PacCl, a pH-responsive transcriptional regulator, is essential in the pathogenicity of Colletotrichum lindemuthianum, a causal agent of anthracnose in bean plants
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | http://dx.doi.org/10.1007/s10658-014-0508-4 http://www.locus.ufv.br/handle/123456789/22302 |
Resumo: | In fungi, the expression of genes encoding proteins related to parasitism is regulated by several factors, including pH. This study reports the structural and functional characterization of the pacCl gene, which encodes the transcription factor PacC of C. lindemuthianum. The pacCl gene showed reduced expression in acidic pH, and its transcription was activated by elevated extracellular pH. The importance of this gene was demonstrated by the development of a pacC1 disruption mutant line of C. lindemuthianum. The mutant line was able to penetrate the host tissue through differentiation of primary hyphae. However, it was not able to cause maceration on the infected plant tissue. The results suggest that PacCl is a regulator of gene activation, and its expression is required for fungal growth in alkaline conditions, as well as for the transcription of genes necessary for the passage from the biotrophic to the necrotrophic phase. |
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PacCl, a pH-responsive transcriptional regulator, is essential in the pathogenicity of Colletotrichum lindemuthianum, a causal agent of anthracnose in bean plantsColletotrichum lindemuthianumAnthracnosepHPacCIn fungi, the expression of genes encoding proteins related to parasitism is regulated by several factors, including pH. This study reports the structural and functional characterization of the pacCl gene, which encodes the transcription factor PacC of C. lindemuthianum. The pacCl gene showed reduced expression in acidic pH, and its transcription was activated by elevated extracellular pH. The importance of this gene was demonstrated by the development of a pacC1 disruption mutant line of C. lindemuthianum. The mutant line was able to penetrate the host tissue through differentiation of primary hyphae. However, it was not able to cause maceration on the infected plant tissue. The results suggest that PacCl is a regulator of gene activation, and its expression is required for fungal growth in alkaline conditions, as well as for the transcription of genes necessary for the passage from the biotrophic to the necrotrophic phase.European Journal of Plant Pathology2018-10-17T10:37:05Z2018-10-17T10:37:05Z2014-08-08info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlepdfapplication/pdf15738469http://dx.doi.org/10.1007/s10658-014-0508-4http://www.locus.ufv.br/handle/123456789/22302engv. 140, n. 4, p. 769– 785, dez. 2014Springer Nature Switzerland AG.info:eu-repo/semantics/openAccessNogueira, Guilherme BicalhoSoares, Marcos AntônioBazzolli, Denise Mara SoaresAraújo, Elza Fernandes deLangin, ThierryQueiroz, Marisa Vieira dereponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFV2024-07-12T08:39:16Zoai:locus.ufv.br:123456789/22302Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452024-07-12T08:39:16LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.none.fl_str_mv |
PacCl, a pH-responsive transcriptional regulator, is essential in the pathogenicity of Colletotrichum lindemuthianum, a causal agent of anthracnose in bean plants |
title |
PacCl, a pH-responsive transcriptional regulator, is essential in the pathogenicity of Colletotrichum lindemuthianum, a causal agent of anthracnose in bean plants |
spellingShingle |
PacCl, a pH-responsive transcriptional regulator, is essential in the pathogenicity of Colletotrichum lindemuthianum, a causal agent of anthracnose in bean plants Nogueira, Guilherme Bicalho Colletotrichum lindemuthianum Anthracnose pH PacC |
title_short |
PacCl, a pH-responsive transcriptional regulator, is essential in the pathogenicity of Colletotrichum lindemuthianum, a causal agent of anthracnose in bean plants |
title_full |
PacCl, a pH-responsive transcriptional regulator, is essential in the pathogenicity of Colletotrichum lindemuthianum, a causal agent of anthracnose in bean plants |
title_fullStr |
PacCl, a pH-responsive transcriptional regulator, is essential in the pathogenicity of Colletotrichum lindemuthianum, a causal agent of anthracnose in bean plants |
title_full_unstemmed |
PacCl, a pH-responsive transcriptional regulator, is essential in the pathogenicity of Colletotrichum lindemuthianum, a causal agent of anthracnose in bean plants |
title_sort |
PacCl, a pH-responsive transcriptional regulator, is essential in the pathogenicity of Colletotrichum lindemuthianum, a causal agent of anthracnose in bean plants |
author |
Nogueira, Guilherme Bicalho |
author_facet |
Nogueira, Guilherme Bicalho Soares, Marcos Antônio Bazzolli, Denise Mara Soares Araújo, Elza Fernandes de Langin, Thierry Queiroz, Marisa Vieira de |
author_role |
author |
author2 |
Soares, Marcos Antônio Bazzolli, Denise Mara Soares Araújo, Elza Fernandes de Langin, Thierry Queiroz, Marisa Vieira de |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Nogueira, Guilherme Bicalho Soares, Marcos Antônio Bazzolli, Denise Mara Soares Araújo, Elza Fernandes de Langin, Thierry Queiroz, Marisa Vieira de |
dc.subject.por.fl_str_mv |
Colletotrichum lindemuthianum Anthracnose pH PacC |
topic |
Colletotrichum lindemuthianum Anthracnose pH PacC |
description |
In fungi, the expression of genes encoding proteins related to parasitism is regulated by several factors, including pH. This study reports the structural and functional characterization of the pacCl gene, which encodes the transcription factor PacC of C. lindemuthianum. The pacCl gene showed reduced expression in acidic pH, and its transcription was activated by elevated extracellular pH. The importance of this gene was demonstrated by the development of a pacC1 disruption mutant line of C. lindemuthianum. The mutant line was able to penetrate the host tissue through differentiation of primary hyphae. However, it was not able to cause maceration on the infected plant tissue. The results suggest that PacCl is a regulator of gene activation, and its expression is required for fungal growth in alkaline conditions, as well as for the transcription of genes necessary for the passage from the biotrophic to the necrotrophic phase. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-08-08 2018-10-17T10:37:05Z 2018-10-17T10:37:05Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
15738469 http://dx.doi.org/10.1007/s10658-014-0508-4 http://www.locus.ufv.br/handle/123456789/22302 |
identifier_str_mv |
15738469 |
url |
http://dx.doi.org/10.1007/s10658-014-0508-4 http://www.locus.ufv.br/handle/123456789/22302 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
v. 140, n. 4, p. 769– 785, dez. 2014 |
dc.rights.driver.fl_str_mv |
Springer Nature Switzerland AG. info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Springer Nature Switzerland AG. |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
pdf application/pdf |
dc.publisher.none.fl_str_mv |
European Journal of Plant Pathology |
publisher.none.fl_str_mv |
European Journal of Plant Pathology |
dc.source.none.fl_str_mv |
reponame:LOCUS Repositório Institucional da UFV instname:Universidade Federal de Viçosa (UFV) instacron:UFV |
instname_str |
Universidade Federal de Viçosa (UFV) |
instacron_str |
UFV |
institution |
UFV |
reponame_str |
LOCUS Repositório Institucional da UFV |
collection |
LOCUS Repositório Institucional da UFV |
repository.name.fl_str_mv |
LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV) |
repository.mail.fl_str_mv |
fabiojreis@ufv.br |
_version_ |
1817560032342966272 |