Somatic embryogenesis and de novo shoot organogenesis can be alternatively induced by reactivating pericycle cells in Lisianthus (Eustoma grandiflorum (Raf.) Shinners) root explants
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | http://dx.doi.org/10.1007/s11627-017-9800-2 http://www.locus.ufv.br/handle/123456789/21714 |
Resumo: | This study demonstrated that somatic embryogenesis and de novo shoot organogenesis-based systems of root-derived Lisianthus (Eustoma grandiflorum) explants can be alternatively induced by exogenous supply of auxin or cytokinin. Somatic embryogenesis was observed when root explants were cultured in the dark on Murashige and Skoog-based medium supplemented with 10 μM 2,4-dichlorophenoxyacetic acid (2,4-D). Somatic embryos were differentiated by transferring embryonic calluses to an embryo conversion phase medium containing 2 μM 6-benzyladenine (BA) to promote full plantlet development. Regarding de novo shoot organogenesis, the addition of 4 μM of either BA or zeatin was the most effective treatment for inducing adventitious shoot buds. A detailed histological characterization of somatic embryogenesis and de novo shoot organogenesis showed that both morphogenetic processes shared the same cellular origin. The formation of somatic embryos and adventitious shoot buds occurred through the reactivation of pericycle and vascular parenchyma cells into proembryos and meristemoids, respectively, which consisted of meristematic cells with similar characteristics. These results provide further evidence of optimization of in vitro propagation as a useful approach to improve this important ornamental species. |
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LOCUS Repositório Institucional da UFV |
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Somatic embryogenesis and de novo shoot organogenesis can be alternatively induced by reactivating pericycle cells in Lisianthus (Eustoma grandiflorum (Raf.) Shinners) root explantsIn vitro organogenesisOrnamental plantPericycleRoot cultureSomatic embryogenesisThis study demonstrated that somatic embryogenesis and de novo shoot organogenesis-based systems of root-derived Lisianthus (Eustoma grandiflorum) explants can be alternatively induced by exogenous supply of auxin or cytokinin. Somatic embryogenesis was observed when root explants were cultured in the dark on Murashige and Skoog-based medium supplemented with 10 μM 2,4-dichlorophenoxyacetic acid (2,4-D). Somatic embryos were differentiated by transferring embryonic calluses to an embryo conversion phase medium containing 2 μM 6-benzyladenine (BA) to promote full plantlet development. Regarding de novo shoot organogenesis, the addition of 4 μM of either BA or zeatin was the most effective treatment for inducing adventitious shoot buds. A detailed histological characterization of somatic embryogenesis and de novo shoot organogenesis showed that both morphogenetic processes shared the same cellular origin. The formation of somatic embryos and adventitious shoot buds occurred through the reactivation of pericycle and vascular parenchyma cells into proembryos and meristemoids, respectively, which consisted of meristematic cells with similar characteristics. These results provide further evidence of optimization of in vitro propagation as a useful approach to improve this important ornamental species.In Vitro Cellular & Developmental Biology - Plant2018-09-09T23:02:10Z2018-09-09T23:02:10Z2017-03-24info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlepdfapplication/pdf14752689http://dx.doi.org/10.1007/s11627-017-9800-2http://www.locus.ufv.br/handle/123456789/21714engv. 53, n. 3, p. 209– 218 , jun. 2017Springer Nature Switzerland AG.info:eu-repo/semantics/openAccessYumbla-Orbes, MariaCruz, Ana Claudia Ferreira daPinheiro, Marcos Vinicius MarquesRocha, Diego IsmaelBatista, Diego SilvaKoehler, Andréa DiasBarbosa, José GeraldoOtoni, Wagner Camposreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFV2024-07-12T06:21:19Zoai:locus.ufv.br:123456789/21714Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452024-07-12T06:21:19LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.none.fl_str_mv |
Somatic embryogenesis and de novo shoot organogenesis can be alternatively induced by reactivating pericycle cells in Lisianthus (Eustoma grandiflorum (Raf.) Shinners) root explants |
title |
Somatic embryogenesis and de novo shoot organogenesis can be alternatively induced by reactivating pericycle cells in Lisianthus (Eustoma grandiflorum (Raf.) Shinners) root explants |
spellingShingle |
Somatic embryogenesis and de novo shoot organogenesis can be alternatively induced by reactivating pericycle cells in Lisianthus (Eustoma grandiflorum (Raf.) Shinners) root explants Yumbla-Orbes, Maria In vitro organogenesis Ornamental plant Pericycle Root culture Somatic embryogenesis |
