Avaliação de Listeria monocytogenes em melão e jabuticaba

Detalhes bibliográficos
Autor(a) principal: Souza, Poliana Bergamin Athayde de
Data de Publicação: 2014
Tipo de documento: Dissertação
Idioma: por
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://locus.ufv.br/handle/123456789/2949
Resumo: Consumer demand for fresh foods has grown substantially in recent decades. However, despite these products preserve better sensory and nutritional characteristics, contamination by pathogens, such as L. monocytogenes, generates increasing concerns.Infection by this bacteria, called listeriosis, has low morbidity but high mortality, and represents a high risk to immunocompromised individuals, pregnant women, elderly and children. Fresh products are considered high risk, since it does not go through steps to ensure the elimination of pathogens, in addition, are normally stored under refrigeration condition in which L. monocytogenes can multiply by providing conditions for growth protrudes in comparison to most bacteria. Given the above, the occurrence of L. monocytogenes was investigated in two fruits commonly consumed in natura: melon and jabuticaba. A total of 150 samples of melon and 60 samples jabuticaba acquired at retail in the municipality of Viçosa-MG, were subjected to laboratory analysis. Through conventional analysis using the method ISO 11290-1, 21 isolates of Listeria spp. were found in the fruits of melon, of which only 14 were confirmed by sequencing the gene. The presence of L. monocytogenes was also evaluated by the mini-VIDAS® LMO and by PCR. A strain of L. monocytogenes was found by PCR, however, this result differed from that obtained by imunoanalítico test, which indicated absence of L. monocytogenes and showed a rate of 3.12% false negative results. Serotyping of strains was performed by the Instituto Oswaldo Cruz (Fiocruz) and the serotyp identified was 1/2b one of the most associated with cases of human listeriosis in the world, suggesting the potential risk of to cause disease. The results evidence that the melon is a potential source of L. monocytogenes and that measures to control and prevent this pathogen should be adopted with greater persistence for products that are usually eaten fresh as fruit.
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spelling Souza, Poliana Bergamin Athayde dehttp://lattes.cnpq.br/9098881511660932Vanetti, Maria Cristina Dantashttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783874H3Peña, Wilmer Edgard Luerahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4730660Z8Andrade, Nélio José dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4788281Y5Antunes, Maria Aparecidahttp://lattes.cnpq.br/35265930058869902015-03-26T13:13:31Z2015-01-212015-03-26T13:13:31Z2014-08-15SOUZA, Poliana Bergamin Athayde de. Evaluation of Listeria monocytogenes in melon and jabuticaba. 2014. 72 f. Dissertação (Mestrado em Ciência de Alimentos; Tecnologia de Alimentos; Engenharia de Alimentos) - Universidade Federal de Viçosa, Viçosa, 2014.http://locus.ufv.br/handle/123456789/2949Consumer demand for fresh foods has grown substantially in recent decades. However, despite these products preserve better sensory and nutritional characteristics, contamination by pathogens, such as L. monocytogenes, generates increasing concerns.Infection by this bacteria, called listeriosis, has low morbidity but high mortality, and represents a high risk to immunocompromised individuals, pregnant women, elderly and children. Fresh products are considered high risk, since it does not go through steps to ensure the elimination of pathogens, in addition, are normally stored under refrigeration condition in which L. monocytogenes can multiply by providing conditions for growth protrudes in comparison to most bacteria. Given the above, the occurrence of L. monocytogenes was investigated in two fruits commonly consumed in natura: melon and jabuticaba. A total of 150 samples of melon and 60 samples jabuticaba acquired at retail in the municipality of Viçosa-MG, were subjected to laboratory analysis. Through conventional analysis using the method ISO 11290-1, 21 isolates of Listeria spp. were found in the fruits of melon, of which only 14 were confirmed by sequencing the gene. The presence of L. monocytogenes was also evaluated by the mini-VIDAS® LMO and by PCR. A strain of L. monocytogenes was found by PCR, however, this result differed from that obtained by