Safety of Lactobacillus plantarum ST8Sh and its bacteriocin

Detalhes bibliográficos
Autor(a) principal: Todorov, Svetoslav Dimitrov
Data de Publicação: 2017
Outros Autores: Perin, Luana M., Nero, Luís Augusto, Carneiro, Bruno M.http://dx.doi.org/10.1007/s12602-017-9260-3, Rahal, Paula, Holzapfel, Wilhelm
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://www.locus.ufv.br/handle/123456789/12883
http://dx.doi.org/10.1007/s12602-017-9260-3
Resumo: Total DNA extracted from Lb. plantarum ST8Sh was screened for the presence of more than 50 genes related to production of biogenic amines (histidine decarboxylase, tyrosine decarboxylase, and ornithine decarboxylase), virulence factors (sex pheromones, gelatinase, cytolysin, hyaluronidase, aggregation substance, enterococcal surface protein, endocarditis antigen, adhesion of collagen, integration factors), and antibiotic resistance (vancomycin, tetracycline, erythromycin, gentamicin, chloramphenicol, bacitracin). Lb. plantarum ST8Sh showed a low presence of virulence genes. Only 13 genes were detected (related to sex pheromones, aggregation substance, adhesion of collagen, tetracycline, gentamicin, chloramphenicol, erythromycin, but not to vancomycin, and bacitracin) and may be considered as indication of safety for application in fermented food products. In addition, interaction between Lb. plantarum ST8Sh and drugs from different groups were determined in order to establish possible application of the strain in combination with commercial drugs. Cytotoxicity of the semi-purified bacteriocins produced by Lb. plantarum ST8Sh was depended on applied concentration—highly cytotoxic when applied at 25 μg/mL and no cytotoxicity at 5 μg/mL.
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spelling Todorov, Svetoslav DimitrovPerin, Luana M.Nero, Luís AugustoCarneiro, Bruno M.http://dx.doi.org/10.1007/s12602-017-9260-3Rahal, PaulaHolzapfel, Wilhelm2017-11-08T12:30:24Z2017-11-08T12:30:24Z2017-02-231867-1314http://www.locus.ufv.br/handle/123456789/12883http://dx.doi.org/10.1007/s12602-017-9260-3Total DNA extracted from Lb. plantarum ST8Sh was screened for the presence of more than 50 genes related to production of biogenic amines (histidine decarboxylase, tyrosine decarboxylase, and ornithine decarboxylase), virulence factors (sex pheromones, gelatinase, cytolysin, hyaluronidase, aggregation substance, enterococcal surface protein, endocarditis antigen, adhesion of collagen, integration factors), and antibiotic resistance (vancomycin, tetracycline, erythromycin, gentamicin, chloramphenicol, bacitracin). Lb. plantarum ST8Sh showed a low presence of virulence genes. Only 13 genes were detected (related to sex pheromones, aggregation substance, adhesion of collagen, tetracycline, gentamicin, chloramphenicol, erythromycin, but not to vancomycin, and bacitracin) and may be considered as indication of safety for application in fermented food products. In addition, interaction between Lb. plantarum ST8Sh and drugs from different groups were determined in order to establish possible application of the strain in combination with commercial drugs. Cytotoxicity of the semi-purified bacteriocins produced by Lb. plantarum ST8Sh was depended on applied concentration—highly cytotoxic when applied at 25 μg/mL and no cytotoxicity at 5 μg/mL.engProbiotics and Antimicrobial ProteinsVolume 9, Issue 3, p. 334–344, September 2017Lactobacillus plantarumProbioticsBacteriocinsSafetyVirulenceCytotoxicitySafety of Lactobacillus plantarum ST8Sh and its bacteriocininfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALs12602-017-9260-3.pdfs12602-017-9260-3.pdftexto