pre-prepared bovine amniotic membrane, keeping the tissue free of microorganisms that grow in the media up to 48 months at room temperature.Universidade Federal de Viçosa2017-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0034-737X2017000100055Revista Ceres v.64 n.1 2017reponame:Revista Ceresinstname:Universidade Federal de Viçosa (UFV)instacron:UFV10.1590/0034-737x201764010008info:eu-repo/semantics/openAccessPontes,Kelly Cristine de SousaRamalho,Camila AlmeidaBorges,Andrea Pacheco BatistaSepúlveda,Rodrigo VianaEleotério,Renato Barroseng2017-03-22T00:00:00ZRevista
dc.title.none.fl_str_mv Microbiological viability of bovine amniotic membrane stored in glycerin 99% at room temperature for 48 months
title Microbiological viability of bovine amniotic membrane stored in glycerin 99% at room temperature for 48 months
spellingShingle Microbiological viability of bovine amniotic membrane stored in glycerin 99% at room temperature for 48 months
Pontes,Kelly Cristine de Sousa
biological membranes
preservation medium
surgery
amnion
caesarean section
title_short Microbiological viability of bovine amniotic membrane stored in glycerin 99% at room temperature for 48 months
title_full Microbiological viability of bovine amniotic membrane stored in glycerin 99% at room temperature for 48 months
title_fullStr Microbiological viability of bovine amniotic membrane stored in glycerin 99% at room temperature for 48 months
title_full_unstemmed Microbiological viability of bovine amniotic membrane stored in glycerin 99% at room temperature for 48 months
title_sort Microbiological viability of bovine amniotic membrane stored in glycerin 99% at room temperature for 48 months
author Pontes,Kelly Cristine de Sousa
author_facet Pontes,Kelly Cristine de Sousa
Ramalho,Camila Almeida
Borges,Andrea Pacheco Batista
Sepúlveda,Rodrigo Viana
Eleotério,Renato Barros
author_role author
author2 Ramalho,Camila Almeida
Borges,Andrea Pacheco Batista
Sepúlveda,Rodrigo Viana
Eleotério,Renato Barros
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Pontes,Kelly Cristine de Sousa
Ramalho,Camila Almeida
Borges,Andrea Pacheco Batista
Sepúlveda,Rodrigo Viana
Eleotério,Renato Barros
dc.subject.por.fl_str_mv biological membranes
preservation medium
surgery
amnion
caesarean section
topic biological membranes
preservation medium
surgery
amnion
caesarean section
dc.description.none.fl_txt_mv ABSTRACT The medium for storing biological tissues is of great importance for their optimal use in surgery. Glycerin has been proven efficient for storing diverse tissues for prolonged time, but the preservation of the bovine amniotic membrane in glycerin 99% at room temperature has never been evaluated to be used safely in surgical procedures. This study evaluated the preservation of 80 bovine amniotic membrane samples stored in glycerin 99% at room temperature. The samples were randomly divided evenly into four groups. Samples were microbiologically tested after 1, 6, 12 and 48 months of storage. The presence of bacteria and fungi in the samples was evaluated by inoculation on blood agar and incubation at 37 ºC for 48 hours and on Sabouraud agar at 25 ºC for 5 to 10 days. No fungal or bacterial growth was detected in any of the samples. It was concluded that glycerin is an efficient medium, regarding microbiology, for preserving pre-prepared bovine amniotic membrane, keeping the tissue free of microorganisms that grow in the media up to 48 months at room temperature.
description ABSTRACT The medium for storing biological tissues is of great importance for their optimal use in surgery. Glycerin has been proven efficient for storing diverse tissues for prolonged time, but the preservation of the bovine amniotic membrane in glycerin 99% at room temperature has never been evaluated to be used safely in surgical procedures. This study evaluated the preservation of 80 bovine amniotic membrane samples stored in glycerin 99% at room temperature. The samples were randomly divided evenly into four groups. Samples were microbiologically tested after 1, 6, 12 and 48 months of storage. The presence of bacteria and fungi in the samples was evaluated by inoculation on blood agar and incubation at 37 ºC for 48 hours and on Sabouraud agar at 25 ºC for 5 to 10 days. No fungal or bacterial growth was detected in any of the samples. It was concluded that glycerin is an efficient medium, regarding microbiology, for preserving pre-prepared bovine amniotic membrane, keeping the tissue free of microorganisms that grow in the media up to 48 months at room temperature.
publishDate 2017
dc.date.none.fl_str_mv 2017-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0034-737X2017000100055
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0034-737X2017000100055
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0034-737x201764010008
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Viçosa
publisher.none.fl_str_mv Universidade Federal de Viçosa
dc.source.none.fl_str_mv Revista Ceres v.64 n.1 2017
reponame:Revista Ceres
instname:Universidade Federal de Viçosa (UFV)
instacron:UFV
instname_str Universidade Federal de Viçosa (UFV)
instacron_str UFV
institution UFV
reponame_str Revista Ceres
collection Revista Ceres
repository.name.fl_str_mv
repository.mail.fl_str_mv
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