Proteolytic activity of the nematophagous fungus Arthrobotrys sinensis on Angiostrongylus vasorum larvae
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2014 |
| Outros Autores: | , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | LOCUS Repositório Institucional da UFV |
| Texto Completo: | https://doi.org/10.1186/1756-0500-7-811 http://www.locus.ufv.br/handle/123456789/12535 |
Resumo: | O link do DOI está errado não pode ter escrito periódico capes. |
| id |
UFV_14d8a2e8afc7eff7de710a0f6397cd55 |
|---|---|
| oai_identifier_str |
oai:locus.ufv.br:123456789/12535 |
| network_acronym_str |
UFV |
| network_name_str |
LOCUS Repositório Institucional da UFV |
| repository_id_str |
2145 |
| spelling |
Soares, Filippe Elias de FreitasQueiroz, José Humberto deBraga, Fabio RibeiroAraújo, Jackson Victor deLima, Walter dos SantosZamprogno, Tatiana Tonini2017-10-30T16:43:02Z2017-10-30T16:43:02Z2014-11-181756-0500https://doi.org/10.1186/1756-0500-7-811http://www.locus.ufv.br/handle/123456789/12535O link do DOI está errado não pode ter escrito periódico capes.The predatory nematophagous fungus Arthrobotrys sinensis (SF53) produces three proteases with nematicidal activity when grown on solid media culture. However, the proteolytic profile produced by this fungus, when grown in liquid culture medium remains unknown. Thus, the objective of this work was to evaluate the production of proteases from nematophagous fungus Arthrobotrys sinensis in liquid medium and its nematicidal activity on first stage larvae of A. vasorum. Proteases were obtained in its crude form, using Whatman no.1 filter paper, followed by centrifugation for 5 min at 10 × g and 4°C. A zymogram was performed with co-polymerized casein in an acrylamide gel as substrate. An in vitro assay to evaluate the nematicidal action of the proteases of A. sinensis (SF53) produced in liquid medium on A. vasorum L1 was conducted. By the analysis of the zymogram, it was observed a single halo at the beginning of digestion of the gel, suggesting that the three proteases of SF53 are produced in an enzymatic complex of large molecular weight. Regarding nematicidal activity, within 24 hours, the proteases produced in liquid medium of A. sinensis (SF53) showed a percentage reduction of 64% on the number of L1 of A. vasorum. In the present work, it is suggested that the three proteases of SF53 are produced in an enzymatic complex and was also demonstrated that these enzymes were effective in destroying A. vasorum L1.engBMC Research Notes7:811, November 2014Nematophagous fungiAngiostrongylus vasorumProteaseProteolytic activity of the nematophagous fungus Arthrobotrys sinensis on Angiostrongylus vasorum larvaeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALdocument.pdfdocument.pdfTexto completoapplication/pdf350049https://locus.ufv.br//bitstream/123456789/12535/1/document.pdf225127864418de1d18e79c34f956d10bMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/12535/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILdocument.pdf.jpgdocument.pdf.jpgIM Thumbnailimage/jpeg5887https://locus.ufv.br//bitstream/123456789/12535/3/document.pdf.jpg848fe8e0a5caa940e640cf9b95d53088MD53123456789/125352017-10-30 22:01:03.488oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452017-10-31T01:01:03LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
| dc.title.en.fl_str_mv |
Proteolytic activity of the nematophagous fungus Arthrobotrys sinensis on Angiostrongylus vasorum larvae |
| title |
Proteolytic activity of the nematophagous fungus Arthrobotrys sinensis on Angiostrongylus vasorum larvae |
| spellingShingle |
Proteolytic activity of the nematophagous fungus Arthrobotrys sinensis on Angiostrongylus vasorum larvae Soares, Filippe Elias de Freitas Nematophagous fungi Angiostrongylus vasorum Protease |
| title_short |
Proteolytic activity of the nematophagous fungus Arthrobotrys sinensis on Angiostrongylus vasorum larvae |
| title_full |
Proteolytic activity of the nematophagous fungus Arthrobotrys sinensis on Angiostrongylus vasorum larvae |
| title_fullStr |
Proteolytic activity of the nematophagous fungus Arthrobotrys sinensis on Angiostrongylus vasorum larvae |
| title_full_unstemmed |
Proteolytic activity of the nematophagous fungus Arthrobotrys sinensis on Angiostrongylus vasorum larvae |
| title_sort |
Proteolytic activity of the nematophagous fungus Arthrobotrys sinensis on Angiostrongylus vasorum larvae |
| author |
Soares, Filippe Elias de Freitas |
| author_facet |
Soares, Filippe Elias de Freitas Queiroz, José Humberto de Braga, Fabio Ribeiro Araújo, Jackson Victor de Lima, Walter dos Santos Zamprogno, Tatiana Tonini |
| author_role |
author |
| author2 |
Queiroz, José Humberto de Braga, Fabio Ribeiro Araújo, Jackson Victor de Lima, Walter dos Santos Zamprogno, Tatiana Tonini |
| author2_role |
author author author author author |
| dc.contributor.author.fl_str_mv |
Soares, Filippe Elias de Freitas Queiroz, José Humberto de Braga, Fabio Ribeiro Araújo, Jackson Victor de Lima, Walter dos Santos Zamprogno, Tatiana Tonini |
| dc.subject.pt-BR.fl_str_mv |
Nematophagous fungi Angiostrongylus vasorum Protease |
| topic |
Nematophagous fungi Angiostrongylus vasorum Protease |
| description |
O link do DOI está errado não pode ter escrito periódico capes. |
| publishDate |
2014 |
| dc.date.issued.fl_str_mv |
2014-11-18 |
| dc.date.accessioned.fl_str_mv |
2017-10-30T16:43:02Z |
| dc.date.available.fl_str_mv |
2017-10-30T16:43:02Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
https://doi.org/10.1186/1756-0500-7-811 http://www.locus.ufv.br/handle/123456789/12535 |
| dc.identifier.issn.none.fl_str_mv |
1756-0500 |
| identifier_str_mv |
1756-0500 |
| url |
https://doi.org/10.1186/1756-0500-7-811 http://www.locus.ufv.br/handle/123456789/12535 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartofseries.pt-BR.fl_str_mv |
7:811, November 2014 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
BMC Research Notes |
| publisher.none.fl_str_mv |
BMC Research Notes |
| dc.source.none.fl_str_mv |
reponame:LOCUS Repositório Institucional da UFV instname:Universidade Federal de Viçosa (UFV) instacron:UFV |
| instname_str |
Universidade Federal de Viçosa (UFV) |
| instacron_str |
UFV |
| institution |
UFV |
| reponame_str |
LOCUS Repositório Institucional da UFV |
| collection |
LOCUS Repositório Institucional da UFV |
| bitstream.url.fl_str_mv |
https://locus.ufv.br//bitstream/123456789/12535/1/document.pdf https://locus.ufv.br//bitstream/123456789/12535/2/license.txt https://locus.ufv.br//bitstream/123456789/12535/3/document.pdf.jpg |
| bitstream.checksum.fl_str_mv |
225127864418de1d18e79c34f956d10b 8a4605be74aa9ea9d79846c1fba20a33 848fe8e0a5caa940e640cf9b95d53088 |
| bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
| repository.name.fl_str_mv |
LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV) |
| repository.mail.fl_str_mv |
fabiojreis@ufv.br |
| _version_ |
1801213054255366144 |