Avaliação, in vivo e in vitro, dos efeitos dos flavonóides quercetina, naringenina e ipriflavona na prevenção e tratamento do tumor ascítico de Ehrlich

• Autor(a) principal: Fonseca, Cristiane Sampaio
• Data de Publicação: 2006
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/289
• topic: Flavonóides; Câncer; Tumores ascíticos; Agentes antineoplásicos; Flavonoids; Cancer; Ascitic tumours; Antineoplastic agents; CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA

Every year appears, in the world, thousands of new cases of cancer in human beings. In Brazil, he/she is considered more than 470.000 new cases for the year of 2006. Studies tell that many natural products are studied as agents quimioprotetors against several cancers of world occurrence. The flavonoids exercise beneficial effects in the prevention of the cancer, due to ability to inhibit the cellular cycle, the cellular proliferation, oxidative stress and to induce detoxification of enzymes and apoptose. The discovery of the transplantation of the tumors favored the study of the behavior of several cancers. The Ehrlich.ascitic tumor (TAE) it was the model used in that study in mice Swiss, inoculated with the tumor cells by road intraperitoneal, to evaluate the effect of the flavonóides quercetin, naringenin and ipriflavon in the prevention of the emergence of that tumor, as well as the quercetin and ipriflavon to treat the mice with TAE and to impede the growth of the cells of that tumor in vitro. The mice were treated with 10mg/kg of the flavonoids by 20 days. In the 21o day, the animals were inoculated by road intraperitoneal with 1,66x104 cells in PBS pH 7,4. In the experiment that sought the treatment of the tumor, the inoculation of the cells it preceded the daily use of the flavonóides quercetin and ipriflavon. After 18 days of inoculation of the cells, it was collected blood of the animals and all liquid ascítico formed in the abdominal cavity. The levels of total proteins, of the enzymes aspartato aminotransferase (AST) and alanina aminotranferase (ALT) and albumin was measured using enzymatic kits and for count of the cells it used Hemocitometro of Newbauer using the exclusion technique for the tripan blue. In the experiment that aimed at the prevention, increase was observed in the medium values of total proteins of the animals using the naringenin; reduction of the activity of AST when used the ipriflavon and of ALT when associated the quercetin + naringenin. The use of the quercetin, naringenin and quercetin+naringenin provoked an increase in the medium values of albumin. It happened a reduction in the viability of the cells present tumorais in the liquid ascítico with the use of all the tested flavonóides. The animals with TAE treated with the quercetin they presented increase in the activity of AST and in the albumin concentration. When agreements with the ipriflavon, there was a reduction in the activity of ALT. With relationship to the viability of the cells tumorais, there was a reduction, independent of the used flavonóides. The studies in vitro showed that with the increase of the concentration of the flavonóides quercetin and ipriflavon there was a smaller cellular viability. And when the animals were previously inoculated with the cells of TAE treated with 100µM of quercetin and ipriflavon, there was an increase in the medium values of total proteins and albumin in the sanguine serum of the animals and a reduction in the activity of the enzymes AST and ALT when the inoculated cells were treated with the quercetin. The flavonóides quercetin and naringenin possess an activity significant antitumoral in agreement with the criterion of NCI, where it is ended that your use as preventive it should be indicated.