PacCl, a pH-responsive transcriptional regulator, is essential in the pathogenicity of Colletotrichum lindemuthianum, a causal agent of anthracnose in bean plants

Detalhes bibliográficos
Autor(a) principal: Nogueira, Guilherme Bicalho
Data de Publicação: 2014
Outros Autores: Soares, Marcos Antônio, Bazzolli, Denise Mara Soares, Araújo, Elza Fernandes de, Langin, Thierry, Queiroz, Marisa Vieira de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://dx.doi.org/10.1007/s10658-014-0508-4
http://www.locus.ufv.br/handle/123456789/22302
Resumo: In fungi, the expression of genes encoding proteins related to parasitism is regulated by several factors, including pH. This study reports the structural and functional characterization of the pacCl gene, which encodes the transcription factor PacC of C. lindemuthianum. The pacCl gene showed reduced expression in acidic pH, and its transcription was activated by elevated extracellular pH. The importance of this gene was demonstrated by the development of a pacC1 disruption mutant line of C. lindemuthianum. The mutant line was able to penetrate the host tissue through differentiation of primary hyphae. However, it was not able to cause maceration on the infected plant tissue. The results suggest that PacCl is a regulator of gene activation, and its expression is required for fungal growth in alkaline conditions, as well as for the transcription of genes necessary for the passage from the biotrophic to the necrotrophic phase.
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spelling PacCl, a pH-responsive transcriptional regulator, is essential in the pathogenicity of Colletotrichum lindemuthianum, a causal agent of anthracnose in bean plantsColletotrichum lindemuthianumAnthracnosepHPacCIn fungi, the expression of genes encoding proteins related to parasitism is regulated by several factors, including pH. This study reports the structural and functional characterization of the pacCl gene, which encodes the transcription factor PacC of C. lindemuthianum. The pacCl gene showed reduced expression in acidic pH, and its transcription was activated by elevated extracellular pH. The importance of this gene was demonstrated by the development of a pacC1 disruption mutant line of C. lindemuthianum. The mutant line was able to penetrate the host tissue through differentiation of primary hyphae. However, it was not able to cause maceration on the infected plant tissue. The results suggest that PacCl is a regulator of gene activation, and its expression is required for fungal growth in alkaline conditions, as well as for the transcription of genes necessary for the passage from the biotrophic to the necrotrophic phase.European Journal of Plant Pathology2018-10-17T10:37:05Z2018-10-17T10:37:05Z2014-08-08info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlepdfapplication/pdf15738469http://dx.doi.org/10.1007/s10658-014-0508-4http://www.locus.ufv.br/handle/123456789/22302engv. 140, n. 4, p. 769– 785, dez. 2014Springer Nature Switzerland AG.info:eu-repo/semantics/openAccessNogueira, Guilherme BicalhoSoares, Marcos AntônioBazzolli, Denise Mara SoaresAraújo, Elza Fernandes deLangin, ThierryQueiroz, Marisa Vieira dereponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFV2024-07-12T08:39:16Zoai:locus.ufv.br:123456789/22302Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452024-07-12T08:39:16LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.none.fl_str_mv PacCl, a pH-responsive transcriptional regulator, is essential in the pathogenicity of Colletotrichum lindemuthianum, a causal agent of anthracnose in bean plants
title PacCl, a pH-responsive transcriptional regulator, is essential in the pathogenicity of Colletotrichum lindemuthianum, a causal agent of anthracnose in bean plants
spellingShingle PacCl, a pH-responsive transcriptional regulator, is essential in the pathogenicity of Colletotrichum lindemuthianum, a causal agent of anthracnose in bean plants
Nogueira, Guilherme Bicalho
Colletotrichum lindemuthianum
Anthracnose
pH
PacC
title_short PacCl, a pH-responsive transcriptional regulator, is essential in the pathogenicity of Colletotrichum lindemuthianum, a causal agent of anthracnose in bean plants
title_full PacCl, a pH-responsive transcriptional regulator, is essential in the pathogenicity of Colletotrichum lindemuthianum, a causal agent of anthracnose in bean plants
title_fullStr PacCl, a pH-responsive transcriptional regulator, is essential in the pathogenicity of Colletotrichum lindemuthianum, a causal agent of anthracnose in bean plants
title_full_unstemmed PacCl, a pH-responsive transcriptional regulator, is essential in the pathogenicity of Colletotrichum lindemuthianum, a causal agent of anthracnose in bean plants
title_sort PacCl, a pH-responsive transcriptional regulator, is essential in the pathogenicity of Colletotrichum lindemuthianum, a causal agent of anthracnose in bean plants
author Nogueira, Guilherme Bicalho
author_facet Nogueira, Guilherme Bicalho
Soares, Marcos Antônio
Bazzolli, Denise Mara Soares
Araújo, Elza Fernandes de
Langin, Thierry
Queiroz, Marisa Vieira de
author_role author
author2 Soares, Marcos Antônio
Bazzolli, Denise Mara Soares
Araújo, Elza Fernandes de
Langin, Thierry
Queiroz, Marisa Vieira de
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Nogueira, Guilherme Bicalho
Soares, Marcos Antônio
Bazzolli, Denise Mara Soares
Araújo, Elza Fernandes de
Langin, Thierry
Queiroz, Marisa Vieira de
dc.subject.por.fl_str_mv Colletotrichum lindemuthianum
Anthracnose
pH
PacC
topic Colletotrichum lindemuthianum
Anthracnose
pH
PacC
description In fungi, the expression of genes encoding proteins related to parasitism is regulated by several factors, including pH. This study reports the structural and functional characterization of the pacCl gene, which encodes the transcription factor PacC of C. lindemuthianum. The pacCl gene showed reduced expression in acidic pH, and its transcription was activated by elevated extracellular pH. The importance of this gene was demonstrated by the development of a pacC1 disruption mutant line of C. lindemuthianum. The mutant line was able to penetrate the host tissue through differentiation of primary hyphae. However, it was not able to cause maceration on the infected plant tissue. The results suggest that PacCl is a regulator of gene activation, and its expression is required for fungal growth in alkaline conditions, as well as for the transcription of genes necessary for the passage from the biotrophic to the necrotrophic phase.
publishDate 2014
dc.date.none.fl_str_mv 2014-08-08
2018-10-17T10:37:05Z
2018-10-17T10:37:05Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv 15738469
http://dx.doi.org/10.1007/s10658-014-0508-4
http://www.locus.ufv.br/handle/123456789/22302
identifier_str_mv 15738469
url http://dx.doi.org/10.1007/s10658-014-0508-4
http://www.locus.ufv.br/handle/123456789/22302
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv v. 140, n. 4, p. 769– 785, dez. 2014
dc.rights.driver.fl_str_mv Springer Nature Switzerland AG.
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Springer Nature Switzerland AG.
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv pdf
application/pdf
dc.publisher.none.fl_str_mv European Journal of Plant Pathology
publisher.none.fl_str_mv European Journal of Plant Pathology
dc.source.none.fl_str_mv reponame:LOCUS Repositório Institucional da UFV
instname:Universidade Federal de Viçosa (UFV)
instacron:UFV
instname_str Universidade Federal de Viçosa (UFV)
instacron_str UFV
institution UFV
reponame_str LOCUS Repositório Institucional da UFV
collection LOCUS Repositório Institucional da UFV
repository.name.fl_str_mv LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)
repository.mail.fl_str_mv fabiojreis@ufv.br
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