Somatic embryogenesis in Carica papaya as affected by auxins and explants, and morphoanatomical-related aspects
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | http://dx.doi.org/10.1590/0001-3765201820160252 http://www.locus.ufv.br/handle/123456789/18791 |
Resumo: | The aim of this study was to evaluate somatic embryogenesis in juvenile explants of the THB papaya cultivar. Apical shoots and cotyledonary leaves were inoculated in an induction medium composed of different concentrations of 2,4-D (6, 9, 12, 15 and 18 µM) or 4-CPA (19, 22, 25, 28 and 31 µM). The embryogenic calluses were transferred to a maturation medium for 30 days. Histological analysis were done during the induction and scanning electron microscopy after maturing. For both types of auxin, embryogenesis was achieved at higher frequencies with cotyledonary leaves incubated in induction medium than with apical shoots; except for callogenesis. The early-stage embryos (e.g., globular or heart-shape) predominated. Among the auxins, best results were observed in cotyledonary leaves induced with 4-CPA (25 µM). Histological analyses of the cotyledonary leaf-derived calluses confirmed that the somatic embryos (SEs) formed from parenchyma cells, predominantly differentiated via indirect and multicellular origin and infrequently via synchronized embryogenesis. The secondary embryogenesis was observed during induction and maturation phases in papaya THB cultivar. The combination of ABA (0.5 µM) and AC (15 g L^-1) in maturation medium resulted in the highest somatic embryogenesis induction frequency (70 SEs callus^-1) and the lowest percentage of early germination (4%). |
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Cruz, Ana Cláudia F.Cipriano, Jamile L.D.Mancini, Karina C.Schmildt, Edilson R.Lopes, José CarlosOtoni, Wagner C.Alexandre, Rodrigo S.2018-04-18T14:09:46Z2018-04-18T14:09:46Z2018-02-0116782690http://dx.doi.org/10.1590/0001-3765201820160252http://www.locus.ufv.br/handle/123456789/18791The aim of this study was to evaluate somatic embryogenesis in juvenile explants of the THB papaya cultivar. Apical shoots and cotyledonary leaves were inoculated in an induction medium composed of different concentrations of 2,4-D (6, 9, 12, 15 and 18 µM) or 4-CPA (19, 22, 25, 28 and 31 µM). The embryogenic calluses were transferred to a maturation medium for 30 days. Histological analysis were done during the induction and scanning electron microscopy after maturing. For both types of auxin, embryogenesis was achieved at higher frequencies with cotyledonary leaves incubated in induction medium than with apical shoots; except for callogenesis. The early-stage embryos (e.g., globular or heart-shape) predominated. Among the auxins, best results were observed in cotyledonary leaves induced with 4-CPA (25 µM). Histological analyses of the cotyledonary leaf-derived calluses confirmed that the somatic embryos (SEs) formed from parenchyma cells, predominantly differentiated via indirect and multicellular origin and infrequently via synchronized embryogenesis. The secondary embryogenesis was observed during induction and maturation phases in papaya THB cultivar. The combination of ABA (0.5 µM) and AC (15 g L^-1) in maturation medium resulted in the highest somatic embryogenesis induction frequency (70 SEs callus^-1) and the lowest percentage of early germination (4%).engAnais da Academia Brasileira de Ciênciasv. 