Effect of different vitamin D3 metabolites on intestinal calcium homeostasisrelated gene expression in broiler chickens

Detalhes bibliográficos
Autor(a) principal: Hsiao, Felix Shih-Hsiang
Data de Publicação: 2018
Outros Autores: Cheng, Yeong-Hsiang, Han, Jin-Cheng, Chang, Ming-Huang, Yu, Yu-Hsiang
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: https://locus.ufv.br//handle/123456789/31494
https://doi.org/10.1590/rbz4720170015
Resumo: The purpose of this study was to investigate the effects of vitamin D3 metabolites 1α-hydroxycholecalciferol (1α(OH)D3 ), 25-hydroxycholecalciferol (25(OH)2 D3 ), and 1,25-dihydroxycholecalciferol (1,25(OH)2 D3 ) on growth performance, bone quality, and intestinal calcium homeostasis-related gene expression in broiler chickens. One-day-old broilers were fed a basal diet and basal diet containing different vitamin D3 metabolites. The body weight, feed intake, and feed conversion ratio in control and experimental broilers were measured to assess the growth performance, mineral levels, and bone breaking strength. The duodenum was used to assess calcium homeostasis-related gene expressions by quantitative reverse transcription-PCR. No statistically significant difference was found in growth performance, mineral deposition, or bone breaking strength in broiler chickens after three weeks feeding with vitamin D3 . However, supplementation of vitamin D3 metabolites tended to improve feed conversion rate, bone mineral deposition, and breaking strength in broiler chickens. The results demonstrated that vitamin D3 metabolites significantly upregulated calcium homeostasis-related genes, including calbindin, β-glucuronidase, TRPV6, and Na/Pi IIb cotransporter, mRNA levels after 12 h of feeding. The vitamin D3 metabolite 1,25(OH)2 D3 was the most effective at regulating calcium homeostasis-associated gene expression after 6 h of feeding. Dietary vitamin D3 metabolites may alleviate the development of TD in broiler chickens and these effects probably occur through regulation of intestinal calcium homeostasis-related gene expression
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spelling Hsiao, Felix Shih-HsiangCheng, Yeong-HsiangHan, Jin-ChengChang, Ming-HuangYu, Yu-Hsiang2023-09-14T10:13:08Z2023-09-14T10:13:08Z2018-05-111806-9290https://locus.ufv.br//handle/123456789/31494https://doi.org/10.1590/rbz4720170015The purpose of this study was to investigate the effects of vitamin D3 metabolites 1α-hydroxycholecalciferol (1α(OH)D3 ), 25-hydroxycholecalciferol (25(OH)2 D3 ), and 1,25-dihydroxycholecalciferol (1,25(OH)2 D3 ) on growth performance, bone quality, and intestinal calcium homeostasis-related gene expression in broiler chickens. One-day-old broilers were fed a basal diet and basal diet containing different vitamin D3 metabolites. The body weight, feed intake, and feed conversion ratio in control and experimental broilers were measured to assess the growth performance, mineral levels, and bone breaking strength. The duodenum was used to assess calcium homeostasis-related gene expressions by quantitative reverse transcription-PCR. No statistically significant difference was found in growth performance, mineral deposition, or bone breaking strength in broiler chickens after three weeks feeding with vitamin D3 . However, supplementation of vitamin D3 metabolites tended to improve feed conversion rate, bone mineral deposition, and breaking strength in broiler chickens. The results demonstrated that vitamin D3 metabolites significantly upregulated calcium homeostasis-related genes, including calbindin, β-glucuronidase, TRPV6, and Na/Pi IIb cotransporter, mRNA levels after 12 h of feeding. The vitamin D3 metabolite 1,25(OH)2 D3 was the most effective at regulating calcium homeostasis-associated gene expression after 6 h of feeding. Dietary vitamin D3 metabolites may alleviate the development of TD in broiler chickens and these effects probably occur through regulation of intestinal calcium homeostasis-related gene expressionengBrazilian Journal of Animal ScienceR. Bras. Zootec., 47:e20170015, 2018Creative Commons Attribution Licenseinfo:eu-repo/semantics/openAccessgene1α-hydroxycholecalciferol25-hydroxycholecalciferol25-hydroxycholecalciferolEffect of different vitamin D3 metabolites on intestinal calcium homeostasisrelated gene expression in broiler chickensinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlereponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVLICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/31494/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52ORIGINAL1806-9290-rbz-rbz4720170015.pdf1806-9290-rbz-rbz4720170015.pdfartigoapplication/pdf1198711https://locus.ufv.br//bitstream/123456789/31494/1/1806-9290-rbz-rbz4720170015.pdf2ddcfc4251eb912964d368b9843831f4MD51123456789/314942023-09-14 07:13:09.174oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452023-09-14T10:13:09LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.en.fl_str_mv Effect of different vitamin D3 metabolites on intestinal calcium homeostasisrelated gene expression in broiler chickens
