Utilização de microesferas biodegradáveis PLGA como sistema de liberação para a vacina sintética SBm7462 no controle do Boophilus microplus (Canestrini, 1887): modelo experimental em camundongos

• Autor(a) principal: Sales Junior, Policarpo Ademar
• Data de Publicação: 2003
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/5172
• topic: Boophilus microplus; Vacina sintética; Adjuvantes; Boophilus microplus; Synthetic vaccine; Adjuvants; CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::MEDICINA VETERINARIA PREVENTIVA

Was evaluated the immunological response of synthetic peptide SBm7462 emulsified with the adjuvant saponin or encapsulated in biodegradable PLGA microspheres; as well as, the response of synthetic peptide SPf66 encapsulated on cited delivery system. Mice Balb/c, female, were utilized on study, with a dose of 100mg of peptide for each animal. The mice were separated in six groups of twenty: group I: animals inoculated with 75mg of saponin; group II: inoculated with 100mL of water milliQ; group III: immunized with the peptide SBm7462 emulsified on saponin, 75mg of saponin for 100mg of peptide; group IV: inoculated with empty microspheres; group V: immunized with the peptide SBm7462 enclausured in PLGA microspheres; group VI: immunized with the synthetic peptide SPf66 enclausured in PLGA microspheres. The three inoculations was executed on day zero and latter on third and sixth weeks after the first inoculation. The mice were bled prior of first immunisation and at periodic intervals (of seven days) until week 21. The serum was assayed by indirect ELISA for detection of specific IgGs anti-SBm7462, and for realization of tests of hepatic function. The synthetic peptide SBm7462 showed a better immune response emulsified with saponin adjuvant. The peptide SPf66 showed a similar response when compared with the obtained for others authors when encapsulated in PLGA microspheres and utilized in mice. The tests of hepatic function were realized in equipament multiparametric of bioquimic Alisé, and no evidence of hepatotoxic effects was viewed, when was evaluated the parameters: total proteins, albumin, AST and ALT. In order to verification of histologic alterations post-immunization, were colleted spleen of mice, for each treatment, on day zero, three, six and nine days post the second inoculation and two, four and six days post the third inoculation. The results obtained showed the saponin potentiate a better immune response of peptide SBm7462, and this response was more precoce than the obtained with the PLGA microspheres. This delivery system show itself as a good approach for utilization with the synthetic peptide SBm7462, nevertheless more studies are necessary for promotion of a better efficacy of encapsulation.