Incidência e transmissão de dsRNA em Pseudocercospora griseola, agente causal da mancha-angular do feijoeiro comum (Phaseolus vulgaris)

Detalhes bibliográficos
Autor(a) principal: Lima, Swiany Silveira
Data de Publicação: 2008
Tipo de documento: Dissertação
Idioma: por
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://locus.ufv.br/handle/123456789/5300
Resumo: The common bean Phaseolus vulgaris shows great importance under feeding and economical aspects for the Brazilian people. However, its productivity has been low due to the occurrence of diseases and other factors. The angular leaf spot is distinguished among those diseases. Its causal agent is the Pseudocercospora griseola (Sacc.) Crous & U. Braun. Some mycovirus or virus-like particles were already described in several phytopathogenic fungus. Those viruses are unable to penetrating and lysing the host cells, and the intracytoplasmic transmission is accomplished by anastomosis among hyphae and the sporogenesis. Most mycovirus are found as multiple dsRNA fragments. In general, the mycovirus are cryptic (latent) concerning to the effects caused into phenotype of the host fungus, but they can affect the biology of their host by provoking morphological changes, hyper or hypovirulence. Because they are associated to the hypovirulence phenomenon, the mycoviruses show a potential use in the biocontroll of the phytopathogenic fungus. The general objective of this work was to characterize the mycovirus in the isolates of P. griseola, since they were recently detected in this fungus species for the first time. To reach this objective, the following were performed: the characterization of the dsRNA in different isolates; the vertical transmission analysis; and the obtainment of isogenic lines by the virus cure. The dsRNAs were detected in 31 from those 49 isolates of P. griseola under analysis. In the present study, most isolates showed multiple dsRNA fragments varying from zero to 10, as being the sizes estimated between 0.8 and 4.8 kb. The dsRNA fragment of 4.8 kb from the isolate was efficiently transmitted to the asexual spores. However, not all dsRNA fragments (between 1 and 6) found in the isolate Ig848 were transmitted to monosporic colonies. The cycloheximide was used at concentration of 20 µg/mL in order to obtain the mycovirus cure. This treatment was ineffective for the isolate 29-3, since those three colonies transplanted to cyclohexymide during four generations showed the same profile as the total nucleic acids found in the wild isolate. In the case of the isolate Ig848, this same chemical treatment eliminated the fragments 2.2; 2.0; 1.8; 1.2 and 1.0 kb of the colonies Ch2 and Ch4 after seven successive transplantings in medium containing cycloheximide. Several phytopathogenic fungus are attacked by viral infections, and this variation in the profile of the nucleic acid found in the P. griseola isolates is also observed in other plant pathogens. The presence of multiple fragments in only one isolate may be due to the infection by virus with segmented genome, RNA satellite, defective RNA or mixed infections. The efficiency of the transmission by conidia is variable, as depending on the fungus species under consideration, but it is usually near 100% as occurred for the isolate 29-3 of P. griseola. Either cure of some dsRNA fragments and the spontaneous loss during conidiogenesis observed for the isolate Ig848 rather indicate the infection by independent replicons. In those isogenic lines with and without some dsRNA fragments, the effect of the viral infection will be evaluated under some aspects such as the sporulation rate, growth and pathogenicity. The characterization of those viruses found in P. griseola will allow for further studies concerning to their use in the biological control of the angular leaf spot, which will turn possible to reduce the economical losses caused by this disease in agriculture.
