The nematophagous fungus Monacrosporium thaumasium and its nematicidal activity on Angiostrongylus vasorum

• Autor(a) principal: Soares, Filippe Elias de Freitas
• Data de Publicação: 2013
• Outros Autores: Braga, Fabio Ribeiro, Araújo, Jackson Victor de, Lima, Walter dos Santos, Queiroz, José Humberto de
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: https://doi.org/10.1016/j.riam.2013.09.005; http://www.locus.ufv.br/handle/123456789/19850
• topic: Nematophagous fungi; Angiostrongylus vasorum; Monacrosporium thaumasium; Protease

The dog acts as a reservoir and environmental disseminator of potentially zoonotic parasites. The objective of this work was to study the fungus Monacrosporium thaumasium regarding its nematicidal potential in laboratory trials and its proteolytic profile. The in vitro test was carried out through two assays (A and B). In assay A, conidia of the fungus N34a were added in positive coprocultures for Angiostrongylus vasorum. In assay B, crude extract (treated group) and distilled water (control group) were added to coprocultures. Next, the proteolytic profile of crude extract of the nematophagous fungus M. thaumasium (NF34a) was revealed by performing a zymogram. There was a reduction (p < 0.01) in the averages of larvae recovered from the treated groups (conidia and crude extract) in relation to control groups. The zymogram suggested that the nematophagous fungus M. thaumasium produces a protease of approximately 40 kDa. The results of this work confirm that the conidia as well as the crude extract of the fungus M. thaumasium may be used to control A. vasorum L1. The proteolytic profile suggested the presence of one protease (Mt1) of approximately 40 kDa that in the future may be used in biological control of L1 of this nematode.