Cell aggregation in monolayer culture: Clues to a universal kinetics
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | https://doi.org/10.1016/j.physa.2018.07.035 http://www.locus.ufv.br/handle/123456789/21528 |
Resumo: | Aggregation of animal cells in culture comprises a series of motility, collision and adhesion processes of basic relevance for tissue engineering, bioseparations, oncology research and in vitro drug testing. In the present paper, we characterize the aggregation kinetics of normal and cancer cells in culture by determining their cluster size distribution functions. Our experiments reveal that the complementary cumulative distributions for eight cell lines from epithelial and mesenchymal origins are described by stretched exponential functions. Also, we find that as time evolves and the cell density increases, a dynamical phase transition occurs from an aggregation regime described by monotonically decreasing cluster size distributions to another one in which these distributions are nonmonotonic, exhibiting local maxima at large cluster sizes. Subtly, the universality of the stretched exponential distributions admits distinct cluster growth processes controlled by motility and adhesion traits of the cells. |
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Santos, A.A.Martins, M.L.Alves, S.G.Silva, P.C.A. da2018-08-30T10:49:26Z2018-08-30T10:49:26Z2018-07-180378-4371https://doi.org/10.1016/j.physa.2018.07.035http://www.locus.ufv.br/handle/123456789/21528Aggregation of animal cells in culture comprises a series of motility, collision and adhesion processes of basic relevance for tissue engineering, bioseparations, oncology research and in vitro drug testing. In the present paper, we characterize the aggregation kinetics of normal and cancer cells in culture by determining their cluster size distribution functions. Our experiments reveal that the complementary cumulative distributions for eight cell lines from epithelial and mesenchymal origins are described by stretched exponential functions. Also, we find that as time evolves and the cell density increases, a dynamical phase transition occurs from an aggregation regime described by monotonically decreasing cluster size distributions to another one in which these distributions are nonmonotonic, exhibiting local maxima at large cluster sizes. Subtly, the universality of the stretched exponential distributions admits distinct cluster growth processes controlled by motility and adhesion traits of the cells.engPhysica A: Statistical Mechanics and its ApplicationsVolume 510, Pages 725-740, July 2018Elsevier B.Vinfo:eu-repo/semantics/openAccessAggregation processesCell cultureCluster size distributionsHybrid agent-based modelingCell aggregation in monolayer culture: Clues to a universal kineticsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdfTexto completoapplication/pdf2553943https://locus.ufv.br//bitstream/123456789/21528/1/artigo.pdf0f2c663c3032cc7d693973c6f63afbb1MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/21528/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILartigo.pdf.jpgartigo.pdf.jpgIM Thumbnailimage/jpeg5093https://locus.ufv.br//bitstream/123456789/21528/3/artigo.pdf.jpgcfdfbad7df627a91ab95e2fbd85f0cbfMD53123456789/215282018-08-30 23:00:35.348oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452018-08-31T02:00:35LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.en.fl_str_mv |
Cell aggregation in monolayer culture: Clues to a universal kinetics |
title |
Cell aggregation in monolayer culture: Clues to a universal kinetics |
spellingShingle |
Cell aggregation in monolayer culture: Clues to a universal kinetics Santos, A.A. Aggregation processes Cell culture Cluster size distributions Hybrid agent-based modeling |
title_short |
Cell aggregation in monolayer culture: Clues to a universal kinetics |
title_full |
Cell aggregation in monolayer culture: Clues to a universal kinetics |
title_fullStr |
Cell aggregation in monolayer culture: Clues to a universal kinetics |
title_full_unstemmed |
Cell aggregation in monolayer culture: Clues to a universal kinetics |
title_sort |
Cell aggregation in monolayer culture: Clues to a universal kinetics |
author |
Santos, A.A. |
author_facet |
Santos, A.A. Martins, M.L. Alves, S.G. Silva, P.C.A. da |
author_role |
author |
author2 |
Martins, M.L. Alves, S.G. Silva, P.C.A. da |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Santos, A.A. Martins, M.L. Alves, S.G. Silva, P.C.A. da |
dc.subject.pt-BR.fl_str_mv |
Aggregation processes Cell culture Cluster size distributions Hybrid agent-based modeling |
topic |
Aggregation processes Cell culture Cluster size distributions Hybrid agent-based modeling |
description |
Aggregation of animal cells in culture comprises a series of motility, collision and adhesion processes of basic relevance for tissue engineering, bioseparations, oncology research and in vitro drug testing. In the present paper, we characterize the aggregation kinetics of normal and cancer cells in culture by determining their cluster size distribution functions. Our experiments reveal that the complementary cumulative distributions for eight cell lines from epithelial and mesenchymal origins are described by stretched exponential functions. Also, we find that as time evolves and the cell density increases, a dynamical phase transition occurs from an aggregation regime described by monotonically decreasing cluster size distributions to another one in which these distributions are nonmonotonic, exhibiting local maxima at large cluster sizes. Subtly, the universality of the stretched exponential distributions admits distinct cluster growth processes controlled by motility and adhesion traits of the cells. |
publishDate |
2018 |
dc.date.accessioned.fl_str_mv |
2018-08-30T10:49:26Z |
dc.date.available.fl_str_mv |
2018-08-30T10:49:26Z |
dc.date.issued.fl_str_mv |
2018-07-18 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.1016/j.physa.2018.07.035 http://www.locus.ufv.br/handle/123456789/21528 |
dc.identifier.issn.none.fl_str_mv |
0378-4371 |
identifier_str_mv |
0378-4371 |
url |
https://doi.org/10.1016/j.physa.2018.07.035 http://www.locus.ufv.br/handle/123456789/21528 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.pt-BR.fl_str_mv |
Volume 510, Pages 725-740, July 2018 |
dc.rights.driver.fl_str_mv |
Elsevier B.V info:eu-repo/semantics/openAccess |
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Elsevier B.V |
eu_rights_str_mv |
openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
Physica A: Statistical Mechanics and its Applications |
publisher.none.fl_str_mv |
Physica A: Statistical Mechanics and its Applications |
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LOCUS Repositório Institucional da UFV |
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