Comparative study on the use of specific and heterologous microsatellite primers in the stingless bees Melipona rufiventris and M. mondury (Hymenoptera, Apidae)

Detalhes bibliográficos
Autor(a) principal: Lopes, Denilce Meneses
Data de Publicação: 2009
Outros Autores: Campos, Lúcio Antônio de Oliveira, Salomão, Tânia Maria Fernandes, Tavares, Mara Garcia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://dx.doi.org/10.1590/S1415-47572010005000017
http://www.locus.ufv.br/handle/123456789/12290
Resumo: Due to their high degree of polymorphism, microsatellites are considered useful tools for studying population genetics. Nevertheless, studies of genetic diversity in stingless bees by means of these primers have revealed a low level of polymorphism, possibly the consequence of the heterologous primers used, since in most cases these were not specifically designed for the species under consideration. Herein we compared the number of polymorphic loci and alleles per locus, as well as observed heterozygosity in Melipona rufiventris and M. mondury populations, using specific and heterologous primers. The use of specific primers placed in evidence the greater frequency of polymorphic loci and alleles per locus, besides an expressive increase in observed heterozygosity in M. rufiventris and M. mondury, thereby reinforcing the idea that populational studies should be undertaken by preferably using species-specific microsatellite primers.
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spelling Comparative study on the use of specific and heterologous microsatellite primers in the stingless bees Melipona rufiventris and M. mondury (Hymenoptera, Apidae)MeliponaStingless beesMicrosatelliteTransferabilityDue to their high degree of polymorphism, microsatellites are considered useful tools for studying population genetics. Nevertheless, studies of genetic diversity in stingless bees by means of these primers have revealed a low level of polymorphism, possibly the consequence of the heterologous primers used, since in most cases these were not specifically designed for the species under consideration. Herein we compared the number of polymorphic loci and alleles per locus, as well as observed heterozygosity in Melipona rufiventris and M. mondury populations, using specific and heterologous primers. The use of specific primers placed in evidence the greater frequency of polymorphic loci and alleles per locus, besides an expressive increase in observed heterozygosity in M. rufiventris and M. mondury, thereby reinforcing the idea that populational studies should be undertaken by preferably using species-specific microsatellite primers.Genetics and Molecular Biology2017-10-23T17:05:15Z2017-10-23T17:05:15Z2009-10-14info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlepdfapplication/pdf1415-4757http://dx.doi.org/10.1590/S1415-47572010005000017http://www.locus.ufv.br/handle/123456789/12290eng33(2) p.390-393, Apr-Jun 2010Lopes, Denilce MenesesCampos, Lúcio Antônio de OliveiraSalomão, Tânia Maria FernandesTavares, Mara Garciainfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFV2024-07-12T08:08:29Zoai:locus.ufv.br:123456789/12290Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452024-07-12T08:08:29LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.none.fl_str_mv Comparative study on the use of specific and heterologous microsatellite primers in the stingless bees Melipona rufiventris and M. mondury (Hymenoptera, Apidae)
title Comparative study on the use of specific and heterologous microsatellite primers in the stingless bees Melipona rufiventris and M. mondury (Hymenoptera, Apidae)
spellingShingle Comparative study on the use of specific and heterologous microsatellite primers in the stingless bees Melipona rufiventris and M. mondury (Hymenoptera, Apidae)
Lopes, Denilce Meneses
Melipona
Stingless bees
Microsatellite
Transferability
title_short Comparative study on the use of specific and heterologous microsatellite primers in the stingless bees Melipona rufiventris and M. mondury (Hymenoptera, Apidae)
title_full Comparative study on the use of specific and heterologous microsatellite primers in the stingless bees Melipona rufiventris and M. mondury (Hymenoptera, Apidae)
title_fullStr Comparative study on the use of specific and heterologous microsatellite primers in the stingless bees Melipona rufiventris and M. mondury (Hymenoptera, Apidae)
title_full_unstemmed Comparative study on the use of specific and heterologous microsatellite primers in the stingless bees Melipona rufiventris and M. mondury (Hymenoptera, Apidae)
title_sort Comparative study on the use of specific and heterologous microsatellite primers in the stingless bees Melipona rufiventris and M. mondury (Hymenoptera, Apidae)
author Lopes, Denilce Meneses
author_facet Lopes, Denilce Meneses
Campos, Lúcio Antônio de Oliveira
Salomão, Tânia Maria Fernandes
Tavares, Mara Garcia
author_role author
author2 Campos, Lúcio Antônio de Oliveira
Salomão, Tânia Maria Fernandes
Tavares, Mara Garcia
author2_role author
author
author
dc.contributor.author.fl_str_mv Lopes, Denilce Meneses
Campos, Lúcio Antônio de Oliveira
Salomão, Tânia Maria Fernandes
Tavares, Mara Garcia
dc.subject.por.fl_str_mv Melipona
Stingless bees
Microsatellite
Transferability
topic Melipona
Stingless bees
Microsatellite
Transferability
description Due to their high degree of polymorphism, microsatellites are considered useful tools for studying population genetics. Nevertheless, studies of genetic diversity in stingless bees by means of these primers have revealed a low level of polymorphism, possibly the consequence of the heterologous primers used, since in most cases these were not specifically designed for the species under consideration. Herein we compared the number of polymorphic loci and alleles per locus, as well as observed heterozygosity in Melipona rufiventris and M. mondury populations, using specific and heterologous primers. The use of specific primers placed in evidence the greater frequency of polymorphic loci and alleles per locus, besides an expressive increase in observed heterozygosity in M. rufiventris and M. mondury, thereby reinforcing the idea that populational studies should be undertaken by preferably using species-specific microsatellite primers.
publishDate 2009
dc.date.none.fl_str_mv 2009-10-14
2017-10-23T17:05:15Z
2017-10-23T17:05:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv 1415-4757
http://dx.doi.org/10.1590/S1415-47572010005000017
http://www.locus.ufv.br/handle/123456789/12290
identifier_str_mv 1415-4757
url http://dx.doi.org/10.1590/S1415-47572010005000017
http://www.locus.ufv.br/handle/123456789/12290
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 33(2) p.390-393, Apr-Jun 2010
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv pdf
application/pdf
dc.publisher.none.fl_str_mv Genetics and Molecular Biology
publisher.none.fl_str_mv Genetics and Molecular Biology
dc.source.none.fl_str_mv reponame:LOCUS Repositório Institucional da UFV
instname:Universidade Federal de Viçosa (UFV)
instacron:UFV
instname_str Universidade Federal de Viçosa (UFV)
instacron_str UFV
institution UFV
reponame_str LOCUS Repositório Institucional da UFV
collection LOCUS Repositório Institucional da UFV
repository.name.fl_str_mv LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)
repository.mail.fl_str_mv fabiojreis@ufv.br
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