90, n. 1, p. 385-400, Março 2018Carica papaya L.Juvenile explantsMaturationMicroscopic analysisSynthetic auxinsSomatic embryogenesis in Carica papaya as affected by auxins and explants, and morphoanatomical-related aspectsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdftexto completoapplication/pdf1437342https://locus.ufv.br//bitstream/123456789/18791/1/artigo.pdf19dfaeb607e0697c24177eca831db23bMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/18791/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILartigo.pdf.jpgartigo.pdf.jpgIM Thumbnailimage/jpeg5162https://locus.ufv.br//bitstream/123456789/18791/3/artigo.pdf.jpg270c6107bea67c407a64f2be567f0a14MD53123456789/187912018-04-18 23:00:43.515oai:locus.ufv.br:123456789/18791Tk9URTogUExBQ0UgWU9VUiBPV04gTElDRU5TRSBIRVJFClRoaXMgc2FtcGxlIGxpY2Vuc2UgaXMgcHJvdmlkZWQgZm9yIGluZm9ybWF0aW9uYWwgcHVycG9zZXMgb25seS4KCk5PTi1FWENMVVNJVkUgRElTVFJJQlVUSU9OIExJQ0VOU0UKCkJ5IHNpZ25pbmcgYW5kIHN1Ym1pdHRpbmcgdGhpcyBsaWNlbnNlLCB5b3UgKHRoZSBhdXRob3Iocykgb3IgY29weXJpZ2h0Cm93bmVyKSBncmFudHMgdG8gRFNwYWNlIFVuaXZlcnNpdHkgKERTVSkgdGhlIG5vbi1leGNsdXNpdmUgcmlnaHQgdG8gcmVwcm9kdWNlLAp0cmFuc2xhdGUgKGFzIGRlZmluZWQgYmVsb3cpLCBhbmQvb3IgZGlzdHJpYnV0ZSB5b3VyIHN1Ym1pc3Npb24gKGluY2x1ZGluZwp0aGUgYWJzdHJhY3QpIHdvcmxkd2lkZSBpbiBwcmludCBhbmQgZWxlY3Ryb25pYyBmb3JtYXQgYW5kIGluIGFueSBtZWRpdW0sCmluY2x1ZGluZyBidXQgbm90IGxpbWl0ZWQgdG8gYXVkaW8gb3IgdmlkZW8uCgpZb3UgYWdyZWUgdGhhdCBEU1UgbWF5LCB3aXRob3V0IGNoYW5naW5nIHRoZSBjb250ZW50LCB0cmFuc2xhdGUgdGhlCnN1Ym1pc3Npb24gdG8gYW55IG1lZGl1bSBvciBmb3JtYXQgZm9yIHRoZSBwdXJwb3NlIG9mIHByZXNlcnZhdGlvbi4KCllvdSBhbHNvIGFncmVlIHRoYXQgRFNVIG1heSBrZWVwIG1vcmUgdGhhbiBvbmUgY29weSBvZiB0aGlzIHN1Ym1pc3Npb24gZm9yCnB1cnBvc2VzIG9mIHNlY3VyaXR5LCBiYWNrLXVwIGFuZCBwcmVzZXJ2YXRpb24uCgpZb3UgcmVwcmVzZW50IHRoYXQgdGhlIHN1Ym1pc3Npb24gaXMgeW91ciBvcmlnaW5hbCB3b3JrLCBhbmQgdGhhdCB5b3UgaGF2ZQp0aGUgcmlnaHQgdG8gZ3JhbnQgdGhlIHJpZ2h0cyBjb250YWluZWQgaW4gdGhpcyBsaWNlbnNlLiBZb3UgYWxzbyByZXByZXNlbnQKdGhhdCB5b3VyIHN1Ym1pc3Npb24gZG9lcyBub3QsIHRvIHRoZSBiZXN0IG9mIHlvdXIga25vd2xlZGdlLCBpbmZyaW5nZSB1cG9uCmFueW9uZSdzIGNvcHlyaWdodC4KCklmIHRoZSBzdWJtaXNzaW9uIGNvbnRhaW5zIG1hdGVyaWFsIGZvciB3aGljaCB5b3UgZG8gbm90IGhvbGQgY29weXJpZ2h0LAp5b3UgcmVwcmVzZW50IHRoYXQgeW91IGhhdmUgb2J0YWluZWQgdGhlIHVucmVzdHJpY3RlZCBwZXJtaXNzaW9uIG9mIHRoZQpjb3B5cmlnaHQgb3duZXIgdG8gZ3JhbnQgRFNVIHRoZSByaWdodHMgcmVxdWlyZWQgYnkgdGhpcyBsaWNlbnNlLCBhbmQgdGhhdApzdWNoIHRoaXJkLXBhcnR5IG93bmVkIG1hdGVyaWFsIGlzIGNsZWFybHkgaWRlbnRpZmllZCBhbmQgYWNrbm93bGVkZ2VkCndpdGhpbiB0aGUgdGV4dCBvciBjb250ZW50IG9mIHRoZSBzdWJtaXNzaW9uLgoKSUYgVEhFIFNVQk1JU1NJT04gSVMgQkFTRUQgVVBPTiBXT1JLIFRIQVQgSEFTIEJFRU4gU1BPTlNPUkVEIE9SIFNVUFBPUlRFRApCWSBBTiBBR0VOQ1kgT1IgT1JHQU5JWkFUSU9OIE9USEVSIFRIQU4gRFNVLCBZT1UgUkVQUkVTRU5UIFRIQVQgWU9VIEhBVkUKRlVMRklMTEVEIEFOWSBSSUdIVCBPRiBSRVZJRVcgT1IgT1RIRVIgT0JMSUdBVElPTlMgUkVRVUlSRUQgQlkgU1VDSApDT05UUkFDVCBPUiBBR1JFRU1FTlQuCgpEU1Ugd2lsbCBjbGVhcmx5IGlkZW50aWZ5IHlvdXIgbmFtZShzKSBhcyB0aGUgYXV0aG9yKHMpIG9yIG93bmVyKHMpIG9mIHRoZQpzdWJtaXNzaW9uLCBhbmQgd2lsbCBub3QgbWFrZSBhbnkgYWx0ZXJhdGlvbiwgb3RoZXIgdGhhbiBhcyBhbGxvd2VkIGJ5IHRoaXMKbGljZW5zZSwgdG8geW91ciBzdWJtaXNzaW9uLgo=Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452018-04-19T02:00:43LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.en.fl_str_mv |
Somatic embryogenesis in Carica papaya as affected by auxins and explants, and morphoanatomical-related aspects |
title |
Somatic embryogenesis in Carica papaya as affected by auxins and explants, and morphoanatomical-related aspects |
spellingShingle |
Somatic embryogenesis in Carica papaya as affected by auxins and explants, and morphoanatomical-related aspects Cruz, Ana Cláudia F. Carica papaya L. Juvenile explants Maturation Microscopic analysis Synthetic auxins |
title_short |
Somatic embryogenesis in Carica papaya as affected by auxins and explants, and morphoanatomical-related aspects |
