Identifica??o e atividade enzim?tica de fungos celulol?ticos oriundos de solos sob plantio de Eucalyptus spp.

Detalhes bibliográficos
Autor(a) principal: Docha, Maria Cec?lia Mota
Data de Publicação: 2020
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFVJM
Texto Completo: http://acervo.ufvjm.edu.br/jspui/handle/1/2384
Resumo: O presente trabalho foi realizado com apoio da Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - Brasil (CAPES) - C?digo de Financiamento 001.
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spelling Docha, Maria Cec?lia MotaLaia, Marcelo Luiz deAbrah?o, Christ?v?o PereiraBara?na, Edy Eime PereiraUniversidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM)Laia, Marcelo Luiz de2021-01-05T14:02:11Z2021-01-05T14:02:11Z20202020-03-04DOCHA, Maria Cec?lia Mota. Identifica??o e atividade enzim?tica de fungos celulol?ticos oriundos de solos sob plantio de Eucalyptus spp. 2020. 45 p. Disserta??o (Mestrado em Ci?ncia Florestal) ? Programa de P?s-Gradua??o em Ci?ncia Florestal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina, 2020.http://acervo.ufvjm.edu.br/jspui/handle/1/2384O presente trabalho foi realizado com apoio da Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - Brasil (CAPES) - C?digo de Financiamento 001.Na natureza, micro-organismos, como fungos e bact?rias, naturalmente degradam os constituintes da madeira, tais como hemicelulose, celulose e lignina. Esta atividade ? importante para o meio ambiente, pois realizam a degrada??o da mat?ria org?nica depositada sobre o solo em plantios florestais ou em florestas naturais. Para produ??o de a??cares ferment?veis ou bioenergia, ? utilizado biomassa celul?sica, sendo considerada uma alternativa ben?fica, ao qual necessita da produ??o enzim?tica de micro-organismos. O presente trabalho tem como objetivo, identificar e analisar a atividade enzim?tica de fungos de um banco de micro-organismos celulol?ticos oriundos de solo e serapilheira sob plantio de eucalipto. A identifica??o foi realizada para cinco fungos, por meio de t?cnicas biomoleculares, como: extra??o de DNA, quantifica??o visual em gel de agarose, PCR e sequenciamento de DNA. Foram selecionados dois fungos celulol?ticos, que atenderam os crit?rios para a an?lise de atividade enzim?tica de beta-glicosidase e endoglucanase, submetidos a meio l?quido de cultivo, in vitro, de CMC (carboximetilcelulose) e de p? de madeira de eucalipto. Os cinco fungos identificados s?o ascomicetos, onde 4 apresentaram homologia acima de 99%, sendo pertencentes aos g?neros: Penicillium para IM1-12, Simplicillium para IM32-12, Exophiala para IM7-21, Rhinocladiella para IM1-58, e g?nero n?o definido para IM1-34B, evidenciando a diversidade do banco gen?tico estudado. A produ??o de beta-glicosidase e endoglucanase alcan?aram, respectivamente, valores de 0,109 U.mL-1 e 0,587 U.mL-1 para o isolado IM32-12, e 0,090 U.mL-1 e 0,505 U.mL-1 para o isolado IM1-12. O meio de cultivo com CMC foi mais eficiente para a produ??o enzim?tica. O isolado IM32-12 apresentou maior valor para produ??o enzim?tica tanto de atividade de beta-glicosidase quanto de endoglucanase, nos diferentes meios de cultivo, demonstrando maior potencial enzim?tico em rela??o ao isolado IM1-12. Contudo, recomenda-se novos estudos voltados para a otimiza??o da produ??o enzim?tica desses micro-organismos, com intuito de promover o m?ximo potencial enzim?tico.Submitted by Jos? Henrique Henrique (jose.neves@ufvjm.edu.br) on 2021-01-05T14:01:29Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) maria_cecilia_mota_docha.pdf: 1042221 bytes, checksum: cf2d9c2b70d609d35f4d55410896c97c (MD5)Approved for entry into archive by Jos? Henrique Henrique (jose.neves@ufvjm.edu.br) on 2021-01-05T14:02:11Z (GMT) No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) maria_cecilia_mota_docha.pdf: 1042221 bytes, checksum: cf2d9c2b70d609d35f4d55410896c97c (MD5)Made