Celulases e hemicelulases de espécies endofíticas de Acremonium

• Autor(a) principal: Almeida, Maíra Nicolau de
• Data de Publicação: 2009
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/2407
• topic: Celulases; Hemicelulases; Acremonium; Cellulases; Hemicellulases; Acremonium; CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::ENZIMOLOGIA

Two Acremonium species (Acremonium zeae EA0802 and Acremonium sp. EA0810) were examined in relation to their ability to produce cellulases (FPases), endoglucanases, β- glucosidases, xylanases, α-galactosidases, α- arabinofuranosidases and β-xylosidases in different carbon sources. The fungi were cultivated in submerged culture containing L-arabinose, D-xylose, oat spelt xylan, sugar cane bagasse or corn straw as a carbon source and aliquots were analyzed daily for a period of 23 days to verify enzyme production. Enzyme production in solid state fermentation utilizing sugar cane bagasse or corn straw as a carbon source and support was also tested. In this case, enzyme production was analyzed at 3 day intervals for a period of 39 days. The highest FPase, endoglucanase and xylanase activities were obtained when Acremonium sp. EA0810 and Acremonium zeae EA0802 were cultivated in submerged culture containing sugar cane bagasse and corn straw as a carbon source. Acremonium sp. EA0810 show the highest β-glucosidase activity when it was cultivated in submerged culture using D-xylose as carbon source. Acremonium zeae EA0802 exhibit greatest α-arabinofuranosidase and α-galactosidase activities when it was cultivated in submerged culture utilizing xylan as a carbon source. FPase, endoglucanase, β-glucosidase and xylanase from Acremonium sp. EA0810 were found to have optimum pH and temperatures of 6.0, 55 °C; 5.0, 70 ºC; 4.5, 60 °C and 6.5, 50 °C, respectively. α-Arabinofuranosidase and α-galactosidase from Acremonium zeae EA0802 have optimum pH and temperatures of 5.0, 60 °C and 4.5, 45 °C, respectively. An enzymatic extract containing endoglucanase and xylanase activities was submitted to a zymogram analyze and one form of each enzyme was detected. Endoglucanase biochemical characteristics are appropriate for industrial application.