Mycovirus in Pseudocercospora griseola, the causal agent of angular leaf spot in common bean

Detalhes bibliográficos
Autor(a) principal: Lima, Swiany Silveira
Data de Publicação: 2010
Outros Autores: Abadio, Ana Karina Rodrigues, Araújo, Elza Fernandes, Kitajima, Elliot Watanabe, Watanabe, Elliot, Queiroz, Marisa Vieira de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://dx.doi.org/10.1139/W10-022
http://www.locus.ufv.br/handle/123456789/23333
Resumo: Pseudocercospora griseola (Sacc.) Crous & Braun is a widespread fungal phytopathogen that is responsible for angular leaf spot in the common bean (Phaseolus vulgaris L.). A number of fungal phytopathogens have been shown to harbour mycoviruses, and this possibility was investigated in populations of Pseudocercospora griseola. The total nucleic acid extracts of 61 fungal isolates were subjected to agarose gel electrophoresis. Small fragments (800–4800 bp) could be identified in 42 of the samples. The presence of dsRNA in isolate Ig838 was confirmed by treatment of total nucleic acid with DNase, RNase A, and nuclease S1. Transmission electron microscopy revealed the presence of viral-like particles 40 nm in diameter in the mycelia of 2 fungal isolates, namely 29-3 and Ig838. The transmission of dsRNA by means of conidia was 100% for isolate 29-3, but there was loss of 1–6 fragments of dsRNA in monosporic colonies of isolate Ig848. Cycloheximide treatment failed to inhibit the mycovirus in isolate 29-3, but proved efficient in the elimination of the 2.2, 2.0, 1.8, 1.2 and 1.0 kb fragments in 2 colonies of isolate Ig848. The occurrence of a mycovirus in Pseudocercospora griseola was demonstrated for the first time in the present study.
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spelling Lima, Swiany SilveiraAbadio, Ana Karina RodriguesAraújo, Elza FernandesKitajima, Elliot WatanabeWatanabe, ElliotQueiroz, Marisa Vieira de2019-02-04T17:48:57Z2019-02-04T17:48:57Z201014803275http://dx.doi.org/10.1139/W10-022http://www.locus.ufv.br/handle/123456789/23333Pseudocercospora griseola (Sacc.) Crous & Braun is a widespread fungal phytopathogen that is responsible for angular leaf spot in the common bean (Phaseolus vulgaris L.). A number of fungal phytopathogens have been shown to harbour mycoviruses, and this possibility was investigated in populations of Pseudocercospora griseola. The total nucleic acid extracts of 61 fungal isolates were subjected to agarose gel electrophoresis. Small fragments (800–4800 bp) could be identified in 42 of the samples. The presence of dsRNA in isolate Ig838 was confirmed by treatment of total nucleic acid with DNase, RNase A, and nuclease S1. Transmission electron microscopy revealed the presence of viral-like particles 40 nm in diameter in the mycelia of 2 fungal isolates, namely 29-3 and Ig838. The transmission of dsRNA by means of conidia was 100% for isolate 29-3, but there was loss of 1–6 fragments of dsRNA in monosporic colonies of isolate Ig848. Cycloheximide treatment failed to inhibit the mycovirus in isolate 29-3, but proved efficient in the elimination of the 2.2, 2.0, 1.8, 1.2 and 1.0 kb fragments in 2 colonies of isolate Ig848. The occurrence of a mycovirus in Pseudocercospora griseola was demonstrated for the first time in the present study.Pseudocercospora griseola (Sacc.) Crous & Braun est un phytopathogène fongique répandu qui cause la maladie des taches anguleuses du haricot (Phaseolus vulgaris L.). Puisqu’un certain nombre de phytopathogènes fongiques sont porteurs de mycovirus, cette possibilité a été examinée chez Pseudocercospora griseola. Des extraits d’acides nucléiques totaux de 61 isolats fongiques ont été soumis à une électrophorèse sur agarose. Des petits fragments (800–4800 pb) ont puêtre identifiés chez 42 des isolats. La présence d’ARN db dans l’isolat Ig838 a été confirmée par un traitement des acides nucléiques totaux à la DNase, à la RNase A et à la nucléase S1. La microscopie électronique à transmission a révélé la présence de particules virales de 40 nm de diamètre dans le mycélium de 2 isolats fongiques, soit 29-3 et Ig838. La transmission de l’ARN db par l’intermédiaire de conidies était de 100 % chez 29-3, mais de 1–6 fragments d’ARN db étaient perdus dans les colonies monosporiques de Ig848. Un traitement à la cycloheximide n’a pu inhiber le mycovirus dans l’isolat 29-3, mais s’est montré efficace pour éliminer les fragments de 2,2, 2,0, 1,8, 1,2 et 1,0 kb dans 2 colonies de l’isolat Ig848. La présence d’un mycovirus chez Pseudocercospora griseola a été ici démontrée pour la première fois.engCanadian Journal of Microbiologyv. 