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spelling Lima, Swiany Silveirahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4190755A5Araujo, Elza Fernandes dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783675E2Zerbini Júnior, Francisco Murilohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783743U5Queiroz, Marisa Vieira dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785812Z5Kasuya, Maria Catarina Megumihttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4721444T5Barros, Everaldo Gonçalves dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781285J6Costa, Maurício Dutrahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4728228J52015-03-26T13:51:44Z2009-02-122015-03-26T13:51:44Z2008-07-29LIMA, Swiany Silveira. Incidence and transmission of dsRNA in Phaeoisariopsis griseola, the agent causing the angular leaf spot in the common bean (Phaseolus vulgaris). 2008. 5 f. Dissertação (Mestrado em Associações micorrízicas; Bactérias láticas e probióticos; Biologia molecular de fungos de interesse) - Universidade Federal de Viçosa, Viçosa, 2008.http://locus.ufv.br/handle/123456789/5300The common bean Phaseolus vulgaris shows great importance under feeding and economical aspects for the Brazilian people. However, its productivity has been low due to the occurrence of diseases and other factors. The angular leaf spot is distinguished among those diseases. Its causal agent is the Pseudocercospora griseola (Sacc.) Crous & U. Braun. Some mycovirus or virus-like particles were already described in several phytopathogenic fungus. Those viruses are unable to penetrating and lysing the host cells, and the intracytoplasmic transmission is accomplished by anastomosis among hyphae and the sporogenesis. Most mycovirus are found as multiple dsRNA fragments. In general, the mycovirus are cryptic (latent) concerning to the effects caused into phenotype of the host fungus, but they can affect the biology of their host by provoking morphological changes, hyper or hypovirulence. Because they are associated to the hypovirulence phenomenon, the mycoviruses show a potential use in the biocontroll of the phytopathogenic fungus. The general objective of this work was to characterize the mycovirus in the isolates of P. griseola, since they were recently detected in this fungus species for the first time. To reach this objective, the following were performed: the characterization of the dsRNA in different isolates; the vertical transmission analysis; and the obtainment of isogenic lines by the virus cure. The dsRNAs were detected in 31 from those 49 isolates of P. griseola under analysis. In the present study, most isolates showed multiple dsRNA fragments varying from zero to 10, as being the sizes estimated between 0.8 and 4.8 kb. The dsRNA fragment of 4.8 kb from the isolate was efficiently transmitted to the asexual spores. However, not all dsRNA fragments (between 1 and 6) found in the isolate Ig848 were transmitted to monosporic colonies. The cycloheximide was used at concentration of 20 µg/mL in order to obtain the mycovirus cure. This treatment was ineffective for the isolate 29-3, since those three colonies transplanted to cyclohexymide during four generations showed the same profile as the total nucleic acids found in the wild isolate. In the case of the isolate Ig848, this same chemical treatment eliminated the fragments 2.2; 2.0; 1.8; 1.2 and 1.0 kb of the colonies Ch2 and Ch4 after seven successive transplantings in medium containing cycloheximide. Several phytopathogenic fungus are attacked by viral infections, and this variation in the profile of the nucleic acid found in the P. griseola isolates is also observed in other plant pathogens. The presence of multiple fragments in only one isolate may be due to the infection by virus with segmented genome, RNA satellite, defective RNA or mixed infections. The efficiency of the transmission by conidia is variable, as depending on the fungus species under consideration, but it is usually near 100% as occurred for the isolate 29-3 of P. griseola. Either cure of some dsRNA fragments and the spontaneous loss during conidiogenesis observed for the isolate Ig848 rather indicate the infection by independent replicons. In those isogenic lines with and without some dsRNA fragments, the effect of the viral infection will be evaluated under some aspects such as the sporulation rate, growth and pathogenicity. The characterization of those viruses found in P. griseola will allow for