Purificação parcial e caracterização da xilanase produzida por Penicillium expansum

• Autor(a) principal: Querido, André Luiz de Souza
• Data de Publicação: 2002
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/5334
• topic: Xilanase; Penicillium expansum; Resíduos lignocelulósicos; Biotecnologia; Enzima; Purificação; Xylanase; Penicillium expansum; Lignocellulosic wastes; Biotechnology; Enzyme; Purification; CNPQ::CIENCIAS BIOLOGICAS::MICROBIOLOGIA

Penicillium expansum is a filamentous fungus that produces extracelular xylanase. An extracellular xylanase was found to be the major protein in the filtrated culture of P. expansum when grown on 0,3 % wheat bran. In contrast to other microorganism no xilanase multiplicity was found in P. expansum under the conditions used. The used partial purification estrategy was ammonium sulfate fractioning, molecular exclusion cromatography, ultrafiltration and anion exchange chromatography. The proteins eluation profile showed only one form of xylanase that was partially characterized. The optimum pH and temperature were 5,5 and 40 0C, respectively. The enzyme kept stable when preincubed for 1 h under pH between 5.5 and 6.5. It also kept stable when preincubed for 1h under temperature between 20-40 0C. The enzime showed km of 3,03 mg mL-1 and Vmax of 0,027 mmol min-1 μg –1 of protein. The enzymatic activity was increased by 34 % by Mg3(PO4)2 (1 mM); 31 % by MgSO4 (1 mM).and 28 % by Al2(SO4)3 (1 mM).