56, n. 5, p. 359– 365, mai. 2010NRC Research Pressinfo:eu-repo/semantics/openAccessdsRNAMycovirusPseudocercospora griseolaPhaeoisariopsis griseolaMycovirus in Pseudocercospora griseola, the causal agent of angular leaf spot in common beaninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdftexto completoapplication/pdf724607https://locus.ufv.br//bitstream/123456789/23333/1/artigo.pdfef187512a8c9f9b1898332660129ab32MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/23333/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52123456789/233332019-02-04 14:52:50.428oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452019-02-04T17:52:50LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.en.fl_str_mv Mycovirus in Pseudocercospora griseola, the causal agent of angular leaf spot in common bean
title Mycovirus in Pseudocercospora griseola, the causal agent of angular leaf spot in common bean
spellingShingle Mycovirus in Pseudocercospora griseola, the causal agent of angular leaf spot in common bean
Lima, Swiany Silveira
dsRNA
Mycovirus
Pseudocercospora griseola
Phaeoisariopsis griseola
title_short Mycovirus in Pseudocercospora griseola, the causal agent of angular leaf spot in common bean
title_full Mycovirus in Pseudocercospora griseola, the causal agent of angular leaf spot in common bean
title_fullStr Mycovirus in Pseudocercospora griseola, the causal agent of angular leaf spot in common bean
title_full_unstemmed Mycovirus in Pseudocercospora griseola, the causal agent of angular leaf spot in common bean
title_sort Mycovirus in Pseudocercospora griseola, the causal agent of angular leaf spot in common bean
author Lima, Swiany Silveira
author_facet Lima, Swiany Silveira
Abadio, Ana Karina Rodrigues
Araújo, Elza Fernandes
Kitajima, Elliot Watanabe
Watanabe, Elliot
Queiroz, Marisa Vieira de
author_role author
author2 Abadio, Ana Karina Rodrigues
Araújo, Elza Fernandes
Kitajima, Elliot Watanabe
Watanabe, Elliot
Queiroz, Marisa Vieira de
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Lima, Swiany Silveira
Abadio, Ana Karina Rodrigues
Araújo, Elza Fernandes
Kitajima, Elliot Watanabe
Watanabe, Elliot
Queiroz, Marisa Vieira de
dc.subject.pt-BR.fl_str_mv dsRNA
Mycovirus
Pseudocercospora griseola
Phaeoisariopsis griseola
topic dsRNA
Mycovirus
Pseudocercospora griseola
Phaeoisariopsis griseola
description Pseudocercospora griseola (Sacc.) Crous & Braun is a widespread fungal phytopathogen that is responsible for angular leaf spot in the common bean (Phaseolus vulgaris L.). A number of fungal phytopathogens have been shown to harbour mycoviruses, and this possibility was investigated in populations of Pseudocercospora griseola. The total nucleic acid extracts of 61 fungal isolates were subjected to agarose gel electrophoresis. Small fragments (800–4800 bp) could be identified in 42 of the samples. The presence of dsRNA in isolate Ig838 was confirmed by treatment of total nucleic acid with DNase, RNase A, and nuclease S1. Transmission electron microscopy revealed the presence of viral-like particles 40 nm in diameter in the mycelia of 2 fungal isolates, namely 29-3 and Ig838. The transmission of dsRNA by means of conidia was 100% for isolate 29-3, but there was loss of 1–6 fragments of dsRNA in monosporic colonies of isolate Ig848. Cycloheximide treatment failed to inhibit the mycovirus in isolate 29-3, but proved efficient in the elimination of the 2.2, 2.0, 1.8, 1.2 and 1.0 kb fragments in 2 colonies of isolate Ig848. The occurrence of a mycovirus in Pseudocercospora griseola was demonstrated for the first time in the present study.
publishDate 2010
dc.date.issued.fl_str_mv 2010
dc.date.accessioned.fl_str_mv 2019-02-04T17:48:57Z
dc.date.available.fl_str_mv 2019-02-04T17:48:57Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1139/W10-022
http://www.locus.ufv.br/handle/123456789/23333
dc.identifier.issn.none.fl_str_mv 14803275
identifier_str_mv 14803275
url http://dx.doi.org/10.1139/W10-022
http://www.locus.ufv.br/handle/123456789/23333
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartofseries.pt-BR.fl_str_mv v. 56, n. 5, p. 359– 365, mai. 2010
dc.rights.driver.fl_str_mv NRC Research Press
info:eu-repo/semantics/openAccess
rights_invalid_str_mv NRC Research Press
eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv Canadian Journal of Microbiology
publisher.none.fl_str_mv Canadian Journal of Microbiology
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