further studies concerning to their use in the biological control of the angular leaf spot, which will turn possible to reduce the economical losses caused by this disease in agriculture.O feijoeiro comum Phaseolus vulgaris apresenta grande importância alimentar e econômica para o brasileiro. No entanto, sua produtividade é baixa devido, em parte, à ocorrência de doenças. Entre essas doenças, destaca-se a mancha-angular, cujo agente causal é o fungo Pseudocercospora griseola (Sacc.) Crous & U. Braun. Micovírus ou partículas semelhantes a vírus já foram descritas em diversos fungos fitopatogênicos. Esses vírus são incapazes de penetrar e lisar as células hospedeiras, sendo a transmissão intracitoplasmática por meio da anastomose entre hifas e da esporogênese. A maior parte dos micovírus é encontrada como múltiplos fragmentos de dsRNA. Em geral, os micovírus são crípticos (latentes) em relação aos efeitos provocados no fenótipo do fungo hospedeiro, mas podem influenciar a biologia de seu hospedeiro, provocando alterações morfológicas, hiper ou hipovirulência. Por estarem associados ao fenômeno de hipovirulência, os micovírus apresentam uso potencial no biocontrole de fungos fitopatogênicos. O objetivo geral deste trabalho foi caracterizar micovírus presentes em isolados de P. griseola, uma vez que recentemente estes foram detectados, pela primeira vez, nesta espécie de fungo. Para atingir este objetivo, foi realizada a caracterização de dsRNAs presentes em diferentes isolados, a análise da transmissão vertical e a obtenção de linhagens isogênicas por meio da cura de vírus. dsRNAs foram detectados em 31 dos 49 isolados de P. griseola analisados. Neste trabalho, a maioria dos isolados apresentou múltiplos fragmentos de dsRNA, que variaram de zero a 10, com tamanhos estimados entre 0,8 e 4,8 kb. O fragmento de dsRNA de 4,8 kb do isolado 29-3 foi eficientemente transmitido para os esporos assexuais. Entretanto, nem todos os fragmentos de dsRNA, entre um e seis, presentes no isolado Ig848 foram transmitidos para colônias monospóricas. Cicloheximida foi utilizada em concentração de 20 μg/mL a fim de obter a cura de micovírus. Para o isolado 29-3, este tratamento foi ineficaz, pois as três colônias repicadas em cicloheximida durante quatro gerações apresentaram o mesmo perfil de ácidos nucléicos totais presente no isolado selvagem. No caso do isolado Ig848, este mesmo tratamento eliminou os fragmentos de 2,2; 2,0; 1,8; 1,2 e 1,0 kb das colônias Ch2 e Ch4, após sete repicagens sucessivas em meio contendo cicloheximida. Diversos fungos fitopatogênicos são acometidos por infecções virais, sendo que essa variação no perfil de ácidos nucléicos presente nos isolados de P. griseola também é observada em outros patógenos de plantas. A presença de múltiplos fragmentos, em um único isolado, pode ser devido à infecção por vírus com genoma segmentado, RNA satélite, RNA defectivo ou infecções mistas. A eficiência de transmissão por meio dos conídios é variável, dependendo da espécie de fungo considerada, mas geralmente é próxima a 100%, conforme ocorreu para o isolado 29-3 de P. griseola. Tanto a cura de alguns fragmentos de dsRNA quanto a perda espontânea durante a conidiogênese, observada para o isolado Ig848, indicam a infecção por replicons independentes. Estas linhagens isogênicas, com e sem alguns fragmentos de dsRNA, terão o efeito da infecção viral avaliados em aspectos como a taxa de esporulação, o crescimento e a patogenicidade. A caracterização destes vírus, presentes em P. griseola, permitirá estudos posteriores sobre o uso destes no controle biológico da mancha-angular, o que poderá reduzir as perdas econômicas causadas por essa doença na lavoura.Conselho Nacional de Desenvolvimento Científico e Tecnológicoapplication/pdfporUniversidade Federal de ViçosaMestrado em Microbiologia AgrícolaUFVBRAssociações micorrízicas; Bactérias láticas e probióticos; Biologia molecular de fungos de interesseMicovírusPseudocercospora griseolaPhaeoisariopsis griseolaMycovirusPseudocercospora griseolaPhaeoisariopsis griseolaCNPQ::CIENCIAS BIOLOGICAS::GENETICA::GENETICA MOLECULAR E DE MICROORGANISMOSIncidência e transmissão de dsRNA em Pseudocercospora griseola, agente causal da mancha-angular do feijoeiro comum (Phaseolus vulgaris)Incidence and transmission of dsRNA in Phaeoisariopsis griseola, the agent causing the angular leaf spot in the common bean (Phaseolus vulgaris)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINAL01 - capa_abstract.pdfapplication/pdf74907https://locus.ufv.br//bitstream/123456789/5300/1/01%20-%20capa_abstract.pdf25176f30d42e87ea51768c28726ca1bdMD51TEXT01 - capa_abstract.pdf.txt01 - capa_abstract.pdf.txtExtracted texttext/plain8469https://locus.ufv.br//bitstream/123456789/5300/2/01%20-%20capa_abstract.pdf.txt3eaa7e7ba575590a391b32168607489fMD52THUMBNAIL01 - capa_abstract.pdf.jpg01 - capa_abstract.pdf.jpgIM Thumbnailimage/jpeg3644https://locus.ufv.br//bitstream/123456789/5300/3/01%20-%20capa_abstract.pdf.jpgf73220513659f8269b0ad4cc73a40405MD53123456789/53002016-04-10 23:17:06.912oai:locus.ufv.br:123456789/5300Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-11T02:17:06LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.por.fl_str_mv Incidência e transmissão de dsRNA em Pseudocercospora griseola, agente causal da mancha-angular do feijoeiro comum (Phaseolus vulgaris)
dc.title.alternative.eng.fl_str_mv Incidence and transmission of dsRNA in Phaeoisariopsis griseola, the agent causing the angular leaf spot in the common bean (Phaseolus vulgaris)
title Incidência e transmissão de dsRNA em Pseudocercospora griseola, agente causal da mancha-angular do feijoeiro comum (Phaseolus vulgaris)
spellingShingle Incidência e transmissão de dsRNA em Pseudocercospora griseola, agente causal da mancha-angular do feijoeiro comum (Phaseolus vulgaris)
Lima, Swiany Silveira
Micovírus
Pseudocercospora griseola
Phaeoisariopsis griseola
Mycovirus
Pseudocercospora griseola
Phaeoisariopsis griseola
CNPQ::CIENCIAS BIOLOGICAS::GENETICA::GENETICA MOLECULAR E DE MICROORGANISMOS
title_short Incidência e transmissão de dsRNA em Pseudocercospora griseola, agente causal da mancha-angular do feijoeiro comum (Phaseolus vulgaris)
title_full Incidência e transmissão de dsRNA em Pseudocercospora griseola, agente causal da mancha-angular do feijoeiro comum (Phaseolus vulgaris)
title_fullStr Incidência e transmissão de dsRNA em Pseudocercospora griseola, agente causal da mancha-angular do feijoeiro comum (Phaseolus vulgaris)
title_full_unstemmed Incidência e transmissão de dsRNA em Pseudocercospora griseola, agente causal da mancha-angular do feijoeiro comum (Phaseolus vulgaris)
title_sort Incidência e transmissão de dsRNA em Pseudocercospora griseola, agente causal da mancha-angular do feijoeiro comum (Phaseolus vulgaris)
author Lima, Swiany Silveira
author_facet Lima, Swiany Silveira
author_role author
dc.contributor.authorLattes.por.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4190755A5
dc.contributor.author.fl_str_mv Lima, Swiany Silveira
dc.contributor.advisor-co1.fl_str_mv Araujo, Elza Fernandes de
dc.contributor.advisor-co1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783675E2
dc.contributor.advisor-co2.fl_str_mv Zerbini Júnior, Francisco Murilo
dc.contributor.advisor-co2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783743U5
dc.contributor.advisor1.fl_str_mv Queiroz, Marisa Vieira de
dc.contributor.advisor1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785812Z5
dc.contributor.referee1.fl_str_mv Kasuya, Maria Catarina Megumi
dc.contributor.referee1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4721444T5
dc.contributor.referee2.fl_str_mv Barros, Everaldo Gonçalves de
dc.contributor.referee2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781285J6
dc.contributor.referee3.fl_str_mv Costa, Maurício Dutra
dc.contributor.referee3Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4728228J5
contributor_str_mv Araujo, Elza Fernandes de
Zerbini Júnior, Francisco Murilo
Queiroz, Marisa Vieira de
Kasuya, Maria Catarina Megumi
Barros, Everaldo Gonçalves de
Costa, Maurício Dutra
dc.subject.por.fl_str_mv Micovírus
Pseudocercospora griseola
Phaeoisariopsis griseola
topic Micovírus
Pseudocercospora griseola
Phaeoisariopsis griseola
Mycovirus
Pseudocercospora griseola
Phaeoisariopsis griseola
CNPQ::CIENCIAS BIOLOGICAS::GENETICA::GENETICA MOLECULAR E DE MICROORGANISMOS
dc.subject.eng.fl_str_mv Mycovirus
Pseudocercospora griseola
Phaeoisariopsis griseola
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS BIOLOGICAS::GENETICA::GENETICA MOLECULAR E DE MICROORGANISMOS