completoapplication/pdf461833https://locus.ufv.br//bitstream/123456789/12883/1/s12602-017-9260-3.pdfe1d265ddfdaf65d97837c0e14adae024MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/12883/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILs12602-017-9260-3.pdf.jpgs12602-017-9260-3.pdf.jpgIM Thumbnailimage/jpeg4962https://locus.ufv.br//bitstream/123456789/12883/3/s12602-017-9260-3.pdf.jpgd10ee4a979849f177ec42767d432b25cMD53123456789/128832017-11-08 22:00:23.166oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452017-11-09T01:00:23LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.en.fl_str_mv Safety of Lactobacillus plantarum ST8Sh and its bacteriocin
title Safety of Lactobacillus plantarum ST8Sh and its bacteriocin
spellingShingle Safety of Lactobacillus plantarum ST8Sh and its bacteriocin
Todorov, Svetoslav Dimitrov
Lactobacillus plantarum
Probiotics
Bacteriocins
Safety
Virulence
Cytotoxicity
title_short Safety of Lactobacillus plantarum ST8Sh and its bacteriocin
title_full Safety of Lactobacillus plantarum ST8Sh and its bacteriocin
title_fullStr Safety of Lactobacillus plantarum ST8Sh and its bacteriocin
title_full_unstemmed Safety of Lactobacillus plantarum ST8Sh and its bacteriocin
title_sort Safety of Lactobacillus plantarum ST8Sh and its bacteriocin
author Todorov, Svetoslav Dimitrov
author_facet Todorov, Svetoslav Dimitrov
Perin, Luana M.
Nero, Luís Augusto
Carneiro, Bruno M.http://dx.doi.org/10.1007/s12602-017-9260-3
Rahal, Paula
Holzapfel, Wilhelm
author_role author
author2 Perin, Luana M.
Nero, Luís Augusto
Carneiro, Bruno M.http://dx.doi.org/10.1007/s12602-017-9260-3
Rahal, Paula
Holzapfel, Wilhelm
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Todorov, Svetoslav Dimitrov
Perin, Luana M.
Nero, Luís Augusto
Carneiro, Bruno M.http://dx.doi.org/10.1007/s12602-017-9260-3
Rahal, Paula
Holzapfel, Wilhelm
dc.subject.pt-BR.fl_str_mv Lactobacillus plantarum
Probiotics
Bacteriocins
Safety
Virulence
Cytotoxicity
topic Lactobacillus plantarum
Probiotics
Bacteriocins
Safety
Virulence
Cytotoxicity
description Total DNA extracted from Lb. plantarum ST8Sh was screened for the presence of more than 50 genes related to production of biogenic amines (histidine decarboxylase, tyrosine decarboxylase, and ornithine decarboxylase), virulence factors (sex pheromones, gelatinase, cytolysin, hyaluronidase, aggregation substance, enterococcal surface protein, endocarditis antigen, adhesion of collagen, integration factors), and antibiotic resistance (vancomycin, tetracycline, erythromycin, gentamicin, chloramphenicol, bacitracin). Lb. plantarum ST8Sh showed a low presence of virulence genes. Only 13 genes were detected (related to sex pheromones, aggregation substance, adhesion of collagen, tetracycline, gentamicin, chloramphenicol, erythromycin, but not to vancomycin, and bacitracin) and may be considered as indication of safety for application in fermented food products. In addition, interaction between Lb. plantarum ST8Sh and drugs from different groups were determined in order to establish possible application of the strain in combination with commercial drugs. Cytotoxicity of the semi-purified bacteriocins produced by Lb. plantarum ST8Sh was depended on applied concentration—highly cytotoxic when applied at 25 μg/mL and no cytotoxicity at 5 μg/mL.
publishDate 2017
dc.date.accessioned.fl_str_mv 2017-11-08T12:30:24Z
dc.date.available.fl_str_mv 2017-11-08T12:30:24Z
dc.date.issued.fl_str_mv 2017-02-23
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dc.identifier.issn.none.fl_str_mv 1867-1314
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http://dx.doi.org/10.1007/s12602-017-9260-3
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dc.relation.ispartofseries.pt-BR.fl_str_mv Volume 9, Issue 3, p. 334–344, September 2017
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