imunoanalítico test, which indicated absence of L. monocytogenes and showed a rate of 3.12% false negative results. Serotyping of strains was performed by the Instituto Oswaldo Cruz (Fiocruz) and the serotyp identified was 1/2b one of the most associated with cases of human listeriosis in the world, suggesting the potential risk of to cause disease. The results evidence that the melon is a potential source of L. monocytogenes and that measures to control and prevent this pathogen should be adopted with greater persistence for products that are usually eaten fresh as fruit.A demanda dos consumidores por alimentos frescos tem crescido substancialmente nas ultimas décadas. No entanto, apesar desses produtos preservarem melhor as características sensoriais e nutricionais, a contaminação por patógenos, como L. monocytogenes, gera preocupações crescentes. A doença infecciosa causada por esta bactéria, denominada listeriose, apresenta baixa taxa de morbidade, porém alta de mortalidade, e representa grande risco para indivíduos imunodeprimidos, gestantes, idosos e recém-nascidos. Produtos consumidos frescos são considerados de grande importância, uma vez que não passam por etapas que garantam a eliminação de patógenos, além disso, normalmente são mantidos sob refrigeração, condição na qual L. monocytogenes se multiplica, possibilitando que seu crescimento se sobressaia em relação à maioria das bactérias. Diante do exposto, a ocorrência de L. monocytogenes foi pesquisada em duas frutas que normalmente são consumidas in natura: melão e jabuticaba. O total de 150 amostras de melão e 60 de jabuticaba, adquiridas no comércio varejista do Município de Viçosa-MG, foram submetidas a análises laboratoriais. Por meio de análise convencional, utilizando a metodologia ISO 11290-1, 21 isolados de Listeria spp. foram encontrados nos frutos de melão, dos quais, somente 14 foram confirmados, por meio de sequenciamento gênico. A presença de L. monocytogenes foi avaliada por análise imunoanalítica através do kit VIDAS®- LMO e por técnica de PCR. Uma estirpe de L. monocytogenesfoi encontrada pela reação de PCR, no entanto, este resultado diferiu daquele obtido pelo teste imunoanalítico,o qual indicou ausência de L.monocytogenese apresentou uma taxa de 3,12% de resultados falsos-negativos. A sorotipagem da cepa foi realizada pelo Instituto Oswaldo Cruz (Fiocruz) e o sorotipo idenficado foi o 1/2b, um dos mais associados à casos de listeriose humana no mundo, o que sugere o potencial da cepa em provocar a doença.Os resultados obtidos evidenciam que o melão é uma potencial fonte deL. monocytogenes e que medidas de controle e prevenção deste patógeno devem ser adotadas com maior severidade para produtos que normalmente são consumidos frescos, como as frutas.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de ViçosaMestrado em Ciência e Tecnologia de AlimentosUFVBRCiência de Alimentos; Tecnologia de Alimentos; Engenharia de AlimentosAlimentos frescosListerioseFrutasFresh foodListeriosisFruitsCNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::CIENCIA DE ALIMENTOSAvaliação de Listeria monocytogenes em melão e jabuticabaEvaluation of Listeria monocytogenes in melon and jabuticabainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf544915https://locus.ufv.br//bitstream/123456789/2949/1/texto%20completo.pdfbbc73d70df7c8319393e9ccde68612e2MD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain120754https://locus.ufv.br//bitstream/123456789/2949/2/texto%20completo.pdf.txtb6b212b624517f77e7b1e85dcd5bb079MD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3581https://locus.ufv.br//bitstream/123456789/2949/3/texto%20completo.pdf.jpg1f35fb77d9a9b24d9112510350da51faMD53123456789/29492016-04-08 23:19:52.445oai:locus.ufv.br:123456789/2949Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-09T02:19:52LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.por.fl_str_mv Avaliação de Listeria monocytogenes em melão e jabuticaba
dc.title.alternative.eng.fl_str_mv Evaluation of Listeria monocytogenes in melon and jabuticaba
title Avaliação de Listeria monocytogenes em melão e jabuticaba
spellingShingle Avaliação de Listeria monocytogenes em melão e jabuticaba
Souza, Poliana Bergamin Athayde de
Alimentos frescos
Listeriose
Frutas
Fresh food
Listeriosis
Fruits
CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::CIENCIA DE ALIMENTOS
title_short Avaliação de Listeria monocytogenes em melão e jabuticaba