description The common bean Phaseolus vulgaris shows great importance under feeding and economical aspects for the Brazilian people. However, its productivity has been low due to the occurrence of diseases and other factors. The angular leaf spot is distinguished among those diseases. Its causal agent is the Pseudocercospora griseola (Sacc.) Crous & U. Braun. Some mycovirus or virus-like particles were already described in several phytopathogenic fungus. Those viruses are unable to penetrating and lysing the host cells, and the intracytoplasmic transmission is accomplished by anastomosis among hyphae and the sporogenesis. Most mycovirus are found as multiple dsRNA fragments. In general, the mycovirus are cryptic (latent) concerning to the effects caused into phenotype of the host fungus, but they can affect the biology of their host by provoking morphological changes, hyper or hypovirulence. Because they are associated to the hypovirulence phenomenon, the mycoviruses show a potential use in the biocontroll of the phytopathogenic fungus. The general objective of this work was to characterize the mycovirus in the isolates of P. griseola, since they were recently detected in this fungus species for the first time. To reach this objective, the following were performed: the characterization of the dsRNA in different isolates; the vertical transmission analysis; and the obtainment of isogenic lines by the virus cure. The dsRNAs were detected in 31 from those 49 isolates of P. griseola under analysis. In the present study, most isolates showed multiple dsRNA fragments varying from zero to 10, as being the sizes estimated between 0.8 and 4.8 kb. The dsRNA fragment of 4.8 kb from the isolate was efficiently transmitted to the asexual spores. However, not all dsRNA fragments (between 1 and 6) found in the isolate Ig848 were transmitted to monosporic colonies. The cycloheximide was used at concentration of 20 µg/mL in order to obtain the mycovirus cure. This treatment was ineffective for the isolate 29-3, since those three colonies transplanted to cyclohexymide during four generations showed the same profile as the total nucleic acids found in the wild isolate. In the case of the isolate Ig848, this same chemical treatment eliminated the fragments 2.2; 2.0; 1.8; 1.2 and 1.0 kb of the colonies Ch2 and Ch4 after seven successive transplantings in medium containing cycloheximide. Several phytopathogenic fungus are attacked by viral infections, and this variation in the profile of the nucleic acid found in the P. griseola isolates is also observed in other plant pathogens. The presence of multiple fragments in only one isolate may be due to the infection by virus with segmented genome, RNA satellite, defective RNA or mixed infections. The efficiency of the transmission by conidia is variable, as depending on the fungus species under consideration, but it is usually near 100% as occurred for the isolate 29-3 of P. griseola. Either cure of some dsRNA fragments and the spontaneous loss during conidiogenesis observed for the isolate Ig848 rather indicate the infection by independent replicons. In those isogenic lines with and without some dsRNA fragments, the effect of the viral infection will be evaluated under some aspects such as the sporulation rate, growth and pathogenicity. The characterization of those viruses found in P. griseola will allow for further studies concerning to their use in the biological control of the angular leaf spot, which will turn possible to reduce the economical losses caused by this disease in agriculture.
publishDate 2008
dc.date.issued.fl_str_mv 2008-07-29
dc.date.available.fl_str_mv 2009-02-12
2015-03-26T13:51:44Z
dc.date.accessioned.fl_str_mv 2015-03-26T13:51:44Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
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dc.identifier.citation.fl_str_mv LIMA, Swiany Silveira. Incidence and transmission of dsRNA in Phaeoisariopsis griseola, the agent causing the angular leaf spot in the common bean (Phaseolus vulgaris). 2008. 5 f. Dissertação (Mestrado em Associações micorrízicas; Bactérias láticas e probióticos; Biologia molecular de fungos de interesse) - Universidade Federal de Viçosa, Viçosa, 2008.
dc.identifier.uri.fl_str_mv http://locus.ufv.br/handle/123456789/5300
identifier_str_mv LIMA, Swiany Silveira. Incidence and transmission of dsRNA in Phaeoisariopsis griseola, the agent causing the angular leaf spot in the common bean (Phaseolus vulgaris). 2008. 5 f. Dissertação (Mestrado em Associações micorrízicas; Bactérias láticas e probióticos; Biologia molecular de fungos de interesse) - Universidade Federal de Viçosa, Viçosa, 2008.
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dc.publisher.country.fl_str_mv BR
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publisher.none.fl_str_mv Universidade Federal de Viçosa
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