Avaliação dos peptídeos sintéticos SBbo23290 e SBm7462 na forma monovalente e polivalente em bovinos desafiados com Riphicephalus (Boophilus) microplus (CANESTRINI, 1887) e Babesia bovis (BABES, 1888; STARCOVICI, 1888)

• Autor(a) principal: Montano, Javier Antonio Benavides
• Data de Publicação: 2006
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/5079
• topic: Babesia bovis; Riphicephalus boophilus; Boophilus microplus; Resposta imune-humoral; Babesia bovis; Riphicephalus boophilus; Boophilus microplus; Immunology response; CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::MEDICINA VETERINARIA PREVENTIVA

It was evaluated the immunology response given by the SBbo23290 and SBm7462 synthetic peptides to the control of Babesia bovis and Riphicephalus (Boophilus) microplus, inoculated by subcutaneous route, individually and simultaneously (Group I) and in a cocktail of both peptides (Group II). Each inoculation was done with intervals of 30 days. In the 30th and 34th days after the 3th inoculation, it was applied in each animal, 4000 larvaes of R. (B.) microplus, free of blood parasites and also, it was inoculated a sample of Babesia bovis UFV1 9th passage with a concentration of 1x106 babesias/mL, respectively. According to the results, the animals inoculated with the Group II obtained greater clinic response after challenging with both parasites. Besides, these animals showed high levels of total IgG specific to the SBbo23290, beginning one week after 1st inoculation, with titles of 1,06 ± 0,2486. The titles with high affinity of total IgG to SBm7462 was presented from the 8th week, with levels of 1,75 ± 0,1849 and 1,13 ± 0,2819 to Groups I and II, respectively. Both Group showed high levels of IgG1 over IgG2 isotopes with high affinity to SBbo23290. The Group I showed titles of total IgG and IgG1 with high affinity to SBm7462 and low efficiency (EF) to control the ticks, different of the obtained in the Group II, where it was found high titles of total IgG, low levels of IgG1 and EF of 46,80 %. These results suggest that, intraclonal competition was present after presentation by APCs (DC or WC1.1+) to CD4+ and lymphocytes B in the germinal centers, with major affinity by specific clones to SBbo23290 over SBm7462. It is possible that these changes were determined by antigen solubility.