Safety of Lactobacillus plantarum ST8Sh and Its Bacteriocin

Detalhes bibliográficos
Autor(a) principal: Todorov, Svetoslav Dimitrov
Data de Publicação: 2017
Outros Autores: Perin, Luana M., Carneiro, Bruno M. [UNESP], Rahal, Paula [UNESP], Holzapfel, Wilhelm, Nero, Luís Augusto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s12602-017-9260-3
http://hdl.handle.net/11449/174264
Resumo: Total DNA extracted from Lb. plantarum ST8Sh was screened for the presence of more than 50 genes related to production of biogenic amines (histidine decarboxylase, tyrosine decarboxylase, and ornithine decarboxylase), virulence factors (sex pheromones, gelatinase, cytolysin, hyaluronidase, aggregation substance, enterococcal surface protein, endocarditis antigen, adhesion of collagen, integration factors), and antibiotic resistance (vancomycin, tetracycline, erythromycin, gentamicin, chloramphenicol, bacitracin). Lb. plantarum ST8Sh showed a low presence of virulence genes. Only 13 genes were detected (related to sex pheromones, aggregation substance, adhesion of collagen, tetracycline, gentamicin, chloramphenicol, erythromycin, but not to vancomycin, and bacitracin) and may be considered as indication of safety for application in fermented food products. In addition, interaction between Lb. plantarum ST8Sh and drugs from different groups were determined in order to establish possible application of the strain in combination with commercial drugs. Cytotoxicity of the semi-purified bacteriocins produced by Lb. plantarum ST8Sh was depended on applied concentration—highly cytotoxic when applied at 25 μg/mL and no cytotoxicity at 5 μg/mL.
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spelling Safety of Lactobacillus plantarum ST8Sh and Its BacteriocinBacteriocinsCytotoxicityLactobacillus plantarumProbioticsSafetyVirulenceTotal DNA extracted from Lb. plantarum ST8Sh was screened for the presence of more than 50 genes related to production of biogenic amines (histidine decarboxylase, tyrosine decarboxylase, and ornithine decarboxylase), virulence factors (sex pheromones, gelatinase, cytolysin, hyaluronidase, aggregation substance, enterococcal surface protein, endocarditis antigen, adhesion of collagen, integration factors), and antibiotic resistance (vancomycin, tetracycline, erythromycin, gentamicin, chloramphenicol, bacitracin). Lb. plantarum ST8Sh showed a low presence of virulence genes. Only 13 genes were detected (related to sex pheromones, aggregation substance, adhesion of collagen, tetracycline, gentamicin, chloramphenicol, erythromycin, but not to vancomycin, and bacitracin) and may be considered as indication of safety for application in fermented food products. In addition, interaction between Lb. plantarum ST8Sh and drugs from different groups were determined in order to establish possible application of the strain in combination with commercial drugs. Cytotoxicity of the semi-purified bacteriocins produced by Lb. plantarum ST8Sh was depended on applied concentration—highly cytotoxic when applied at 25 μg/mL and no cytotoxicity at 5 μg/mL.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Veterinary Department Universidade Federal de Viçosa, Campus UFVDepartamento de Engenharia e Tecnologia de Alimentos UNESP - Universidade Estadual Paulista Instituto de Biociências Letras e Ciências ExatasInstituto de Ciências Exatas e Naturais Universidade Federal de Mato GrossoGEE Handong Global UniversityDepartamento de Engenharia e Tecnologia de Alimentos UNESP - Universidade Estadual Paulista Instituto de Biociências Letras e Ciências ExatasUniversidade Federal de Viçosa (UFV)Universidade Estadual Paulista (Unesp)Universidade Federal de Mato GrossoHandong Global UniversityTodorov, Svetoslav DimitrovPerin, Luana M.Carneiro, Bruno M. [UNESP]Rahal, Paula [UNESP]Holzapfel, WilhelmNero, Luís Augusto2018-12-11T17:10:05Z2018-12-11T17:10:05Z2017-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article334-344application/pdfhttp://dx.doi.org/10.1007/s12602-017-9260-3Probiotics and Antimicrobial Proteins, v. 9, n. 3, p. 334-344, 2017.1867-1306http://hdl.handle.net/11449/17426410.1007/s12602-017-9260-32-s2.0-850136587572-s2.0-85013658757.pdf79910823626712120000-0001-5693-6148Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengProbiotics and Antimicrobial Proteins0,692info:eu-repo/semantics/openAccess2023-11-08T06:07:10Zoai:repositorio.unesp.br:11449/174264Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:07:48.409960Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Safety of Lactobacillus plantarum ST8Sh and Its Bacteriocin
title Safety of Lactobacillus plantarum ST8Sh and Its Bacteriocin
spellingShingle Safety of Lactobacillus plantarum ST8Sh and Its Bacteriocin
Todorov, Svetoslav Dimitrov
Bacteriocins
Cytotoxicity
Lactobacillus plantarum
Probiotics
Safety
Virulence
title_short Safety of Lactobacillus plantarum ST8Sh and Its Bacteriocin
title_full Safety of Lactobacillus plantarum ST8Sh and Its Bacteriocin
title_fullStr Safety of Lactobacillus plantarum ST8Sh and Its Bacteriocin
title_full_unstemmed Safety of Lactobacillus plantarum ST8Sh and Its Bacteriocin
title_sort Safety of Lactobacillus plantarum ST8Sh and Its Bacteriocin
author Todorov, Svetoslav Dimitrov
author_facet Todorov, Svetoslav Dimitrov
Perin, Luana M.