title_full |
Somatic embryogenesis in Carica papaya as affected by auxins and explants, and morphoanatomical-related aspects |
title_fullStr |
Somatic embryogenesis in Carica papaya as affected by auxins and explants, and morphoanatomical-related aspects |
title_full_unstemmed |
Somatic embryogenesis in Carica papaya as affected by auxins and explants, and morphoanatomical-related aspects |
title_sort |
Somatic embryogenesis in Carica papaya as affected by auxins and explants, and morphoanatomical-related aspects |
author |
Cruz, Ana Cláudia F. |
author_facet |
Cruz, Ana Cláudia F. Cipriano, Jamile L.D. Mancini, Karina C. Schmildt, Edilson R. Lopes, José Carlos Otoni, Wagner C. Alexandre, Rodrigo S. |
author_role |
author |
author2 |
Cipriano, Jamile L.D. Mancini, Karina C. Schmildt, Edilson R. Lopes, José Carlos Otoni, Wagner C. Alexandre, Rodrigo S. |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Cruz, Ana Cláudia F. Cipriano, Jamile L.D. Mancini, Karina C. Schmildt, Edilson R. Lopes, José Carlos Otoni, Wagner C. Alexandre, Rodrigo S. |
dc.subject.pt-BR.fl_str_mv |
Carica papaya L. Juvenile explants Maturation Microscopic analysis Synthetic auxins |
topic |
Carica papaya L. Juvenile explants Maturation Microscopic analysis Synthetic auxins |
description |
The aim of this study was to evaluate somatic embryogenesis in juvenile explants of the THB papaya cultivar. Apical shoots and cotyledonary leaves were inoculated in an induction medium composed of different concentrations of 2,4-D (6, 9, 12, 15 and 18 µM) or 4-CPA (19, 22, 25, 28 and 31 µM). The embryogenic calluses were transferred to a maturation medium for 30 days. Histological analysis were done during the induction and scanning electron microscopy after maturing. For both types of auxin, embryogenesis was achieved at higher frequencies with cotyledonary leaves incubated in induction medium than with apical shoots; except for callogenesis. The early-stage embryos (e.g., globular or heart-shape) predominated. Among the auxins, best results were observed in cotyledonary leaves induced with 4-CPA (25 µM). Histological analyses of the cotyledonary leaf-derived calluses confirmed that the somatic embryos (SEs) formed from parenchyma cells, predominantly differentiated via indirect and multicellular origin and infrequently via synchronized embryogenesis. The secondary embryogenesis was observed during induction and maturation phases in papaya THB cultivar. The combination of ABA (0.5 µM) and AC (15 g L^-1) in maturation medium resulted in the highest somatic embryogenesis induction frequency (70 SEs callus^-1) and the lowest percentage of early germination (4%). |
publishDate |
2018 |
dc.date.accessioned.fl_str_mv |
2018-04-18T14:09:46Z |
dc.date.available.fl_str_mv |
2018-04-18T14:09:46Z |
dc.date.issued.fl_str_mv |
2018-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/0001-3765201820160252 http://www.locus.ufv.br/handle/123456789/18791 |
dc.identifier.issn.none.fl_str_mv |
16782690 |
identifier_str_mv |
16782690 |
url |
http://dx.doi.org/10.1590/0001-3765201820160252 http://www.locus.ufv.br/handle/123456789/18791 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.pt-BR.fl_str_mv |
v. 90, n. 1, p. 385-400, Março 2018 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Anais da Academia Brasileira de Ciências |
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Anais da Academia Brasileira de Ciências |
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LOCUS Repositório Institucional da UFV |
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