title_full Avaliação de Listeria monocytogenes em melão e jabuticaba
title_fullStr Avaliação de Listeria monocytogenes em melão e jabuticaba
title_full_unstemmed Avaliação de Listeria monocytogenes em melão e jabuticaba
title_sort Avaliação de Listeria monocytogenes em melão e jabuticaba
author Souza, Poliana Bergamin Athayde de
author_facet Souza, Poliana Bergamin Athayde de
author_role author
dc.contributor.authorLattes.por.fl_str_mv http://lattes.cnpq.br/9098881511660932
dc.contributor.author.fl_str_mv Souza, Poliana Bergamin Athayde de
dc.contributor.advisor-co1.fl_str_mv Vanetti, Maria Cristina Dantas
dc.contributor.advisor-co1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783874H3
dc.contributor.advisor-co2.fl_str_mv Peña, Wilmer Edgard Luera
dc.contributor.advisor-co2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4730660Z8
dc.contributor.advisor1.fl_str_mv Andrade, Nélio José de
dc.contributor.advisor1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4788281Y5
dc.contributor.referee1.fl_str_mv Antunes, Maria Aparecida
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/3526593005886990
contributor_str_mv Vanetti, Maria Cristina Dantas
Peña, Wilmer Edgard Luera
Andrade, Nélio José de
Antunes, Maria Aparecida
dc.subject.por.fl_str_mv Alimentos frescos
Listeriose
Frutas
topic Alimentos frescos
Listeriose
Frutas
Fresh food
Listeriosis
Fruits
CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::CIENCIA DE ALIMENTOS
dc.subject.eng.fl_str_mv Fresh food
Listeriosis
Fruits
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::CIENCIA DE ALIMENTOS
description Consumer demand for fresh foods has grown substantially in recent decades. However, despite these products preserve better sensory and nutritional characteristics, contamination by pathogens, such as L. monocytogenes, generates increasing concerns.Infection by this bacteria, called listeriosis, has low morbidity but high mortality, and represents a high risk to immunocompromised individuals, pregnant women, elderly and children. Fresh products are considered high risk, since it does not go through steps to ensure the elimination of pathogens, in addition, are normally stored under refrigeration condition in which L. monocytogenes can multiply by providing conditions for growth protrudes in comparison to most bacteria. Given the above, the occurrence of L. monocytogenes was investigated in two fruits commonly consumed in natura: melon and jabuticaba. A total of 150 samples of melon and 60 samples jabuticaba acquired at retail in the municipality of Viçosa-MG, were subjected to laboratory analysis. Through conventional analysis using the method ISO 11290-1, 21 isolates of Listeria spp. were found in the fruits of melon, of which only 14 were confirmed by sequencing the gene. The presence of L. monocytogenes was also evaluated by the mini-VIDAS® LMO and by PCR. A strain of L. monocytogenes was found by PCR, however, this result differed from that obtained by imunoanalítico test, which indicated absence of L. monocytogenes and showed a rate of 3.12% false negative results. Serotyping of strains was performed by the Instituto Oswaldo Cruz (Fiocruz) and the serotyp identified was 1/2b one of the most associated with cases of human listeriosis in the world, suggesting the potential risk of to cause disease. The results evidence that the melon is a potential source of L. monocytogenes and that measures to control and prevent this pathogen should be adopted with greater persistence for products that are usually eaten fresh as fruit.
publishDate 2014
dc.date.issued.fl_str_mv 2014-08-15
dc.date.accessioned.fl_str_mv 2015-03-26T13:13:31Z
dc.date.available.fl_str_mv 2015-01-21
2015-03-26T13:13:31Z
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dc.identifier.citation.fl_str_mv SOUZA, Poliana Bergamin Athayde de. Evaluation of Listeria monocytogenes in melon and jabuticaba. 2014. 72 f. Dissertação (Mestrado em Ciência de Alimentos; Tecnologia de Alimentos; Engenharia de Alimentos) - Universidade Federal de Viçosa, Viçosa, 2014.
dc.identifier.uri.fl_str_mv http://locus.ufv.br/handle/123456789/2949
identifier_str_mv SOUZA, Poliana Bergamin Athayde de. Evaluation of Listeria monocytogenes in melon and jabuticaba. 2014. 72 f. Dissertação (Mestrado em Ciência de Alimentos; Tecnologia de Alimentos; Engenharia de Alimentos) - Universidade Federal de Viçosa, Viçosa, 2014.
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