Carneiro, Bruno M. [UNESP]
Rahal, Paula [UNESP]
Holzapfel, Wilhelm
Nero, Luís Augusto
author_role author
author2 Perin, Luana M.
Carneiro, Bruno M. [UNESP]
Rahal, Paula [UNESP]
Holzapfel, Wilhelm
Nero, Luís Augusto
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de Viçosa (UFV)
Universidade Estadual Paulista (Unesp)
Universidade Federal de Mato Grosso
Handong Global University
dc.contributor.author.fl_str_mv Todorov, Svetoslav Dimitrov
Perin, Luana M.
Carneiro, Bruno M. [UNESP]
Rahal, Paula [UNESP]
Holzapfel, Wilhelm
Nero, Luís Augusto
dc.subject.por.fl_str_mv Bacteriocins
Cytotoxicity
Lactobacillus plantarum
Probiotics
Safety
Virulence
topic Bacteriocins
Cytotoxicity
Lactobacillus plantarum
Probiotics
Safety
Virulence
description Total DNA extracted from Lb. plantarum ST8Sh was screened for the presence of more than 50 genes related to production of biogenic amines (histidine decarboxylase, tyrosine decarboxylase, and ornithine decarboxylase), virulence factors (sex pheromones, gelatinase, cytolysin, hyaluronidase, aggregation substance, enterococcal surface protein, endocarditis antigen, adhesion of collagen, integration factors), and antibiotic resistance (vancomycin, tetracycline, erythromycin, gentamicin, chloramphenicol, bacitracin). Lb. plantarum ST8Sh showed a low presence of virulence genes. Only 13 genes were detected (related to sex pheromones, aggregation substance, adhesion of collagen, tetracycline, gentamicin, chloramphenicol, erythromycin, but not to vancomycin, and bacitracin) and may be considered as indication of safety for application in fermented food products. In addition, interaction between Lb. plantarum ST8Sh and drugs from different groups were determined in order to establish possible application of the strain in combination with commercial drugs. Cytotoxicity of the semi-purified bacteriocins produced by Lb. plantarum ST8Sh was depended on applied concentration—highly cytotoxic when applied at 25 μg/mL and no cytotoxicity at 5 μg/mL.
publishDate 2017
dc.date.none.fl_str_mv 2017-09-01
2018-12-11T17:10:05Z
2018-12-11T17:10:05Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s12602-017-9260-3
Probiotics and Antimicrobial Proteins, v. 9, n. 3, p. 334-344, 2017.
1867-1306
http://hdl.handle.net/11449/174264
10.1007/s12602-017-9260-3
2-s2.0-85013658757
2-s2.0-85013658757.pdf
7991082362671212
0000-0001-5693-6148
url http://dx.doi.org/10.1007/s12602-017-9260-3
http://hdl.handle.net/11449/174264
identifier_str_mv Probiotics and Antimicrobial Proteins, v. 9, n. 3, p. 334-344, 2017.
1867-1306
10.1007/s12602-017-9260-3
2-s2.0-85013658757
2-s2.0-85013658757.pdf
7991082362671212
0000-0001-5693-6148
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Probiotics and Antimicrobial Proteins
0,692
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 334-344
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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