title_short |
Somatic embryogenesis and de novo shoot organogenesis can be alternatively induced by reactivating pericycle cells in Lisianthus (Eustoma grandiflorum (Raf.) Shinners) root explants |
title_full |
Somatic embryogenesis and de novo shoot organogenesis can be alternatively induced by reactivating pericycle cells in Lisianthus (Eustoma grandiflorum (Raf.) Shinners) root explants |
title_fullStr |
Somatic embryogenesis and de novo shoot organogenesis can be alternatively induced by reactivating pericycle cells in Lisianthus (Eustoma grandiflorum (Raf.) Shinners) root explants |
title_full_unstemmed |
Somatic embryogenesis and de novo shoot organogenesis can be alternatively induced by reactivating pericycle cells in Lisianthus (Eustoma grandiflorum (Raf.) Shinners) root explants |
title_sort |
Somatic embryogenesis and de novo shoot organogenesis can be alternatively induced by reactivating pericycle cells in Lisianthus (Eustoma grandiflorum (Raf.) Shinners) root explants |
author |
Yumbla-Orbes, Maria |
author_facet |
Yumbla-Orbes, Maria Cruz, Ana Claudia Ferreira da Pinheiro, Marcos Vinicius Marques Rocha, Diego Ismael Batista, Diego Silva Koehler, Andréa Dias Barbosa, José Geraldo Otoni, Wagner Campos |
author_role |
author |
author2 |
Cruz, Ana Claudia Ferreira da Pinheiro, Marcos Vinicius Marques Rocha, Diego Ismael Batista, Diego Silva Koehler, Andréa Dias Barbosa, José Geraldo Otoni, Wagner Campos |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Yumbla-Orbes, Maria Cruz, Ana Claudia Ferreira da Pinheiro, Marcos Vinicius Marques Rocha, Diego Ismael Batista, Diego Silva Koehler, Andréa Dias Barbosa, José Geraldo Otoni, Wagner Campos |
dc.subject.por.fl_str_mv |
In vitro organogenesis Ornamental plant Pericycle Root culture Somatic embryogenesis |
topic |
In vitro organogenesis Ornamental plant Pericycle Root culture Somatic embryogenesis |
description |
This study demonstrated that somatic embryogenesis and de novo shoot organogenesis-based systems of root-derived Lisianthus (Eustoma grandiflorum) explants can be alternatively induced by exogenous supply of auxin or cytokinin. Somatic embryogenesis was observed when root explants were cultured in the dark on Murashige and Skoog-based medium supplemented with 10 μM 2,4-dichlorophenoxyacetic acid (2,4-D). Somatic embryos were differentiated by transferring embryonic calluses to an embryo conversion phase medium containing 2 μM 6-benzyladenine (BA) to promote full plantlet development. Regarding de novo shoot organogenesis, the addition of 4 μM of either BA or zeatin was the most effective treatment for inducing adventitious shoot buds. A detailed histological characterization of somatic embryogenesis and de novo shoot organogenesis showed that both morphogenetic processes shared the same cellular origin. The formation of somatic embryos and adventitious shoot buds occurred through the reactivation of pericycle and vascular parenchyma cells into proembryos and meristemoids, respectively, which consisted of meristematic cells with similar characteristics. These results provide further evidence of optimization of in vitro propagation as a useful approach to improve this important ornamental species. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-03-24 2018-09-09T23:02:10Z 2018-09-09T23:02:10Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
14752689 http://dx.doi.org/10.1007/s11627-017-9800-2 http://www.locus.ufv.br/handle/123456789/21714 |
identifier_str_mv |
14752689 |
url |
http://dx.doi.org/10.1007/s11627-017-9800-2 http://www.locus.ufv.br/handle/123456789/21714 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
v. 53, n. 3, p. 209– 218 , jun. 2017 |
dc.rights.driver.fl_str_mv |
Springer Nature Switzerland AG. info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Springer Nature Switzerland AG. |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
pdf application/pdf |
dc.publisher.none.fl_str_mv |
In Vitro Cellular & Developmental Biology - Plant |
publisher.none.fl_str_mv |
In Vitro Cellular & Developmental Biology - Plant |
dc.source.none.fl_str_mv |
reponame:LOCUS Repositório Institucional da UFV instname:Universidade Federal de Viçosa (UFV) instacron:UFV |
instname_str |
Universidade Federal de Viçosa (UFV) |
instacron_str |
UFV |
institution |
UFV |
reponame_str |
LOCUS Repositório Institucional da UFV |
collection |
LOCUS Repositório Institucional da UFV |
repository.name.fl_str_mv |
LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV) |
repository.mail.fl_str_mv |
fabiojreis@ufv.br |
_version_ |
1822610547529482240 |