available in DSpace on 2021-01-05T14:02:11Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) maria_cecilia_mota_docha.pdf: 1042221 bytes, checksum: cf2d9c2b70d609d35f4d55410896c97c (MD5) Previous issue date: 2020Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES)Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Ci?ncia Florestal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2020.In nature, microorganisms, such as fungi and bacteria, naturally degrade the constituents of wood, such as hemicellulose, cellulose and lignin. This activity is important for the environment, since they degrade the organic matter deposited on the soil in forest plantations or in natural forests. For the production of fermentable sugars or bioenergy, cellulosic biomass is used, being considered a beneficial alternative, and qualified by the enzymatic production of microorganisms. The present work aims to identify and analyze the enzymatic activity of fungi from a bank of cellulolytic microorganisms from soil and litter under eucalyptus plantation. The identification was carried out for five fungi, using biomolecular techniques, such as: DNA extraction, visual quantification in agarose gel, PCR and DNA sequencing. Two cellulolytic fungi were selected, which met the criteria for the analysis of enzymatic activity of beta-glycosidase and endoglucanase, submitted to liquid culture medium, in vitro, of CMC (carboxymethyl cellulose) and eucalyptus wood powder. The five fungi identified are ascomycetes, where 4 showed homology above 99%, belonging to the genera: Penicillium for IM1-12, Simplicillium for IM32-12, Exophiala for IM7-21, Rhinocladiella for IM1-58, and gender not defined for IM1-34B, showing the diversity of the genetic bank studied. The production of beta-glucosidase and endoglucanase reached values of 0.109 U.mL-1 and 0.587 U.mL-1, respectively, for IM32-12 alone, and 0.090 U.mL-1 and 0.505 U.mL-1 for the IM1-12 isolated. The CMC culture medium was more efficient for enzymatic production. The isolated IM32-12 showed higher value for enzymatic production of both beta-glucosidase and endoglucanase activity, in the different culture media, showing greater enzymatic potential in relation to the isolated IM1-12. However, new studies aimed at optimizing the enzymatic production of these microorganisms are recommended in order to promote the maximum enzymatic potencial.porUFVJMA concess?o da licen?a deste item refere-se ao ? termo de autoriza??o impresso assinado pelo autor, assim como na licen?a Creative Commons, com as seguintes condi??es: Na qualidade de titular dos direitos de autor da publica??o, autorizo a Universidade Federal dos Vales do Jequitinhonha e Mucuri e o IBICT a disponibilizar por meio de seus reposit?rios, sem ressarcimento dos direitos autorais, de acordo com a Lei n? 9610/98, o texto integral da obra disponibilizada, conforme permiss?es assinaladas, para fins de leitura, impress?o e/ou download, a t?tulo de divulga??o da produ??o cient?fica brasileira, e preserva??o, a partir desta data.info:eu-repo/semantics/openAccessIdentifica??o e atividade enzim?tica de fungos celulol?ticos oriundos de solos sob plantio de Eucalyptus spp.Identification and enzymatic activity of cellulolytic fungi from soils under Eucalyptus spp.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisMicro-organismosCeluloseBioenergiaMicroorganismsCelluloseBioenergyreponame:Repositório Institucional da UFVJMinstname:Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM)instacron:UFVJMTEXTmaria_cecilia_mota_docha.pdf.txtmaria_cecilia_mota_docha.pdf.txtExtracted texttext/plain64487http://acervo.ufvjm.edu.br/jspui/bitstream/1/2384/6/maria_cecilia_mota_docha.pdf.txt00815cf73849564c8b0b63872dd3e002MD56LICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv Identifica??o e atividade enzim?tica de fungos celulol?ticos oriundos de solos sob plantio de Eucalyptus spp.