title Effect of different vitamin D3 metabolites on intestinal calcium homeostasisrelated gene expression in broiler chickens
spellingShingle Effect of different vitamin D3 metabolites on intestinal calcium homeostasisrelated gene expression in broiler chickens
Hsiao, Felix Shih-Hsiang
gene
1α-hydroxycholecalciferol
25-hydroxycholecalciferol
25-hydroxycholecalciferol
title_short Effect of different vitamin D3 metabolites on intestinal calcium homeostasisrelated gene expression in broiler chickens
title_full Effect of different vitamin D3 metabolites on intestinal calcium homeostasisrelated gene expression in broiler chickens
title_fullStr Effect of different vitamin D3 metabolites on intestinal calcium homeostasisrelated gene expression in broiler chickens
title_full_unstemmed Effect of different vitamin D3 metabolites on intestinal calcium homeostasisrelated gene expression in broiler chickens
title_sort Effect of different vitamin D3 metabolites on intestinal calcium homeostasisrelated gene expression in broiler chickens
author Hsiao, Felix Shih-Hsiang
author_facet Hsiao, Felix Shih-Hsiang
Cheng, Yeong-Hsiang
Han, Jin-Cheng
Chang, Ming-Huang
Yu, Yu-Hsiang
author_role author
author2 Cheng, Yeong-Hsiang
Han, Jin-Cheng
Chang, Ming-Huang
Yu, Yu-Hsiang
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Hsiao, Felix Shih-Hsiang
Cheng, Yeong-Hsiang
Han, Jin-Cheng
Chang, Ming-Huang
Yu, Yu-Hsiang
dc.subject.eng.fl_str_mv gene
1α-hydroxycholecalciferol
25-hydroxycholecalciferol
25-hydroxycholecalciferol
topic gene
1α-hydroxycholecalciferol
25-hydroxycholecalciferol
25-hydroxycholecalciferol
description The purpose of this study was to investigate the effects of vitamin D3 metabolites 1α-hydroxycholecalciferol (1α(OH)D3 ), 25-hydroxycholecalciferol (25(OH)2 D3 ), and 1,25-dihydroxycholecalciferol (1,25(OH)2 D3 ) on growth performance, bone quality, and intestinal calcium homeostasis-related gene expression in broiler chickens. One-day-old broilers were fed a basal diet and basal diet containing different vitamin D3 metabolites. The body weight, feed intake, and feed conversion ratio in control and experimental broilers were measured to assess the growth performance, mineral levels, and bone breaking strength. The duodenum was used to assess calcium homeostasis-related gene expressions by quantitative reverse transcription-PCR. No statistically significant difference was found in growth performance, mineral deposition, or bone breaking strength in broiler chickens after three weeks feeding with vitamin D3 . However, supplementation of vitamin D3 metabolites tended to improve feed conversion rate, bone mineral deposition, and breaking strength in broiler chickens. The results demonstrated that vitamin D3 metabolites significantly upregulated calcium homeostasis-related genes, including calbindin, β-glucuronidase, TRPV6, and Na/Pi IIb cotransporter, mRNA levels after 12 h of feeding. The vitamin D3 metabolite 1,25(OH)2 D3 was the most effective at regulating calcium homeostasis-associated gene expression after 6 h of feeding. Dietary vitamin D3 metabolites may alleviate the development of TD in broiler chickens and these effects probably occur through regulation of intestinal calcium homeostasis-related gene expression
publishDate 2018
dc.date.issued.fl_str_mv 2018-05-11
dc.date.accessioned.fl_str_mv 2023-09-14T10:13:08Z
dc.date.available.fl_str_mv 2023-09-14T10:13:08Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://locus.ufv.br//handle/123456789/31494
dc.identifier.issn.none.fl_str_mv 1806-9290
dc.identifier.doi.pt-BR.fl_str_mv https://doi.org/10.1590/rbz4720170015
identifier_str_mv 1806-9290
url https://locus.ufv.br//handle/123456789/31494
https://doi.org/10.1590/rbz4720170015
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartofseries.pt-BR.fl_str_mv R. Bras. Zootec., 47:e20170015, 2018
dc.rights.driver.fl_str_mv Creative Commons Attribution License
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Creative Commons Attribution License
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Brazilian Journal of Animal Science
publisher.none.fl_str_mv Brazilian Journal of Animal Science
dc.source.none.fl_str_mv reponame:LOCUS Repositório Institucional da UFV
instname:Universidade Federal de Viçosa (UFV)
instacron:UFV
instname_str Universidade Federal de Viçosa (UFV)
instacron_str UFV
institution UFV
reponame_str LOCUS Repositório Institucional da UFV
collection LOCUS Repositório Institucional da UFV
bitstream.url.fl_str_mv https://locus.ufv.br//bitstream/123456789/31494/2/license.txt
https://locus.ufv.br//bitstream/123456789/31494/1/1806-9290-rbz-rbz4720170015.pdf
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