dc.title.alternative.en.fl_str_mv Identification and enzymatic activity of cellulolytic fungi from soils under Eucalyptus spp.
title Identifica??o e atividade enzim?tica de fungos celulol?ticos oriundos de solos sob plantio de Eucalyptus spp.
spellingShingle Identifica??o e atividade enzim?tica de fungos celulol?ticos oriundos de solos sob plantio de Eucalyptus spp.
Docha, Maria Cec?lia Mota
Micro-organismos
Celulose
Bioenergia
Microorganisms
Cellulose
Bioenergy
title_short Identifica??o e atividade enzim?tica de fungos celulol?ticos oriundos de solos sob plantio de Eucalyptus spp.
title_full Identifica??o e atividade enzim?tica de fungos celulol?ticos oriundos de solos sob plantio de Eucalyptus spp.
title_fullStr Identifica??o e atividade enzim?tica de fungos celulol?ticos oriundos de solos sob plantio de Eucalyptus spp.
title_full_unstemmed Identifica??o e atividade enzim?tica de fungos celulol?ticos oriundos de solos sob plantio de Eucalyptus spp.
title_sort Identifica??o e atividade enzim?tica de fungos celulol?ticos oriundos de solos sob plantio de Eucalyptus spp.
author Docha, Maria Cec?lia Mota
author_facet Docha, Maria Cec?lia Mota
author_role author
dc.contributor.referee.none.fl_str_mv Laia, Marcelo Luiz de
Abrah?o, Christ?v?o Pereira
Bara?na, Edy Eime Pereira
dc.contributor.institution.pt_BR.fl_str_mv Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM)
dc.contributor.author.fl_str_mv Docha, Maria Cec?lia Mota
dc.contributor.advisor1.fl_str_mv Laia, Marcelo Luiz de
contributor_str_mv Laia, Marcelo Luiz de
dc.subject.keyword.pt_BR.fl_str_mv Micro-organismos
Celulose
Bioenergia
topic Micro-organismos
Celulose
Bioenergia
Microorganisms
Cellulose
Bioenergy
dc.subject.keyword.en.fl_str_mv Microorganisms
Cellulose
Bioenergy
description O presente trabalho foi realizado com apoio da Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - Brasil (CAPES) - C?digo de Financiamento 001.
publishDate 2020
dc.date.submitted.none.fl_str_mv 2020-03-04
dc.date.issued.fl_str_mv 2020
dc.date.accessioned.fl_str_mv 2021-01-05T14:02:11Z
dc.date.available.fl_str_mv 2021-01-05T14:02:11Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv DOCHA, Maria Cec?lia Mota. Identifica??o e atividade enzim?tica de fungos celulol?ticos oriundos de solos sob plantio de Eucalyptus spp. 2020. 45 p. Disserta??o (Mestrado em Ci?ncia Florestal) ? Programa de P?s-Gradua??o em Ci?ncia Florestal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina, 2020.
dc.identifier.uri.fl_str_mv http://acervo.ufvjm.edu.br/jspui/handle/1/2384
identifier_str_mv DOCHA, Maria Cec?lia Mota. Identifica??o e atividade enzim?tica de fungos celulol?ticos oriundos de solos sob plantio de Eucalyptus spp. 2020. 45 p. Disserta??o (Mestrado em Ci?ncia Florestal) ? Programa de P?s-Gradua??o em Ci?ncia Florestal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina, 2020.
url http://acervo.ufvjm.edu.br/jspui/handle/1/2384
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv UFVJM
publisher.none.fl_str_mv UFVJM
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFVJM
instname:Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM)
instacron:UFVJM
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instacron_str UFVJM
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reponame_str Repositório Institucional da UFVJM
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